Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 40
·6187-6194
·
IF 5.047
Run-tian Ma,Xiao-bo Zhao,Xiao-yu Sun,Jia Liu,Wei Ha,Yan-ping Shi
MIT is a promising strategy in antibody free analysis for tumour markers. Conventional nanosized MIPs with off-line analysis are beset by tedious operation and unsatisfactory analysis performance. In this work, an on-line analytical device to directly detect AFP, which is a typical tumour marker in cancer screening, was prepared for the first time. A microscope slide was chosen to be the basis of the device. APBA–PA, a polymerizable fluorescent boronic acid monomer, was synthesised and grafted on the surface of the microscope slide to act as the signal transduction pathway between the templates and the device. Along with the hydrolysis of TEOS and the elution of the templates, a portable, stable, easy to operate and low-cost analysis device for AFP with excellent repeatability was successfully prepared. Owing to the excellent selectivity and highly sensitive fluorescence response ability of the device towards the templates, the on-line detection of AFP in human serum was realized. A series of characterizations were applied to the device, and its analysis performance and possible detection mechanism were carefully studied. Furthermore, the device exhibited appropriate application prospects by comparing its analysis results with those of the commercially available ELISA. In our perception, this work is an important step towards MIPs for clinical applications.
研究领域:
生物分析化学
·
医学诊断
✓ 已验证
DOI
PubMed
高影响因子
Journal of Cleaner Production
·
2024
·
IF 9.7
Wanru Feng,Jingya Zhang,Jianguo Mi,Junsu Jin,Guolei Xiang,Hong Meng,Shisen Xu
The synthesis of structured CO 2 adsorbents with high separation selectivity, strong stability, and low cost to meet industrial needs is very challenging. Here, we synthesized an ultra-stable Zn-MOF (Zn 2 (3-amion-1,2,4-triazolate) 2 (oxalate), Zn 2 (Atz) 2 Ox), and adopted an efficient in-situ growth strategy to produce millimeter-sized spherical Zn 2 (Atz) 2 Ox@AFP (Amino-Functionalization Poly(acrylates), AFP) composite. Due to the heterogeneous crystallization induced by the nanoscale crystal seeds and the confined space of AFP, the three-dimensional (3D) Zn 2 (Atz) 2 Ox bulk crystals transformed into 2D nanosheets with higher CO 2 adsorption efficiency and faster kinetics. Zn 2 (Atz) 2 Ox@AFP has a high affinity for CO 2 (2.05 mmol g −1 at 0.05 bar and 313 K) while exhibiting an inhibitory effect for N 2 (only 0.051 mmol g −1 at 1.0 bar and 313 K), leading to an excellent ideal adsorbed solution theory (IAST) selectivity (2990 for CO 2 /N 2 (5/95, v/v)). Interestingly, Zn 2 (Atz) 2 Ox@AFP exhibits a unique gating effect on CH 4 adsorption, which further extends its application range to natural gas purification. Dynamic breakthrough experiments confirmed that Zn 2 (Atz) 2 Ox@AFP can selectively capture CO 2 in the simulated CO 2 /N 2 (5/95) and CO 2 /CH 4 (5/95) mixed gases.
研究领域:
材料科学
·
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Nanomaterials
·
2025
·Vol. 15
·期 8
·612
·
IF 4.3
Kang Yang,Fang Yang,Xiaoling Lu,Hao Li,Zeng Yang,Qi Yin,Lin Zhang,You Long,Chao Shen,Liya Chen,Bo Yao,Chenghong Huang
Simple and accurate analysis of cancer-related biomarkers is very important for disease screening and auxiliary diagnosis. This study proposed a facile immunoassay that used gold nanostar-labeled rabbit anti-AFP as a capture antibody and gold nanoparticle-conjugated goat anti-rabbit IgG as an enhance antibody for the construction of a detection strategy for AFP analysis. Investigations indicated that the 50 nm diameter GNS-labeled capture antibody can specifically catch AFPs by direct detection profile or by further signal amplification through AuNP-tagged enhance antibody combination. Results showed that the developed method holds 8.6 ng/mL sensitivity, 20.0–110.0 ng/mL detection range, acceptable precision and fine accuracy, as well as favorable specificity. Results of application to real serum determination by the proposed method are highly related to those of the ECLIA method (correlation coefficient is 0.931). The proposed method has simple-operation merit and is very suitable for clinical screening of large-scale serum samples of cancers.
