ANALYTICA CHIMICA ACTA
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2024
·
IF 5.7
Yuping Zeng,Xiangyu Gan,Zhourui Xu,Xiaoxiang Hu,Chenxuan Hu,Hanbin Ma,Hangjia Tu,Bao Chai,Chengbin Yang,Siyi Hu,Yujuan Chai
Background Sensitive and rapid antigen detection is critical for the diagnosis and treatment of infectious diseases, but conventional ELISAs including chemiluminescence-based assays are limited in sensitivity and require many operation steps. Fluorescence immunoassays are fast and convenient but often show limited sensitivity and dynamic range. Results To address the need, an aggregation-induced emission fluorgens (AIEgens) enhanced i mmunofluorescent assay with beads-based quantification on the digital microfluidic (DMF) platform was developed. Portable DMF devices and chips with small electrodes were fabricated, capable of manipulating droplets within 100 nL and boosting the reaction efficiency. AIEgen nanoparticles (NPs) with high fluorescence and photostability were synthesized to enhance the test sensitivity and detection range. The integration of AIEgen probes, transparent DMF chip design, and the large magnetic beads (10 μm) as capture agents enabled rapid and direct image-taking and signal calculation of the test result. The performance of this platform was demonstrated by point-of-care quantification of SARS-CoV-2 nucleocapsid (N) protein. Within 25 min, a limit of detection of 5.08 pg mL −1 and a limit of quantification of 8.91 pg mL −1 can be achieved using <1 μL sample. The system showed high reproducibility across the wide dynamic range (10–10 5 pg mL −1 ), with the coefficient of variance ranging from 2.6% to 9.8%. Significance This rapid, sensitive AIEgens-enhanced immunofluorescent assay on the DMF platform showed simplified reaction steps and improved performance, providing insight into the small-volume point-of-care testing of different biomarkers in research and clinical applications.
研究领域:
生物医学工程
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分析化学
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分子诊断
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产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2021
·Vol. 8
·期 9
·2001750
·
IF 16.806
Ying Li,Fei Liu,Jiangjiang Zhang,Xiaoye Liu,Peihong Xiao,Haotian Bai,Shang Chen,Dong Wang,Simon H. P. Sung,Ryan T. K. Kwok,Jianzhong Shen,Kui Zhu,Ben Zhong Tang
Bacteria infected cells acting as “Trojan horses” not only protect bacteria from antibiotic therapies and immune clearance, but also increase the dissemination of pathogens from the initial sites of infection. Antibiotics are hard and insufficient to treat such hidden internalized bacteria, especially multidrug-resistant (MDR) bacteria. Herein, aggregation-induced emission luminogens (AIEgens) such as N , N -diphenyl-4-(7-(pyridin-4-yl) benzo [ c ] [1,2,5] thiadiazol-4-yl) aniline functionalized with 1-bromoethane (TBP-1) and (3-bromopropyl) trimethylammonium bromide (TBP-2) (TBPs) show potent broad-spectrum bactericidal activity against both extracellular and internalized Gram-positive pathogens. TBPs trigger reactive oxygen species (ROS)-mediated membrane damage to kill bacteria, regardless of light irradiation. TBPs effectively kill bacteria without the development of resistance. Additionally, such AIEgens activate mitochondria dependent autophagy to eliminate internalized bacteria in host cells. Compared to the routinely used vancomycin in clinic, TBPs demonstrate comparable efficacy against methicillin-resistant Staphylococcus aureus (MRSA) in vivo. The studies suggest that AIEgens are promising new agents for the treatment of MDR bacteria associated infections.
研究领域:
药学
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微生物学
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材料科学
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产品关联
DOI
PubMed
高影响因子
GIANT
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2022
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IF 0
Yongmei Jia,Qiuxia Lai,Zhiguo Li,Guohua Zhou,Peilian Liu,Xunbo Lu,Mingyue Xue,Yong Cheng
Sensitive and accurate determination of aflatoxin B1 in food samples is urgently required for food safety, environment monitoring and human health due to its high toxicity. Here, we constructed an aptasensor with two fluorescent signals for determination of AFB1 in food samples by combining AIEgesns (TPE-Z) as fluorescent probe, the highly specific aptamer molecular label with Cy5 as the recognition element and GO as the fluorescence quencher. Upon the addition of AFB1, the fluorescence signal of the aptasensor platform turn from “off” to “on” with two fluorescent signals. The aptasensor provided a sensitivity (0.04 ng/mL), specificity and reliable approach for AFB1 detection. It had distinct advantage in detection sensitivity compared with single fluorescent signal aptasensor. Moreover, it was applied to analysis AFB1 in food samples (rice, corn and soybean) with satisfactory results.
