WEED SCIENCE
·
2025
·
IF 2.5
Shaojing Yin,Guangtao Zhu,Yiheng Li,Liyao Dong,Zhike Feng
Italian ryegrass [Lolium perenne L. ssp. multiflorum (Lam.) Husnot], a pernicious weed in wheat fields, has evolved severe resistance to the acetolactate synthase (ALS)-inhibiting herbicides, like pyroxsulam. Here, the derived cleaved amplified polymorphic sequence (dCAPS) markers were developed to detect two distinct mutations at the 197th position of the ALS gene. The method was used to examine and purify the resistant population. Homozygous populations with different 197 site mutations from the same population were obtained, and the target-site resistance mechanisms were investigated. Whole-plant dose-response bioassays show that the resistance index (RI) of the Pro-197-Thr mutant population to pyroxsulam was 508.92, whereas that of the Pro-197-Gln population was 9.75. Similar trends were observed for different herbicides within same mode of action. In vitro ALS assays demonstrated that the Pro-197-Thr population exhibited lower sensitivity to pyroxsulam than the Pro-197-Gln population, consistent with plant bioassays. Furthermore, ALS gene expression of the Pro-197-Thr population analysis is significantly higher than that in the Pro-197-Gln population which may also explain why the Pro-197-Thr population exhibits higher resistance level than the Pro-197-Gln population. Our findings suggest that different amino acid substitutions at one ALS gene locus can confer herbicide resistance with different levels in L. perenne ssp. multiflorum. This study provides valuable insights into the mechanisms of herbicide resistance in L. perenne ssp. multiflorum.
研究领域:
植物学
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植物科学
·
杂草科学
·
分子生物学
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遗传学
·
农学
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DOI
高影响因子
JOURNAL OF NEUROSCIENCE
·
2026
·
IF 4.3
Xiaoli Su,Xingli Tan,Ying Wang,Weiwei Liang,Di Wang,Di Huo,Hongyong Wang,Yan Qi,Wenmo Zhang,Ling Han,Dongmei Zhang,Ming Wang,Jing Xu,Shuyu Wang,Jing Wang,Honglin Feng
Mutations in superoxide dismutase 1 (SOD1) compromise its metal-binding capacity, resulting in protein misfolding and aggregation, which ultimately induces cellular apoptosis in amyotrophic lateral sclerosis (ALS). Copper metabolism domain containing 1 (COMMD1), a gene implicated in copper homeostasis, has not been thoroughly characterized in the context of ALS pathogenesis. In this study, we identified elevated COMMD1 expression in ALS, potentially contributing to diminished copper incorporation into SOD1. Knockdown of COMMD1 enhanced palmitoylation of the copper chaperone for SOD1 (CCS), facilitating its membrane translocation and promoting copper loading into SOD1, thereby conferring neuroprotection in ALS. Mechanistically, we established that COMMD1 knockdown augments CCS palmitoylation via activation of the hypoxia inducible factor 1 subunit alpha (HIF-1α)/fatty acid synthase (FASN) signaling axis. In vivo investigations utilizing male hSOD1G93A transgenic mice demonstrated that COMMD1 deficiency markedly ameliorated the deterioration of motor function and prolonged survival duration. These findings collectively suggest that COMMD1 represents a potential therapeutic target for ALS intervention.Significance Statement Superoxide dismutase 1 (SOD1) was the first identified mutant gene associated with amyotrophic lateral sclerosis (ALS). Mutations in SOD1 compromise its metal-binding function, resulting in neuronal apoptosis, a hallmark of ALS pathogenesis. Utilizing the SOD1G93A models of ALS, our findings revealed that COMMD1 deficiency significantly elevates copper incorporation into SOD1, consequently attenuating cellular apoptosis. These results suggest that targeted inhibition of COMMD1 could represent a potential therapeutic strategy for ALS treatment.