研究领域:
医学诊断
·
纳米生物学
·
临床检验
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Lei Jiao,Zonggang Mu,Chengzhou Zhu,Qin Wei,He Li,Dan Du,Yuehe Lin
A dual enhancing strategy has been employed to develop electrochemical immunosensor for the ultrasensitive detection of AFP, which is enhanced by poly-dopamine functionalized N-doped multi-walled carbon nanotube (PDA-N-MWCNT) and the nanocomposite of graphene loaded Au@Pt mesoporous nanodendrites. The fabricated immunosensor exhibited a wide linear range of 0.1 pg/mL–10 ng/mL, with an ultralow detection limit of 0.05 pg/mL. The good performance of the developed immunosensor could be contributed to (1) PDA-N-MWCNT supplied good biocompatible surface for immobilizing primary antibody (Ab 1 ); (2) PDA-N-MWCNT modified electrode endowed more electrochemical active area, which can effectively improve the sensitivity of the immunosensor; (3) graphene could load high amount of good conductive and high electrocatalytic active Au@Pt nanodendrites for signal amplification . The results imply that the as-fabricated immunosensors have great potential for clinic usage.
研究领域:
分析化学
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生物化学
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纳米技术
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2020
·
IF 6.215
Dongmiao Qin,Xiaohua Jiang,Guichun Mo,Jinsu Feng,Biyang Deng
A label-free electrochemiluminescence (ECL) immunosensor is developed to detect alpha-fetoprotein (AFP). Ru (bpy) 3 2+ -doped silica nanoparticles (Ru–SiO 2 ) and gold nanoparticle-modified reduced graphene oxide (rGO@Au) are integrated to form an rGO@Au@Ru–SiO 2 composite. The composite is employed as a carrier for immobilizing the target antibody, and a label-free ECL immunosensor is constructed via antigen–antibody interaction. Owing to the numerous Ru (bpy) 3 2+ molecules in the Ru–SiO 2 , a high ECL signal is acquired. With excellent conductivity and a large surface area, the rGO@Au composite not only accelerates electron transfer but also improved the ECL signal and captures antibodies, thus enhancing ECL response and sensitivity. Moreover, boron nitride quantum dots (BNQDs) are used as rGO@Au@Ru–SiO 2 coreactants, achieving high ECL intensity. The proposed ECL system exhibits significant potential in bioanalysis , which is attributed to the excellent biocompatibility and good stability of BNQDs. Under optimal experimental conditions, the sensor has a linear range of 0.0001 ng mL −1 to 100 ng mL −1 and a limit of detection equal to 0.03 pg mL −1 (S/N = 3). The proposed technique is successfully applied in the susceptible detection of AFP in human serum. The recovery ranges from 99% to 100%, and the RSD is less than 2.8%. The constructed ECL immunosensor exhibits acceptable performance during actual application.