研究领域:
食品安全
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分析化学
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分子生物学
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产品关联
DOI
ACS Omega
·
2024
·
IF 3.7
Yize Lin,Yan Tan,Rong Deng,Li Gong,Xiaoshan Feng,Zhongqi Cai,Yanxian He,Longbao Feng,Biao Cheng,Yi Chen
Chronic wounds are characterized with excessive biofluid and persistent infection. Therefore, there is an urgent desire to develop a multifunctional wound dressing that can meet the extreme requirements including effective antibacterial and powerful wound microenvironment regulation and protection function to promote wounds heal quickly. In this study, a multifunctional composite dressing (HA-AMP/SF/Alg/Rb-BG-AIEgens) was synthesized by combining a mesoporous bioactive glass framework loaded with AIEgens (Rb-BG-AIEgens) with cross-linked antimicrobial peptide grafted hyaluronic acid (HA-AMP), sodium alginate (Alg), and silk fibroin (SF). It is important to note that the Rb-BG-AIEgens can achieve real-time and sensitive bacterial detection. HA-AMP can achieve broad spectrum antibacterial and avoid the residue of drug-resistant bacteria. The HA-AMP/SF/Alg/Rb-BG-AIEgens dressing can up-regulate related proliferative proteins, thereby promoting regeneration of tissue and the rapid healing of chronic wounds. With good biocompatibility and antibacterial ability, HA-AMP/SF/Alg/Rb-BG-AIEgens dressing has great potential to become a next generation wound dressing for clinical biological fluid management and chronic bacterial infection treatment.
研究领域:
生物医学工程
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材料科学
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伤口愈合
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感染控制
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产品关联
DOI
PubMed
高影响因子
TETRAHEDRON
·
2022
·
IF 2.388
Yanan Gao,Xinwen Zhang,Jie Song,Liwei Dou,Hong Gao,Shibin Shang,Zhanqian Song
Dehydroabietic acid triarylamine (DTPA) synthesized from renewable natural rosin is an excellent hole-transporting material but is affected by aggregation-caused quenching in the solid state. Herein, two new biomass-based AIE luminogens (AIEgens), namely DTPA-TPE and 2DTPA-TPE, were synthesized through the attachment of DTPA to tetraphenylethene (TPE). Their aggregates in poor solvents and thin films have a strong emission with a fluorescence quantum yield of up to 67.4%. DTPA AIEgens exhibit superior thermal and morphological stability, showing high thermal degradation ( T 5d up to 412.0 °C) and glass transition ( T g up to 150.8 °C) temperatures. Electroluminescence (EL) devices with compounds used either as emitting layers (EMLs) or as EMLs and hole-transporting layers (HTLs) were constructed. The device with 2DTPA-TPE as the EML exhibits the best performance (turn-on voltage of 2.4 V, maximum luminance of 12,414 cd m −2 , current efficiency of 6.2 cd A −1 , and power efficiency of 6.5 lm W −1 ). The devices without the HTL ( N , N ′-di-1-naphthyl- N , N ′-diphenylbenzidine (NPB)) exhibit performances comparable with or better than those of the devices with NPB. These results indicate that these compounds are promising luminescent materials for non-doped organic light-emitting diodes.
研究领域:
有机化学
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材料科学
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光学工程
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产品关联
DOI
高影响因子
Polymer Chemistry
·
2018
·Vol. 9
·期 30
·4150-4160
·
IF 4.927
Qian Li,Xiao Li,Zhongying Wu,Yuhao Sun,Jianglin Fang,Dongzhong Chen
Tetraphenylethylene (TPE) is one of the well-known typical aggregation-induced emission (AIE) luminogens (AIEgens) and TPE-containing polymers possess excellent processing properties which facilitates the fabrication of various AIE systems and devices. For AIE active side-chain polymers, increasing the fluorescence quantum yield through suitable molecular design is very appealing. In this work, we present the well-controlled synthesis of a series of TPE polyacrylate side-chain polymers of Pm with short spacers with methylene number m = 0–5 via RAFT polymerization. All the obtained polymers exhibit typical and strong AIE characteristics in THF/H2O solution mixtures and in solid state films. In particular, greatly enhanced fluorescence emission is achieved with reduced spacer lengths, and further significant improvement achieved through annealing, which is ascribed to the restriction of intramolecular motions (RIM) and the blocking of the non-radiative channels due to overcrowding and confinement thanks to the strong positive coupling effect and the jacketing effect of side-chain polymers with very short spacers. The quantum yields of 34.4%, 32.0% and 31.4%, respectively, for the polymer films P0, P1 and P2 are several times higher than those of homologous polymers with longer spacers reported in the literature. Based on these TPE side-chain polymers of very short spacers, a simple, economic and visual method for detection of the explosive 2,4,6-trinitrotoluene (TNT) is demonstrated. Therefore, a facile approach and feasible strategy of simply adopting very short spacers for side-chain AIE active polymer preparation to significantly enhance the luminescence efficiency has been properly established and proposed, which may provide guidance and have a wealth of implications for the rational design and preparation of highly efficient luminescent AIE polymer materials.
