International Journal of Environmental Research and Public Health
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2013
·Vol. 10
·期 7
·2897-2905
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IF 1.998
Juan Peng,Shanshan Song,Liguang Xu,Wei Ma,Liqiang Liu,Hua Kuang,Chuanlai Xu
We have established a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) based on two monoclonal antibodies (mAb) to measure the content of the major peanut allergen Ara h 1 in foods. Two mAbs were selected out of 12 murine hybridoma cells secreting Ara h 1-specific antibody. Using mAb 6 as the capture antibody and HRP-labelled mAb 4 as the detection antibody, the limit of detection (LOD) the assay was 0.34 ng/mL. Cross-reaction analysis showed that this method was strongly specific and had no cross-reactions with Ara h 2, pea protein or soy protein. Sample analysis showed that this ELISA was a useful tool to monitor peanut allergens in food products by measuring Ara h 1 content.Keywords:peanut allergen;Ara h 1;monoclonal antibody;sandwich ELISA
研究领域:
食品科学
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免疫学
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分析化学
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PubMed
高影响因子
Food Analytical Methods
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2015
·Vol. 8
·期 10
·2605-2611
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IF 1.956
Peng Juan,Song Shanshan,Liu Liqiang,Kuang Hua,Xu Chuanlai
In order to develop highly sensitive immunoassays for detecting the major peanut allergen, Ara h 2, we prepared six monoclonal antibodies (mAb) with high affinity to Ara h 2. Using mAb 5 as a capture antibody and HRP-labeled mAb 1 as a detection antibody, a sandwich enzyme-linked immunoassay (ELISA) was established with a limit of detection (LOD) of 0.02 ng/mL. An immunochromatographic strip based on these two mAbs was also established with a LOD of 1 ng/mL. Both tests showed high specificity in cross-reactivity experiments. Sample analyses indicated that these two immunoassays were feasible, accurate, and highly sensitive for measuring allergen Ara h 2 of food.
研究领域:
食品科学
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免疫学
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生物化学
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高影响因子
CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Shumin Zhang,Kexin Zou,Qingmin Chen,Jie Wei,Tianhui Jiao,Min Chen,Yi Xu,Quansheng Chen,Xiaomei Chen
Developing a fast, accurate, and simple detection method for food allergens is imperative to prevent food allergy. In this study, an on–off-on photoelectrochemical (PEC) aptasensor with dual-signal amplification was constructed based on a covalent organic frame (COF) film to detect β-lactoglobulin (BLG) and Ara h1. Specifically, a uniform and stable TFTA COF film was in situ grown as a PEC signifier on the surface of an indium tin oxide (ITO) electrode. BLG recognition triggered enzyme-assisted circular signal amplification to introduce non-conductive SiO 2 -modified DNA (S0) to the platform, effectively quenching the PEC signal for ultra-sensitive detection of BLG. Upon adding Ara h1, SiO 2 -DNA was released from the sensing platform to restore the PEC signal, and hemin was added to form a G-quadruplex/hemin compound for secondary signal amplification. Thus, sensitive detections of BLG and Ara h1 were achieved with low detection limits (18 ng/mL for BLG and 2.6 ng/mL for Ara h1) and wide linear ranges (0.05–5 µg/mL for BLG and 0.01–0.2 µg/mL for Ara h1). The dual-amplification and dual-target-responsive PEC aptasensor provides a new idea to develop PEC analysis methods for multi-targets in food and environmental monitoring.
研究领域:
食品科学
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生物医学工程
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分析化学
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2022
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IF 9.221
Shuo Qi,Anqi Sun,Xiaoze Dong,Yuhan Sun,Minwei Qin,Ning Ding,Yin Zhang,Zhouping Wang
Food allergy is a serious immune allergy reaction, that has attracted public attention all over the world due to the increasing incidence. Ara h1, the most abundant allergenic protein in peanuts, has been identified as a biomarker of peanut allergen. Therefore, a sensitive, efficient method for Ara h1 detection is urgently needed to protect allergy sufferers. In this study, a simple and efficient fluorescent aptasensor was proposed for ultrasensitive and rapid detection of peanut allergen Ara h1. The aptamer against Ara h1 was conjugated onto the surface of high-stable perovskite nanocrystal (PNCs) to serve as the fluorescence-labeled recognition probe as well as the signal probe , while the magnetic nanoparticles Fe 3 O 4 (MNPs) were functionalized with the aptamer complementary DNA (cDNA) as the separation probe. The high-stable PNCs-labeled aptasensor [email protected] gives the combined merits of the extraordinary optical properties of PNCs, the efficient magnetic separation capability of MNPs as well as the high specificity of aptamers. The developed high-stable PNCs-labeled aptasensor exhibited excellent linearity (0.1–100 ng/mL), and low limit of detection (0.04 ng/mL). The aptasensor was successfully applied for the determination of Ara h1 in food samples, implying great potential in the field of allergen assay.