研究领域:
生物分析
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医学诊断
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Qiang Chu,Meng Chen,Dongxiao Song,Xixi Li,Yunyun Yang,Zihuan Zheng,Yonglu Li,Yangyang Liu,Lushuang Yu,Zheng Hua,Xiaodong Zheng
Oxidative stress is a critical cause for the progression of neurodegenerative diseases, indicating that screening natural antioxidant and pharmacological targeting of mechanism need more attention. Natural plant polysaccharides are the primary group of natural antioxidants showing mighty antioxidant activity. Therefore, a polysaccharide (AFP-2) from flowers of Apios americana Medik was isolated, purified and characterized. Then, a H 2 O 2 -induced PC12 cells oxidative stress model was established to investigate the neuroprotective role of AFP-2 and to clarify the potential mechanism of action against H 2 O 2 -induced neurotoxicity. It was found that AFP-2 reduced ROS production and mitochondrial damage caused by hydrogen peroxide in PC12 cells. In addition, data from transmission electron microscope and western blot displayed that AFP-2 significantly activated autophagy in PC12 cells via Akt-mTOR pathway. These results together qualify AFP-2 as an interesting natural polysaccharide worth further investigation as a neuroprotective agent.
研究领域:
神经科学
·
药理学
·
分子生物学
·
天然产物化学
✓ 已验证
DOI
PubMed
高影响因子
Biomaterials Science
·
2022
·Vol. 10
·期 14
·3823-3830
·
IF 7.59
Ke Lu,Chaoran Liu,Gaofeng Wang,Weihuang Yang,Kai Fan,Serguei Lazarouk,Vladimir Labunov,Linxi Dong,Dujuan Li,Xun Yang
Liver cancer is one of the malignant tumors with the highest fatality rate and increasing incidence, which has no effective treatment plan. Early diagnosis and early treatment of liver cancer play a vital role in prolonging the survival period of patients and improving the cure rate. Carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) are two crucial tumor markers for liver cancer diagnosis. In this work, we firstly proposed a wafer-level, highly controlled silicon nanowire (SiNW) field-effect transistor (FET) joint detection sensor for highly sensitive and selective detection of CEA and AFP. The SiNWs-FET joint detection sensor possesses 4 sensing regions. Each sensing region consists of 120 SiNWs arranged in a 15 × 8 array. The SiNW sensor was developed by using a wafer-level and highly controllable top-down manufacturing technology to achieve the repeatability and controllability of device preparation. To identify and detect CEA/AFP, we modified the corresponding CEA antibodies/AFP antibodies to the sensing region surface after a series of surface modification processes, including O2 plasma treatment, soaking in 3-aminopropyltriethoxysilane (APTES) solution, and soaking in glutaraldehyde (GA) solution. The experimental results showed that the SiNW array sensor has superior sensitivity with a real-time ultralow detection limit of 0.1 fg ml−1 (AFP in 0.1× PBS) and 1 fg ml−1 (CEA in 0.1× PBS). Also, the logarithms of the concentration of CEA (from 1 fg ml−1 to 10 pg ml−1) and AFP (from 0.1 fg ml−1 to 100 pg ml−1) achieved conspicuously linear relationships with normalized current changes. The R2 of AFP in 0.1× PBS and R2 of CEA in 0.1× PBS were 0.99885 and 0.99677, respectively. Furthermore, the sensor could distinguish CEA/AFP from interferents at high concentrations. Importantly, even in serum samples, our sensor could successfully detect CEA/AFP. This demonstrates the promising clinical development of our sensor.
研究领域:
生物医学工程
·
癌症研究
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纳米技术
·
生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Yicheng Wei,Yan Li,Na Li,Yong Zhang,Tao Yan,Hongmin Ma,Qin Wei
In the present work, an ultrasensitive sandwich-type electrochemical immunosensor based on a novel signal amplification strategy was designed for quantitative detection of alpha fetoprotein (AFP). Au nanoparticles with biocompatibility were electrodeposited on the surface of glassy carbon electrode (GCE) which can effectively capture and immobilize primary anti-AFP (Ab 1 ) to significantly amplify the electrochemical signal. Graphene Oxide and CeO 2 mesoporous nanocomposite functionalized by the 3-aminopropyltriethoxysilane supported Pd octahedral nanoparticles (Pd/APTES-M-CeO 2 -GS) were utilized as labels of detection anti-AFP (Ab 2 ). Pd octahedral nanoparticles presented good catalytic activity towards the reduction of H 2 O 2 . Due to the large specific surface area and good adsorption properties of APTES-CeO 2 -GS nanocomposite, large amount of Pd octahedral nanoparticles could be immobilized, which could amplify the electrochemical signal and improve the sensitivity of the immunosensor. Under optimal conditions, the immunosensor exhibited wide linear range from 0.1 pg/mL to 50 ng/mL with a low detection limit of 0.033 pg/mL ( S / N =3) for AFP detection. In addition, high sensitivity, excellent selectivity, good reproducibility and stability were obtained for the immunosensor, which has a promising application for quantitative detection of other tumor markers in clinical diagnosis.
