JOURNAL OF FOOD SCIENCE
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2014
·Vol. 79
·期 10
·C1886
·
IF 1.791
Lingyi Liu,Boneng Xia,Cheng Jin,Yu Zhang,Ying Zhang
en This link goes to a English section pt This link goes to a English section Antioxidant of bamboo leaves (AOB) is a novel natural food antioxidant approved in China since 2004. Natural phenolics contained in the current AOB are usually polyhydroxy derivatives exhibiting hydrophilic character, which has been marked as water-soluble AOB (AOB-w). In order to broaden the application fields, oil-soluble AOB (cAOB-o) was obtained by chemical acylation of AOB-w with different chain-length fatty acids varying from C8 to C18. Results indicated that the yield and solubility of cAOB-o in 1-octanol solvent depended on the carbon chain length of acyl donor, and cAOB-o derived from C12 fatty acid exhibited the more powerful antioxidant activity evaluated by β-carotene/linoleic acid bleaching assay. Total phenolic content decreased by Folin–Ciocalteu assay. Fourier transform infrared spectra showed the increase of a carbonyl (C = O) peak at 1701 cm −1 and a decrease in the intensity of the absorption at 3400 cm −1 (O-H stretching) in cAOB-o. Acylation was inferred to mainly occur on the hydroxyl groups of flavones C -glycosides according to the change of high-performance liquid chromatography spectra and the contents of total flavonoids and phenolic acids. cAOB-o with the addition of 0.02% significantly increased oxidative stability of palm oil 1.59 times, lard 3.74 times, and fried potato chips 2.08 times, which was better than the effect of oil-soluble tea polyphenol ( P < 0.01). Moreover, cAOB-o was identified to be actually nontoxicity by an acute oral toxicity test. All the above results indicated that cAOB-o could be used as a novel and effective oil-soluble antioxidant in the food industry. Practical Application en This link goes to a English section pt This link goes to a English section Oil-soluble antioxidant of bamboo leaves (cAOB-o) was obtained by chemical acylation with water-soluble AOB (AOB-w) and different chain-length fatty acids. The solubility of cAOB-o in 1-octanol solvent increased sharply. cAOB-o was able to increase the oxidative stability of palm oil, lard, and fried potato chips. cAOB-o has been identified to be actually not toxic by acute oral toxicity test. All the results indicated that cAOB-o could be used as an effective oil-soluble antioxidant in the food industry.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Jian Zhao,Shangbiao Fang,Weixiao Qi,Huijuan Liu,Jiuhui Qu
Biodegradation is regarding as the most important organic micro-pollutants (OMPs) removal mechanism during riverbank filtration (RBF), but the OMPs co-metabolism mechanism and the role of NH 4 + -N during this process are not well understood. Here, we selected atenolol as a typical OMP to explore the effect of NH 4 + -N concentration on atenolol removal and the role of ammonia oxidizing bacteria (AOB) in atenolol biodegradation. The results showed that RBF is an effective barrier for atenolol mainly by biodegradation and adsorption. The ratio of biodegradation and adsorption to atenolol removal was dependent on atenolol concentration. Specifically, atenolol with low concentration (500 ng/L) is almost completely removed by adsorption, while atenolol with higher concentration (100 μg/L) is removed by biodegradation (51.7%) and adsorption (30.8%). Long-term difference in influent NH 4 + -N concentrations did not show significant impact on atenolol (500 ng/L) removal, which was mainly dominated by adsorption. Besides, AOB enhanced the removal of atenolol (100 μg/L) as biodegradation played a more crucial role in removing atenolol under this concentration. Both AOB and heterotrophic bacteria can degrade atenolol during RBF, but the degree of AOB's contribution may be related to the concentration of atenolol exposure. The main reactions occurred during atenolol biodegradation possibly includes primary amide hydrolysis, hydroxylation and secondary amine depropylation. About 90% of the bio-transformed atenolol was produced as atenolol acid. AOB could transform atenolol to atenolol acid by inducing primary amide hydrolysis but failed to degrade atenolol acid further under the conditions of this paper. This study provides novel insights regarding the roles played by AOB in OMPs biotransformation during RBF.
