JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Yunhao Xie,Lun Lu,Baoliang Chen
The demands for genuine remediation of heavy metal contaminated soil have triggered extensive studies in the soil washing method. However, numerous soil washing methods show poor sustainability for target soil, due to the tremendous cost, hidden secondary pollution and severe soil deterioration. Here, an asymmetrical alternating current electrochemically-mediated remediation platform (ACRP) is developed by fabricating an amidoxime-functionalized electrode (Ami-electrode). The real soil contaminated with 1200 mg/kg Cr(VI) is remediated efficiently to less than safety level (30 mg/kg), meanwhile no exorbitant soil nutrient loss is observed and no secondary pollution occurs. Furthermore, the consumption of washing effluents for the ACRP method is 24 times lower than the traditional washing method. Ami-electrode with asymmetrical alternating current promote the electrocatalytic efficiency by inhibiting the Coulomb repulsion between Cr(VI) species and cathode. With the aid of Ami-electrode and positive bias, Cr(VI) species in effluents are adsorbed on chelating site. By subsequent negative bias, Cr element is reduced and recycled in the less hazardous form of amorphous Cr(III) hydroxide, and effluents are regenerate concurrently in a cyclic system. Durability experiment and cost calculation verify the exceptional sustainability and feasibility for remediation practices. This work provides a sustainable remediation method for Cr(VI)-contaminated soil, and then paves the way to develop electrochemically soil remediation platform for practical applications.
研究领域:
环境科学
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电化学
·
土壤修复技术
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DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Jie-Ping Fan,Ping Gan,Feng-Hui Tao,Hui-Ping Chen,Hai-Long Peng,Yu-De Liu,Chun-Fang Xie
Oleanolic acid possesses a wide variety of pharmacological activities and spreads in various plants, but always co-exist with its isomers ursolic acid. Separating oleanolic acid with high efficacy from ursolic acid contaminated plant extracts is quite important. This study prepared a molecularly imprinted poly(OA[AMI] supramolecular hydrogel) (MIPSHG@OA) for selectively enriching oleanolic acid, by using the 1-allyl-3-methylimidazolium oleanolic salt (OA[AMI]) as hydrogelator, template, functional monomer and basement. The preparation conditions were optimized as 12.5 mg OA[AMI], 40 mg MBA, 4 ml solvent, 10 mg APS, and 30 μl TEMED. The characterization by FT-IR spectra, TG analysis, SEM image and equilibrium swelling ratio showed a sheet and porous structure and a large equilibrium swelling ratio of the obtained MIPSHG@OA. The adsorption performance showed the obtained MIPSHG@OA was homogeneous on the surface and uniform on the adsorption sites, the adsorption of oleanolic acid on the polymers was chemisorption and monolayer, the adsorbed oleanolic acid molecules did not interact with each other, the MIPSHG@OA had high competitive recognition performance and imprinting efficiency on oleanolic acid, and had good recyclability. In the solid phase extraction application, the results demonstrated that the synthesized MIPSHG@OA could selectively enrich oleanolic acid.
研究领域:
分析化学
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药学
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植物化学
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产品关联
DOI
高影响因子
Science China-Chemistry
·
2021
·Vol. 64
·期 12
·2230-2238
·
IF 9.445
Liang Xu,Mi Lan,Yu Zhengyou,Wang Minghan,Hu Yuecong,Zheng Xinhe,Shao Yi,Zhu Zhiwei,Shao Yuanhua
Simplicity and stability of an analytical method are the key to practical applications. In order to achieve such a goal, a molecule with multifunction could be one of the strategies. In this work, the multifunctional characteristics of branched polyethylenemine (bPEI) were employed for verification of this hypothesis. Three types of amines in the bPEI can be used to synthesize core-shell nanostructures (bPEI@Au and SiO2@bPEI@Au) in a simple one-pot process within 1 h, and as coreactants with over 500-fold enhancement for electrochemiluminescent (ECL) detection. Based on the remarkable performance of bPEI, a label-free ECL immunosensor and its array with the same assembly strategy were constructed for the simple and rapid determination of three acute myocardial infarction (AMI) biomarkers: cardiac troponin I (cTnI), human-type fatty acid binding protein (hFABP) and copeptin. Both methods exhibited excellent stability and sensitivity with wide linear range and low limits of detection down to fg/mL level by the photomultiplier tube and pg/mL level by the electron-multiplying charge-coupled device.
