TALANTA
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2017
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IF 4.162
Yaqi Mei,Qiong Hu,Baojing Zhou,Yonghui Zhang,Minhui He,Ting Xu,Feng Li,Jinming Kong
Simple and fast detection of alkaline phosphatase (ALP) activity is of great importance for diagnostic and analytical applications. In this work, we report a turn-off approach for the real-time detection of ALP activity on the basis of the charge transfer induced fluorescence quenching of the Cu(BCDS) 2 2- (BCDS = bathocuproine disulfonate) probe. Initially, ALP can enzymatically hydrolyze the substrate ascorbic acid 2-phosphate to release ascorbic acid (AA). Subsequently, the AA-mediated reduction of the Cu(BCDS) 2 2- probe, which displays an intense photoluminescence band at the wavelength of 402 nm, leads to the static quenching of fluorescence of the probe as a result of charge transfer. The underlying mechanism of the fluorescence quenching was demonstrated by quantum mechanical calculations. The Cu(BCDS) 2 2- probe features a large Stokes shift (86 nm) and is highly immune to photo bleaching. In addition, this approach is free of elaborately designed fluorescent probes and allows the detection of ALP activity in a real-time manner. Under optimal conditions, it provides a fast and sensitive detection of ALP activity within the dynamic range of 0–220 mU mL −1 , with a detection limit down to 0.27 mU mL −1 . Results demonstrate that it is highly selective, and applicable to the screening of ALP inhibitors in drug discovery. More importantly, it shows a good analytical performance for the direct detection of the endogenous ALP levels of undiluted human serum and even whole blood samples. Therefore, the proposed charge transfer based approach has great potential in diagnostic and analytical applications.
研究领域:
分析化学
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生物化学
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分子诊断
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药物发现
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 18
·2843-2850
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IF 4.776
Shi Gang Liu,Lei Han,Na Li,Na Xiao,Yan Jun Ju,Nian Bing Li,Hong Qun Luo
Nanozymes are increasingly exploited as components in bioanalysis and diagnostics. Here, we report a fluorescence and colorimetric dual-mode assay for alkaline phosphatase (ALP) activity employing oxidase-like cobalt oxyhydroxide (CoOOH) nanoflakes. Colorless o-phenylenediamine (OPD), a substrate for oxidase, can be oxidized to a product (OxOPD) with yellow color and orange fluorescence in a CoOOH nanoflake solution. But, ascorbic acid (AA) is able to reduce CoOOH to cobalt ion (Co2+), which causes the decomposition and collapse of the CoOOH nanoflakes, and thereby the CoOOH nanoflakes are deprived of the oxidase-like property. Based on this principle, the fluorescence and colorimetric dual-mode detection of AA was achieved. Alkaline phosphatase (ALP) can make L-ascorbic acid-2-phosphate (AAP) hydrolyze to yield AA. As a result, with the help of AAP, the selective and sensitive dual-mode assay of ALP activity has been realized successfully by using fluorescence and UV-vis absorption spectroscopies. Quantitative analysis of ALP in human serum samples and an ALP inhibitor investigation were performed using this sensing system. Given the economical and sensitive properties, the proposed method based on CoOOH nanoflakes has great potential for not only probing ALP activity in biological systems but also screening potential ALP inhibitors. Meanwhile, in this study, a new insight has been provided into the application of CoOOH nanoflakes in the development of sensitive and convenient sensors.
研究领域:
生物化学
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纳米技术
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分析化学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2016
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IF 4.712
Qiong Hu,Baojing Zhou,Pengyun Dang,Lianzhi Li,Jinming Kong,Xueji Zhang
We report a versatile approach for the colorimetric assay of alkaline phosphatase (ALP) activity based on the distinctive metal-to-ligand charge-transfer (MLCT) absorption properties of Fe(II)-phenanthroline reporter. In the presence of ALP, the applied substrate ascorbic acid 2-phosphate is enzymatically hydrolyzed to produce ascorbic acid, which then reduces Fe 3+ to Fe 2+ . The complexation of Fe 2+ with the bathophenanthroline disulfonate (BPS) ligand generates a blood-red Fe(BPS) 3 4− reporter, which is characterized by an intense MLCT absorption band at 535 nm in the visible range. Under optimal conditions, the spectral output exhibits a good quantitative relationship with ALP activity over the range of 0–220 mU mL −1 with a detection limit of 0.94 mU mL −1 . Moreover, the activity of ALP can also be conveniently judged through naked-eye observations. Results indicate that it is highly selective and can be applied to the screening of ALP inhibitors. In addition, it has been successfully employed to detect the endogenous ALP level of undiluted human serum samples, with a detection limit of 1.05 mU mL −1 being achieved. This approach avoids any elaborately designed substrates and holds considerable simplicity and flexibility for reporter design. This study broadens the horizon of the applications of phenanthroline-based transition metal complexes. Furthermore, an efficient and practical method like this has the potential to be widely used in clinical applications and in the point-of-care testing.
