PHYSICAL CHEMISTRY CHEMICAL PHYSICS
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2017
·Vol. 19
·期 33
·22472-22481
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IF 4.123
Jiying Liang,Xue Yu,Tiangang Yang,Menglu Li,Li Shen,Yue Jin,Hongyun Liu
In this paper, poly(N-isopropylacrylamide-co-3-aminophenylboronic acid) (P(NIPAM-co-APBA)) copolymer films were successfully electropolymerized on the Au electrode surface. The electroactive probe ferrocene carboxylic acid (FCA) in solution showed reversible thermal-, glucose- and pH-responsive on–off cyclic voltammetric (CV) behaviors at the film electrodes. The comparative experiments demonstrated that the thermo-responsive property of the film electrode was ascribed to the PNIPAM component of the films, whereas the glucose- and pH-sensitive behaviors came from the PAPBA constituent. The reduced form of nicotinamide adenine dinucleotide (NADH) could be electrocatalytically oxidized by FCA at the film electrodes, which would greatly amplify the multi-responsive CV signal difference between the on and off states. On the basis of these results, a binary 4-input/4-output logic circuit was fabricated with temperature, glucose, pH and NADH as inputs and the CV responses at 4 different levels as outputs. Moreover, a ternary CONSENSUS logic circuit was established on the same platform, which was the first report on the combination of ternary logic gate and bioelectrocatalysis without using enzymes. This work provided a novel idea for constructing complicated biocomputing systems by increasing the number of inputs/outputs with multi-sensitive interfaces and by designing new types of multi-valued logic gates on the basis of bioelectrocatalysis.
研究领域:
生物计算系统
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生物电化学
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PubMed
高影响因子
RSC Advances
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2018
·Vol. 8
·期 13
·6887-6892
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IF 2.936
Shanshan Li,Dongyan Li,Long Sun,Yuewei Yao,Cheng Yao
We fabricated a novel aminophenylboronic acid functionalized magnetic Fe3O4/zeolitic imidazolate framework-8/APBA (denoted as Fe3O4/ZIF-8/APBA). First, Fe3O4 was coated by zeolitic imidazolate framework-8 (denoted as Fe3O4/ZIF-8) using the hydrothermal method. Next, the phenylboronic acid functionalized triethoxysilane reagent was synthesized by 3-aminophenylboronic acid and 3-isocyanatopropyltriethoxysilane, which was modified on the surface of the Fe3O4/ZIF-8 nanocomposite through the sol–gel technique and electrostatic interaction as well as π–π stacking interaction. The synthetic Fe3O4/ZIF-8/APBA exhibited high adsorption capacity and good specificity toward glycoproteins. Moreover, the Fe3O4/ZIF-8/APBA possessed high saturation magnetization (51.41 emu g−1) and achieved better separation in the presence of an external magnetic field. Above all, the as-designed Fe3O4/ZIF-8/APBA was successfully used to capture glycoproteins and identify the horseradish peroxidase (HRP) tryptic digest. This study provides a facile strategy to embellish the aminophenylboronic acid onto the nanocomposite substrate and develop a new material for the specific recognition and enrichment of glycoproteins and low-abundance glycopeptides in proteomics research.
研究领域:
材料科学
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化学
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生物化学
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蛋白质组学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Chun Zhang,Xiaodong Jin,Liping Wang,Chengzhao Jin,Xiaoqian Han,Weigang Ma,Xingang Li,Guixiang Teng
Enrichment and detection of glycopeptides are an important clinical measure for the diagnosis of complex diseases. Enrichment materials play a key role in this process; they must have an effective sample-screening ability to eliminate the interference of nonglycopeptides. In this work, novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness were prepared for glycopeptide enrichment. The as-prepared composites have a suitable hollow structure and large specific surface area, and the boron hydroxyl group in their cavities can fix or disconnect the hydrophilic groups of the glycopeptides at different pH, so the glycopeptides can be adsorbed or desorbed in a controllable way. Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL–1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g). In order to verify the application of materials in practice, the HMCAs were used for the analysis of complex samples and it was found that 474 glycopeptides were identified from 210 glycoproteins in three replicate analyses of 2 μL of human serum. The results showed that the HMCAs could be used as a promising enrichment material for glycopeptide characterization in MS-based glycoproteomics and related fields.