研究领域:
材料科学
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高分子化学
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分析化学
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纳米技术
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产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2023
·Vol. 12
·期 12
·2202907
·
IF 10
Xinghua Yu,Ming Lyu,Xupei Ou,Wenquan Liu,Xing Yang,Xiaoxi Ma,Tianfu Zhang,Longnan Wang,Ying-Chuan Zhang,Sijie Chen,Ryan T. K. Kwok,Zheng Zheng,Hong-Liang Cui,Lintao Cai,Pengfei Zhang,Ben Zhong Tang
Aggregation-induced emission luminogens (AIEgens) are widely used as photosensitizers for image-guided photodynamic therapy (PDT). Due to the limited penetration depth of light in biological tissues, the treatments of deep-seated tumors by visible-light-sensitized aggregation-induced emission (AIE) photosensitizers are severely hampered. Microwave dynamic therapy attracts much attention because microwave irradiation can penetrate very deep tissues and sensitize the photosensitizers to generate reactive oxygen species (ROS). In this work, a mitochondrial-targeting AIEgen (DCPy) is integrated with living mitochondria to form a bioactive AIE nanohybrid. This nanohybrid can not only generate ROS under microwave irradiation to induce apoptosis of deep-seated cancer cells but also reprogram the metabolism pathway of cancer cells through retrieving oxidative phosphorylation (OXPHOS) instead of glycolysis to enhance the efficiency of microwave dynamic therapy. This work demonstrates an effective strategy to integrate synthetic AIEgens and natural living organelles, which would inspire more researchers to develop advanced bioactive nanohybrids for cancer synergistic therapy.
研究领域:
癌症治疗
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纳米技术
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生物物理学
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细胞生物学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2020
·
IF 7.1
Fei Zhang,Yaoming Liu,Binsheng Yang,Guangming Wen,Bin Liu
To recognize the lipid droplets (LDs) on the surface of trachea and realize the visualization of trachea, three TPA-based red/NIR fluorophores with high electron donor-acceptor (D-A) strength were designed and synthesized. These molecules showed donor-donor-π-acceptor (D 1 -D 2 -π-A) structure with the character of twisted intramolecular charge transfer (TICT). The photophysical experiment displayed that they had aggregation-induced emission (AIE) performance with red/NIR fluorescence. These AIEgens showed high sensitivity to LDs in living PC12 cells and excised corpus adiposum of Locusta migratoria . The visualization of the trachea is realized due to the large number of LDs distributed on the surface. Also, such compounds can effectively produce reactive oxygen species (ROS) with the irradiation of white light, demonstrating the potential photodynamic therapy (PDT) application on cancer cells. Moreover, the simulated experiment of ROS transferring from LDs simulator (trioctanoin) to cytoplasmic matrix (PBS) was carried out.
研究领域:
化学
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生物学
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材料科学
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光疗
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昆虫生理学
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2021
·Vol. 9
·期 15
·3317-3325
·
IF 6.331
Lixiu Chen,Bin Xia,Bing Yan,Jianhua Liu,Zhaohua Miao,Yan Ma,Jinchen Wang,Hu Peng,Tao He,Zhengbao Zha
Multifunctional contrast-enhanced agents suitable for application in surgical navigation by taking advantage of the merits of their diverse imaging modalities at different surgical stages are highly sought-after. Herein, an amphipathic polymer composed of aggregation-induced emission fluorogens (AIEgens) and Gd3+ chelates was successfully synthesized and assembled into ultrasound responsive microbubbles (AIE-Gd MBs) to realize potential tri-modal contrast-enhanced ultrasound (US) imaging, magnetic resonance imaging (MRI), and AIEgen-based fluorescence imaging (FI) during the perioperative period. Through ultrasound targeted microbubble destruction (UTMD) and cavitation effect, the as-prepared AIE-Gd MBs went through a MBs-to-nanoparticles (NPs) conversion, which not only resulted in targeted accumulation in tumor tissues but also led to stronger fluorescence being exhibited due to the more aggregated AIE-Gd molecules in the NPs. As a proof-of-concept, our work proposes a strategy of US-lit-up AIEgens in tumors which could offer a simple and powerful tool for surgical navigation in the future.