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2024
·Vol. 48
·期 42
·18166-18176
·
IF 2.7
Jing Xiao,Linshan Peng,Ailan Ouyang,Xin Mo,Yiping Yao,Chunyan Wang,Chunpei Yan
A phosphonate-functionalized Zr@FMA material was prepared in this work as an adsorbent for the efficient recovery and enrichment of Th(IV) from aqueous solutions. The adsorbent, prepared by functionalizing Zr@FMA with alendronate sodium (ALS), a well-known active bisphosphonate, demonstrates good water stability and a large surface area, making it suitable for the separation and enrichment of Th(IV) in aqueous solutions. A comparison of the adsorption behavior of Th(IV) on Zr@FMA-ALS with the unfunctionalized Zr@FMA reveals that the introduction of phosphonate groups significantly enhances the maximum adsorption capacity and rate of Zr@FMA-ALS. The adsorption data aligns well with the Langmuir isotherm model, suggesting a uniform distribution of Th(IV) on the adsorbent surface in a monolayer coverage. Thermodynamic analysis indicates an endothermic and spontaneous adsorption process. Zr@FMA-ALS also shows excellent selectivity for Th(IV) over other metal ions in mixed solutions. Insights into the adsorption mechanism are provided through SEM, XPS, and FT-IR analyses, indicating that ALS molecules play a crucial role in capturing Th(IV) on the surface of Zr@FMA-ALS.
研究领域:
化学工程
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材料科学
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环境科学
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产品关联
DOI
高影响因子
Chemical & Biomedical Imaging
·
2023
·
IF 0
Yan Zhang,Ning Xu,Chunyu Yan,Xuelian Zhou,Qinglong Qiao,Lu Miao,Zhaochao Xu
The aggregation of fusion in sarcoma (FUS) in the cytoplasm and nucleus is a pathological feature of Amyotrophic lateral sclerosis (ALS) and Frontotemporal Dementia (FTD). Genetic mutations, abnormal protein synthesis, environmental stress, and aging have all been implicated as causative factors in this process. Salt ions are essential to many physiological processes in the body, and the imbalance of them is an important environmental stress factor in cells. However, their effect on liquid–liquid phase separation (LLPS) of FUS proteins in living cells is not well understood. Here, we map the various salt-induced LLPS of FUS in living cells by genetically coding and self-labeling FUS with organic dyes. The brightness and photostability of the dyes enable long-term imaging to track the mechanism of the assembly and disappearance of FUS phase separation. The FUS protein showed a better phase separation tendency under 0.3 M salt stimulation, and there was a large amount of FUS shuttling from the nucleus to the cytoplasm. At this concentration, various salt solutions displayed different effects on the phase separation of FUS protein, following the Hofmeister effects. We further observed that the assembly of FUS droplets underwent a process of rapid formation of small droplets, plateaus, and mutual fusion. Strikingly, The CsCl-stimulated FUS droplets were not completely reversible after washing, and some solid-like granules remained in the nucleus. Taken together, these results help broaden our understanding of the LLPS of FUS proteins in cellular stress responses.
研究领域:
神经退行性疾病
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细胞生物学
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生物化学
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分子生物学
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DOI
Scientific Reports
·
2019
·Vol. 9
·期 1
·1-9
·
IF 4.011
Shao Yongni,Gu Weimin,Jiang Linjun,Zhu Yiming,Gong Aiping
As an ideal raw material for the production of astaxanthin, H . pluvialis was drawing attention during the last few years, there are some research topics initiated to find out the synthetic pathway of astaxanthin in H . pluvialis . In this study, confocal microscopic Raman technology was utilized to analyze the point-by-point mapping for H . pluvialis , and the visualization of pigment such as carotenoid and astaxanthin content were achieved. By comparing the Raman spectra of H . pluvialis and standard product of astaxanthin, and using the C = C stretching vibration of the Raman intensity as the main indicator for carotenoids, the visual spatial distribution for the carotenoids content was obtained. The MCR-ALS was applied to analyze the Raman data of H . pluvialis , and the information of astaxanthin was extracted to achieve real-time spatial distribution. The visualization of astaxanthin content shows that MCR-ALS is very effective for extracting the information of astaxanthin content from H . pluvialis . By exploring the spatial distribution of carotenoids and astaxanthin contents, analyzing the spatial distribution rules during its growth, Raman spectroscopy technology can be utilized to investigate the internal components of the pigment (ataxanthin, etc.) in H . pluvialis , which make it as an effective methodology to monitor the accumulation and changing mechanism of pigment content in microalgae.