研究领域:
分析化学
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生物化学
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临床诊断
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 27
·14995-15000
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IF 2.936
Wenjuan Zhang,Hanxiao Yang,Nan Li,Na Zhao
In this work, we report a fluorescent probe (TPEQN-P) for detecting alkaline phosphatase (ALP) with high sensitivity and monitoring its activity based on the specific aggregation-induced emission (AIE) effect. TPEQN-P can be constructed by conjugating the phosphate moiety into an AIE molecule (TPE-QI) through a reactive p-hydroxybenzyl group, which exhibits very weak emission in aqueous media due to its good water solubility. In the presence of ALP, TPEQN-P undergoes dephosphorylation and releases the hydrolysis product TPE-QI, which is intensely fluorescent because of its poor water solubility. The detection limit for ALP using TPEQN-P can be as low as 0.0077 U L−1 with a linear range of 0–30 mU mL−1 in solution. TPEQN-P also shows excellent applicability in serum samples, demonstrating potential applications in clinical diagnosis and biomedical research. TPEQN-P also can be applied for the determination of ALP activity and in ALP inhibitor screening.
研究领域:
分析化学
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生物化学
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分子生物学
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临床化学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 37
·6008-6015
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IF 4.776
Fengyi Wang,Xuan Hu,Jing Hu,Qianqian Peng,Baozhan Zheng,Juan Du,Dan Xiao
In this study, bimetallic lanthanide coordination polymer nanoparticles (Tb-GMP-Eu CPNs) were developed for sensing alkaline phosphatase (ALP) by utilizing guanine monophosphate (GMP) as the bridge ligand to self-assemble with Tb3+ and Eu3+ in Tris–HCl buffer solution. In this polymer framework, an efficient energy transfer from Tb3+ to Eu3+ occurred because the emission energy of Tb3+ matched well with the absorption energy of Eu3+. As a result, the synthesized Tb-GMP-Eu CPNs exhibited the characteristic red emission of Eu3+ when excited at 290 nm. When ALP was introduced into the Tb-GMP-Eu CPNs system, it would destruct the combination between Tb and GMP by a dephosphorylation process, resulting in interruption of energy transfer from Tb3+ to Eu3+ and therefore the fluorescence quenching of Eu3+. Based on these facts, a fluorescence assay based on bimetallic lanthanide coordination polymer nanoparticles for ALP detection was developed. Such a Tb-GMP-Eu CPNs sensor exhibited sensitive response to ALP with a detection limit down to 0.004 U L−1. Moreover, the successful application of ALP inhibitor evaluation and determination of ALP in serum samples have been demonstrated. Therefore, the proposed method has great potential application for ALP detection in biological and clinical diagnosis.
研究领域:
分析化学
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生物化学
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纳米技术
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生物医学工程
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临床诊断
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2021
·Vol. 9
·期 13
·2998-3004
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IF 6.331
Jia An,Yongqin Hu,Guoyi Liu,Meizhu Chen,Rubing Chen,Ying Lyu,Mengdi Yuan,Mengfei Luo,Yufei Liu
Considering the limited sensitivity and accuracy of single-signal assay strategies, the multi-signal assay strategy has sparked significant excitement in recent years. In this study, for the first time, we reported a one-pot method in situ synthesis of carbon-containing nanoparticles (CNPs) via p-aminophenol (AP) and diethylenetriamine (DETA). The CNP solution exhibits yellow and light blue fluorescence under UV-light. Moreover, the CNPs exhibited excellent photoluminescence stability even under extreme conditions. Inspired by the alkaline phosphatase (ALP)-triggered specific catalytic reaction, we constructed an ultrasensitive fluorescence and colorimetric two-channel strategy for monitoring the ALP activity. By optimizing the detection parameters, the detection limits for both fluorometric and colorimetric were 0.05 mU mL−1. Moreover, the strategy showed high specificity and was successfully applied to monitor the ALP activity level in human serum samples. The analytical strategy opened a new window for the detection of the ALP activity, screening of the ALP inhibitor, and disease diagnosis.
研究领域:
分析化学
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生物传感器
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纳米技术
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医学诊断
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产品关联
DOI
PubMed
高影响因子
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