TALANTA
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2024
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IF 5.6
Zhigang Yu,Yangkejia Xiang,Xianda Han,Yi Guang,Fengqin Li
A photo/electrochemical coupling interface of Ru[dcbpy] 3 2+ -AMT/Au (AMT; 5-Amino-1,3,4-thiadiazole-2-thiol) was fabricated using a dehydration condensation sulfhydrating method. For the interface functional properties, a combined dual-signal recording (CDSR) method was applied to characterize the response characteristics, and a scanning electrochemical microscopy-electrochemiluminescence (SECM-ECL) imaging was developed to assess the interface distribution uniformity. The interface biosensing compatibility was validated by constructing a simple DNA sensor. The research results show that the interaction between the two functional parameters follows a synergistic effect mechanism in the coupling conditions and an interference effect mechanism in the detection condition. Under optimized conditions, the saturation dual-signal response values are 156.0 and 86.8 μA, respectively. The statistics and imaging comparison analysis validate good interface distribution uniformity and stability performance. The DNA sensor's dual-signal detection limits to the signal probe (SP) are ∼30 fM and 0.3 pM with linear ranges of 100.0 fM ∼ 1.0 nM and 1.0 pM ∼ 10.0 nM, respectively. The fabricated interface exhibits an effective bi-functional response performance compatible with biosensing. The proposed imaging method has a high technical fit for studying photo/electrochemical coupling interfaces and can also provide a reference for other similar coupling interface analyses.
研究领域:
生物传感
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分析化学
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电化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2021
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IF 6.165
Bingqiao Yang,Pengliang Huang,Qing An
A small molecular triazole derivative called AMT (3-amino-5-mercapto-1,2,4-triazole) was explored as a novel and efficient chalcopyrite depressant in the preferential flotation of chalcopyrite from molybdenite. The flotation performances were comprehensively investigated via micro-flotation tests of singe and mixed minerals with and without Cu collector. The adsorption mechanisms were determined in terms of contact angle, adsorption capacity, and zeta potential, Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and DFT calculations. Single mineral flotation results illustrated that the AMT selectively depressed chalcopyrite over a wide range of pH values. Particularly, AMT was able to depress chalcopyrite even in the presence of sodium isobutyl xanthate (SIBX). The contact angle, adsorption capacity, zeta potential, and FTIR results suggested that AMT was preferentially adsorbed on chalcopyrite surface, but it barely interacted with molybdenite. XPS tests and DFT calculations demonstrated that AMT was chemically adsorbed on chalcopyrite surface through the hybridization of S (1) and N (1) 2p orbitals of AMT with the Cu (1) 3d orbital. The bonding between S (1) atom and Cu (1) was much stronger than that between N (1) and Cu (1). Thus, AMT exhibited good potential as a novel depressant due to its excellent selectivity and practicability.
研究领域:
矿物加工工程
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化学工程
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材料科学
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产品关联
DOI
高影响因子
MOLECULAR PHARMACOLOGY
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2025
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IF 3
Shixin Li,Jingyan Wei,Shaofen Zhou,Shuaiqi Ma,Peihang Jiang,Jian He
The rise of drug-resistant influenza A virus (IAV) strains and severe lung injury caused by excessive inflammation highlight the need for new antivirals that combine antiviral and immunomodulatory effects. Adamantane, an established anti-IAV agent, targets the viral M2 matrix protein, but widespread mutations have limited its clinical utility. Previously, we identified that 2,5-dihydroxybenzylalcohol and its derivatives possess anti-IAV activity. In this study, we synthesized a series of adamantane-2,5-dihydroxy-benzylalcohol derivatives via reductive amination. Among these, Amt-1 demonstrated strong antiviral activity against both amantadine-sensitive (H1N1 PR8) and clinical isolates IAV strains (-log IC 50 =6.12–6.51 M). Mechanistic analyses, including immunofluorescence, ITC, qPCR, ELISA, luciferase assays, and Western blotting, revealed that Amt-1 not only interacts with the peptide segment of M2 ion channel protein (-log Kd = 5.08 M) but also activates Nrf2, leading to increased HO-1 expression and reduced production of pro-inflammatory cytokines IL-6, IL-1β, and TNF-α. Additionally, Amt-1 inhibited IAV-induced NF-κB activation, thereby suppressing viral replication. In vivo , Amt-1 reduced lung viral titers and mitigated histopathological damage in mice, surpassing amantadine in controlling inflammation and increased survival to 33%. In summary, Amt-1 is the first adamantane derivative shown to exert anti-IAV effects by modulating Nrf2/HO-1-mediated anti-inflammatory pathways and NF-κB-driven antiviral responses, while retaining M2 ion channel inhibition. This host-directed mechanism addresses drug resistance and limits immunopathology, making Amt-1 a promising therapeutic candidate for resistant influenza infections.
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
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2016
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IF 3.035
Jie-hua Shi,Dong-qi Pan,Xiou-xiou Wang,Ting-Ting Liu,Min Jiang,Qi Wang
Artemether (AMT), a peroxide sesquiterpenoides, has been widely used as an antimalarial for the treatment of multiple drug-resistant strains of plasmodium falciparum malaria . In this work, the binding interaction of AMT with bovine serum albumin (BSA) under the imitated physiological conditions (pH 7.4) was investigated by UV spectroscopy , fluorescence emission spectroscopy , synchronous fluorescence spectroscopy, Fourier transform infrared spectroscopy (FT-IR), circular dichroism (CD), three-dimensional fluorescence spectroscopy and molecular docking methods. The experimental results indicated that there was a change in UV absorption of BSA along with a slight red shift of absorption wavelength, indicating that the interaction of AMT with BSA occurred. The intrinsic fluorescence of BSA was quenched by AMT due to the formation of AMT–BSA complex. The number of binding sites ( n ) and binding constant of AMT–BSA complex were about 1 and 2.63 × 10 3 M − 1 at 298 K, respectively, suggesting that there was stronger binding interaction of AMT with BSA. Based on the analysis of the signs and magnitudes of the free energy change (Δ G 0 ), enthalpic change (Δ H 0 ) and entropic change (Δ S 0 ) in the binding process, it can be concluded that the binding of AMT with BSA was enthalpy-driven process due to | Δ H °| > | T Δ S °|. The results of experiment and molecular docking confirmed the main interaction forces between AMT and BSA were van der Waals force . And, there was a slight change in the BSA conformation after binding AMT but BSA still retains its secondary structure α-helicity. However, it had been confirmed that AMT binds on the interface between sub-domain IIA and IIB of BSA.
研究领域:
药理学
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生物化学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
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