研究领域:
分析化学
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纳米技术
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生物医学工程
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材料科学
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DOI
高影响因子
ANALYTICAL LETTERS
·
2024
·
IF 1.6
Yueting Ding,Yulan Song,Wei Xu,Qi Zhang,Yanwen Li,Qiangling Zhang,Qu Liang,Xun Bao,Dianlong Ge,Yan Lu,Lei Xia,Yawei Liu,Chaoqun Huang,Xue Zou,Chengyin Shen,Yannan Chu
Acetone is produced from fat metabolism which is increased in diabetic patients due to the disruption of glucose metabolism. Therefore, urinary acetone is expected to be a biomarker for noninvasive diagnosis of diabetes. Although several techniques have been developed for urinary acetone, none meet the clinical needs of automated multi-injection for numerous samples. In this study, we developed automated multi-injection gas chromatography-photoionization detection (AMI-GC-PID) to determine urinary acetone. First, we optimized the sample preprocessing during urine collection and storage before detection and established a standard protocol. The urine was immediately sealed in headspace bottles, stored at 20 °C for 1 h, and equilibrated for 20 min at 80 °C before analysis. Next we evaluated the repeatability of the method and the influence of the urine matrix. The relative standard deviations (RSDs) of the intra-day (nine measurements) and inter-day (three days) measurements were less than 5%. The recovery rate was 97.4% ± 4.6%. AMI-GC-PID was applied to determine urinary acetone in 44 diabetic patients and 29 healthy subjects. The median concentration of urinary acetone was much higher in diabetic patients than in healthy controls (2019 μg/L compared 699 μg/L). The results show that AMI-GC-PID possesses suitable analytical figures of merit for urinary acetone with broad applications in the noninvasive diagnosis of diabetes.
研究领域:
医学分析
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代谢组学
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临床化学
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DOI
高影响因子
PeerJ
·
2024
·
IF 2.3
Bingyu Wang,Jianqing Zhou,Ning An
Cuproptosis-related key genes play a significant role in the pathological processes of acute myocardial infarction (AMI). However, a complete understanding of the molecular mechanisms behind this participation remains elusive. This study was designed to identify genes and immune cells critical to AMI pathogenesis. Based on the GSE48060 dataset (31 AMI patients and 21 healthy persons, GPL570-55999), we identified genes associated with dysregulated cuproptosis and the activation of immune responses between normal subjects and patients with a first myocardial attack. Two molecular clusters associated with cuproptosis were defined in patients with AMI. Immune infiltration analysis showed that there was significant immunity heterogeneity among different clusters. Multiple immune responses were closely associated with Cluster2-specific differentially expressed genes (DEGs). The generalized linear model machine model presented the best discriminative performance with relatively lower residual and root mean square error, and a higher area under the curve (AUC = 0.870). A final two-gene-based generalized linear model was constructed, exhibiting satisfactory performance in two external validation datasets (AUC = 0.719, GSE66360 and AUC = 0.856, GSE123342). Column graph, calibration curve, and decision curve analyses also proved the accuracy of AMI prediction. We also constructed a mouse C57BL/6 model of AMI (3 h, 48 h, and 1 week) and used qRT-PCR and immunofluorescence to detect the expression changes of CBLB and ZNF302. In this study, we present a systematic analysis of the complex relationship between cuproptosis and a first AMI attack, and provide new insights into the diagnosis and treatment of AMI.
研究领域:
生物信息学
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医学
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心血管疾病
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DOI
PubMed
高影响因子
Heliyon
·
2024
·
IF 3.4
Shuo Li,Xiang-Yu Pei,Xin-Yi Liu,Shu-Liang Wang,Wen Xu,Jing-Jing Wang,Zhen Feng,Han Ding,Yin-Feng Zhang,Rui Zhang
MicroRNAs (miRNAs) are small noncoding RNAs of 18–25 bases. miRNAs are also important new biomarkers that can be used for disease diagnosis in the future. Studies have shown that miR-124 levels are significantly elevated during acute myocardial infarction (AMI) and play a key role in the cardiovascular system. A variety of methods have been established to detect myocardial infarction-related miRNAs. However, most require complex miRNA extraction and isolation, and these methods are virtually undetectable when RNA levels are low in the sample. It may lead to biased results. Thus, it is necessary to develop a technique that can detect miRNA without extracting it, which means that intracellular detection is of great significance. Here, we improved the traditional silicon spheres and obtained a biosensor that could effectively capture and detect specific noncoding nucleic acids through the layer-by-layer assembly method. The sensor is protected by hyaluronic acid so it can successfully escape the lysosome into the cell and achieve detection. With the help of a full-featured microplate reader, we determined that the detection limit of the biosensor could reach 1 fM, meeting the needs of intracellular detection. At the same time, we prepared an oxidative stress cardiomyocyte infarction model and successfully captured the overexpressed miR-124 in the infarcted cells to achieve in situ detection. This study could provide a new potential tool to develop miRNAs for sensitive diagnosis in AMI, and the proposed strategy implies its potential for biomedical research.