研究领域:
医学影像学
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生物材料学
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肿瘤学
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产品关联
DOI
PubMed
高影响因子
Small
·
2024
·
IF 13
Jun Zhu,Leyuan Tian,Dingyuan Yan,Dong Wang,Ben Zhong Tang
Exploiting single molecular species synchronously affording powerful second near-infrared (NIR-II) fluorescence, superior photoacoustic output, prominent reactive oxygen species generation, and satisfactory photothermal conversion is supremely appealing for phototheranostics, yet remains formidably challenging. In this work, electron donor/π-bridge engineering is implemented on the basis of 6,7-di(thiophen-2-yl)-[1,2,5]thiadiazolo[3,4- g ]quinoxaline moiety. The optimal molecule, namely TPATO-TTQ, is demonstrates to exhibit those notable features requested by exceptional phototheranostics, which are systematically elucidated through the depictions of excited-state energy dissipation pathways and the influence of intramolecular motion on the photophysical properties, with assistances of quantum chemical calculation and molecular dynamic simulation. By utilizing TPATO-TTQ nanoparticles, unprecedented performance on NIR-II fluorescence-photoacoustic-photothermal trimodal imaging-navigated type I photodynamic-photothermal synergistic therapy to orthotopic breast cancer is authenticates by the precise tumor diagnosis and complete tumor ablation.
研究领域:
生物医学工程
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肿瘤学
·
光学成像
✓ 已验证
DOI
PubMed
高影响因子
Small
·
2022
·Vol. 18
·期 24
·2201117
·
IF 15.153
Shikun Zhao,Jiangman Sun,Zhen Qin,Yufeng Li,Hao Yu,Guan Wang,Xinggui Gu,Kai Pan
White-light emitting elastomers (WLEEs) based on stimuli-responsive aggregation-induced emission (AIE) and regulated Förster resonance energy transfer (FRET) have aroused increasing attention due to the demands for wearable optoelectronic devices. Herein, the blue and orange AIEgens with different environmental sensitivities are synthesized and then encapsulated on both sides of nanofibers via side-by-side electrospinning aiming to achieve the Janus WLEEs. After regulating the blue-orange AIEgens ratio, efficient and stable white light emission with a CIE coordinate of about (0.33, 0.31) is achieved at a blue-orange AIEgens mass ratio of 3:1. Besides, the Janus nanofibers (Janus-NFs) also present super stretchability with elongation at the break over 150% and tensile strength close to 7 MPa. The sensitivity of fluorescence for Janus-NFs to its stretching deformation is used to visualize the evolution of the microstructure of nanofibers during stretching. Moreover, the Janus-NFs are also sensitive to HCl and NH 3 , of which the fluorescence color would change under HCl and NH 3 fuming above 2 and 57 ppm in air, respectively. The promising applications of the white light Janus-NFs in smart fabrics, warning sensors, and anti-counterfeiting packaging are demonstrated. This finding provides an efficient strategy for achieving wearable WLEEs with multiple functionalities, promoting the development of wearable devices.
研究领域:
材料科学
·
光学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2019
·Vol. 9
·期 2
·154
·
IF 4.034
Guifang Wang,Jing Wang,Linlin Zhao,Qiang Zhang,Yan Lu
In the present work, we describe a facile and general method of fabricating fluorescent inorganic nanoparticles with diverse shapes for cell imaging application. The hematite (α-Fe2O3) nanoparticles (HNPs) with three different shapes (i.e., spindle shape, ellipsoidal shape and quasi-spherical shape) were first prepared as model systems in consideration of good biocompatibility and the controllable morphology of α-Fe2O3. Three fluorescent HNPs with different shapes were readily achieved via one-pot sol-gel reaction of AIE luminogen-functionalized siloxane (AIEgen-Si(OCH3)3) and TEOS in the presence of PVP-stabilized HNPs. Due to the fluorescence originating from the thin AIEgens-contained SiO2shell around the HNPs, their photoluminescent intensities can be tuned by changing the concentrations of TEOS and AIEgen-Si(OCH3)3in feed prior to the sol-gel reaction. When the as-prepared fluorescent products were dispersed in water, they gave intense green light emission upon excitation at 360 nm with relatively high fluorescence quantum yield. Further, fluorescent HNPs exhibited low cytotoxicity and excellent photostability and, thus, were used as optical probes to preliminarily explore the effect of nanoparticle shapes on their cellular uptake behaviors. This work should open a facile way to prepare various fluorescent inorganic nanoparticles with specific morphology for various biological applications.
研究领域:
材料科学
·
生物医学工程
·
细胞生物学
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DOI
高影响因子
危险品化学品经营许可证（带存储）