研究领域:
生物医学工程
·
肿瘤标志物检测
·
纳米技术应用
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Yu Liu,Gaohua Han,Jing Gong,Xin Hua,Qian Zhu,Sisi Zhou,Ling Jiang,Quan Li,Songqin Liu
Early and accurate diagnosis of hepatocellular carcinoma (HCC) is of significant importance for improving the survival rate and quality of life for HCC patients. The combined detection of alpha-fetoprotein (AFP) and alpha-fetoprotein-L3 (AFP-L3), namely AFP-L3%, can greatly improve the accuracy of HCC diagnosis compared with AFP detection. Herein, we developed a novel intramolecular fluorescence resonance energy transfer (FRET) strategy for sequential detection of AFP and AFP-specific core fucose to improve the diagnosis accuracy of HCC. Firstly, fluorescence-labeled AFP aptamer (AFP Apt-FAM) was used to specifically recognize all AFP isoforms, and total AFP was quantitatively determined using fluorescence intensity of FAM. Then, 4-((4-(dimethylamino)phenyl)azo)benzoic acid (Dabcyl) labeled lectins (PhoSL-Dabcyl) were used to specifically recognize the core fucose expressed on AFP-L3 that does not bind to other AFP isoforms. The combination of FAM and Dabcyl on the same AFP molecule could generate FRET effect, thereby quenching the fluorescence signal of FAM and quantitatively determining AFP-L3. After that, AFP-L3% was calculated according to the ratio of AFP-L3 to AFP. With this strategy, the concentration of total AFP, AFP-L3 isoform as well as the AFP-L3% were sensitively detected. Detection limits of 0.66 and 0.186 ng/mL were obtained for AFP and AFP-L3 in human serum, respectively. Clinical human serum test results showed that AFP- L3 % test was more accurate than AFP assay to distinguish healthy people, HCC patients and benign liver disease patients. Therefore, the proposed strategy is simple, sensitive and selective, which can improve the accuracy of early diagnosis of HCC, and has good clinical application potential.
研究领域:
医学诊断
·
癌症研究
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Analytical Methods
·
2016
·Vol. 8
·期 40
·7319-7323
·
IF 1.915
Jisan Sun,Dazhi Tian,Qingjun Guo,Li Zhang,Wentao Jiang,Minghui Yang
A sensitive and selective electrochemical immunosensor for label-free detection of cancer biomarker α-fetoprotein (AFP) was developed based on hydroxyapatite (HAP) nanoparticles induced electrochemical current. HAP nanoparticles and graphene oxide (GO) were co-immobilized onto an electrode surface to modify the electrode. The phosphate groups contained in the HAP nanoparticles can react with molybdate to form redox molybdophosphate precipitates on the electrode surface, generating electrochemical current. GO enhanced the electrochemical current intensity due to its good conductivity. The following immobilization of the anti-AFP antibody and capture of AFP resulted in the formation of an immuno-complex layer on the electrode. This immuno-complex layer prevented the reaction of hydroxyapatite nanoparticles with molybdate, leading to the decrease of the electrochemical current, and the current change is proportional to the concentration of AFP detected. A wide linear range (0.01–10 ng mL−1) and low detection limit (5 pg mL−1) towards AFP detection were obtained. The reliability of the proposed immunosensor was evaluated by the detection of AFP in human serum samples. This study demonstrated the potential of the developed immunosensor in ultrasensitive detection of AFP or other biomarkers.