研究领域:
生物化学
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光谱学
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藻类研究
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DOI
PubMed
高影响因子
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
·
2026
·Vol. 123
·期 26
·e2610001123
·
IF 9.5
Dongyue Liu,Yu Li,Shuai Huang,Yufang Xu,Lei Sun,Wen Li,Cahir J. O’Kane,David C. Rubinsztein,Bingwei Lu,Shuangxi Li
Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) are progressive neurodegenerative disorders characterized by motor neuron degeneration, leading to muscle weakness, atrophy, and cognitive impairments. A defining pathological hallmark of ALS/FTD is the cytosolic mislocalization and accumulation of TAR DNA-binding protein 43 (TDP-43), highlighting its critical role in ALS pathogenesis. However, the molecular mechanisms underlying TDP-43 proteostasis remain poorly understood. Through a genetic screening approach, we identify inositol-requiring enzyme 1 (IRE1), an endoplasmic reticulum-resident transmembrane protein, as a potent suppressor of TDP-43 protein levels. Furthermore, we show that ribosome-associated quality control (RQC) factors play a crucial role in regulating TDP-43 proteostasis and cellular toxicity. Activation of the RQC pathway prevents excessive accumulation of TDP-43 and associated toxicity. Mechanistically, our findings suggest that IRE1 regulates TDP-43 protein level by promoting the degradation of aberrant TDP-43 translation product through the RQC pathway. IRE1 acts canonically to enhance the transcription of the RQC core component Clbn/NEMF and noncanonically to physically interact with Clbn/NEMF, thereby ameliorating TDP-43-induced proteotoxicity. Moreover, ectopic expression or pharmacological activation of IRE1 alleviates TDP-43 pathology and restores cognitive function in the TDP-43 A315T ALS mouse models. Collectively, our study identifies a role for IRE1 in the translational quality control of TDP-43 and establishes its potential as a therapeutic target for ALS/FTD.
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DOI
高影响因子
BRAIN RESEARCH
·
2025
·
IF 2.6
Xingli Tan,Xiaoli Su,Ying Wang,Weiwei Liang,Di Wang,Di Huo,Hongyong Wang,Yan Qi,Wenmo Zhang,Ling Han,Dongmei Zhang,Ming Wang,Jing Xu,Shuyu Wang,Jing Wang,Honglin Feng
Dysregulation of copper and iron homeostasis contributes to the progression of amyotrophic lateral sclerosis (ALS), but the role and mechanism of COMM domain containing 4 (COMMD4) in ALS remains unclear. In this research, we showed that the expression of COMMD4 was upregulated in ALS cells and animal models. The increased COMMD4 induced neuronal ferroptosis by disrupting the Cu-Fe balance. Mechanistic studies indicated that COMMD4 inhibited ferroportin (FPN)-mediated neuronal iron efflux by inhibiting intracellular copper and hephaestin (HEPH). Our findings demonstrated that COMMD4 depletion exerts neuroprotective effects on ALS by increasing intracellular copper and activating HEPH/FPN pathway, rather than affecting the interaction between HEPH and FPN. Targeting COMMD4 and its downstream signaling pathways may offer potential therapeutic avenues for ALS.
研究领域:
神经生物学
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分子生物学
·
医学
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产品关联
DOI
PubMed
高影响因子
WEED SCIENCE
·
2024
·
IF 2.1
Yu Liu,Kerong Shi,Haitao Gao,Shaojing Yin,Liyao Dong,Zhike Feng
Barnyardgrass [Echinochloa crus-galli (L.) P. Beauv.] is increasingly infesting imidazolinone-tolerant (IMI-T) rice fields in China, imazamox resistance of E. crus-galli has become the major concern for weed management in IMI-T rice fields. In this study, the susceptible population JLGY-3 (S) and the suspected resistant population JHXY-2 (R) collected from IMI-T rice fields were used as research subjects. When treated with imazamox, JHXY-2 (R) population showed a high level of herbicide resistance with a resistance index (RI) of 31.2. JHXY-2 (R) was cross-resistant to all five acetolactate synthase (ALS) inhibitors from different chemical families, but sensitive to herbicides inhibiting acetyl-CoA carboxylase (ACCase). In order to understand the reason why JHXY-2 (R) was resistant to imazamox, we performed experiments to characterize potential TSR and NTSR mechanisms. A trp-574-leu amino acid mutation in ALS and low imazamox ALS sensitivity were the main mechanism underlying imazamox resistance in this JHXY-2 (R) population. There was no significant difference in ALS gene expression and ALS protein abundance between R and S populations. High-performance liquid chromatography-tandem mass spectrometry analysis showed enhanced metabolism of imazamox in JHXY-2 (R), which was in contrast to the results of pretreatment with a metabolic enzyme inhibitor. Treatments with the P450/GST inhibitors did not alter the resistance level of JHXY-2 (R) against imazamox. To further clarify the NTSR mechanism of JHXY-2 (R), transcriptome sequencing showed that there was almost no significant difference in the expression of P450 and GST metabolic enzyme genes between R and S populations, and only GST-U1 showed a significant induction in R population. In conclusion, amino acid mutation and higher enzyme activity of ALS are the main causes of imazamox resistance in JHXY-2 (R). However, given the differences in imazamox residues in the leaves of the E. crus-galli, there may still be undetectable NTSR that are causing imazamox resistance in the R population.
