Theranostics
·
2023
·
IF 12.4
Mingrui Xiong,Hong Chen,Yu Fan,Muchuan Jin,Dong Yang,Yuchen Chen,Yu Zhang,Robert B. Petersen,Hua Su,Anlin Peng,Congyi Wang,Ling Zheng,Kun Huang
Rationale: Ischemia-reperfusion injury (I/R) is a common cause of acute kidney injury (AKI). Post-ischemic recovery of renal blood supply plays an important role in attenuating injury. Exogenous application of elabela (ELA) peptides has been demonstrated by us and others to alleviate AKI, partly through its receptor APJ. However, the endogenous role of ELA in renal I/R remains unclear. Methods: Renal tubule specific ELA knockout ( Apela Ksp KO) mice challenged with bilateral or unilateral I/R were used to investigate the role of endogenous ELA in renal I/R. RNA-sequencing analysis was performed to unbiasedly investigate altered genes in kidneys of Apela Ksp KO mice. Injured mice were treated with ELA32 peptide, Nω-hydroxy-nor-L-arginine (nor-NOHA), prostaglandin E2 (PGE2), Paricalcitol, ML221 or respective vehicles, individually or in combination. Results: ELA is mostly expressed in renal tubules. Aggravated pathological injury and further reduction of renal microvascular blood flow were observed in Apela Ksp KO mice during AKI and the following transition to chronic kidney disease (AKI-CKD). RNA-seq analysis suggested that two blood flow regulators, arginine metabolizing enzyme arginase 2 (ARG2) and PGE2 metabolizing enzyme carbonyl reductases 1 and 3 (CBR1/3), were altered in injured Apela Ksp KO mice. Notably, combination application of an ARG2 inhibitor nor-NOHA, and Paricalcitol, a clinically used activator for PGE2 synthesis, alleviated injury-induced AKI/AKI-CKD stages and eliminated the worst outcomes observed in Apela Ksp KO mice. Moreover, while the APJ inhibitor ML221 blocked the beneficial effects of ELA32 peptide on AKI, it showed no effect on combination treatment of nor-NOHA and Paricalcitol. Conclusions: An endogenous tubular ELA-APJ axis regulates renal microvascular blood flow that plays a pivotal role in I/R-induced AKI. Furthermore, improving renal blood flow by inhibiting ARG2 and activating PGE2 is an effective treatment for AKI and prevents the subsequent AKI-CKD transition.
研究领域:
肾病学
·
药理学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
Cell Communication and Signaling
·
2025
·Vol. 23
·期 1
·1-17
·
IF 8.9
Song Ke,Jing Tao,Yang Xinyan,Xia Junjun,Wang Zehui,Luo Fang,Qiu Chunhua,Liu Zhaoting,Zhao Xiao-Yang,Wang Guang,Ou Xianghong
Type 2 diabetes mellitus (T2DM) is frequently associated with testosterone deficiency, which affects male reproductive function. Currently, exogenous testosterone replacement therapy poses inherent risks, underscoring the need to develop safer and more effective treatment strategies. This study aimed to elucidate the mechanism by which ML221, a potent apelin receptor (APJ) functional antagonist, promotes testosterone secretion, and alleviates reproductive dysfunction. Diabetic mouse models demonstrated reduced testosterone levels, impaired spermatogenesis, and low sperm quality. Elevated APLN expression impaired Leydig cell function and testosterone synthesis. Treatment with ML221 restored testosterone levels and spermatogenesis in diabetic mice. Mechanistically, ML221 regulated Leydig cell metabolism by elevating S-D-lactoylglutathione levels, reducing mitochondrial reactive oxygen species, preserving mitochondrial membrane potential, and enhancing adenosine triphosphate production. At the epigenetic level, ML221 enhanced the binding of nuclear receptor subfamily 2 group F member 2 to the promoters of testosterone-synthesizing genes, thereby facilitating testosterone biosynthesis. These findings advance the understanding of the male reproductive system in the context of diabetes and highlight ML221 as a potential therapeutic approach for T2DM-induced testosterone deficiency.
