Journal of Materials Chemistry B
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2018
·Vol. 6
·期 23
·3969-3978
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IF 4.776
Bin Luo,Xiaoxi Zhou,Peipei Jiang,Qiangying Yi,Fang Lan,Yao Wu
Highly effective enrichment of phosphorylated biomolecules is vital for an in depth and comprehensive phosphoproteome analysis. Among affinity materials, materials based on the immobilized metal affinity chromatography (IMAC) method are most commonly used in phosphopeptide enrichment. However, the problem of metal cation loss in IMAC materials is still a challenge. In this study, a novel strategy has been developed for both highly effective enrichment of phosphorylated biomolecules and prevention of metal cation loss using Fe3O4/polydopamine/poly(2-aminoethyl methacrylate hydrochloride)/arginine (Fe3O4/PDA/PAMA–Arg) composite nanospheres. The magnetic composite nanospheres were prepared by a facile method, possessing uniform morphology, a high saturation magnetization (45 emu g−1), and a quick magnetic response (within 10 s). Noticeably, abundant guanidyl and amino groups of the PAMA–Arg brushes endowed the nanospheres with extremely high detection sensitivity (0.2 fmol), excellent selectivity (β-casein/BSA = 1 : 500), and high recyclability (5 cycles) in phosphopeptide analysis. Furthermore, the Fe3O4/PDA/PAMA–Arg nanospheres exhibited specific separation capacity towards β-casein or ovalbumin (OVA) in the model protein mixture and remarkably selective enrichment performance for phosphorylated biomolecules in real biological samples (egg white, nonfat milk, and rat brain lysate), showing great potential for trace biological detection and proteomics analysis.
研究领域:
分析化学
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生物化学
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蛋白质组学
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DOI
PubMed
高影响因子
WEED SCIENCE
·
2025
·
IF 2.5
Wei Hu,Yuguo Li,Chaowei Zhao,Liya Zhang,Yumeng Ma,Quan Gao,Haiqun Cao,Min Liao
Barnyardgrass [Echinochloa crus-galli (L.) P. Beauv.] is a dominant weed species occurring in rice fields across China. Metamifop, a common herbicide, is frequently applied to control E. crus-galli and other grassy weeds in rice fields. Herein, HS01, an E. crus-galli population suspected to be resistant (R) to metamifop, was collected from Hanshan County in Anhui Province. Whole-plant dose-response testing revealed that, compared with the susceptible (S) population FD03, HS01 had developed high-level resistance to metamifop with a resistance index (RI) of 11.76 and showed cross-resistance to cyhalofop-butyl (RI=9.33), fenoxaprop-P-ethyl (RI=5.80) and clethodim (RI=3.24). Gene sequencing revealed a Cys-2088-Arg mutation in ACCase 1,5 allele of all the R plants, while ACCase gene overexpression was not involved in the resistance. Molecular docking indicated that the less negative binding energies might be the main reason for the resistance of HS01 to ACCase-inhibiting herbicides. A derived cleaved amplified polymorphic sequence (dCAPS) method was developed for the rapid identification of the Cys-to-Arg mutation in the ACCase gene at codon position 2088 in E. crus-galli. Additionally, pretreatment with the cytochrome P450 inhibitor piperonyl butoxide or the glutathione S-transferase inhibitor 4-chloro-7-nitrobenzoxa-diazole had no significant effects (p> 0.05) on the resistance of HS01 to metamifop. To our knowledge, this is the first report of a Cys-2088-Arg mutation in E. crus-galli ACCase that confers cross-resistance to ACCase-inhibiting herbicides.