研究领域:
生物化学
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分析化学
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医学诊断
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2016
·Vol. 408
·期 29
·8317-8323
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IF 3.125
Yan Zequn,Xue Min,He Qian,Lu Wei,Meng Zihui,Yan Dan,Qiu Lili,Zhou Lijun,Yu Yingjie
A novel polymerized crystalline colloidal array (PCCA) sensing material for the detection of urine glucose was developed by embedding a two-dimensional (2-D) polystyrene crystalline colloidal array (CCA) in 3-acrylamidophenylboronic acid (3-APBA)-functionalized hydrogel. After adjusting the cross-linker concentration, this material showed significant sensitivity for glucose under lab conditions, the particle spacing of the PCCA changed from 917 to 824 nm (93 nm) within 3 min as the glucose concentration increased from 0 to 10 mM, and the structural color of the PCCA changed from red through orange, to green, and finally, to cyan. In further experiments, this material was used to semi-quantitatively detect glucose in 20 human urine (HU) samples. Compared with the traditional dry-chemistry method, which was applied widely in clinical diagnosis, the PCCA method was more accurate and cost-effective. Moreover, this method can efficiently avoid the errors induced by most of the urine-interfering elements like vitamin C and ketone body. With a homemade portable optical detector, this low-cost intelligent sensing material can provide a more convenient and efficient strategy for the urine glucose detection in clinical diagnosis and point-of-care monitoring.
研究领域:
生物医学工程
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材料科学
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医学检测技术
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DOI
PubMed
高影响因子
ANALYTICAL LETTERS
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2019
·
IF 1.248
Xiaoyan Wang,Weiyang Shen,Xiaolei Zhang,Siyan Guo,Ye Gao,Xiaotong Li,Fang Feng,Gongjun Yang
A novel method for the indirect electrochemical determination of ribavirin based on boronic acid-diol recognition was developed using the platform as the sensing element. The device was constructed using a 3-aminophenylboronic acid (APBA)-electrochemically reduced graphene oxide (ERGO) modified electrode. When the electrode was immersed in a solution of ribavirin, complexation of boronic acid groups of APBA with ribavirin occurred at the surface of the electrode and simultaneously caused steric hindrance, resulting in a current decrease because the ferricyanide redox probe was unable access the surface. Under the optimized conditions, a linear relationship was obtained between the relative change in current (%Δi) of [Fe(CN)6]3−/4−and the concentration of ribavirin at levels from 10.0 to 7.50 × 102 ng mL−1. The proposed electrochemical sensor performed with acceptable sensitivity and reproducibility and was successfully used to determine the content of ribavirin in an injection with satisfactory results.
研究领域:
分析化学
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药物分析
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生物医学工程
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2022
·
IF 16.744
Shucheng Liu,Ying Sun,Danzhao Guo,Ruiheng Lu,Yuying Mao,Hongxiang Ou
For porous molecular imprinted polymers (MIPs), introduction of special functional groups and integration porous structure support will endow the MIPs with tunable surface properties and specific affinity performance. Herein, by combination of RAFT molecular imprinting, Pickering emulsion, and boronate affinity methods, a new kind of porous imprinted microspheres (BA-HPMIPs) using the low pKa value of APBA (1-allylpyridinium-3-boronic acid, pKa = 7.4) as functional monomer was prepared for specific molecular recognition and separation of NRG. Meanwhile, the novel RAFT functioned surfactants with dual amide multiple H-bonds were designed as stabilized particles was used to promote sustainability, which further confer them multifunctionality and/or by polymerizing the Pickering emulsion itself. Equilibrium adsorption was reached rapidly within 360 min, and the maximum equilibrium binding capacities of BA-HPMIPs were found to be 118.32 µmol g −1 for NRG. Furthermore, they had a stronger selectivity towards NRG than other competitive target (i.e rutin, catechol, ARS, o -nitrophenol). Moreover, BA-HPMIPs have shown outstanding reusability of 8.63 % loss in enrichment performance after five times repeatedly. As a proof of this concept, a commercially avaliable NRG with purity could be effectively extracted and concentrated to 96.75 % purity. These results demonstrated that, a new kind of porous imprinted microsphere with abundant porous structure and boronic acid recognizing sites has great potential for separation and purification of industrial samples under neutral conditions.