研究领域:
食品科学
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分析化学
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生物技术
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高影响因子
FOOD CHEMISTRY
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2023
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IF 8.8
Ping Yang,Xumei Wang,Hui Wang,Yueming Hu,Pingwei Wen,Zongcai Tu
The mechanism of the effect of reducing sugar chain length and isomers on the allergenicity of Ara h 2 after glycation was investigated. Ara h 2 was more prone to glycation with ribose which had a short chain length. The glycation sites of Ara h 2 after glycation with galactose were higher than the glycation sites in galactose’s isomers-Ara h 2 conjugates, which might be affected by the configuration differences at position C-4 and the small steric effects in terminal groups –CHO of galactose. Ara h 2-ribose conjugate had the lowest allergenicity, and glycation with galactose was more capable of reducing Ara h 2 allergenicity than its isomers. The results indicated that glycation with ribose caused conformational epitope destruction and linear epitope masking of Ara h 2 greatly. Furthermore, since the small steric effects of –CHO, galactose was more capable of reducing Ara h 2 allergenicity than fructose. This study will provide a theoretical basis for selecting appropriate reducing sugars and preparing hypoallergenic products containing peanuts.
研究领域:
食品科学
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过敏学
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生物化学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2023
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IF 8.8
Chixuan Yao,Yaozhong Hu,Qisijing Liu,Jing-Min Liu,Xuemeng Ji,Huan Lv,Shuo Wang
To improve the performance of peanut allergen Ara h 3 detection, depending on boron and nitrogen carbon dots (B/ N -CDs), a nanobody (Nb) mediated dual-mode immunoassay was established, which combines the dominance of colorimetry with ratiometric fluorescence techniques. With the catalysis of Horseradish peroxidase (HRP), the oxidization of o-phenylenediamine (o-PD) in the presence of H 2 O 2 , leading to the production of yellow 2,3-diaminophenolazine (DAP) with an absorption peak at 431 nm. Owing to inner filter effect (IFE), DAP quenched the fluorescence of B/ N -CDs at 426 nm, and it emerged a new emission peak at 549 nm. The fluorescence intensity ratio and absorption intensity can be utilized for quantitative analysis of Ara h 3 concentration. Under optimal conditions, the detection limits were 6.61 and 9.79 ng·mL −1 , respectively. The dual-mode immunoassay was assessed containing specificity, stability, reproducibility, and practicability. This method paved the way for sensitive detection of Ara h 3 without background Interference.
研究领域:
过敏学
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分析化学
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纳米技术
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食品安全
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DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
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2025
·Vol. 90
·期 8
·e70432
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IF 3.4
Qin Geng,David Julian McClements,Taotao Dai,Wenlong Zhou,Zhihua Wu,Hongbing Chen
The molecular structure and surface properties of peanut proteins are altered by their non-covalent interactions with food polyphenols, which alter their allergenicity. In our research, the interactions between the critical peanut allergen (Ara h 2) and a model polyphenol (tannic acid [TA]) were studied utilizing experimental (spectroscopy and calorimetry) and molecular docking methods. Ultraviolet (UV) and circular dichroism (CD) spectroscopy analysis indicated that the microenvironment polarity and secondary/tertiary structure of Ara h 2 were changed due to its interaction with TA. Specifically, degree of molecular disorder increased, and the surface hydrophobicity of the polypeptide decreased after Ara h 2 interacted with TA. Analysis using fluorescence quenching, isothermal titration calorimetry, and molecular docking showed that the main non-covalent interactions were hydrogen bond formation between the Ara h 2 and TA. Binding site analysis using molecular docking indicated that the TA may have interacted with specific amino acids in the polypeptide chain, including Lys 31, Arg 34, Asp 35, Trp 44, Ser 54, Arg 59, Arg 60, Arg 122, Glu 123, and Gln 130. These amino acids are known to be part of the IgE epitope regions of Ara h 2, and so TA binding might be expected to impact peanut protein allergenicity. Indeed, an enzyme-linked immunosorbent assay (ELISA) study indicated that the IgE binding capacity of Ara h 2 was reduced after interacting with TA in a dose-dependent manner. This study enriches the understanding of the interactions between peanut protein allergens and small-molecule nutrients and provides a method for reducing the potential allergenicity of Ara h 2. Practical applications Employing the plant polyphenols as a means to modulate the activity of food allergens could potentially offer a breakthrough in managing food allergies. The strategic non-covalent binding of polyphenols, exemplified by TA, to the peanut allergen Ara h 2, may effectively lower its capacity to trigger allergic reactions, thereby offering an approach to mitigating peanut sensitization risks. The study examined the alterations in the structure and allergenic properties of Ara h 2 following its interaction with TA. This investigation was poised to accelerate the identification of TA's potential to inhibit peanut-induced allergic responses through allergen binding.
