TALANTA
·
2025
·
IF 6.1
Ruru Chai,Jing Zeng,Alai Hailaxi,Yueling Song,Zhengchong Shen,Guixin Li
This study presents a highly sensitive cysteine (Cys) sensing platform based on a solvent-responsive aggregation-induced electrochemiluminescence (AIECL) probe. By modulating the DMF/PBS solvent ratio, the aggregation state of tetraphenylethylene derivative (TPE-1-CHO) was optimized, resulting in a significant enhancement of its cathodic electrochemiluminescence (ECL) performance. Experimental results revealed that at an aqueous fraction ( fw ) of 50%, the aggregation degree of TPE-1-CHO and its ECL efficiency reached an optimal balance, yielding an ECL intensity substantially higher than that in pure DMF ( fw = 0%). Based on this phenomenon, an AIECL sensing platform was constructed using K 2 S 2 O 8 as a co-reactant, and the proposed platform can effectively discriminate structurally similar biomolecules based on their reducing functional group. Given its strong ECL suppression, Cys was chosen as the target analyte to assess the analytical characteristics of the presented AIECL sensing strategy. The developed sensor exhibits exceptional sensitivity, outstanding stability, and favorable selectivity. The method was effectively utilized for the quantitative detection of Cys in human serum samples. This study offers an innovative approach for the detection of reducing bioactive small molecules.
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高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Ya Gao,Hao Liu,Shaowei Li,Yao Xiao,Chengyi Xiong,Wei Wen,Shengfu Wang,Xiuhua Zhang,Miao-Miao Chen
Aflatoxins B1 (AFB1), as a secondary metabolite of Aspergillus flavus and Aspergillus parasiticus , is the most toxic mycotoxin. It is urgently needed to detect AFB1 pollution in time at ultra-low concentration levels. Aggregation-induced electrochemiluminescence (AIECL) has aroused widespread interest due to the significantly improved ECL response by overcoming aggregation-caused quenching. However, current AIECL probes to satisfy trace AFB1 determination remain a big challenge. Herein, by virtue of hollow UiO-66-NH 2 with multiple active sites, 1,1,2,2-tetra(4-carboxylphenyl)ethylene (TCTPE)-functionalized AIECL probe (TH-UiO-66-NH 2 ) was designed and prepared for constructing an aptamer-based sensor toward AFB1 monitoring. Due to the coordination of TCTPE and Zr 4+ , TCTPE molecules were loaded and largely restricted in hollow UiO-66-NH 2 , leading to a breakthrough in AIECL signal enhancement. Further combined with magnetic separation and accumulated strand displacement, ultrasensitive and selective detection of AFB1 was achieved with an ultra-low detection limit of 1.04 fg/mL, providing new ideas for food safety supervision.
研究领域:
食品安全
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分析化学
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生物传感
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高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2023
·
IF 4.5
Ziqi Wang,Yafang Gao,Zhangjin He,Liping Lu
In this paper, we systematically investigated the aggregation-induced electrochemiluminescence (AIECL) of the ruthenium(II) complex ([Ru(phen) 2 (phen-O 2 )](PF 6 ) 2 , phen-O 2 : 5,6-diketone-1,10-phenanthroline), which was dissolved in solvents with different water/acetonitrile volume ratios. The fluorescence intensity of the system increased 11.5-fold when the proportion of water (a poor solvent) in the solvent mixture was increased to 90%, and the electrochemiluminescence intensity increased 16-fold when the proportion of water in the solvent was increased to 80%. Transmission electron microscopy and dynamic light scattering confirmed the aggregation of [Ru(phen) 2 (phen-O 2 )](PF 6 ) 2 molecules into nanoclusters. Aggregation-induced electrochemiluminescence properties were observed to increase with an increase in the proportion of water in H 2 O/CH 3 CN. Due to the excellent aggregation-induced luminescence performance of [Ru(phen) 2 (phen-O 2 )](PF 6 ) 2 , the quantitative detection of dopamine was achieved with a good linear range of 100 pM–1uM and a limitation of detection of 33.3 pM. This simple and sensitive method based on the AIECL effect can be widely applied to the detection and analysis of other small biological molecules.
研究领域:
分析化学
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材料科学
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电化学
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纳米技术
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生物传感器
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 414
·期 3
·1389-1402
·
IF 4.157
Lv Xiaoyi,Bi Mengmeng,Xu Xiaoyun,Li Yanping,Geng Chao,Cui Bo,Fang Yishan
Aggregation-induced electrochemiluminescence reagent, a distyrylbenzene derivative with donor–acceptor conjugated nanosheet structure, namely TPAPCN, was used as a trace label and modified on the electrode through the formation of classical sandwich complex of antibody-antigen–antibody in this work. In aggregate state, TPAPCN with twisted structure was limited in nanometer space through intermolecular π − π stacking interactions, which not only restricts the intramolecular motions but also combines a large number of singlet excitons to greatly trigger electrochemiluminescence (ECL). The ECL signal of this system enhanced with more captured cytokeratin 19 fragment 21–1 (CYFRA21-1) on the modified electrode. Three-dimensional graphene/platinum nanoparticles with large specific surface, and excellent electroconductivity and biocompatibility were prepared and acted as excellent carriers for thionine handling (3D-GN/PtNPs/Th), which was employed for improving the loading of antibodies and generating internal electrochemical signal. Consequently, a novel ratiometric sandwich immunosensor for CYFRA21-1 detection was fabricated based on TPAPCN and 3D-GN/PtNPs/Th, that is, a rapid and reliable detection was achieved through the ratio between ECL and electrochemical signals. The prepared sensor performed good linearity in the range of 50 fg/mL to 1 ng/mL with a detection limit as low as 16 fg/mL. Moreover, the detection results revealed well in the analysis of human serum samples, demonstrating a significant application for clinical monitoring and biomolecules detection. Graphical abstract
研究领域:
分析化学
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生物化学
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纳米技术
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生物传感器
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Tingyi Huang,Li Tan,Wenyi Zhang,Ying Ma,Ziqi Lian,Benlin Hong,Jianqiang Hu
Highly sensitive and simple biomarker determination is crucial for diagnosis and treatment of early diseases. However, it remains a tremendous challenge for rapid, accurate and real time detection of biomarkers at the early stage of diseases due to its extremely low content and serious interference. Herein, we have successfully constructed an ultrasensitive electrochemiluminescence (ECL) biosensor based on 2,6-bis(2-benzimidazolyl)pyridine platinum(II) (BZIMPY-Pt) complexes with aggregation-induced electrochemiluminescence (AIECL) phenomenon, which was utilized to detect miRNA-155 at early diseases. The BZIMPY-Pt complexes with AIECL properties and had high affinity with G-quadruplex DNA compared to other square planar pyridyl Pt(II) complexes, which exhibited 13-fold enhancements in ECL intensity in the aggregated state. The constructed biosensor possessed high sensitivity, wide linear range (1 fM-10 nM), low limit of detection (∼0.22 fM), high selectivity and good stability and reproducibility for miRNA-155 detection. The ECL biosensor was successfully applied to determine miRNA-155 in the serum samples of early-stage diagnose. This AIECL-based strategy overcomes traditional limitations in low-abundance biomarker analysis through synergistic signal amplification and background suppression mechanisms, which is potential for clinical diagnosis of early diseases.
研究领域:
生物传感器
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生物分析化学
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纳米技术
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分子诊断学
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电化学
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临床化学
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金属配合物药物
·
生物医学工程
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高影响因子
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