研究领域:
材料科学
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化学工程
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分析化学
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生物技术
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产品关联
DOI
高影响因子
MOLECULAR PHARMACEUTICS
·
2023
·
IF 4.9
Qingfa Tang,Yao Chen,Yusheng Zhang,Kewei Wang,Li Liu,Dongmei Pan,Qiang Liu,Cuiping Jiang
Tanshinone IIA (TS-IIA) and salvianic acid A (SAA) are the main pharmacological active constituents of Danshen, which exhibit potent effects on atherosclerosis. A combination of TS-IIA and SAA might exert a synergistic antiatherosclerotic effect. However, the opposite solubility profiles of TS-IIA and SAA might lead to difficulty in achieving a synergistic combined effect of the two active components. Therefore, in this work, we fabricated a ROS-responsive prodrug micelle for the codelivery of TS-IIA and SAA (TS-IIA-PM) by self-assembling amphiphilic block copolymer PEG5000-SAA/PLA10000-APBA. The amphiphilic polymer was characterized by 1H NMR, FTIR, and alizarin red S competition tests. The ROS responsiveness of TS-IIA-PM was evidenced by time-course monitoring of particle size and morphology changes and drug release behavior in the presence of 1 mM H2O2. We found TS-IIA-PM was stable according to its critical micelle concentration and the unchanged particle sizes in 10% FBS for 7 days. The results of in vitro and in vivo tests revealed that TS-IIA-PM was safe and biocompatible. Furthermore, it was observed that TS-IIA and prodrug micelle could produce synergistic antiatherosclerotic effect based on the results of the antioxidant study, which was further confirmed by a series of pharmocodynamics studies, such as in vitro DiI-oxLDL uptake study, oil red O staining, cholesterol efflux study, inflammatory cytokine analysis, in vivo CD68 immunostaining, and lipid disposition staining studies. Collectively, TS-IIA-PM holds great potential for the safe and efficient codelivery of TS-IIA and SAA for synergistic antiatherosclerosis.
研究领域:
药学
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纳米技术
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心血管疾病
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药物递送系统
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 73
·45978-45986
·
IF 3.108
Na Wen,Chunmei Gao,Shaoyu Lü,Xiubin Xu,Xiao Bai,Can Wu,Piao Ning,Shaofei Zhang,Mingzhu Liu
The high pKa (8.26 to 8.6) of PBA has restricted its glucose-responsiveness in physiological conditions, and the high cytotoxicity of polymers is also a limiting problem in their potential application for insulin delivery. Novel amphiphilic glucose-sensitive dialdehyde starch polymers containing 3-aminophenylboronic acid (APBA) as a glucose-responsive group and mPEGylated dialdehyde starch (mPEG-DAS) with hydrophobic 7-hydroxycoumarin-4-acetic acid (Cou) were synthesized. This dialdehyde starch derivative can self-assemble into mPEG-DAS–APBA–Cou micelles with “shell–core” structures in phosphate-buffered saline solution (PBS). In addition, the drug-loaded micelles can release insulin rapidly in response to hyperglycemia in a physiological environment. The results demonstrated that the mPEG-DAS–APBA–Cou micelles showed notable glucose responsive behavior near the physiological range. The insulin release from the nanocarriers is sensitive to different concentrations of glucose, releasing insulin rapidly under the conditions of 3 mg mL−1 glucose while demonstrating comparatively inert release at 1 mg mL−1 glucose (pH 7.4). MTT assays and hemolysis studies both confirmed that the mPEG-DAS–APBA–Cou micelles have low cytotoxic activity to A549 cells and low blood toxicity. These results suggest that the glucose-sensitive dialdehyde starch micelles (mPEG-DAS–APBA–Cou) have potential applications as a glucose-responsive material for insulin delivery.
研究领域:
药学
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纳米技术
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生物材料
·
药物递送系统
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）