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DOI
PubMed
高影响因子
AQUACULTURE RESEARCH
·
2020
·Vol. 51
·期 3
·1012-1025
·
IF 1.748
Xiao Yan,Chaobin Qin,Dapeng Deng,Guokun Yang,Junchang Feng,Ronghua Lu,Liping Yang,Xiaolin Meng,Xianglin Cao,Guoxing Nie,Guiqin Wang
Apelin and its receptor apj have a variety of biological functions and are involved in appetite and energy regulation. However, the comparative studies of apelin/apj system in fish are still absent. In this study, the open reading frames of apelin and apja and apjb were obtained. Apelin and apj mRNAs were widely distributed in all tissues detected, with the highest level in the brain, followed by the spleen, kidney and foregut. The mRNA expression of apelin and apj s increased rapidly at mealtime and significantly decreased after 30 min of feeding. In addition, there were obvious circadian rhythms (higher at nighttime and lower at daytime) in the expression of apelin and apja and apjb . Furthermore, fasting elevated the mRNA levels of apelin and apj s in the hypothalamus, foregut and hepatopancreas. When the 8-day fasted fish were refed, apelin and apj s mRNA expression significantly decreased. However, there were differences in the response to fasting and refeeding between apja and apjb . The roles of apelin and its receptor apjs in fish are poorly understood. Herein, we employed molecular and physiological studies to characterize their structures, distribution and mRNA expression responses to periprandial changes and fasting–refeeding in Cyprinus carpio L. for the first time.
研究领域:
分子生物学
·
生理学
·
水产养殖
·
营养学
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产品关联
DOI
高影响因子
Asia-Pacific Journal of Chemical Engineering
·
2022
·Vol. 18
·期 1
·e2841
·
IF 1.777
Chunxiang Ma,Xiaoning Wang,Yijun Song,Haoyu Hu,Wei Li,Zhijian Qiu,Yongpeng Cui,Wei Xing
LiFePO 4 is one of the most widely used cathode materials for lithium-ion batteries, and the low-temperature performance of LiFePO 4 -based batteries has been widely studied in recent years. Herein, a 3.5 Ah pouch-type full battery was assembled using LiFePO 4 as the cathode and artificial graphite as the anode. For the LiFePO 4 -based cathode, carbon-coated aluminum foil was selected as the current collector, and a 0D–1D–2D multi-scale conductive network was introduced into the electrode. Notably, both the carbon-coated current collector and novel conductive network could significantly reduce the internal resistance of the LiFePO 4 -based battery, thus improving its electrochemical dynamics and low-temperature performance. This paper provides a theoretical basis for the development of low-temperature LiFePO 4 -based batteries.
研究领域:
材料科学
·
电化学
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DOI
高影响因子
Asia-Pacific Journal of Chemical Engineering
·
2023
·Vol. 18
·期 4
·e2908
·
IF 1.8
Tao Ge,Yuqing Wang,Jingyao Xu,Dinghao Liu,Yulu Wei,Zhaohui Wang
The microwave absorption properties of dielectrics determine the promotion degree of microwave energy to chemical reaction. The wave-absorbing characteristics of organic sulfur compounds in high-sulfur coking coal need to be studied to improve the efficiency of microwave desulfurization of coal. In this study, the imaginary part of complex dielectric constants ( ε′′ ) and dielectric loss tangent (tan σ ) of benzene mercaptan, difurfuryl disulfide, and benzothiophene were characterized. The dipole moment, molecular configuration, and frontier molecular orbital energy of organic sulfur compounds were simulated and calculated under the different external energy fields. The migration law of mercaptan, sulfide, and thiophene in coal in microwave field were analyzed. The results show that the ε'′ and tan σ of benzothiophene slightly changed with the change in microwave frequency in the frequency range of 500–2500 MHz, and the microwave absorption properties are weaker than those of benzene mercaptan and difurfuryl disulfide. Therefore, difurfuryl disulfide and benzene mercaptan molecules are more active in the microwave field than benzothiophene. The sulfur components in benzene mercaptan and difurfuryl disulfide can dissociate in the form of small inorganic sulfur molecules of carbon sulfides and disulfides, respectively. After microwave irradiation, the relative content of mercaptan and thioether in coal was decreased, whereas the relative content of thiophene remained almost unchanged.
