ELECTROCHIMICA ACTA
·
2020
·
IF 6.215
Dongmiao Qin,Xiaohua Jiang,Guichun Mo,Jinsu Feng,Biyang Deng
A label-free electrochemiluminescence (ECL) immunosensor is developed to detect alpha-fetoprotein (AFP). Ru (bpy) 3 2+ -doped silica nanoparticles (Ru–SiO 2 ) and gold nanoparticle-modified reduced graphene oxide (rGO@Au) are integrated to form an rGO@Au@Ru–SiO 2 composite. The composite is employed as a carrier for immobilizing the target antibody, and a label-free ECL immunosensor is constructed via antigen–antibody interaction. Owing to the numerous Ru (bpy) 3 2+ molecules in the Ru–SiO 2 , a high ECL signal is acquired. With excellent conductivity and a large surface area, the rGO@Au composite not only accelerates electron transfer but also improved the ECL signal and captures antibodies, thus enhancing ECL response and sensitivity. Moreover, boron nitride quantum dots (BNQDs) are used as rGO@Au@Ru–SiO 2 coreactants, achieving high ECL intensity. The proposed ECL system exhibits significant potential in bioanalysis , which is attributed to the excellent biocompatibility and good stability of BNQDs. Under optimal experimental conditions, the sensor has a linear range of 0.0001 ng mL −1 to 100 ng mL −1 and a limit of detection equal to 0.03 pg mL −1 (S/N = 3). The proposed technique is successfully applied in the susceptible detection of AFP in human serum. The recovery ranges from 99% to 100%, and the RSD is less than 2.8%. The constructed ECL immunosensor exhibits acceptable performance during actual application.
研究领域:
生物分析
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2019
·
IF 4.959
Xiaodong Zhu,Yuxue Dai,Yuanling Sun,Hao Liu,Weiyan Sun,Yanna Lin,Dandan Gao,Rui Han,Xueying Wang,Chuannan Luo
The concentration of alpha-fetoprotein (AFP) rises greatly in patients with liver cancer and it is a challenge to construct a sensitive AFP detection method with wide range. Therefore, an easy and label-free sensing electrochemical platform for AFP detection with wide concentration range had been designed in this work. Firstly, MnO 2 functionalized mesoporous carbon hollow sphere (MCHS@MnO 2 ) with optimal performance was synthesized by regulating experimental conditions and characterized by scanning electron microscope (SEM), high resolution transmission electron microscopy (HRTEM), x-ray diffraction (XRD), electrochemical impedance spectroscopy (EIS), and cyclic voltammetry (CV), etc. Then, it was immobilized on glassy carbon electrode (MCHS@MnO 2 /GCE) to build an immunosensor for the detection of AFP. The MCHS@MnO 2 /GCE can catalyze decomposition of H 2 O 2 to generate electrochemical signal, and the signal will decrease after capturing AFP. Due to good electrocatalytic activity of MCHS@MnO 2 to H 2 O 2 , the immunosensor achieved indirect detection of AFP with wide sensing range from 0.10 ng mL −1 to 420 ng mL −1 and a detection limit of 0.03 ng mL −1 . Furthermore, the method had been proven to be satisfactory selectivity and reproducibility, and it was successfully applied to determine the content of AFP in human serum samples with satisfactory results. This method is expected to be used for early diagnosis and prognosis examination of liver cancer patients.
研究领域:
生物医学工程
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肿瘤学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jiayi Wu,Lujie Wang,Hongxin Wang,Xinhui Gu,Yucheng Zhou,Fengna Xi
Sensitive detection of alpha-fetoprotein (AFP) is crucial for early liver cancer screening. In this work, an immunosensor is constructed by combining the immunorecognition interface and enhanced electrochemiluminescence (ECL) of luminol under neutral condition utilizing cobalt oxide (Co 3 O 4 ) nanozymes confined in nanochannels, which realizes highly sensitive detection of AFP. Indium tin oxide (ITO) conductive glass is used as the supporting electrode, and silica nanochannel film (SNF) is simply grown on the ITO surface using the Stöber method. Co 3 O 4 nanozymes are in situ grown within the SNF nanochannels by a one-step electrodeposition method. The rigid 2D nanochannel array of SNF effectively confines the Co 3 O 4 nanozyme, significantly enhancing its stability. Epoxy modification on the external surface of SNF enables covalent attachment of AFP antibodies, leading to recognition interface. Confined Co 3 O 4 exhibits peroxidase-like activity, catalyzing the generation of reactive oxygen species (ROS) from hydrogen peroxide (H 2 O 2 ), enhancing luminol oxidation and boosting the ECL signal under neutral conditions. Upon AFP binding, antibodies capture AFP to form immunocomplexes, causing reduced diffusion of luminol and H 2 O 2 to the electrode surface, decreasing the ECL signal. The constructed immunosensor exhibits a linear range for AFP detection from 100 fg/mL to 10 ng/mL, with a limit of detection (LOD) of 18.5 fg/mL. AFP detection in serum samples from liver cancer patients or healthy individuals is successfully achieved.
