JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2022
·
IF 4.52
Chujun Liu,Xing Xie,Yawen Guo,Bo Wang,Kaizhou Xie,Yuhao Dong,Chenggen Yang,Zhixin Feng,Wenbin Bao
A novel, simple and high-sensitivity gas chromatography-tandem mass spectrometry (GC-MS/MS) method combined with external calibration was developed for the qualitative and quantitative determination of penicillin G residues in poultry tissues and pork. Samples were extracted by an ASE 350 apparatus with 0.2 mol/L phosphate buffer solution (pH 8.0) and purified on HLB solid-phase extraction (SPE) cartridges (60 mg/3 mL). Trimethylsilyl diazomethane (TMSD) was selected as the derivatization reagent for precolumn derivatization. The recoveries of analytes at the limit of quantitation (LOQ) and 25, 50 and 100 μg/kg ranged from 80.67% to 96.18%, with RSDs below 3.17%. The determination coefficient (R 2 ) values were above 0.9993 in the corresponding concentration range. The limits of detection (LODs) (S/N ≥ 3) and LOQs (S/N ≥ 10) ranged from 1.50 to 4.10 μg/kg and 4.50–8.20 μg/kg, respectively. In addition, the newly established method was implemented to analyze poultry tissues and pork purchased from a local market, demonstrating the sensitivity and applicability of the method.
研究领域:
分析化学
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食品安全
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兽医药理学
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DOI
高影响因子
Foods
·
2021
·Vol. 10
·期 11
·2735
·
IF 4.35
Chujun Liu,Yawen Guo,Bo Wang,Lan Chen,Kaizhou Xie,Chenggen Yang
A simple and sensitive gas chromatography–tandem mass spectrometry (GC–MS/MS) method was established for the quantitative screening of penicillin G residues in chicken and duck eggs (whole egg, yolk and albumen). The analyte was separated on a TG-1MS capillary column (30.0 m × 0.25 mm i.d., 0.25 μm) with an external calibration method and electron impact (EI) ionization. Samples were pretreated using an accelerated solvent extraction (ASE) procedure followed by solid-phase extraction (SPE) on HLB cartridges (60 mg/3 mL). The derivative, which was safer and easier to store than penicillin G, was obtained by reacting trimethylsilyl diazomethane (TMSD) with penicillin G. The method was validated by the following parameters: linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ). The matrix-matched calibration curves had good linearity (R2 ≥ 0.9994) within the concentration range of LOQ–200.0 µg/kg for penicillin G in the sample matrices. In the same concentration range, the accuracy, in terms of recovery, was 80.31–94.50%; the relative standard deviation (RSD), intra-day RSD and inter-day RSD ranged from 1.24 to 3.44%, 2.13 to 4.82% and 2.74 to 6.13%, respectively. The LODs and LOQs of penicillin G in the matrices were in the ranges of 1.70–3.20 and 6.10–8.50 μg/kg, respectively. The applicability of the GC–MS/MS method was demonstrated by the determination of poultry eggs obtained from local markets with no penicillin G residues.Keywords:penicillin G;GC–MS/MS;ASE;poultry eggs;SPE
研究领域:
分析化学
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食品安全
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产品关联
DOI
PubMed
高影响因子
Photonics Research
·
2021
·Vol. 9
·期 10
·1916-1923
·
IF 7.08
Qingyun He,Enrou Mei,Ze Wang,Xiaojuan Liang,Xiaojuan Liang,Suqin Chen,Suqin Chen,Weidong Xiang
Here, Gd-doped CsPbCl1.5Br1.5 nanocrystals (NCs) in borosilicate glass matrix (B2O3−SiO2−ZnO) were prepared by melting quenching and in-situ crystallization. The optical performance of CsPbCl1.5Br1.5 NCs glasses under different heat-treatment temperatures and the content of Gd3+ were analyzed in detail. After CsPbCl1.5Br1.5 NCs glass is doped with Gd3+ ions, the photoluminescence intensity increases and the synthesized Gd-doped CsPbCl1.5Br1.5 NCs glasses have excellent water stability and thermal cycling performance. In addition, the influence of Gd-doped concentrations and heat-treatment temperatures on the amplified spontaneous emission (ASE) thresholds of CsPbCl1.5Br1.5 NCs glasses was studied, and the Gd-doped CsPbCl1.5Br1.5 NCs glasses achieve controllable ASE thresholds at room temperature. The ASE threshold can be as low as 0.39 mJ/cm2. This work offers a neoteric reference for the research in the application of metal ion-doped perovskite NCs and a new idea for the realization of controllable and low ASE thresholds on perovskite NCs.