研究领域:
分子生物学
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生物医学工程
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心脏病学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-11
·
IF 14.7
Li Juanhua,Liu Yiren,Wu Tianyu,Xiao Zihan,Du Jianhang,Liang Hongrui,Zhou Cuiping,Zhou Jianhua
Acute myocardial infarction (AMI) has become a public health disease threatening public life safety due to its high mortality. The lateral-flow assay (LFA) of a typical cardiac biomarker, troponin I (cTnI), is essential for the timely warnings of AMI. However, it is a challenge to achieve an ultra-fast and highly-sensitive assay for cTnI (hs-cTnI) using current LFA, due to the limited performance of chromatographic membranes. Here, we propose a barbed arrow-like structure membrane (BAS Mem), which enables the unidirectional, fast flow and low-residual of liquid. The liquid is rectified through the forces generated by the sidewalls of the barbed arrow-like grooves. The rectification coefficient of liquid flow on BAS Mem is 14.5 (highest to date). Using BAS Mem to replace the conventional chromatographic membrane, we prepare batches of lateral-flow strips and achieve LFA of cTnI within 240 s, with a limit of detection of 1.97 ng mL −1 . The lateral-flow strips exhibit a specificity of 100%, a sensitivity of 93.3% in detecting 25 samples of suspected AMI patients. The lateral-flow strips show great performance in providing reliable results for clinical diagnosis, with the potential to provide early warnings for AMI.
研究领域:
生物医学工程
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心脏病学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2026
·
IF 11
Beibei Lu,Huaibin Gong,Hua Huang,Kuaifa Fang,Kun Yu,Bitao Lu,Guangqian Lan
Acute myocardial infarction (AMI) requires rapid diagnosis beyond conventional methods, which suffer from biological interference and complexity, limiting point-of-care (POC) application. To tackle these issues, we have devised an anthraquinone (AQ)-calibrated sensor unit coupled with smartphone-based analysis for universal detection of AMI biomarkers in untreated whole blood. Our novel dual-channel biosensing platform integrates AQ as an intrinsic reference standard, establishing a self-correction mechanism to effectively counteract hematological matrix influences. The platform enables swift quantification at pg/mL levels within 5 min without sample preprocessing, achieving detection limits of 0.3, 19.7, and 0.7 pg/mL for cTnI, Mb, and CK‑MB, respectively. The smartphone‑based intelligent analyzer automates real-time signal processing, creating a closed-loop POC diagnostic setup that shows promise for future at‑home AMI risk monitoring. This technological breakthrough showcases notable resilience to biological matrix interference while upholding analytical sensitivity comparable to clinical standards, representing a significant step toward decentralized cardiovascular emergency management.
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2023
·
IF 4.2
Huan Li,Qiwei Wang,Lili Shi,Tao Li
The elevated level of homocysteine (Hcy) in circulating blood is generally regarded as a risk factor for a variety of diseases including acute myocardial infarction (AMI), but there is no clear answer to whether circulating Hcy can be used for AMI diagnosis. To address it, here we have designed a tetraazacycle-based fluorescent probe for sensitive detection of endogenous Hcy in AMI patients’ serum and cardiomyocytes, showing a perfect selectivity over other biothiols (e.g. Cys and GSH). It mainly relies on the formation of a stable six-membered ring structure when this probe responds to Hcy, which is accompanied by a weakening of photoinduced electron transfer (PET) that induces a sharp increase in the fluorescence emission. In this way, Hcy can be probed in biofluids with high sensitivity. We then employed this fluorescent sensor to statistically analyze the levels of Hcy in human circulating blood, indicating a big difference between AMI patients and the healthy participants. To tell whether such a difference is applicable to AMI diagnosis, we further compare the expression levels of Hcy in cardiomyocytes and other tissue cells. It reveals a lower level of endogenous Hcy in cardiomyocytes, implying no direct relationship between the elevated Hcy and cardiomyocyte damage. This observation suggests that Hcy in circulating blood cannot be utilized as a potential biomarker for AMI diagnosis, although it is proven as a risk factor for this disease.
