TALANTA
·
2015
·
IF 3.545
Yakun Lian,Hua Jiang,Jinzhou Feng,Xiaoyan Wang,Xiandeng Hou,Pengchi Deng
ATP , ADP and AMP are energy substances with vital biological significance. Based on the structural differences, a simple, rapid and comprehensive method has been established by 1 H and 31 P Nuclear Magnetic Resonance ( 1 H-NMR and 31 P-NMR) spectroscopies. Sodium 3-(trimethylsilyl) propionate-2,2,3,3-d 4 (TMSP) and anhydrous disodium hydrogen phosphate (Na 2 HPO 4 ) were selected as internal standards for 1 H-NMR and 31 P-NMR, respectively. Those three compounds and corresponding internal standards can be easily distinguished both by 1 H-NMR and 31 P-NMR. In addition, they all have perfect linearity in a certain range: 0.1–100 mM for 1 H-NMR and 1–75 mM for 31 P-NMR. To validate the precision of this method, mixed samples of different concentrations were measured. Recovery experiments were conducted in serum (91–113% by 1 H-NMR and 89–113% by 31 P-NMR).
研究领域:
生物化学
·
医学检测
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DOI
PubMed
高影响因子
ENERGY & FUELS
·
2025
·
IF 5.3
Runfen Zheng,Lixin Wei,Yuxin Bai,Jianfei Man,Jian Ye,Huiping Zhang,Yihan Wang
To address the issues of poor regeneration performance and high regeneration energy consumption associated with traditional CO2 absorbents, the development of novel phase-change absorbents has become a key area of research. In this study, a new phase-change absorbent system, DEGDEE-AEEA-AMP, is developed, using N-(2-hydroxyethyl)ethylenediamine (AEEA) as the main absorbent, bis(2-ethoxyethyl)ether (DEGDEE) as the phase-separation agent, 2-amino-2-methyl-1-propanol (AMP) as the activator, and water as the solvent. The performance of this system is compared with that of monoethanolamine (MEA) at a 30% mass concentration, which serves as the reference standard. The study includes absorption, desorption, corrosion, cyclic absorption–desorption experiments, and regeneration energy consumption estimations. The results indicate that the volume of the rich phase in the DEGDEE-AEEA-AMP (DAP) system constitutes 56.7% of the total volume. When the AMP mass fraction is 15%, the absorption capacity reaches 2.52 mol·kg–1, which is 14.0% higher than that of MEA. The maximum desorption rate is 13.19 × 10–3 mol·kg–1·s–1, which is 3.39 times higher than that of MEA. The minimum regeneration energy consumption is 2.742 GJ·t–1 CO2, representing a 29.9% reduction compared to 30% MEA. The corrosion rate of the rich liquid is 0.230 mm/a, 1.32 times that of MEA, but it can be significantly reduced by adding anhydrous Na2SO3. When the AMP mass fraction is 15%, the DAP phase-change absorbent system exhibits high absorption and desorption performance, good cyclic stability, and lower regeneration energy consumption.
研究领域:
化学工程
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
RENEWABLE ENERGY
·
2022
·
IF 8.634
Naveed Ahmad,Ying Chen,Xiaoxiao Wang,Peixu Sun,Yuting Bao,Xia Xu
Electrochemical CO 2 reduction reaction (ECO 2 RR) offers an eco-friendly way to produce value-added products by utilizing the waste CO 2 from the environment. Recently, amines have been utilized as electrolytes for direct conversion of CO 2 but processes were energy-intensive and lack selectivity due to the formation of highly stable carbamate. 2-amino-2-methyl-1-propanol (AMP) is a sterically hindered amine , produces less stable carbamate, and generates more HCO 3 − . Herein, ECO 2 RR performance using AMP aqueous solution as electrolyte was investigated over Ag, Cu, and Zn metal electrodes. Ultimately, 91 ± 7% CO selectivity on HCl modified polycrystalline Ag electrode at −0.91 V vs RHE in 1 M AMP aqueous solution in the presence of CTAB was achieved, 1.7 times greater than in 1 M aqueous ethanolamine (MEA) solution. Synergetic effects of unstable AMP-carbamate, generation of nano-agglomerates on pitted/porous Ag surface arising from HCl treatment, and a compact electrical double layer due to CTAB present are responsible for the high selectivity of CO. Our findings provide the understanding of electrode-electrolyte interactions and offer a facile strategy to directly convert CO 2 to CO from CO 2 capture solutions.