研究领域:
植物学
·
遗传学
·
农业科学
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产品关联
DOI
高影响因子
Plants-Basel
·
2021
·Vol. 10
·期 7
·1309
·
IF 3.935
Xiaoyue Yu,Hanwen Wu,Jianping Zhang,Yongjie Yang,Wei Tang,Yongliang Lu
Polypogon fugaxis a common winter weed in China and other Asia countries. We have previously found aP. fugaxbiotype (R) resistant to acetyl co-enzyme A carboxylase (ACCase) herbicides also cannot be effectively controlled by some acetolactate synthase (ALS) herbicides. This study evaluated the level of resistance to four ALS herbicides (metsulfuron-methyl, chlorsulfuron, monosulfuron, pyribambenz isopropyl) in the R biotype and the associated resistance mechanism. The R biotype exhibited moderate level of resistance to metsulfuron-methyl (6.0-fold) compared with the sensitive biotype (S). Sequence analysis ofALSgene revealed that twoALSgenes existed inP. fugax. However, no substitution associated with ALS resistance mechanism were found inALSgenes between the S and R biotypes. The activity of ALS enzyme isolated from the R biotype was inherently higher and less sensitive to metsulfuron-methyl than the S biotype. Glutathione S-transferases (GST) activity was also less sensitive to metsulfuron-methyl in the R than as the S biotypes. Malathion, a cytochrome P450 (CYP) monooxygenase inhibitor, had much greater synergistic effect with metsulfuron-methyl on the R than as the S plants, reducing the ED50value (herbicide dose to inhibit growth by 50%) of metsulfuron-methyl by 23- and 6-fold, respectively, suggesting that CYP mediated enhanced metabolism might contribute to the resistance to ALS herbicides. These results suggest that metsulfuron-methyl resistance in the R biotype was associated with the up-regulated ALS enzymatic activity and the GST and CYP-mediated enhanced herbicide metabolism.Keywords:metsulfuron-methyl;ALS enzyme activity;Polypogon fugax;glutathione S-transferases;cytochrome P450
研究领域:
植物学
·
分子生物学
·
农业科学
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DOI
PubMed
高影响因子
Chilean Journal of Agricultural Research
·
2017
·Vol. 77
·期 3
·257-265
·
IF 0.719
Wei Li,Lele Zhang,Ning Zhao,Wenlei Guo,Weitang Liu,Lingxu Li,Jinxin Wang
ABSTRACT American slough grass ( Beckmannia syzigachne (Steud.( Fernald) is a worldwide weed and is widely distributed in rice-wheat rotations in China. Fenoxaprop- P -ethyl and mesosulfuron-methyl are two major herbicides used to control B. syzigachne . Resistance has evolved in B. syzigachne under continuous selective pressure from herbicides. This study aimed to establish the cross-resistance pattern of a resistant population and explore the potential non-target-site based resistance mechanisms of B. syzigachne . Sequencing of target enzyme genes (acetyl coenzyme A carboxylase (ACCase( and acetolactate synthase (ALS() revealed that there were no resistance-endowing amino acid substitutions in the resistant B. syzigachne population (R F1 ) compared with the sensitive population (S F1 ), and obtained the purified materials. Furthermore, piperonyl butoxide (PBO) and malathion showed synergistic effects with fenoxaprop- P -ethyl and mesosulfuron-methyl respectively in R F1 . Therefore, we speculate that the resistance observed in B. syzigachne was related with metabolic, mostly involving the cytochrome P450 enzymes. Cross resistance patterns showed that the purified resistant B. syzigachne produce high resistance to fenoxaprop- P -ethyl and pyroxsulam; intermediate resistance to flucarbazone-sodium; low resistance to quizalofop- P -ethyl, clodinafop-propargyl, sethoxydim and mesosulfuron-methyl; sensitive to clethodim pinoxaden and isoproturon. Key words: Herbicide resistance; metabolic resistance; P450 inhibitors; target enzymes sequencing.