研究领域:
材料科学
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光学工程
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产品关联
DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2025
·
IF 6.3
Yufan Xu,Bo Huang,Bin Liu,Shitong Li,Huichao Zhang,Hongyu Yang,Ruilin Xu
Due to their remarkable suitability for mass production, micro-scale flow strategies for inorganic perovskite nanocrystals (NCs) are rapidly developed. However, the existing microfluidic systems belong to single-stage thermal reactors and cannot achieve temperature adjustment during NC preparation like the hot-injection method to attain beneficial crystallization and growth temperatures. In this work, we designed two-stage microfluidic system with two heating reaction modules to carry out the growth of CsPbBr 3 NCs experiencing dual reaction temperatures in flow. The nanoplatelets formed due to lower precursor combination temperatures in the first stage are mostly transformed into cuboidal NCs by a second heating reaction, improving the stability of the CsPbBr 3 NCs. Furthermore, the mixed morphology probably impedes the amplified spontaneous emission (ASE) performance, so uniform cuboidal NCs (average size of 18.3 nm) have a lower ASE threshold of 4.6 μJ cm −2 at room temperature. Additionally, this system effectively avoids the precipitation of PbBr 2 precursor at elevated temperatures, thus enabling the successful fabrication of larger-sized NCs (28.2 nm) upon raising the preparation temperature to 250 °C.
研究领域:
材料科学
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纳米技术
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光学
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产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2020
·Vol. 100
·期 12
·4565-4574
·
IF 2.614
Zhaoxian Huang,Zhenyu Cao,Zengwang Guo,Liang Chen,Zhongjiang Wang,Xiaonan Sui,Lianzhou Jiang
BACKGROUND Medium- and long- chain triacylglycerols (MLCTs) are functional structural lipids that can provide the human body with essential fatty acids and a faster energy supply. This study aimed to prepare MLCTs rich in α -linolenic by enzymatic interesterification of perilla oil and medium-chain triacylglycerols (MCTs), catalyzed by Lipozyme RM IM, Lipozyme TL IM, Lipozyme 435, and Novozyme 435 respectively. RESULTS The effects of lipase loading, concentration of MCTs, reaction temperature, and reaction time on the yield of MLCTs were investigated. It was found that the reaction achieved more than a 70% yield of MLCTs in triacylglycerols under the conditions of 400 g kg −1 MCTs and 60 g kg −1 lipase loading after equilibrium. A novel two-stage deodorization was also applied to purify the interesterification products. The triacylglycerols reach over 97% purity in the products with significant removal ( P < 0.05) of the free fatty acids, and the trans fatty acids were strictly controlled at below 1%. There was more than 40% α -linolenic in the purified products, with long-chain fatty acids mostly occupying the desired sn -2 position in acylglycerols, which are more active in hydrolysis. CONCLUSION A series of novel α -linolenic acid-rich medium- and long-chain triacylglycerols was prepared. Under appropriate reaction conditions, the yield of MLCTs in triacylglycerols was above 70%. A novel two-stage deodorization can be used to promote the elimination of free fatty acids and limit the generation of trans fatty acids. © 2020 Society of Chemical Industry
研究领域:
食品科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
PHARMACEUTICAL BIOLOGY
·
2019
·
IF 2.492
Chunfang Zhang,Wenfeng Zhang,Rongyu Shi,Bangyi Tang,Shicheng Xie
Context: Adlay seed [Job’s tears, Coix lachryma-jobi L. var. ma-yuen Stapf (Poaceae)] is a Traditional Chinese Medicine, which has been investigated to treat inflammatory diseases and rheumatism.Objective: This study evaluates the ameliorative effects of adlay seed extract (ASE) in a complete Freund’s adjuvant (CFA)-induced rheumatoid arthritis (RA) rats.Materials and methods: The RA Sprague–Dawley rat model was induced and randomly divided into six groups with or without ASE treatment (50, 100 or 200 mg/kg). After 28 d administration, the symptoms, biochemical parameters and molecular mechanisms were investigated.Results: The values of paw oedema, PGE2 and MMP-3 decreased from 1.46 ± 0.04 to 0.66 ± 0.07 cm3, from 126.2 ± 11.48 to 79.71 ± 6.8 pg/mL and from 142.7 ± 8.36 to 86.51 ± 5.95 ng/mL, respectively; the values of body weight increased from 177.25 ± 5.94 to 205 ± 6.52 g in HASE group. In addition, treatment of ASE reduced the levels of pro-inflammatory cytokines (IL-1β, TNF-α, IL-6, MCP-1), and increased the activities of antioxidant enzyme (GSH-Px, SOD, and CAT). Furthermore, ASE could suppress the mRNA expression of COX-2 and CHI3L1 and improve the mRNA expression of CAT and GPx-1 in ankle tissues of RA rats.Discussion and conclusions: For the first time, our results indicated ASE exerts anti-RA effects via inhibiting pro-inflammatory factors and alleviating oxidative stress. Our finding sheds light on the research and development of anti-RA functional foods from adlay seed.