研究领域:
环境科学
·
电化学
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DOI
高影响因子
Journal of Cleaner Production
·
2023
·
IF 11.1
Jiayi Li,Weixing Han,Heyao Liu,Minhua Su,Diyun Chen,Gang Song
The development and use of clean energy produced by nuclear power plants is becoming a major international trend, but it will inevitably lead to the problem of radionuclide pollution. Radioactive nuclear wastewater containing Cs and U and produced as a byproduct of coastal nuclear power production can cause substantial environmental pollution . In order to simultaneously remove radioactive cesium and uranium, polydopamine (PDA) inlaid ammonium phosphomolybdate (AMP) has established a hydrophilic functional structural film on the surface of Fe 3 O 4 , forming AMP@PDA@Fe 3 O 4 (APF) which is used to simultaneous remove Cs(I) and U(VI). Using the pH sensitive range of APF adsorption in the process of adsorbing Cs(I) and U(VI), synergistic adsorption can be achieved under the condition of weak acidity to neutral. The effects of temperature, and common cations on Cs(I) and U(VI) adsorption by APF were investigated, the adsorption was found to be sensitive to the presence of K + . The efficiency of Cs(I) and U(VI) adsorption by APF was 99.88% and 98.39%, respectively. The Cs(I) adsorption process was primarily ion exchange with NH 4 + within APF composite, whereas that for U(VI) was primarily C-O complexation. Both the Cs(I) and U(VI) adsorption reactions were consistent with second-order kinetic and Langmuir isothermal adsorption models. After five cycles of experiments the removal efficiency of Cs(I) and U(VI) can reach 64.88% and 79.81%, respectively. APF was also found to have high selectivity for Cs and U in seawater with high salt content at pH 6.0–7.0. In mimic contaminated seawater, the efficiency of Cs(I) and U(VI) removal was 97.65% and 84.63%, respectively. These findings provide references for the remove of Cs(I) and U(VI) from radioactive wastewater , making the process of energy production safer.
研究领域:
环境科学
·
材料科学
·
核能工程
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Sciences
·
2023
·
IF 6.9
Xiaoyun Chen,Guohua Jing,Bihong Lv,Zuoming Zhou
The non -aqueous solid-liquid biphasic solvent of 2-amino-2-methyl-1-propanol (AMP)/piperazine (PZ)/dipropylene glycol dimethyl ether (DME) features a high CO 2 absorption loading, favorable phase separation behavior and high regeneration efficiency. Different with the liquid-liquid phase change solvent, the reaction kinetics of CO 2 capture into solid-liquid biphasic solvent was rarely studied. In the present work, the reaction kinetics of CO 2 absorption into AMP/PZ/DME solid-liquid biphasic solvent was investigated into the double stirred kettle reactor. The absorption reaction followed a pseudo-first-order kinetic model according to the zwitterion mechanism. The overall reaction rate constant ( k ov ) and the enhancement factor ( E ) of CO 2 absorption both increased with increasing temperature. The total mass transfer resistance of the absorbent decreased with increasing temperature and increased with increasing absorption loading, so the higher reaction temperature was conducive to the absorption, and the liquid phase mass transfer resistance was the main factor affecting the absorption rate.
研究领域:
化学工程
·
环境科学
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产品关联
DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2021
·
IF 3.493
Caixia Cui,Mengyuan Kong,Yihan Wang,Chenyan Zhou,Hong Ming
Polyphosphate kinase (PPK) is important for industrial processes involving ATP regeneration. While a variety of methods have been reported for regenerating ATP from ADP, few have explored enzyme catalyzed ATP regeneration from cheaper and stable AMP. In this work, PPKs from different sources were expressed and their catalytic activity were tested at different reaction temperatures, reaction pH and with different polyphosphate (polyP n ) types. The ATP regeneration system for glutathione (GSH) synthesis was established using a single PPK capable of phosphorylating AMP to synthesize ATP from AMP and short chain polyP n . GSH yield was obtained using adenosine mono-, di- and triphosphates, which confirmed the flexibility of our constructed ATP regeneration system coupled with GSH synthesis via bifunctional GSH synthase. Finally, optimization of the GSH synthesis yielded conversion value above 80 %. Overall, these results illustrate that PPK is suitable for a broader range of substrates than previously expected, and has great untapped potential for applications involving ATP regeneration.