研究领域:
食品科学
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过敏研究
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分子生物学
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生物化学
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免疫学
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结构生物学
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PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2019
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IF 3.571
Hengqian Lu,Haiqin Chen,Xin Tang,Qin Yang,Hao Zhang,Yong Q. Chen,Wei Chen
The objective of the present study was to reveal the effects of four types of nitrogen sources (soymeal, yeast extract, KNO3, and ammonium tartrate) on the lipid metabolism of the oleaginous fungus Mortierella alpina using untargeted lipidomics, targeted fatty acid, and reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis. Our results showed clear differences in the contents and compositions of lipids between four types of nitrogen sources. Soymeal and ammonium tartrate supplementation favored the accumulation of triglycerides with arachidonic acid (ARA) and C16–18 fatty acids, respectively. These results were further validated by our targeted fatty acid analysis. RT-qPCR analysis of related genes in M. alpina between the four nitrogen source conditions found that soymeal supplementation dramatically increased the expression of GPAT, ELOVL, and Δ12/Δ6 desaturase. Our findings provided new insights into the regulation of lipid biosynthesis in M. alpina and potential avenues for genetic manipulation and highlighted the importance of an optimal nitrogen source for ARA-rich oil production.
研究领域:
生物化学
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微生物学
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分子生物学
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DOI
PubMed
高影响因子
Indian Journal of Hematology and Blood Transfusion
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2024
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IF 0.7
Liu Xiaoyu,Yang Lin,Guan Kunping,Chen Zhen,Yang Hua
Purpose This study aims to investigate the increased toxicity of Cytarabine (Ara-c) by knockdown of chromosome segregation 1-like (CSE1L) in acute myeloid leukemia(AML) cells(Kasumi-1, U937, and THP-1 cells) and to explore its possible mechanisms. Methods Target gene silencing was achieved using the shRNA-mediated lentivirus method. Apoptosis was identified using the Annexin V PE/7-AAD double-staining assay. Cell viability was assessed with the Cell Counting Kit-8 (CCK-8) assay. Protein expression was detected by Western blotting. Results In vitro, knocking down CSE1L promoted caspase-3 and caspase-9 proteins expression and induced apoptosis in AML cells. Knockdown of CSE1L enhanced AML cells' sensitivity to Ara-c. knockdown of CSE1L reduced the expression levels of p-JKA2 and p-STAT3 proteins, while no significant difference was observed in the expression levels of total JAK2 and STAT3 proteins. Furthermore, JAK2 overexpression reversed the increase in Ara-c toxicity to AML cells caused by CSE1L knockdown. Conclusion In conclusion, our study reveals that CSE1L is a potential therapeutic target for overcoming Ara-c resistance in AML cells. Thus, we have gained new insights into the oncogenic process of CSE1L in AML cells and raised the prospect of knockdown of CSE1L in AML in combination with cytarabine-targeted therapy.