研究领域:
能源化学
·
量子化学
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DOI
高影响因子
Asia-Pacific Journal of Chemical Engineering
·
2016
·Vol. 11
·期 5
·673-682
·
IF 0.728
Rui Li,Zhaoliang Wu,Yanji Wang,Linlin Ding,Wei Liu
Prevention of irreversible aggregation of whey soy proteins (WSP) in their foam fractionation from soy whey wastewater is important for achieving their large-scale recovery. For effectively preventing the irreversible aggregation of WSP, this work studied its dependencies on the WSP enrichment ratio and the height of the collected foam which freely collapsed. The results show that most insoluble aggregates formed in the desorption of WSP from the gas–liquid interface, and their formation was closely related to their high local enrichment in the foam. Based on the above results, a chemical defoaming agent with 25% Tween 20 was developed to reduce the relative content of insoluble aggregates in the foamate and the decrease reached about 80%, compared to the case without Tween 20. The prevented irreversible aggregation effectively improved food-related properties of the WSP product. Copyright © 2016 Curtin University of Technology and John Wiley & Sons, Ltd.
研究领域:
食品科学
·
蛋白质工程
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DOI
高影响因子
Asia-Pacific Journal of Chemical Engineering
·
2020
·Vol. 16
·期 1
·e2581
·
IF 1.06
Tao Ge,Fen Li,Yang Li
The present study aims to reveal the influence mechanism of organic sulfur structures and microwave frequency on its dielectric property and establish the relationship between the dielectric properties of organic sulfur components in coal and the efficiency of microwave desulfurization. Results showed that the different polarization mechanisms of organic sulfur compounds lead to the real part of complex permittivity decreased initially and then increased with increasing frequency. At the same time, the real parts of the complex permittivity of mercaptan and thioether compounds were higher than that of thiophene, which were caused by their different structures. As the frequency increased, the loss tangent, microwave penetration depth, and absorption index showed decline in oscillation. The loss tangent was mainly related to the polarization mechanism; both polarization mechanism and microwave wavelength had effect on the changes of microwave penetration depth and absorption index. Mercaptan and thioether compounds were more effective at converting microwave energy into heat energy. After microwave processing, the relative content of mercaptan (thioether) in GZ, CQ, and SX coals decreased from 37.38%, 36.93%, and 40.02% to 27.34%, 29.26%, and 26.67% and of thiophene increased from 47.61%, 53.35%, and 49.54% to 58.76%, 59.09%, and 60.38%.
研究领域:
能源科学
·
化学工程
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DOI
高影响因子
Asia-Pacific Journal of Chemical Engineering
·
2024
·
IF 1.4
Ze Cheng,Fuxiao Zhu,Bihui Jin,Zhenhua Hou,Hongbin Li,Gongliang Zhang,Hongman Hou,Jingran Bi,Shuang Yan,Hongshun Hao
Photocatalytic technology has been widely studied, discussed, and tested as a feasible wastewater degradation technology. The present study demonstrated the preparation of g-C 3 N 4 material using solid-state sintering method and the construction of Bi 4 Ti 3 O 12 /g-C 3 N 4 heterostructure via hydrothermal method. This direct contact Z-type heterojunction improves the wide bandgap of Bi 4 Ti 3 O 12 monomer, enhances its photoresponsiveness, and thus improves its photocatalytic performance. During the process of photocatalytic experiments, an environment of cold and hot fluctuations were created, and the pyroelectric effect was utilized to induce self-polarization of the material and formed an internal electric field. It improves the transport ability of electron–hole pairs and suppresses their recombination in the transport path. According to the degradation experiment results, the degradation efficiency of pyroelectric synergistic heterojunction photocatalysis reached 88.7%, which was 2.27 times that of Bi 4 Ti 3 O 12 . It proves that the photocatalysis by pyroelectric synergistic heterojunction has good application prospects.