研究领域:
植物学
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分子生物学
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农业科学
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2016
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IF 7.145
Shan Yu,Ying Gao,Xu Mei,Tanchen Ren,Su Liang,Zhengwei Mao,Changyou Gao
Selective adhesion and migration of endothelial cells (ECs) over smooth muscle cells (SMCs) is very important in the rapid endothelialization of blood-contacting implants to prevent vascular restenosis. In this study, a uniform cell-resistant layer of methacrylate-functionalized hyaluronic acid (HA) was first immobilized on a poly(ε-caprolactone) (PCL) film via polydopamine coupling. Then, a density gradient of thiol-functionalized Arg-Glu-Asp-Val (REDV) peptide was prepared on the HA layer via thiol-ene click chemistry and the continuous injection method. The REDV gradient selectively enhanced EC adhesion and preferential directional migration toward the region of higher REDV density, reaching 86% directionality in the middle of the gradient. The migration rate of ECs was also significantly enhanced twofold compared with that on tissue culture polystyrene (TCPS). In contrast, the gradient significantly weakened the adhesion of SMCs to 25% of that on TCPS but had no obvious impact on the migration rate and directionality. Successful modulation of the selective adhesion and directional migration of ECs over SMCs on biodegradable polymers serves as an important step toward practical applications for guided tissue regeneration.
研究领域:
生物材料学
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细胞生物学
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DOI
PubMed
高影响因子
BIOMEDICAL CHROMATOGRAPHY
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2022
·Vol. 37
·期 2
·e5535
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IF 1.911
Jiayin Liu,Yang An,Jun Su,Qinghai Dong,Hongliu Xie,Jihua Liu
In this study, a new compound PPD-Arg (Tos) (PAT), an arginine derivative of 20(s)-PPD, was synthesized via Fmoc-Arg (Tos)-OH and 20(s)-PPD. The pharmacokinetic properties in rats, in vitro cytotoxicity, and cell apoptosis rates of protopanaxadiol (PPD) and PAT were determined. A sensitive bioanalytical method for pharmacokinetics using ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry was developed and validated. The result showed that the T max and t 1/2 of PAT were significantly enhanced, indicating a long-lasting effect in vivo . Compared to the PPD group, the PAT group showed higher bioavailability. PAT also exhibited higher antitumor efficacy than PPD against three cancer cells, especially the strongest inhibitory activity against Huh-7, even more potent than the positive control of paclitaxel. Therefore, the apoptosis assay based on annexin V/propidium iodide–combined staining against Huh-7 further demonstrated that PAT could induce apoptosis of Huh-7 cells. Better pharmacokinetic properties and antitumor efficacy of the arginine derivative of 20(s)-PPD were important. These findings could provide references for further clinical research on amino acid derivatives of PPD as antitumor agents.
研究领域:
药理学
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肿瘤学
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分析化学
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DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2024
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IF 4.2
Min Liao,Minghao Jiang,Xumiao Wang,Wei Hu,Ning Zhao,Haiqun Cao
Acetyl-CoA carboxylase (ACCase)-inhibiting herbicides are among the most commonly used herbicides to control grassy weeds, especially Leptochloa chinensis , in rice fields across China. Herein, we collected a suspected resistant (R) population of L. chinensis (HFLJ16) from Lujiang county in Anhui Province. Whole plant dose response tests showed that, compared with the susceptible (S) population, the R population showed high resistance to cyhalofop-butyl (22-fold) and displayed cross-resistance to metamifop (9.7-fold), fenoxaprop- P -ethyl (18.7-fold), quizalofop- P -ethyl (7.6-fold), clodinafop-propargyl (12-fold) and clethodim (8.4-fold). We detected an amino acid substitution (Cys-2088-Arg) in the ACCase of resistant L. chinensis . However, ACCase gene expression levels were not significantly different ( P > 0.05) between R plants and S plants, without or with cyhalofop-butyl treatment. Furthermore, pretreatment with piperonyl butoxide (PBO, a cytochrome P450 monooxygenase (CYP450) inhibitor) or 4-chloro-7-nitrobenzoxadiazole (NBD-Cl, a glutathione-S-transferase (GST) inhibitor), inhibited the resistance of the R population to cyhalofop-butyl significantly (by approximately 60% and 26%, respectively). Liquid chromatography tandem mass spectrometry analysis showed that R plants metabolized cyhalofop-butyl and cyhalofop acid (its metabolite) significantly faster than S plants. Three CYP450 genes, one GST gene, and two ABC transporter genes were induced by cyhalofop-butyl and were overexpressed in the R population. Overall, GST-associated detoxification, CYP450 enhancement, and target-site gene mutation are responsible for the resistance of L. chinensis to cyhalofop-butyl.