研究领域:
环境科学
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微生物学
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PubMed
高影响因子
ANNALS OF BOTANY
·
2023
·Vol. 131
·期 4
·723-736
·
IF 4.2
Li Hu-Jun,Bai Wan-Peng,Liu Lin-Bo,Liu Hai-Shuang,Wei Li,Garant Timothy M,Kalinger Rebecca S,Deng Yu-Xuan,Wang Gai-Ni,Bao Ai-Ke,Ma Qing,Rowland Owen,Wang Suo-Min
Background and AimsDesert plants possess excellent water-conservation capacities to survive in extreme environments. Cuticular wax plays a pivotal role in reducing water loss through plant aerial surfaces. However, the role of cuticular wax in water retention by desert plants is poorly understood.MethodsWe investigated leaf epidermal morphology and wax composition of five desert shrubs from north-west China and characterized the wax morphology and composition for the typical xerophyte Zygophyllum xanthoxylum under salt, drought and heat treatments. Moreover, we examined leaf water loss and chlorophyll leaching of Z. xanthoxylum and analysed their relationships with wax composition under the above treatments.Key ResultsThe leaf epidermis of Z. xanthoxylum was densely covered by cuticular wax, whereas the other four desert shrubs had trichomes or cuticular folds in addition to cuticular wax. The total amount of cuticular wax on leaves of Z. xanthoxylum and Ammopiptanthus mongolicus was significantly higher than that of the other three shrubs. Strikingly, C31 alkane, the most abundant component, composed >71 % of total alkanes in Z. xanthoxylum, which was higher than for the other four shrubs studied here. Salt, drought and heat treatments resulted in significant increases in the amount of cuticular wax. Of these treatments, the combined drought plus 45 °C treatment led to the largest increase (107 %) in the total amount of cuticular wax, attributable primarily to an increase of 122 % in C31 alkane. Moreover, the proportion of C31 alkane within total alkanes remained >75 % in all the above treatments. Notably, the water loss and chlorophyll leaching were reduced, which was negatively correlated with C31 alkane content.ConclusionZygophyllum xanthoxylum could serve as a model desert plant for study of the function of cuticular wax in water retention because of its relatively uncomplicated leaf surface and because it accumulates C31 alkane massively to reduce cuticular permeability and resist abiotic stressors.
研究领域:
植物学
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生态学
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PubMed
高影响因子
ANNALS OF BOTANY
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2025
·
IF 3.6
Gao Yan,Phan Thi Kim Lien,Zhang Jiawei,Yu Ying,Wang Chao
Backgrounds and AimsFasciclin-like arabinogalactan proteins (FLAs) function in diverse plant physiological processes, particularly cell wall biosynthesis and tension wood (TW) formation. This study aimed to identify Betula platyphylla FLA genes associated with secondary xylem and TW development, thereby elucidating molecular mechanisms underlying xylogenesis to facilitate molecular improvement strategies.MethodsTissue-specific expression of two FLA genes (BpFLA1, BPFLA2) was analyzed via quantitative real-time PCR. BpFLA1/2-overexpressing transgenic lines and bpfla1 knockout mutants were generated using the pROKⅡ vector and CRISPR-Cas9 technology. Anatomical phenotypes, cell wall composition, and TW development were subsequently investigated.Key ResultsTwo FLA genes (BpFLA1, BpFLA2) were identified in the birch TW transcriptome. Phylogenetically, BpFLA1 and BpFLA2 exhibit conserved evolutionary relationships and structural domains. Both genes were highly expressed in mature stems but suppressed by mechanical stimuli. BpFLA1/2-overexpressing (OE) transgenics displayed slightly suppressed growth, reduced cell wall thickness, enlarged xylem vessel area, and notably inhibited TW formation following artificial bending. Conversely, bpfla1 knockout mutants exhibited significant thickening of xylem fiber cell walls—a phenotype contrasting sharply with OE lines.ConclusionBpFLA1/2 functions as a critical regulator of cell wall development and a negative regulator of xylem fiber and TW formation in birch. Therefore, this study identifies BpFLA1/2 as a novel target for genetic improvement. BpFLA1/2 overexpression potentially enhances wood straightness and quality, whereas its suppression or knockout may benefit cellulose production for pulpwood applications.