研究领域:
材料科学
·
环境科学
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产品关联
DOI
高影响因子
Asia-Pacific Journal of Chemical Engineering
·
2024
·Vol. 19
·期 4
·e3076
·
IF 1.4
Haoran Zhao,Yu Jia,Yihang Chen,Xuanyu Liang,Jinbo Hao,Binglin Chen,Chao He,Liang Liu,Chun Chang,Guizhuan Xu
Ethyl levulinate (EL) production from steam-exploded corn straw (SCS) in a cascade of reaction using a Brønsted (B) acid and a Lewis (L) acid in ethanol was studied. The entangled structure of corn straw could be obviously damaged through steam explosion when the pressure was 1.5 MPa holding 10 min. The content of cellulose can be increased from 35.9% to 46.8%, and the contents of hemicellulose and lignin were changed from 16.7% to 8.8% and 22.6% to 27.5%, respectively. EL yield was significantly increased from 10.7 to 24.6 wt% under optimal reaction conditions (L/B = 1/20 [mol/mol], 205°C, 90 min, 1.8 g of SCS, 60 mL of ethanol). According to kinetic models, the activation energies for the main and side reactions were 56.8 and 110.5 kJ mol −1 , respectively. It suggested that SCS was more easily to be converted to EL rather than other by-products. The highest occupied molecular orbital (HOMO)–lowest unoccupied molecular orbital (LUMO) energy gaps (HOMO-LUMO gaps) of cellobiose over the mixed acids in ethanol were significantly reduced with frontier molecular orbital (FMO) theory. This work provides an effective strategy for EL production from agricultural waste straws.
研究领域:
生物化学
·
可再生资源与环境科学
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产品关联
DOI
高影响因子
Asia-Pacific Journal of Chemical Engineering
·
2024
·
IF 1.4
Guihua Yang,Jiali Chen,Linxue Yang,Rui Wang
Cu-based catalysts applied in ethynylation reaction of formaldehyde for 1,4-butynediol synthesis has been widely concerned. The activity and stability of Cu-based catalyst is still a challenging task in this field. Here, Cu–Zn catalysts derived from ZIF-8 are prepared by a coprecipitation method and applied in ethynylation reaction of formaldehyde. All catalysts were characterized through thermogravimetric, x-ray diffraction, N 2 physical adsorption–desorption, transmission electron microscopy, H 2 -temperture-programmed reduction, x-ray photoelectron spectroscopy, and Raman and Fourier transform infrared analysis. The effect of calcination temperature of ZIF-8 on the catalyst structures and ethynylation performances are all investigated. The results show that CuO 5h -ZnO 400 catalyst has the best catalytic activity, with a formaldehyde conversion of 98% and 1,4-butynediol selectivity of 100%. It is mainly due to the presence of highly dispersed and small particle CuO. Moreover, CuO 3h -ZnO 400 catalyst prepared by optimized conditions can further improve the stability in ethynylation reaction due to more carbon species on the surface of ZnO. The more carbon contents in Cu–Zn catalyst contribute to the ethynylation activity and stability due to the interaction between Cu and C species favoring Cu 2 C 2 formed. In addition, the ethynylation reaction mechanism catalyzed by Cu–Zn catalyst is illustrated carefully. The Cu–Zn catalysts derived from ZIF-8 can provide some ideas for the application in ethynylation reaction of formaldehyde for 1,4-butynediol synthesis.