研究领域:
药理学
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免疫学
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中医药
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2025
·
IF 4.6
Chunlan He,Yunxi Tang,Shiqi Chen,Siyu Huang,Youming Xiong,Yamin Bai,Jingbing Xu,Yi Zhong,Hong Cheng,Lei Wang,Zhong Chen,Changjun Hou
Per- and polyfluoroalkyl substances (PFASs) have been widely detected in animal samples. But the fats, indispensable tissue in animals, can seriously hinder the PFASs extraction due to their nature of complex, unbreakable, and concretionary. To address this, a new sample preparation method, combining the advantages of ASE and QuEChERS, is proposed. Firstly, the extraction conditions of new method are optimized as co-solvent of MeCN+H 2 O, temperature of 100 °C, and time of 30 min. The MeCN performs better than MeOH because the MeOH will bring some impurities (phospholipids). The H 2 O under high temperature plays important dual functions of digestion and organic solvent. The exothermic reaction of MgSO 4 also enhance the PFASs extraction. Next, 15 animal samples are screened by both the new ASE+QuEChERS and traditional QuEChERS. The lower and upper limits of quantitation are 0.25 ng g −1 and 25.00 ng g −1 , respectively. Nine PFASs are detected in five positive simples. The new method can improve the total concentration of PFASs by range from 6 % to 64 %. Lastly, both the new ASE+QuEChERS and traditional QuEChERS methods are validated based on 10 relevant food simples. This study provides a simple, efficient and practical method for extraction of PFASs from animal samples.
研究领域:
分析化学
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环境科学
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食品安全
·
毒理学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
·
IF 5.7
Yuhan Qiu,Chuanshuang Hu,Jiewen Li,Qin Lai,Ziling Liu,Xiuyi Lin,Weiwei Zhang
Although γ-methacryloxypropyltrimethoxysilane (MPS) was proved to be an effective reagent for improving the dimensional stability of wood, a bottleneck in ASE value (around 50%) existed. The reason was that MPS with low polarity opened few hydrogen bonds in the amorphous region of cellulose, while these hydrogen bonds could be reopened by water. Therefore, citric acid (CA) is chosen to cooperate with MPS to further enhance the dimensional stability of wood. In this paper, MPS and CA were used to modify wood individually (MW and CW) or with different combinations, that is, one-step modification (M/CW) and two-step modification with MPS first (M-CW) or CA first (C-MW). CA and MPS concentrations were optimized at 5 wt%. The ASE value for M/CW was only 25.74% at a weight percent gain (WPG) of 6.43%, which was only 0.6 times to MW or 0.7 times to CW. For M-CW, the ASE value gradually decreased with the soaking cycles, from 65.64% at a WPG of 9.05% to 51.20%. The C-MW had the best dimensional stability, with the ASE value 75.35% at a WPG of 11.50%. Although it decreased during the first soaking cycle, it stabilized at 62.20% at last. SEM and EDS images showed that the polymer mainly distributed in cell walls and few in cell lumen in C-MW. Thus, the enhanced dimensional stability of C-MW could be explained by CA opening the hydrogen bonds in the amorphous region of cellulose first, which provided more binding sites for MPS.