研究领域:
植物科学
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分子生物学
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杂草科学
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除草剂抗性
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2022
·
IF 9.231
Songjie Li,Weimin Zhang,Ruimin Wang,Congfa Li,Xue Lin,Lu Wang
Partridge leaves ( Mallotus furetianus Muell-Arg.) have long been consumed as popular folk substitute tea for treating hyperglycemia in China. In this study, the inhibiting effects of partridge tea extracts on α -glucosidase and α -amylase were investigated, and then effect of partridge tea aqueous extracts (PTAEs) on glucose consumption capacity of 3 T3-L1 preadipocytes cells was determined. Results verified that PTAEs showed excellent anti- α -glucosidase and anti- α -amylase effects. In addition, the PTAEs evidently promoted glucose consumption capacity of 3T3L1 preadipocytes cells. To this end, a combined method of affinity ultrafiltration and HPLC-ESI-qTOF-MS/MS was used for rapidly screening and identifying the potential inhibitors in the PTAEs. Catechin, epicatechin, rutin, ferulic acid, and kaempferitrin with high affinity capacity indicated strong inhibiting effect on α -glucosidase and α -amylase. Docking studies revealed the potential interactive mechanisms between these major inhibitors and two digestive enzymes. This research shows that partridge tea is effective in preventing and treating post hyperglycemia.
研究领域:
药理学
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生物化学
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营养学
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分子生物学
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植物科学
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产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2023
·
IF 4.4
Xiaoxuan Cai,Miaoen Huang,Xixiang Huang,Huan Liu,Tianji Wang,Li Li,Weiguang Yang,Hui Luo,Yingnian Lu
The organism Sipunculus nudus L is a good resource for marine peptides because of its rich protein. However, the disadvantage of biological peptides is their short metabolic half-life, therefore, structural modification of peptides is necessary. In this research, the enzymatic hydrolysis of Sipunculus nudus L was performed using trypsin and then peptides were isolated and purified from the hydrolysates, four novel tripeptides were identified by LC-MS-MS as Ser-Arg-Pro (SRP, m/z = 358.39), Pro-Arg-Pro (PRP, m/z = 368.43), Arg-Pro-Ala (RPA, m/z = 342.39), and Lue-Pro-Lys (LPK, m/z = 356.46), their ACE inhibitory activity was investigated with IC 50 value as following 0.046, 0.42, 0.97, and 6.54 mM, respectively. Because of its highest ACE inhibitory activity, SRP was further selected to modify the chemical structure for a sustained release agent DSPE-PEG-SRP. Compared with 7 h of free SRP, the release of DSPE-PEG-SRP in vitro was approximately 120 h. The ACE inhibition rate of SRP released from the synthetic product and the raw material SRP was almost the same, molecular docking demonstrated that ACE inhibitory activity was attributed to the formation of hydrogen bonds between SRP and ACE active sites . In conclusion, DSPE-PEG-SRP, a novel sustained release agent, has great potential and is expected to be further applied to treat hypertension.