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PubMed
高影响因子
ANNALS OF BOTANY
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2024
·
IF 3.6
Guo Huanhuan,Li Shiqin,Liu Yong,Yang Qinsong
Background and AimsChinese pistachio (Pistacia chinensis Bunge), an important horticultural plant species, holds great ornamental value with beautiful leaves and fruits. Seedling propagation of this tree species is restricted by its erratic seed germination, however, the germination mechanism is ambiguous yet. The aim of this study was to figure out the germination mechanism from the novel perspective based on the multi-omics data.MethodsThe multi-omics technique combined with hormone content measurement was first applied in seed germination of Chinese pistachio.Key ResultsDue to the great accumulation during seed germination, catechin stood out from the identified metabolites by broadly targeted metabolomic analysis. Exogenous catechin of 10 mg/L significantly improved the germination of Chinese pistachio seeds. An interesting result of hormone analysis showed that the improving effect of catechin could be attributed to increase of the gibberellic acid 3 (GA3) content rather than decrease of the abscisic acid (ABA) content before germination. The paclobutrazol (PAC, a GA biosynthesis inhibitor) and PAC + catechin treatments also showed that the promoting effect of catechin on seed germination depends on GA biosynthesis. Transcriptome analysis and qRT‒PCR further revealed that catechin induced the expression of PcGA20ox5 to activate GA biosynthesis. Several transcription factors were induced by catechin and GA treatments, such as TCP, bZIP and C3H, which may play an important regulatory role in GA biosynthesis in a catechin-mediated way.ConclusionsCatechin promotes seed germination via GA biosynthesis in Chinese pistachios. This study proposes a novel mechanism by which catechin promotes seed germination via the GA pathway, which provides new insight into a comprehensive understanding of seed dormancy and germination.
研究领域:
植物学
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植物生理学
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分子生物学
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园艺学
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PubMed
高影响因子
Frontiers in Microbiology
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2022
·
IF 6.064
Cao Xiaoxu,Zhao Wei,Zhang He,Lin Jitong,Hu Jingying,Lou Yanhong,Wang Hui,Yang Quangang,Pan Hong,Zhuge Yuping
Introduction: The large-scale development of animal husbandry and industrialization lead to more and more serious co-contamination from heavy metals and antibiotics in soils. Ecotoxic effects of residues from antibiotics and heavy metals are of increasing concern.Materials and Methods: In this study, oxytetracycline (OTC) and cadmium (Cd) were selected as target pollutants to evaluate the individual and combined effects on nitrification process using four different soil types sampled from North to South China through a 56-day incubation experiment.Results and Discussion: The results demonstrated that the contaminations of OTC and Cd, especially combined pollution had significant inhibitory effects on net nitrification rates (NNRs) as well as on AOA and AOB abundance. The toxic effects of contaminants were greatly enhanced with increasing OTC concentration. AOB was more sensitive than AOA to exogenous contaminants. And the interaction effects of OTC and Cd on ammonia oxidizers were mainly antagonistic. Furthermore, Cd contaminant (with or without OTC) had indirect effects on nitrification activity via inhibiting mineral N and AOA/AOB, while OTC alone indirectly inhibited nitrification activity by inhibiting ammonia oxidizers. The results could provide theoretical foundation for exploring the eco-environmental risks of antibiotics and heavy metals, as well as their toxic effects on nitrification processes.
研究领域:
环境科学
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土壤学
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生态毒理学
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PubMed
高影响因子
Journal of Water Process Engineering
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2022
·
IF 7.34
Qianqian Pei,Ming Chen,Jingda Li,Jie Liu,Nan Wu,Kaiyang Chen,Xiaoyu Chen,Yanli Liu,Yifeng Feng,Gaofeng Ren,Xiaoying Liu
Ammonia oxidation is a critical process during nitrogen removal, while the activity of ammonia oxidizing bacteria (AOB) might be impacted severely in disposing high-salinity nitrogen-containing wastewater. To protect AOB in high saline medium, layer-by-layer self-assembly using graphene oxide (GO) and poly(diallyl dimethyl ammonium chloride) (PDDA) was applied to immobilize enriched AOB. By mixing GO and AOB, it was found that 30 mg/L GO showed the best promotion on the ammonia oxidation capacity of AOB. After coating, AOB with PDDA/GO showed higher absolute value of zeta potential of −20.1 ± 1 mV and compact structure. Comparing with uncoated AOB, although NH 4 + -N consumption process was slightly inhibited by the PDDA/GO composite materials under low-salinity conditions (0–2 % NaCl), they presented great effective protection on the capacity of AOB in a 3 % NaCl solution. The consumption ratio of NH 4 + -N was 1.34 times that of uncoated AOB, and the accumulation ratio of NO 2 − -N of the former one was 1.40 times that of the latter. It inferred that the inhibitory to mass transferring caused by immobilizing materials was less than its positive promotion of AOB protection at 3 % NaCl concentration, and the protection of PDDA/GO composite materials was better than GO. This finding has some certain guiding significance for ammonia oxidation in high salinity.