研究领域:
植物学
·
分子生物学
·
农业科学
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产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2018
·Vol. 75
·期 1
·204-214
·
IF 3.249
Ning Zhao,Yanyan Yan,Lu'an Ge,Baolin Zhu,Weitang Liu,Jinxin Wang
BACKGROUND Shortawn foxtail ( Alopecurus aequalis Sobol.) is a competitive grass weed infesting winter wheat- and canola-growing fields in China. In May 2016, a suspected A. aequalis resistant population AHTC-06 that survived fenoxaprop- P -ethyl and mesosulfuron-methyl applied at their field-recommended rates was collected from a wheat field in Jinji County, Anhui Province, China. This study aimed to determine the resistance profile of this AHTC-06 population to ACCase- and ALS-inhibitors, and to investigate its mechanisms of resistance to fenoxaprop- P -ethyl and mesosulfuron-methyl. RESULTS Two mutations, a common Ile-2041-Asn ( ACCase gene) and a very rare Pro-197-Tyr ( ALS1 gene), were both identified in resistant individual plants. The homozygous subpopulation AHTC-06F1 for the two mutations was generated, and it showed broad-spectrum resistance to APPs, DENs, and ALS-inhibiting herbicides of all five chemical families tested, with resistance index (RI) values that ranged from 2.2 to 36.5. In vitro ALS activity assays showed the ALS from the resistant population was insensitive to all the tested ALS inhibitors, with RI values ranging from 3.10 to 22.51. Pre-treatment with piperonyl butoxide (PBO) and malathion significantly ( P < 0.05) reversed the weed's resistance to fenoxaprop- P -ethyl and mesosulfuron-methyl, respectively. Two P450 genes, c21190_g1 and c43350_g3 , were constitutively overexpressed and mesosulfuron-methyl-induced upregulated in resistant plants, for which c43350_g3 was also fenoxaprop- P -ethyl-induced upregulated. CONCLUSION This study confirms the first case of a grass weed featuring broad-spectrum resistance to ALS-inhibiting herbicides due to a Pro-197-Tyr mutation in the ALS gene. Fenoxaprop- P -ethyl and mesosulfuron-methyl resistances in AHTC-06 plants were conferred by target site mutations and P450s-based metabolism. © 2018 Society of Chemical Industry
研究领域:
植物科学
·
杂草科学
·
分子生物学
·
遗传学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Shao Hua Lu,Sha Sha Li,Bo Yin,Jia Ying Mi,Hong Lin Zhai
Diverse interferences often affect the determination of pesticide residues in various kinds of food, and complex pretreatments are necessary. An effective approach for the simultaneous quantitative analysis of multi-target components in different substrates was proposed, and applied to the determination of ternary pesticides in cherry tomatoes and red grape samples. By means of HPLC-DAD, the spectra were obtained under isocratic elution. Utilizing the Tchebichef image moments, unified quantitative models were established for the three target components in the samples with two different substrates, respectively. The correlation coefficients of leave-one-out cross-validation ( R loo - c v 2 ) of the obtained models were more than 0.9975. The predictive correlation coefficients ( R p 2 ) were more than 0.9831. Compared with the N-PLS and MCR-ALS methods, the proposed approach is not only more accurate and reliable, but also can extract essential information and expected to be a potential tool to rapidly quantify multi-component in different substrates without complex pretreatments.
研究领域:
分析化学
·
食品科学
·
化学计量学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）