研究领域:
化学工程
·
催化材料科学
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产品关联
DOI
高影响因子
Asia-Pacific Journal of Chemical Engineering
·
2024
·Vol. 19
·期 4
·e3074
·
IF 1.4
Fangqin Lu,Lingyun Liu,Chuilei Kong,Hongyu Zhao
Addressing the issue of processing fine kaolinite and quartz particles in coal slime, this study utilized molecular simulation and Density Functional Theory (DFT) to investigate the chelate adsorption characteristics of sodium dodecyl sulfate (SDS) on kaolinite surfaces. As a major clay mineral component in coal slime, kaolinite reduces coal's calorific value but holds potential for industrial and agricultural applications. The research identified distinct interactions between SDS and the tetrahedral Si O layer and octahedral Al O layer of kaolinite, in contrast to quartz, which contains only the tetrahedral Si O layer. This difference is crucial for the effective separation of kaolinite from quartz. The study focused on analyzing SDS adsorption on the (001) and (00-1) planes of kaolinite. The findings revealed strong adsorption of SDS on kaolinite surfaces, especially on the (001) plane, evidenced by significant charge transfer indicating efficient chelation. This effect results from the interaction of SDS's electron-donating atoms (such as S and O) with the metal atoms on the surface of kaolinite. Adsorption strength was quantified through adsorption energy calculations, showing a stronger interaction on the (001) surface. Experimental validations, including single mineral flotation experiments and infrared spectroscopic analysis, further corroborated the simulation outcomes. These tests demonstrated improved flotation recovery of kaolinite in the presence of SDS and with reduced particle size. Infrared analysis revealed that SDS selectively and strongly adsorbs on kaolinite surfaces, as indicated by diminished hydroxyl group stretching vibrations in the FTIR spectrum and changes in absorption peaks related to inorganic vibrations and sulfonic acid groups. The study demonstrates that SDS can selectively and effectively adsorb onto kaolinite surfaces, particularly on the (001) plane, facilitating the efficient extraction of fine kaolinite from coal slime. This research holds significant potential for enhancing the utilization of resources from coal slime in the coal industry, offering both economic and environmental benefits.
研究领域:
矿物加工工程
·
表面化学
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产品关联
DOI
高影响因子
Asia-Pacific Journal of Chemical Engineering
·
2024
·
IF 1.4
Jianke Tang,Qiaoling Li,Rongqian Meng,Yanfeng Xue,Shengjian Zhang,Shengjuan Shao
Using the prepared Ag 3 PO 4 /WO 3 ·H 2 O composite as the matrix, AgI nanoparticles were introduced onto the surface of Ag 3 PO 4 to construct AgI/Ag 3 PO 4 /WO 3 ·H 2 O ternary heterojunction photocatalysts. The ternary composite photocatalysts exhibited higher photocatalytic degradation activities for rhodamine B (RhB) and tetracycline hydrochloride (TCH) than those of binary Ag 3 PO 4 /WO 3 ·H 2 O and AgI/WO 3 ·H 2 O photocatalysts and individual AgI, Ag 3 PO 4 , and WO 3 ·H 2 O photocatalysts. After exposure to visible light for 12 and 24 min, the removal efficiencies of RhB and TCH by the S2-50% photocatalyst (50% molar percentage of AgI to Ag 3 PO 4 ) reached 98.0% and 85.3%, respectively. After four degradation cycles, the removal efficiency of RhB by S2-50% remained at 93.2%, significantly surpassing that of the Ag 3 PO 4 /WO 3 ·H 2 O photocatalyst. The enhanced photocatalytic performance of the AgI/Ag 3 PO 4 /WO 3 ·H 2 O photocatalyst is primarily attributed to the formation of Z-scheme/conventional Type II heterojunctions, which facilitate spatial separation of the photogenerated electrons and holes while maintaining the strong reductive capabilities of electrons in the AgI conduction band.
研究领域:
材料科学
·
环境科学
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产品关联
DOI
高影响因子
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