研究领域:
环境微生物学
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污水处理工程
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DOI
高影响因子
CHEMOSPHERE
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2023
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IF 8.8
Weiping Chen,Bingzheng Wang,Yaqing Wang,Ji Li
Antibiotics are widely used drugs in the world and pose serious threats to ecosystems and human health. Although it has been reported that ammonia oxidizing bacteria (AOB) can cometabolize antibiotics, little has been reported on how AOB would respond to the exposure of antibiotics on extracellular and enzymatic levels, as well as the impact of antibiotics on the bioactivity of AOB. Therefore, in this study, a typical antibiotic, sulfadiazine (SDZ), was selected, and a series short-term batch tests using enriched AOB sludge were conducted to investigate the intracellular and extracellular responses of AOB along the cometabolic degradation process of SDZ. The results showed the cometabolic degradation of AOB made the main contribution to SDZ removal. When the enriched AOB sludge was exposed to SDZ, ammonium oxidation rate, ammonia monooxygenase activity, adenosine triphosphate concentration and dehydrogenases activity were negatively affected. The amoA gene abundance increased 1.5 folds within 24 h, which may enhance the uptake and utilization of substrates and maintain stable metabolic activity. In the tests with and without ammonium, the concentration of total EPS increased from 264.9 to 231.1 mg/gVSS to 607.7 and 538.2 mg/gVSS, respectively, under the exposure to SDZ, which was mainly contributed by the increase of proteins in tightly bound extracellular polymeric substances (EPS) and polysacharides in tightly bound EPS and soluble microbial products. The proportion of tryptophan-like protein and humic acid-like organics in EPS also increased. Moreover, SDZ stress stimulated the secretion of three quorum sensing signal molecules, C4-HSL (from 140.3 to 164.9 ng/L), 3OC6-HSL (from 17.8 to 42.4 ng/L) and C8-HSL (from 35.8 to 95.9 ng/L) in the enriched AOB sludge. Among them, C8-HSL may be a key signal molecule that promoted the secretion of EPS. The findings of this study could shed more light on the cometabolic degradation of antibiotics by AOB.
研究领域:
环境微生物学
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生物降解与生物转化
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抗生素污染
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PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 19
·56108-56120
·
IF 5.8
Zhou Changrui,Gao Yun,Ma Qiang,Xia Zhuqing,Zhu Mengmeng,Zhang Xinhui,An Siyu,Li Shuailin,Yu Wantai
The coexistence of antibiotics and heavy metals in soil has attracted increasing attention due to their negative effects on microorganisms. However, how antibiotics and heavy metals affect functional microorganisms related to nitrogen cycle remains unclear. The goals of this work were to explore the individual and combined effects of sulfamethazine (SMT) and cadmium (Cd), selected as target pollutants in soil, on potential nitrification rates (PNR) and ammonia oxidizers (ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB)) structure and diversity by 56-day cultivation experiment. Results showed that PNR in Cd- or SMT-treated soil decreased at the beginning of the experiment and then increased over time. PNR was significantly correlated with AOA and AOB- amoA relative abundance ( P < 0.01). SMT addition (10 and 100 mg kg −1 ) significantly improved AOA activity by 13.93% and 17.93%, respectively, and had no effect on AOB at day 1. Conversely, Cd at 10 mg kg −1 significantly inhibited AOA and AOB by 34.34% and 37.39%, respectively. Moreover, the relative abundance of AOA and AOB in combined SMT and Cd addition clearly higher relative to single Cd at 1 day. The single and combined Cd and SMT increased and reduced the community richness of AOA and AOB, respectively, but reduced the diversity of both after 56 days. Cd and SMT treatments significantly changed the relative abundance of AOA phylum levels and AOB genus levels in the soil. It was mainly manifested in reducing the relative abundance of AOA Thaumarchaeota , and increasing the relative abundance of AOB Nitrosospira. Besides, AOB Nitrosospira was more tolerant to the compound addition of both than single application. Graphical abstract
研究领域:
环境科学
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微生物学
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土壤学
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PubMed
高影响因子
SENSORS
·
2018
·Vol. 18
·期 1
·105
·
IF 2.475
Hongyan Zhang,Jie Lv,Zhenhong Jia
We successfully demonstrate a porous silicon (PS) double Bragg mirror by electrochemical etching at room temperature as a deoxyribonucleic acid (DNA) label-free biosensor for detecting ammonia-oxidizing bacteria (AOB). Compared to various other one-dimension photonic crystal configurations of PS, the double Bragg mirror structure is quite easy to prepare and exhibits interesting optical properties. The width of high reflectivity stop band of the PS double Bragg mirror is about 761 nm with a sharp and deep resonance peak at 1328 nm in the reflectance spectrum, which gives a high sensitivity and distinguishability for sensing performance. The detection sensitivity of such a double Bragg mirror structure is illustrated through the investigation of AOB DNA hybridization in the PS pores. The redshifts of the reflectance spectra show a good linear relationship with both complete complementary and partial complementary DNA. The lowest detection limit for complete complementary DNA is 27.1 nM and the detection limit of the biosensor for partial complementary DNA is 35.0 nM, which provides the feasibility and effectiveness for the detection of AOB in a real environment. The PS double Bragg mirror structure is attractive for widespread biosensing applications and provides great potential for the development of optical applications.