研究领域:
肿瘤学
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血液学
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分子生物学
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细胞生物学
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药理学
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PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
·
IF 6.2
Kuan Gao,Qin Geng,Changbao Hu,Wenhui Zhang,Xin Li,Ping Tong,Anshu Yang,Zhihua Wu,Hongbing Chen
Interactions between food allergens and polyphenols can provide a method to alleviate food allergies. This study investigated the effect of differently structured polyphenols complexed with Ara h 2 based on high-throughput virtual screening, utilizing a spectroscopic, thermodynamic, bone marrow-derived dendritic cells (BMDCs) model, BMDCs-T cells coculture model, KU812 cells model, and molecular simulation analyses for the allergenicity evaluation. Hesperidin (HSD), neohesperidin (NH), and neohesperidin dihydrochalcone (NHDC) exhibited a high binding energy and interacted with Ara h 2 through hydrogen bonding, primarily at two additional binding sites. This interaction led to protein unfolding and reduced hydrophobicity, particularly in the NH–Ara h2 complex. In addition, NH-bound Ara h2 has a lower potential for sensitization due to its reduced immunoglobulin E (IgE) binding capacity compared with Ara h 2 during gastrointestinal digestion. This is further supported by the decreased expression of costimulatory factors in BMDCs, the reduced release of allergenic cytokines, and the maintained balance of Th1, Th2, Th17, and Treg. The same trend was followed by the HSD and NHDC complexes. These findings suggest that glycosidic bond positions play a more significant role in reducing sensitization than does the flavonoid structure. Therefore, this study can assist in developing oral-specific immunotherapy of individuals with peanut allergies under the potential interaction mechanism of polyphenols and Ara h 2.
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2026
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IF 6.2
Kuan Gao,Qin Geng,Wenhui Zhang,Xin Li,Ping Tong,Anshu Yang,Zhihua Wu,Hongbing Chen
The effects of pH-shifting combined with quercetin binding on peanut Ara h 2 were evaluated using multispectroscopy, cell models, molecular simulation, and correlation analysis. After dual treatment, Ara h 2 exhibited significant structural changes, as evidenced by decreased intrinsic fluorescence and converted α-helix into β-sheet, leading to reduced IgE-binding capacity and diminished ability of dendritic cells and T cells to differentiate toward the Th2 pathway due to the masking of epitopes. Moreover, the binding sites of quercetin gradually shifted toward the hydrophobic core of Ara h 2 with pH changes, which inhibited fluctuations in the aa50–70 region. Finally, according to the correlation analysis, pH-shifting combined with quercetin binding may reduce the allergenicity of Ara h 2 by the migration of many Trp and Tyr residues to the protein surface, which disrupted the conformational epitopes. Therefore, this study provides an approach for the development of hypoallergenic peanut products.
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高影响因子
Nanoscale
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2019
·Vol. 11
·期 47
·23000-23012
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IF 6.97
Zhigang Fang,Xiaozhen Wang,Yanling Sun,Ruifang Fan,Zhong Liu,Rui Guo,Deming Xie
Cytarabine (Ara-C) is an essential medicine used in the clinical treatment of acute lymphoblastic leukemia. However, Ara-C suffers from high hydrophilicity, rapid plasma degradation and significant side effects. Thus, herein, to eliminate the limitations of Ara-C in the treatment of leukemia, Sgc8 aptamer targeting and glutathione (GSH)-responsive polymeric micelles (PCL-ss-Ara@Sgc8-BSA) were prepared. The prodrug was synthesized via covalent bond formation between acryloyl chloride-terminal PCL-ss-PCL and Ara-C, and surface decoration with Sgc8-bovine serum albumin (Sgc8-BSA). The obtained PCL-ss-Ara@Sgc8-BSA exhibited good GSH-responsive drug release behavior, obvious targetability and sufficient antitumor effect to acute lymphoblastic leukemia (ALL) cells (CCRF-CEM). A hemolysis test was further carried out to demonstrate that these polymeric micelles are safe to be administrated intravenously. Compared with free Ara-C, PCL-ss-Ara@Sgc8-BSA significantly enhanced tumor growth inhibition in mice bearing CCRF-CEM xenograft tumors, while causing little side effects, and improved the survival rate of CCRF-CEM tumor-bearing mice in vivo. Therefore, this new self-assembling small molecular prodrug equipped with Sgc8 targeting function is a potential treatment for the targeted therapy of acute lymphoblastic leukemia.
研究领域:
药学
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纳米技术
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肿瘤学
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DOI
PubMed
高影响因子
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