研究领域:
海洋生物学
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药学
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生物化学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Baichuan Mu,Chuanhang Zhou,Yang Liu,Zhe Fan,Yiran Li,Ruiqi Wang,Wenlong Yan,Xicheng Duan,Wei Guo,Zongsheng Yin,Yinzhong Chen
The physical interfaces governing macromolecular protein–protein interactions dictate critical gene transcription programs, yet the structural basis of regulatory complexes inhibiting NF-κB remains partially resolved. Here, we characterize the macromolecular interaction between Smad nuclear interacting protein 1 (SNIP1) and the transcriptionally active p65 subunit of NF-κB, a regulatory axis whose pathological downregulation was identified via comparative proteomics of human osteoarthritic cartilage. GSE114007 and descriptive single-cell analyses of GSE152805 showed lower SNIP1 expression in diseased cartilage; GSE169077 showed the same direction without statistical significance. Co-immunoprecipitation confirmed the endogenous physical association of SNIP1 and p65 in human chondrocytes. Molecular docking and molecular dynamics (MD) simulations mapped a candidate SNIP1–p65 interface with a negative MM/GBSA endpoint estimate (ΔGbind = −209.6 kJ/mol, 95% CI −381.4 to −37.8 kJ/mol across three trajectory means). The computational models position SNIP1 Arg-287 and Arg-288 within a cationic interface that is sterically incompatible with the DNA-bound conformation of p65. Computational substitution analysis further identified Arg-288 as a candidate hotspot residue, with charge-reversal substitutions predicted to destabilize the complex; this residue-level prediction awaits experimental site-directed mutagenesis. An assumption-based ODE model illustrates the sensitivity of downstream outputs to imposed SNIP1-dependent inhibition without establishing clinical kinetics or residue-specific causality. Overall, the combined data define a testable structural model for SNIP1-mediated regulation of p65 while avoiding causal attribution to Arg-288 before experimental validation.
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DOI
高影响因子
SCIENTIA HORTICULTURAE
·
2026
·
IF 4.6
Rui-Ping Feng,Han-Li Liu,Mao-Song Pei,Rui-Min Hu,Charles Ampomah-Dwamena,Xin-Jie Yang,Yun-Lu Song,Ding-Ding Zuo,Guo-Hao Wang,Tong-Lu Wei,Da-Long Guo,Hai-Nan Liu
CHI-Phe-Arg coating maintains the postharvest quality of ‘Kyoho’ grape berries. • Transcriptomics reveals VviNAC25/83 as key regulators of postharvest quality. • CHI-Phe-Arg regulates VviCESA5 via the VviNAC25/83 complex to delay fruit softening. Table grapes ( Vitis vinifera L.) are highly susceptible to postharvest softening and decay. This study investigated the preservative effects and molecular mechanisms of a composite coating consisting of chitosan (CHI) combined with phenylalanine (Phe) and arginine (Arg), designated as CHI-Phe-Arg, on ‘Kyoho’ table grapes. This composite coating significantly suppressed postharvest decay, berry abscission, and weight loss, inhibited the activities of cell wall-degrading enzymes (PG and Cx), and delayed fruit softening. To elucidate its molecular mechanism, transcriptome sequencing was performed on grape berries at 3 d, 6 d, and 9 d post-treatment. Transcriptome analysis identified two significantly upregulated NAC transcription factors, VviNAC25 and VviNAC83, as well as the cellulose synthase gene VviCESA5 . Promoter analysis revealed the presence of NAC-binding sites (CATGTG/CGTA/TACG/CGTG) in the VviCESA5 promoter. Dual-luciferase and yeast one-hybrid assays confirmed that VviNAC25 and VviNAC83 directly bind to and activate the transcription of VviCESA5 , exhibiting a synergistic effect. Luciferase complementation imaging (LCI) and bimolecular fluorescence complementation (BiFC) assays further confirmed the interaction between VviNAC25 and VviNAC83, supporting their formation of a transcription factor complex. Transient overexpression assays further demonstrated that co-expression of VviNAC25 and VviNAC83 significantly enhanced the expression of VviCESA5 . In conclusion, the CHI-Phe-Arg composite coating delays grape softening by activating the VviNAC25/VviNAC83- VviCESA5 transcriptional module, providing a theoretical basis for developing chitosan-amino acid composite coating technologies for grape preservation. Download: Download high-res image (191KB) Download: Download full-size image
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Yan-Yan Chen,Qi Lin,You-Ming Zhang,Hong Yao,Tai-Bao Wei,Yan-Qing Fan,Xiao-Wen Guan,Guan-Fei Gong,Qi Zhou
Recently, ultrasensitive detection and multi-guest sensing have received extensive attention due to their high sensitivity and efficiency. Herein, we report a novel approach to achieve ultrasensitive detection of multi-analyte. This approach is concluded as “rationally introduce Aggregation-Induced Emission (AIE) into chemosensor”. According to this approach, by rationally introducing self-assembly moiety, the obtained chemosensor DNS could serve as a novel AIEgen and show strong AIE in DMSO/H 2 O (water fraction 80%) binary solution. Interestingly, a simple fluorescent sensor array based on the DNS has been developed. This sensor array could selectively sense Fe 3+ , Al 3+ , H 2 PO 4 − and L-Arg in water solution. More importantly, this sensor array shows ultrasensitive detection for Fe 3+ , Al 3+ and L-Arg. The LODs of the sensor array for Fe 3+ , Al 3+ and L-Arg are in the range of 3.54 × 10 −9 M to 9.42 × 10 −9 M. Moreover, H 2 PO 4 − could realize the reversible detection of Fe 3+ in the DMSO/H 2 O (water fraction 80%) solution. Meanwhile, DNS -based test papers and thin films were prepared, which could serve as test kits for convenient detection Fe 3+ , Al 3+ , and L-Arg in water. In addition, they could also act as efficient erasable fluorescent display materials.