研究领域:
光学传感
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环境微生物学
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生物传感器
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纳米材料
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分子诊断
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DOI
高影响因子
BIOLOGY AND FERTILITY OF SOILS
·
2021
·Vol. 57
·期 3
·399-407
·
IF 6.432
Li Yaying,Zhang Yang,Chapman Stephen James,Yao Huaiying
Sorghum has a great capacity to release biological nitrification inhibitors (BNIs), but the inhibitory effect on nitrification and ammonia oxidizer populations under planted soil conditions is unclear. A pot experiment with three nitrogen (N) application rates (0, 50, and 200 mg N kg −1 ) was set up to detect the influence of sorghum growth on soil nitrification and investigate the function of blocking the activity of ammonia oxidizers. A 15 N-labeled experiment was also conducted to detect the N form absorbed by sorghum. Sorghum root exudates were collected at 30 days after transplanting to hydroponic culture and added into cultured soil to determine the shifts in the populations of nitrifiers. The 15 N labeling experiment showed that the uptake rate by sorghum of ammonium N fertilizer was 24% and that of nitrate N fertilizer was 9%, indicating that sorghum was an ammonium using plant. Compared with unplanted soil, sorghum planting had a significant inhibitory effect on the nitrification process even at the high-N fertilizer rates. Autotrophic nitrification was the prevailing process, and sorghum root exudation inhibited this process as much as dicyandiamide (DCD, 10 mg kg −1 ). Root exudates had a significant inhibitory effect on ammonia-oxidizing bacteria (AOB) but had no effect on ammonia-oxidizing archaea (AOA).
研究领域:
土壤微生物学
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植物生理学
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环境科学
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农学
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DOI
高影响因子
ENVIRONMENTAL TECHNOLOGY
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2023
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IF 2.8
Ming Li,Yongqing Luan,Zhe Qin,Da Lu
In order to solve the complicated control of dissolved oxygen (DO) for partial nitrification in bioreactors treating high NH4+−N wastewater, a large height-diameter ratio anammox pre-reactor system was developed. And in this reactor, NO2−−N accumulation rate can reach 85.76% by alternate feeding with high NH4+−N wastewater (150 mg NH4+−N/L) and low NH4+−N wastewater (50 mg c) with low DO (0.19 mg/L–0.62 mg/L). Based on 16S rRNA identification technology, it was found that Nitrosomonas had a significant effect on NH4+−N oxidization in this study. And when the reactor treated higher concentration wastewater (250 mg NH4+−N/L), the growth rate of Nitrosomonas was higher than that of Nitrospira (nitrite-oxidizing bacteria, NOB), which was conducive to improving the NO2−−N accumulation rate and realizing partial nitrification stably. It was also found that the material exchange frequency of the microbial flora during alternate feeding with different NH4+−N concentration wastewaters was higher than that during feeding with higher NH4+−N concentration wastewater (250 mg/L) by Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolism pathways analysis. This study can provide valuable insights and lay the foundation for building anammox pre-reactors.
研究领域:
环境工程
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微生物学
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PubMed
高影响因子
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