研究领域:
材料科学
·
木材科学与技术
·
化学工程
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产品关联
DOI
PubMed
高影响因子
Cell Reports
·
2024
·
IF 7.5
Huimin Liu,Panpan Chen,Xubo Yang,FanRui Hao,Guojing Tian,Zhao Shan,Bin Qi
Animals constantly face microbial challenges, and microbe-mediated infection protection is crucial for host survival. Identifying specific bacteria and their interactions with host intracellular surveillance systems is important but challenging. Here, we develop a “probiotics” screening system that identifies Escherichia coli mutants, such as ΔymcB , which protect hosts from Pseudomonas aeruginosa PA14 infection by activating the mitochondrial unfolded protein response (UPR mt ). Genetic screening reveals that MDSS-1, a neuronal transmembrane protein, is crucial for sensing ΔymcB and triggering intestinal UPR mt . MDSS-1 functions as a potential receptor in ASE neurons, detecting ΔymcB and transmitting signals through neuropeptides, GPCRs, Wnt signaling, and endopeptidase inhibitors to activate intestinal UPRmt and enhance protection. Constitutive activation of MDSS-1 in ASE neurons is sufficient to induce UPR mt and confer infection resistance. This study uncovers a neuron-intestine communication mechanism, where ASE neurons detect bacteria and modulate the intestinal mitochondrial surveillance system for host adaptation to pathogens.
研究领域:
微生物学
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神经科学
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免疫学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
ADVANCED MATERIALS
·
2025
·
IF 26.8
Changjiang Shi,Haihua Zhang,Haolun Li,Hongbing Fu
Blue-green lasers are essential for next-generation optoelectronics. While GaN-InGaN lasers offer excellent performance, their complex fabrication and challenges in precise bandgap tuning remain significant limitations. Hybrid lead halide perovskites present a promising alternative, with tunable bandgaps and cost-effective solution processing. However, halide migration in these materials causes spectral instability and luminescence shifts under operational conditions. Here, a novel class of Rb-Cs alloyed quasi-2D perovskites is introduced that achieve both low amplified spontaneous emission (ASE) thresholds and exceptional spectral stability. By precisely tuning the Rb-Cs ratio, it realizes ASE across the blue-green spectrum (481–532 nm). Rb-Cs alloying critically modulates the quantum well distribution, optimizes the energy landscape, and suppresses Auger recombination, resulting in a record-low ASE threshold of 1.94 µJ·cm −2 —over 50% lower than that of mixed-halide perovskites. These materials exhibit remarkable spectral robustness, with ASE spectra remaining stable even after prolonged thermal annealing, thereby overcoming halide migration-induced degradation. Furthermore, micro-ring laser arrays fabricated from these Rb-Cs alloyed perovskites demonstrate superior lasing characteristics, including high-quality whispering-gallery-mode resonances and low lasing thresholds, underscoring their potential for advanced laser technologies.
研究领域:
光电子学
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材料科学
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光子学
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半导体激光器
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钙钛矿光电器件
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激光技术
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Zhenju Bi,Xinqi Gao,Zhencui Bi,Li Yan,Yafang Lei,Chan Li
The wood used for construction should be able to cope with complex environments, especially water and decay fungi. To enhance the wood’s decay resistance and hydrophobicity, aromatic aldehydes such as vanillin, cinnamaldehyde, syringaldehyde, or protocatechualdehyde were grafted onto wood. The poplar ( Populus tomentosa Carr.) wood was impregnated with (3-aminopropyl) triethoxysilane (APTES) to aminate its cell wall, and then aromatic aldehydes were grafted onto the aminated wood through a Schiff base reaction. The findings indicated that this technique enhanced the leaching resistance of aromatic aldehydes in the wood. Furthermore, the durability level of wood grafted by vanillin or cinnamaldehyde was very durable (mass loss<5 %) even after the leaching test. Modified wood has hydrophobicity, manifested by strong anti-swelling efficiency (ASE), low equilibrium moisture content (EMC), and high water contact angle (>90°, air-dried) in 65 % relative humidity and saturated environments. The characterization of modified wood and its model compounds indicated that aromatic aldehydes were grafted onto the hydroxyl groups of wood through Schiff base under the mediation of APTES. This study obtained an outdoor green building wood with hydrophobic and decay resistance for outdoor use and expanded the application of aromatic aldehydes in wood.
研究领域:
材料科学
·
木材科学与技术
·
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）