研究领域:
生物医学工程
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分析化学
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癌症诊断
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Yicheng Wei,Yan Li,Na Li,Yong Zhang,Tao Yan,Hongmin Ma,Qin Wei
In the present work, an ultrasensitive sandwich-type electrochemical immunosensor based on a novel signal amplification strategy was designed for quantitative detection of alpha fetoprotein (AFP). Au nanoparticles with biocompatibility were electrodeposited on the surface of glassy carbon electrode (GCE) which can effectively capture and immobilize primary anti-AFP (Ab 1 ) to significantly amplify the electrochemical signal. Graphene Oxide and CeO 2 mesoporous nanocomposite functionalized by the 3-aminopropyltriethoxysilane supported Pd octahedral nanoparticles (Pd/APTES-M-CeO 2 -GS) were utilized as labels of detection anti-AFP (Ab 2 ). Pd octahedral nanoparticles presented good catalytic activity towards the reduction of H 2 O 2 . Due to the large specific surface area and good adsorption properties of APTES-CeO 2 -GS nanocomposite, large amount of Pd octahedral nanoparticles could be immobilized, which could amplify the electrochemical signal and improve the sensitivity of the immunosensor. Under optimal conditions, the immunosensor exhibited wide linear range from 0.1 pg/mL to 50 ng/mL with a low detection limit of 0.033 pg/mL ( S / N =3) for AFP detection. In addition, high sensitivity, excellent selectivity, good reproducibility and stability were obtained for the immunosensor, which has a promising application for quantitative detection of other tumor markers in clinical diagnosis.
研究领域:
生物医学工程
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肿瘤标志物检测
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纳米技术应用
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 5
·526-532
·
IF 2.073
Jing Wang,Dongcheng Yang,Mifa Chen,Beifang Liu,Hou Chen,Hui Xu,Wenxiang Wang,Liangjiu Bai
A novel strategy based on nanoprobes for signal amplification was introduced in an electrochemical immunoassay platform. The nanoprobes based on poly(2-hydroxyethyl methacrylate)-r-poly(acrylic acid) (PHEMA-r-PAA) and graphene oxide (GO) have the effect of double signal amplification. The resulting sandwich-type electrochemical immunosensor was able to detect the tumor marker alpha fetal protein (AFP) with high sensitivity and high selectivity. A good linear relationship could be observed in the AFP concentration range of 3.5 pg mL−1 to 35 ng mL−1 (R2 = 0.9898), and the minimum detection limit was 0.106 pg mL−1. In addition, the electrochemical immunosensor exhibited good stability and could be applied to AFP detection in clinical serum.
研究领域:
生物化学
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分析化学
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纳米技术
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癌症研究
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Lei Jiao,Zonggang Mu,Chengzhou Zhu,Qin Wei,He Li,Dan Du,Yuehe Lin
A dual enhancing strategy has been employed to develop electrochemical immunosensor for the ultrasensitive detection of AFP, which is enhanced by poly-dopamine functionalized N-doped multi-walled carbon nanotube (PDA-N-MWCNT) and the nanocomposite of graphene loaded Au@Pt mesoporous nanodendrites. The fabricated immunosensor exhibited a wide linear range of 0.1 pg/mL–10 ng/mL, with an ultralow detection limit of 0.05 pg/mL. The good performance of the developed immunosensor could be contributed to (1) PDA-N-MWCNT supplied good biocompatible surface for immobilizing primary antibody (Ab 1 ); (2) PDA-N-MWCNT modified electrode endowed more electrochemical active area, which can effectively improve the sensitivity of the immunosensor; (3) graphene could load high amount of good conductive and high electrocatalytic active Au@Pt nanodendrites for signal amplification . The results imply that the as-fabricated immunosensors have great potential for clinic usage.
研究领域:
分析化学
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生物化学
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纳米技术
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 5
·1-9
·
IF 5.479
Li Guiyin,Zeng Junxiang,Liu Huiling,Ding Ping,Liang Jintao,Nie Xinmin,Zhou Zhide
Alpha-fetoprotein (AFP) is a reliable clinical marker of hepatocellular carcinoma (HCC). A highly sensitive fluorometric aptamer nanoprobe is described for AFP detection. It is based on fluorescence resonance energy transfer (FRET) between AFP aptamer labelled with 5-carboxyfluorescein (FAM) and palladium nanoparticles (PdNPs). The PdNPs quench the green fluorescence of the FAM-AFP aptamer via interactions between nitrogen functional groups of the AFP aptamer and PdNPs. When AFP was introduced into the FAM-AFP aptamer-PdNPs FRET system, the AFP aptamer preferentially combines with AFP. This results in a conformational change and weakens the interaction between the aptamer and the PdNPs. Thus, the fluorescence of FAM recovers. The fluorescence recovery of FAM increases linearly in the 5.0–150 ng·mL −1 AFP concentration range and has a 1.4 ng·mL −1 detection limit. The assay was applied to the analysis of spiked diluted human serum. The recovery values ranged from 98.3 to 112.9%, with relative standard deviations of <1.1%. This biosensing strategy provides a reliable and ultrasensitive protocol for the quantification of biomarkers with relevant antigens and aptamers.
Schematic presentation of a fluorometric aptamer nanoprobe for AFP assay based on fluorescence resonance energy transfer (FRET) between AFP aptamer labelled with 5-carboxyfluorescein (FAM) and palladium nanoparticles (PdNPs).
研究领域:
生物化学
·
纳米技术
·
癌症研究
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）