研究领域:
分析化学
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材料科学
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DOI
PubMed
高影响因子
ACS Applied Polymer Materials
·
2026
·
IF 5.2
Limo Wang,Bingjie Xu,Xinting Dong,Danfeng Jian,Tianming Lu,Yujie Gao,Jindan Wu
The misuse of antibiotics has resulted in multidrug resistance among bacteria, rendering nearly all conventional antibiotics ineffective and creating a pressing global need for highly efficient and broad-spectrum antibacterial alternatives. Photodynamic antibacterial therapy (PDAT) has emerged as a promising strategy to address bacterial resistance; however, its efficacy is limited by inherent challenges such as poor tissue penetration, short diffusion ranges, and brief half-lives of reactive oxygen species (ROS). In this study, we developed an antibacterial composite nanofiber membrane with red light responsiveness by incorporating functional l-arginine-modified PCN-224 nanoparticles (Arg-PCN NPs), which enabled NO-enhanced synergistic PDAT. Specifically, Arg-PCN NPs were synthesized and subsequently embedded into a polylactic acid (PLA) matrix by electrospinning, yielding a porous nanocomposite membrane (APM). Under irradiation, APM showed a strong synergistic antibacterial effect, and the bactericidal rate against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) reached 99.9%. Comprehensive mechanistic studies revealed that APM potently enhanced bacterial membrane permeability and elevated intracellular ROS levels upon irradiation. Furthermore, cytotoxicity assays confirmed that APM exhibited negligible in vitro cytotoxicity. The red light-activated antibacterial membrane developed in this work holds significant potential for wound care applications and provides a design strategy for advanced antibacterial materials capable of combating drug-resistant bacterial infections.
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DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
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IF 4.6
Yunjing Zang,Huifeng Zhang,Dawei Zhang,Qiong Jia
Fenitrothion (FTN), a widely used organophosphorus pesticide, raises significant concerns for food safety and environmental health, creating a demand for sensitive and rapid detection methods. In this study, as the first attempt, a red-emitting gold nanocluster (Arg/MTU-AuNCs) was synthesized for FTN detection. The introduction of arginine (Arg) and 4-hydroxy-2-mercapto-6-methylpyrimidine (MTU) significantly improves the fluorescence properties of AuNCs via Au-S and hydrogen bonds. After the addition of FTN, the fluorescence intensity of Arg/MTU-AuNCs varies with FTN concentration due to the static quenching, thus enabling its sensitive detection. The probe exhibits a linear detection range for FTN of 2.00-90.00 μg/L and a limit of detection of 0.72 μg/L. Furthermore, the probe exhibits high selectivity against common interfering substances (e.g., metal ions, anions, and coexisting pesticides). When used for analysis of real samples including tea, mulberry leaves, and oranges, the method achieves recoveries ranged from 90.50% to 108.67%. In addition, a fluorescent hydrogel sensing platform was constructed by combining the probe with agarose hydrogel, and portable detection of FTN was realized through a smartphone. This work offers an effective and portable approach for rapid FTN monitoring, with significant potential for food safety and environmental applications.
研究领域:
分析化学
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环境科学
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食品安全
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纳米材料
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传感技术
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）