研究领域:
生物化学
·
分析化学
·
生物医学工程
·
癌症研究
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 20
·2390-2397
·
IF 2.073
Dongcheng Yang,Jing Wang,Hou Chen,Hui Xu,Wenxiang Wang,Huawei Yang,Donglei Wei,Liangjiu Bai
A sensitive sandwich-type electrochemical immunosensor based on polyacrylonitrile-g-poly(hydroxyethyl methacrylate) (PAN-g-PHEMA)/graphene oxide (GO) composites was prepared for the ultrasensitive detection of tumor markers (alpha fetal protein, AFP as a model). The functional polymer brush was efficiently prepared by the union of cyano click chemistry and controlled/“living” radical polymerization (CRP). The designed immunosensor showed a low limit of detection (LOD, ∼0.285 pg mL−1), with a wide detection range (2.5 × 101 to 2.5 × 104 pg mL−1) (R2 = 0.9876). The electrochemical immunosensor also exhibited high sensitivity, favorable stability, excellent reproducibility and good selectivity towards AFP. Moreover, the immunosensor could be applied to the analysis of AFP in serum samples with satisfactory results. This present work may provide a general model for the detection of tumor markers based on sandwich-type immunosensors.
研究领域:
材料科学
·
分析化学
·
生物传感器
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Biosensors-Basel
·
2022
·Vol. 12
·期 10
·818
·
IF 5.743
Jiali Huang,Xu Li,Mingzhen Xiu,Kang Huang,Kang Cui,Jing Zhang,Shenguang Ge,Shiji Hao,Jinghua Yu,Yizhong Huang
Nowadays, developing a cost-effective, easy-to-operate, and efficient signal amplification platform is of important to microfluidic paper-based analytical devices (μPAD) for end-use markets of point-of-care (POC) assay applications. Herein, an ultrasensitive, paper-based photoelectrochemical (PEC) bioassay platform is constructed by in situ grown ZnO/ZnIn2S4heterojunctions onto paper fibers, which acted as photoactive signal amplification probes for enhancing the sensitivity of antibodies-based diagnostic assays, for the sensitive detection of alpha-fetoprotein (AFP) targets. The crystalline flake-like ZnIn2S4composited with hexagonal nanorods (NRs) morphology of ZnO is an in situ grown, at the first time, onto cellulose fibers surface supported with Au nanoparticle (Au NP) modification to improve conductivity of the device working zone. The obtained composites on paper fibers are implemented as a flexible paper-based photoelectrode to realize remarkable performance of the fabricatedμPAD, resulting from the enhanced PEC activity of heterojunctions with effective electron-hole pair separation for accelerating photoelectric conversion efficiency of the sensing process under light irradiation. Once the target AFP was introduced into the biosensing interface assistant, with a specific recognition interaction of AFP antibody, a drastically photocurrent response was generated, in view of the apparent steric effects. With the concentration increase of AFP targets, more immune conjugates could be confined onto the biosensing interface, eventually leading to the quantitative decrease of photocurrent intensity. Combined with an ingenious origami design and permitting the hydrophobic/hydrophilic conversion procedure in the bioassay process, the ultrasensitive PEC detection of AFP targets was realized. Under the optimized conditions, the level of AFP could be sensitively tracked by the preparedμPAD with a liner range from 0.1 to 100 ng mL−1and limit of detection of 0.03 ng mL−1. This work provides a great potential application for highly selective and sensitive POC testing of AFP, and finally, developments for clinical disease diagnosis.Keywords:μPAD;photoelectrochemical;alpha-fetoprotein;point-of-care assay;immunosensing
研究领域:
生物医学工程
·
分析化学
·
即时诊断
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）