研究领域:
生物化学
·
生物技术
·
工业生物处理
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产品关联
DOI
PubMed
高影响因子
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
·
2024
·
IF 4.1
Junyao Wang,Xi Liu,Yuxin Song,Zhonghua Liu,Xing Tang,Huaxin Tan
Antibiotic resistance has become a critical concern in recent years, and antimicrobial peptides may function as innovative antibacterial agents to address this issue. In this work, we identified a novel antimicrobial peptide, LC-AMP-I1, derived from the venom of Lycosa coelestis, demonstrating substantial antibacterial properties and minimal hemolytic activity. LC-AMP-I1 was subjected to additional assessment for antibacterial efficacy, anti-biofilm properties, drug resistance, stability, and cytotoxicity in vitro. It exhibited comparable antibacterial efficacy to melittin against six common clinical multidrug-resistant bacteria, effectively inhibiting biofilm formation and disrupting established biofilms. Additionally, LC-AMP-I1 demonstrated minimal bacterial resistance, excellent stability, negligible mammalian cell toxicity, low hemolytic activity, and appropriate selectivity for both normal and tumor cells. When combined with traditional antibiotics, LC-AMP-I1 exhibited additive or synergistic therapeutic effects. In a neutropenic mouse thigh infection model, LC-AMP-I1 exhibited a therapeutic effect in inhibiting bacterial proliferation in vivo. The mechanistic investigation indicated that LC-AMP-I1 could influence bacterial cell membrane permeability at low concentrations and directly disrupt structure-function at high concentrations. The results of this work indicate that LC-AMP-I1 may function as a viable alternative to traditional antibiotics in addressing multidrug-resistant bacteria.
研究领域:
微生物学
·
分子生物学
·
药物开发
·
感染性疾病
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产品关联
DOI
PubMed
高影响因子
LUMINESCENCE
·
2017
·Vol. 32
·期 7
·1277-1282
·
IF 1.509
Keying Cai,Mulan Zeng,Fenfen Liu,Nan Liu,Zhenzhong Huang,Yonghai Song,Li Wang
An easy and effective strategy for synthesizing a ratiometric fluorescent nanosensor has been demonstrated in this work. Novel fluorescent BSA–AuNPs@Tb–AMP (BSA, bovine serum albumin; AMP, adenosine 5′-monophosphate; AuNPs, Au nanoparticles) metal–organic framework (MOF) nanostructures were synthesized by encapsulating BSA–AuNPs into Tb–AMP MOFs for the detection of 2,6-pyridinedicarboxylic acid (DPA) and Hg 2+ . DPA could strongly co-ordinate with Tb 3+ to replace water molecules from the Tb 3+ center and accordingly enhanced the fluorescence of Tb–AMP MOFs. The fluorescence of BSA–AuNPs at 405 nm remained constant. While the fluorescence of BSA–AuNPs at 635 nm was quenched after Hg 2+ was added, the fluorescence of Tb–AMP MOFs remained constant. Accordingly, a ratiometric fluorescence nanosensor was constructed for detection of DPA and Hg 2+ . The ratiometric nanosensor exhibited good selectivity to DPA over other substances. The F 545 /F 405 linearly increased with increase of DPA concentration in the range of 50 nM to 10 μM with a detection limit as low as 17.4 nM. F 635 /F 405 increased linearly with increase of Hg 2+ concentration ranging from 50 nM to 1 μM with a detection limit as low as 20.9 nM. Additionally, the nanosensor could be successfully applied for the determination of DPA and Hg 2+ in running water.
研究领域:
材料科学
·
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2024
·
IF 3.1
Li Keyan,Deng Fuli,Wang Yonghua,Wang Fanghua
Activation of fatty acids as acyl-adenylates by fatty acid-AMP ligase (FAAL) is a well-established process contributing to the formation of various functional natural products. Enzymatic characterization of FAALs is pivotal for unraveling both the catalytic mechanism and its role in specific biosynthetic pathways. In this study, we recombinantly expressed and characterized a novel FAAL derived from marine Pseudoalteromonas citrea ( Pc FAAL). Pc FAAL was a cold-adapted neutral enzyme, demonstrating optimal activity at 30 °C and pH 7.5. Notably, its specific activity relied on the presence of Mg 2+ ; however, higher concentrations exceeding 10 mM resulted in inhibition of enzyme activity. Various organic solvents, especially water-immiscible organic solvents, demonstrated an activating effect on the activity of Pc FAAL on various fatty acids. The specific activity exhibited a remarkable 50-fold increase under 4% (v/v) n-hexane compared to the aqueous system. Pc FAAL displayed a broad spectrum of fatty acid substrate selectivity, with the highest specific activity for octanoic acid (C8:0), and the catalytic efficiency ( k cat / K m ) for octanoic acid was determined to be 1.8 nM −1 ·min −1 . Furthermore, the enzyme demonstrated biocatalytic promiscuity in producing a class of N-acyl amino acid natural products, as verified by LC-ESI MS. Results indicated that the Pc FAAL exhibits promiscuity towards 10 different kinds of amino acids and further demonstrated their potential value in the biosynthesis of corresponding functional N-acyl amino acids.
