JOURNAL OF MOLECULAR STRUCTURE
·
2024
·
IF 4
Hao Hu,Lei Xue,Yelin Yao,Linlong Deng,Haibin Wang
A novel fluorescent probe, WH , was successfully designed and synthesized. WH demonstrated continuous detection capabilities for Cu 2+ and EDTA in the HEPES system. Specifically, the introduction of copper ions resulted in fluorescence quenching, while the addition of EDTA led to the recovery of fluorescence. The detection limits of WH for Cu 2+ and EDTA were calculated to be 29.66 and 172.13 nmol· L −1 , respectively. Moreover, WH exhibited AIE property in THF/H 2 O system. According to the Job's plot, 1 H NMR titration and mass spectrometry, WH could form a complex with Cu 2+ in a 1:1 ratio, and the combinding constant was 8.2 × 10 3 L mol −1 . WH exhibited outstanding selectivity, high sensitivity, large Stokes shift and strong anti-interference ability. Furthermore, WH had been successfully applied in the detection of Cu 2+ in beer and red wine, showcasing its promising potential for practical application.
研究领域:
分析化学
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材料科学
·
环境科学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Peiyao Wang,Yupeng Lv,Xinhui Hou,Xiaoluan Yang,Qian Tao,Guiying Li
Fluorescent probe with aggregation-induced emission (AIE) property has been widely used because of the advantages of high sensitivity, good selectivity and non-destructive testing. The development of fluorescent probe with good biocompatibility, photostability and biodegradability is of great significance in biomedicine and environmental detection. Herein, a novel type of fluorophore CS-TPE for detection of Fe 3+ and bacteria was prepared by the Schiff base reaction of chitosan (CS) and 4-(1,2,2-triphenylethenyl) benzaldehyde (TPE-CHO). The fluorescence response mechanism of CS-TPE system was investigated by various characterization techniques. CS-TPE had an obvious AIE behavior with strong blue-green emissions at 473 nm and reaches the highest photoluminescence (PL) emission in 90 % H 2 O/ethanol mixtures. CS-TPE fluorescent probe exhibited sensitive quenching response to Fe 3+ , which can be used as a biosensor for detecting the concentration of Fe 3+ with short response time (5 min), low detection limit (0.998 μM) and wide detection range (10–300 μM). Meanwhile, CS-TPE exhibited good antibacterial performance for S. aureus and E. coli . It is expected to realize the real-time fluorescence monitoring of metal ion detection and antibacterial process.
研究领域:
生物医学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Chao Peng,Xinyu Ma,Dan Lin,Xiaowen Feng,Huan Yu,Yinhui Li
Mitophagy , a specialized form of autophagy, holds the key to cellular metabolism and physiology. Viscosity is a significant marker for visualization of the mitophagy process in real-time. Hence, development of well-performing viscosity probe is beneficial to study mitophagy-related dynamic physiological and pathological processes. Here, a new strategy was proposed by combination of AIE property and molecular rotors to design novel viscosity probe. The probe named TPA-Py was obtained by Knoevenagel condensation reaction of AIE unit and pyridine salt, which giving the probe excellent near-infrared emission, good water-solubility and mitochondrial targeting ability. Most importantly, TPA-Py owns two rotatable parts of triphenylamine and double bond , enabling the probe to equip with AIE property and sensitive recognition units for viscosity. With the environmental viscosity increasing, the rotation of the molecular rotor and the AIE unit is restricted effectively, the probe displayed strong fluorescence. Then, TPA-Py was successfully employed for monitoring the mitophagy process in A549 cells by imaging viscosity alterations. As mitophagy constitutes an important consideration in the pathogenesis of drug-induced liver injury, TPA-Py was also applied to explore the variation of viscosity in production and remediation pathways of APAP-induced liver injury. These results demonstrated that TPA-Py was a highly sensitive viscosity probe which holds great potential of biological applications.
研究领域:
生物化学
·
医学影像
·
细胞生物学
✓ 已验证
DOI
高影响因子
Polymer Chemistry
·
2017
·Vol. 8
·期 48
·7486-7498
·
IF 5.375
Bing Wang,Ping Chen,Ji Zhang,Xiao-Chuan Chen,Yan-Hong Liu,Zheng Huang,Qing-Ying Yu,Ju-Hui Zhang,Wei Zhang,Xi Wei,Xiao-Qi Yu
With the aim to develop a novel multifunctional gene delivery system that may overcome the common barriers of gene transfection, two amphiphilic molecules with tetraphenyl ethylene (TPE) as a hydrophobic moiety and linear PEI-galactose as a hydrophilic part were elaborately designed and synthesized. The molecules could self-assemble to form micelles and exhibited aggregation-induced emission (AIE) property, which may be used for cell imaging. Several assays revealed that the disulfide bond in TSPG may facilitate vector decomposition and DNA release, resulting in higher transfection efficiency and lower cytotoxicity than TCPG and PEI 25 kDa. These vectors showed excellent serum tolerance, and even higher efficiency could be obtained in transfection with the presence of serum. Moreover, the transfection in different cell lines showed that TSPG complex could induce higher gene expression in asialoglycoprotein receptor-rich HepG2 cells than in HeLa cells, which lack such receptors. Galactose competition assay also demonstrated that the galactose moiety could afford the vector with targeting ability toward hepatoma cells. Cellular uptake inhibition assay indicated that the DNA complex entered the cell mainly via caveolae-mediated endocytosis. Results showed that TSPG may serve as a promising reagent for simultaneous gene transfection and imaging with good biocompatibility and cell targeting ability.