研究领域:
心脏病学
·
生物化学
·
医学诊断
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FLUORESCENCE
·
2023
·
IF 2.7
Xu Zichen,Xu Hedan,Duan Hongliang,Li Junjie,Hu Xiao,Jiang Kaixin,Wang Guixue,Zhang Yuanyuan
Simple and sensitive detection of cardiac biomarkers is of great significance for early diagnosis and prevention of acute myocardial infarction (AMI). Here, a ratiometric fluorescent nanohybrids probe (AuNCs-QDs) was synthesized through the coupling of bovine serum albumin-functionalized gold nanoclusters (AuNCs) with CdSe/ZnS quantum dots (QDs) to realize simple and sensitive detection of cardiac biomarker myoglobin (Mb). The AuNCs-QDs probe shows pink fluorescence under UV light, with two emission peaks at 468 nm and 630 nm belonging to QDs and AuNCs, respectively. Importantly, the presence of Mb caused fluorescence quenching of the blue-emitting QDs, thereby inhibiting the fluorescence resonance energy transfer (FRET) process between QDs and AuNCs, and reducing the fluorescence intensity ratio (F 468 /F 630 ) of AuNCs-QDs probe effectively. As the concentration of Mb increases, the ratiometric fluorescent probe also exhibits a visible fluorescence color change. The detection limit was as low as 4.99 μg/mL, and the response of the probe to Mb showed a good linear relationship up to 0.52 mg/mL. Moreover, the probe has excellent specificity for Mb. Besides, the AuNCs-QDs has been applied to detect Mb of urine samples. More importantly, we also developed an AuNCs-QDs probe modified smartphone-aided paper-based strip for on-site monitoring of Mb. As far as we know, this is the first report of a smartphone-aided paper-based strip for on-site quick monitoring of Mb, which provides a useful approach for AMI biomarker monitoring and may can be extended to other medical diagnostics.
研究领域:
生物传感器
·
纳米技术
·
医学诊断
·
心脏病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Applied Materials Today
·
2020
·
IF 8.352
Fanyi Huang,Lingyu Sun,Cheng Zhao,Zeping Qiu,Yuanjin Zhao,Wei Jin
As a severe cardiovascular disease, acute myocardial infarction (AMI) has been attaching increasing attention and research for its high morbidity and mortality. In this paper, the novel protein microcapsules integrated hierarchical scaffold is designed for treating AMI model by using microfluidic templates. Based on advanced microfluidic electrospray technique, core-shell microspheres with controllable size are fabricated to build a double barrier for improving the stability of encapsulated drug. These microspheres are further combined with an electrospun polymer membrane to construct the desired hierarchical structure. Based on these designs, the microcapsules can realize long-term drug release while the polymer membrane scaffold exhibits anti-adhesive effect in vitro . By decreasing the level of Angiotensin Ⅱ and inhibiting the Renin-Angiotensin System (RAS) pathway, ACE2 can increase the concentration of Ang (1–7) and enhance its protective function. To evaluate the therapeutic effect, ACE2-encapsulted hierarchical scaffold is applied to AMI mice model, which achieves regional sustainable release and improves the severity of ischemic lesion, with an additional utility of preventing post-operative adhesion. Compared with injecting intramyocardially, implanting hierarchical scaffold avoids excess injury and shows more obvious therapeutic efficacy. These properties suggest that our hierarchical cardiac scaffolds provide a potential drug administration approach for local treatment of AMI.
研究领域:
心血管疾病
·
生物材料
·
药物递送系统
·
组织工程
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Kaige Zhao,Min Tang,Huaisheng Wang,Zhenxian Zhou,Yafeng Wu,Songqin Liu
An immunosensing biochip for simultaneous detection of three biomarkers related to acute myocardial infarction (AMI) was developed based on anionic soybean peroxidase (SBP) functionalized nanoprobe and chemiluminescent imaging. The nanoprobes (Ab2-SiO 2 -SBP) were fabricated by co-immobilization of SBP and one of the detection polyclonal antibodies , anti-cardiac troponin I antigen (anti-cTnI), anti-creatine kinase-MB (anti-CK-MB) and anti-myoglobin (anti-Myo), on the silica nanoparticle surface. The detection sensitivity was enhanced since the large surface area of silica carriers increased the loading of SBP for per sandwiched immunoreaction. The immunosensing biochip designed as 3 × 8 wells array was constructed by simultaneously immobilizing three capture monoclonal antibodies on the same one microtiter well with 2 × 3 active spots. In the presence of target protein, the nanoprobes will be attached onto the spots with high specificity through the sandwiched immunoreactions, which triggered the chemiluminescence (CL) signals on each sensing site of the microtiter plates and allowed to CL imaging of three biomarkers in one well at the same time. Therefore, the proposed biochip was a promising convenient strategy for simultaneous detection of cTnI, CK-MB and Myo, which showed potential application for multianalyte determination in clinical diagnostics.
研究领域:
生物医学工程
·
临床诊断
·
纳米技术
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）