研究领域:
海洋微生物学
·
酶学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NUCLEAR MATERIALS
·
2019
·
IF 2.547
Hui Dan,Qiang Xian,Li Chen,Tianheng Xiong,Zhengdi Jiang,Facheng Yi,Yi Ding
Mesoporous ammonium molybdophosphate/SBA-15 (AMP/SBA-15) was considered as an excellent adsorbent to remove Cs + from radioactive wastewater. However, AMP/SBA-15 obtained from two-step method suffers from poor stability and low reuse ability. Herein, we reported a facile one-step method to fabricate AMP/SBA-15 using Phosphomolybdic acid (PMA) as acid media. The effect of PMA content on the properties of the resultant AMP/SBA-15 was investigated in detail. And the Cs + adsorption performance of the developed AMP/SBA-15 adsorbents was also evaluated. It was found that the PMA content had a strong influence on the structure and morphology of AMP/SBA-15. The mesoporous structure order of the AMP/SBA-15 decreased with increasing PMA content. The AMP particles were mainly incorporated into the framework of SBA-15. Furthermore, the AMP incorporation rate obtained from one-step method is much higher than that of two-step method. The developed AMP/SBA-15 with AMP in the framework of SBA-15 exhibited good stability and high reuse ability for the removal of Cs + .
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Wenjun Qu,Huili Wang,Guodong Li,Zhaoping Song,Xiaona Liu,Fengshan Zhang,Wenxia Liu,Dehai Yu,Dandan Ji
The removal of heavy metal ions and medical antibiotics from wastewater is crucial for environmental protection and human health. However, the adsorption capacities and reusability of previously reported adsorbent materials have been insufficient for efficient and sustainable wastewater treatment . In this study, we present a composite material, UiO-66-AMP@PAN, synthesized from metal-organic frameworks (MOFs) and polyacrylonitrile (PAN), which exhibits superior adsorption properties and reusability. The material demonstrates excellent adsorption capacities for heavy metal ions Pb(II) and Cr(VI) as well as the antibiotic tetracycline hydrochloride (TCH), among which the maximum adsorption capacities for Pb(II)(pH=7) and Cr(VI)(pH=5) and antibiotic tetracycline hydrochloride (TCH) (pH=9) were 455.8 mg/g, 442.08 mg/g and 405.92 mg/g, respectively. The adsorption efficiency of UiO-66-AMP@PAN as an adsorbent for adsorbing Pb(II)(pH=7) and Cr(VI)(pH=5) and the antibiotic tetracycline hydrochloride (TCH) (pH=9) remained above 90% even after ten consecutive cycles. The performance of UiO-66-AMP@PAN as an adsorbent has remained unchanged across multiple adsorption-desorption cycles, demonstrating its potential for long-term use in wastewater treatment applications. This innovative approach paves the way for the development of more efficient and environmentally friendly adsorption materials to address the challenges posed by heavy metal ions in wastewater and medical antibiotics.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Ginseng Research
·
2021
·
IF 6.06
Jingui Hu,Ling Zhang,Fei Fu,Qiong Lai,Lu Zhang,Tao Liu,Boyang Yu,Junping Kou,Fang Li
Background Ginsenoside Rb1, a bioactive component isolated from the Panax ginseng, acts as a remedy to prevent myocardial injury. However, it is obscure whether the cardioprotective functions of Rb1 are related to the regulation of endogenous metabolites, and its potential molecular mechanism still needs further clarification, especially from a comprehensive metabolomics profiling perspective. Methods The mice model of acute myocardial ischemia (AMI) and oxygen glucose deprivation (OGD)-induced cardiomyocytes injury were applied to explore the protective effect and mechanism of Rb1. Meanwhile, the comprehensive metabolomics profiling was conducted by high-performance liquid chromatography and quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS) and a tandem liquid chromatography and mass spectrometry (LC-MS). Results Rb1 treatment profoundly reduced the infarct size and attenuated myocardial injury. The metabolic network map of 65 differential endogenous metabolites was constructed and provided a new inspiration for the treatment of AMI by Rb1, which was mainly associated with mitophagy. In vivo and in vitro experiments, Rb1 was found to improve mitochondrial morphology, mitochondrial function and promote mitophagy. Interestingly, the mitophagy inhibitor partly attenuated the cardioprotective effect of Rb1. Additionally, Rb1 markedly facilitated the phosphorylation of AMP-activated protein kinase α (AMPKα), and AMPK inhibition partially weakened the role of Rb1 in promoting mitophagy. Conclusions Ginsenoside Rb1 protects acute myocardial ischemia injury through promoting mitophagy via AMPKα phosphorylation, which might lay the foundation for the further application of Rb1 in cardiovascular diseases.
研究领域:
药理学
·
心脏病学
·
代谢组学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）