研究领域:
生物医学工程
·
分子生物学
·
药剂学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2025
·
IF 4.7
Huaqin Jiang,Lingling Shi,Wenxiang Wang,Yang Yang,Min Fang,Weiju Zhu,Cun Li
The peroxynitrite anion (ONOO - ) and viscosity play crucial roles in both physiological and pathological processes. In this study, we developed a bifunctional fluorescent probe MAR with an aggregation-induced emission (AIE) feature for detecting viscosity and ONOO - across different channels. MAR exhibited significant fluorescence enhancement to viscosity with a red emission at 600 nm and a good linear relationship in the range of 11.6 to 956 cP. Additionally, MAR could rapidly recognize and quantitatively respond to ONOO - with high selectivity, good sensitivity, a low detection limit (LOD = 6.97 nM), and a wide range of pH value (4.0 - 10.0), accompanied by visible blue fluorescence emission at 408 nm. The considerable wavelength separation (Δλ = 192 nm) between the maximum emission peaks of MAR for viscosity and ONOO - ensured minimal signal crosstalk in bioimaging. Cell imaging experiments indicated that MAR could target intracellular lipid droplets and respond to the changes of viscosity and ONOO - through independent red and blue channels. These results indicated that the AIEgen fluorescence sensor MAR was a promising candidate for detecting ONOO - levels and viscosity changes in live cells.
研究领域:
生物化学
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医学检测
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Siqi Chen,Ruyan Xie,Miaomiao Tian,Rui Yan,Zizhun Wang,Ruifeng Xie,Fang Chai
Heavy metal pollution is life-threatening, the detection of heavy metals is crucial to human health and environment. In this work, AIE featured LA-stabilized gold nanoclusters (NL-AuNCs) were designed and fabricated by modulating precursors in various reaction conditions, and utilized as an efficient fluorescent sensor for portable detecting Ag + with high sensitivity and selectivity. The NL-AuNCs exhibited intense red fluorescence at 625 nm via the secondary reducing agent N-acetyl-L-cysteine (NAC), achieving a quantum yield (QY) of 15.7 %. Notably, the introduction of Ag + enhanced the red fluorescence intensity of NL-AuNCs on account of the aggregation-induced emission (AIE) process, rendering NL-AuNCs uniquely capable of detecting Ag + with the ultralow detection limit of 1.3 nM within a wide concentration range of 0.002–200 μM. Furthermore, the handheld intelligent sensing strategy was constructed by integrating the smartphone App, which enabled swift portable monitoring and convenient readout by visual assessment of color evolution. The acceptable recoveries of 94.6 % to 114.9 % were attained from testing real water, indicating the excellent environmental tolerance of NL-AuNCs, which allowed the possibility for practical in-situ detection.
研究领域:
环境科学
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2024
·Vol. 12
·期 39
·15903-15914
·
IF 5.7
Mengyuan Niu,Yuliang Yang,Yue Sun,Yu Hu,Kaiyue Song,Xiaoxia Sun
A novel chiral fluorescent probe, tetraphenylvinyltriazole-ribosyl ribose derivative (β,D)-RIB-TPE, was prepared by Click reaction. It can self-assemble into amphiphilic micron vesicles in methanol solution. (β,D)-RIB-TPE has good AIE properties with a large Stokes shift (redshift 61.8 nm), self-assembles into J-aggregates in a mixed solution with 98% water content, and exhibits a relative fluorescence intensity αAIE = 24, emitting bright blue fluorescence. In addition to the micron vesicles, (β,D)-RIB-TPE self-assembled into J-aggregates in different mixtures, showing different morphologies, such as a cross-linked network structure and an irregular bulk stacking structure. The fluorescent probe (β,D)-RIB-TPE could achieve high efficiency and rapid multi-channel detection of clopidogrel S-(+)-sulfate by fluorescence chromatography and CD spectroscopy. Infrared, nuclear magnetic titration, scanning electron microscopy and DFT calculations confirmed that the probe (β,D)-RIB-TPE recognized S-(+)-clopidogrel sulfate by an ICT mechanism. (β,D)-RIB-TPE and S-(+)-clopidogrel sulfate were co-assembled in methanol to form single-target drug-carrying micron vesicles with a smaller particle size than the self-assembled (β,D)-RIB-TPE vesicles and good drug embedding capacity.
研究领域:
材料科学
·
生物医学工程
·
药学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）