Journal of Water Process Engineering
·
2023
·
IF 7
Hao Sun,Xiaoqing Li,Xiaobing Li
The asphaltene-rich alkaline-surfactant-polymer (ASP) flooding produced water has been identified with high interfacial stability and severe secondary emulsification, which poses great difficulties in its demulsification process. This leads to an urgent need to develop targeted and efficient demulsifiers. In this research, a novel polydopamine-polyquaternium modified Fe 3 O 4 (PQA-PDA@Fe 3 O 4 ) nanoparticle integrates the electrical neutralization, interfacial adsorption and magnetic aggregation functionalities was prepared to dispose this emulsion. Primarily, structural and surface properties of the PQA-PDA@Fe 3 O 4 demulsifier were systemically characterized by a series of analytical techniques, which presents its core-shell, hydrophilic and positively charged characters. When employing PQA-PDA@Fe 3 O 4 to treat the produced water that contained 500 mg/L emulsified oil, a demulsification efficiency of 90.5 % was achieved at 200 mg/L dosage. It was found that the PQA-PDA@Fe 3 O 4 nanoparticles could coalesce the fine oil droplets into large and dense aggregates, as demonstrated by the raised fractal dimension values. By monitoring the interfacial properties (interfacial tension, zeta potential and interfacial film elastic modulus) variations, the demulsification mechanism was further explored. The PQA-PDA@Fe 3 O 4 nanoparticle could spontaneously migrate to the oil/water interface and reassemble a loose interfacial film, thus leading the oil droplets destabilization and aggregation. Notably, the magnetic force between the PQA-PDA@Fe 3 O 4 nanoparticles significantly accelerates the oil droplets aggregation and inhibits the secondary emulsification process, so as to achieve the outstanding demulsification efficiency. Moreover, this PQA-PDA@Fe 3 O 4 nanoparticle exhibited acceptable magnetic recyclability. These results provided an effective and alternative protocol to demulsify the asphaltene-rich ASP flooding emulsions.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
LANGMUIR
·
2022
·
IF 4.331
Xiaoqing Li,Dandan Liu,Hao Sun,Xiaobing Li
In this work, the effect of oil-displacing agent composition on oil/water interface stability of the asphaltene-rich alkali–surfactant–polymer (ASP) flooding-produced water was systematically investigated, especially from the perspective of the interaction between oil displacement agents and asphaltene at the oil/water interface. Primarily, adsorption behavior of the artificial and natural interfacial substances (oil displacement surfactant and asphaltenes) on the oil/water interface was investigated by molecular dynamics simulation. The oil displacement surfactant and asphaltenes formed a cross-linked and compact interfacial film structure, which significantly enhanced the interface stability; the more the oil displacement surfactants adsorbed on the interface, the more stable is the cross-linked structure formed between them and asphaltenes. Then, the interfacial property variations that are originating from the interactions differences between oil displacement agents and asphaltenes were monitored via interfacial tension, zeta potential, and interfacial film rheology tests. Moreover, the effect of oil displacement agent concentrations on the interfacial film thinning and rupture kinetic behavior was further investigated. Finally, cream experiments were conducted to verify the effect of oil displacement agent composition on the oil/water separation efficiencies of asphaltene-rich ASP flooding-produced water. When 5% asphaltenes was added, the creaming oil removal rate reduced from 90.0 to 85.3% at 19 h. The interactions between asphaltenes and oil displacement agents immensely enhance the oil/water interfacial film strength and impede the oil/water separation process.
研究领域:
石油工程
·
界面化学
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DOI
PubMed
高影响因子
JOURNAL OF DRUG TARGETING
·
2016
·
IF 2.821
Lijing Zhang,Hua Cao,Jiaxin Zhang,Chengli Yang,Tingting Hu,Huili Li,Wu Yang,Gu He,Xiangrong Song,Aiping Tong,Gang Guo,Rui Li,Yu Jiang,Jiyan Liu,Lulu Cai,Yu Zheng
Specific delivery of drugs to bone tissue is very challenging due to the architecture and structure of bone tissue. A seven-repeat sequence of aspartate, a representative bone-targeting oligopeptide, is preferentially used for targeted therapy for bone diseases. In this study, Asp7-cholesterol((Asp)7-CHOL) was synthesized and (Asp)7-CHOL-modified liposome loaded with doxorubicin (DOX) was successfully prepared using both pre-insertion (pre-L) and post-insertion (post-L) methods. The formulation was optimized according to particle size, zeta potential and the drug-loading efficiency of the liposome. In addition, the bone affinity of the (Asp)7-CHOL-modified liposome was evaluated using a hydroxyapatite (HA) absorption method. The results suggested that (Asp)7-CHOL-modified liposome show excellent HA absorption; pre-L showed slightly higher HA binding than post-L. However, post-L had a higher DOX entrapment efficiency than pre-L. In vivo imaging further demonstrated that pre-L showed a higher bone-targeting efficiency than post-L, which was consistent with in vitro results. In all, (Asp)7-CHOL-modified liposome showed excellent bone-targeting activity, suggesting their potential for use as a drug delivery system for bone disease-targeted therapies.
研究领域:
药学
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生物医学工程
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Shan Yu,Ying Gao,Xu Mei,Tanchen Ren,Su Liang,Zhengwei Mao,Changyou Gao
Selective adhesion and migration of endothelial cells (ECs) over smooth muscle cells (SMCs) is very important in the rapid endothelialization of blood-contacting implants to prevent vascular restenosis. In this study, a uniform cell-resistant layer of methacrylate-functionalized hyaluronic acid (HA) was first immobilized on a poly(ε-caprolactone) (PCL) film via polydopamine coupling. Then, a density gradient of thiol-functionalized Arg-Glu-Asp-Val (REDV) peptide was prepared on the HA layer via thiol-ene click chemistry and the continuous injection method. The REDV gradient selectively enhanced EC adhesion and preferential directional migration toward the region of higher REDV density, reaching 86% directionality in the middle of the gradient. The migration rate of ECs was also significantly enhanced twofold compared with that on tissue culture polystyrene (TCPS). In contrast, the gradient significantly weakened the adhesion of SMCs to 25% of that on TCPS but had no obvious impact on the migration rate and directionality. Successful modulation of the selective adhesion and directional migration of ECs over SMCs on biodegradable polymers serves as an important step toward practical applications for guided tissue regeneration.
研究领域:
生物材料学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Kai Si,Tingting Gong,Suyun Ding,Huiping Liu,Shuyuan Shi,Jianqiu Tu,Linxian Zhu,Li Song,Lushan Song,Xiaowei Zhang
Phosvitin has excellent calcium binding capacity, related to its phosphopeptides. The phosphopeptides may be used as functional ingredients for improving calcium bioavailability, but the calcium-binding mechanism is unclear. In this study, a novel phosvitin phosphorylated pentapeptide (Glu-Asp-Asp-pSer-pSer, EDDpSpS) was selected to prepare an EDDpSpS calcium complex (EDDpSpS-Ca), and the calcium-binding mechanism and bioavailability investigated. The calcium-binding capacity of EDDpSpS was up to 468 ± 152.80 mg/g. Calcium ions prompted the folding of the EDDpSpS structure to form spherical nanoparticles. The calcium binding sites of EDDpSpS involved peptide bonds, carboxyl, amino, and phosphate groups. Molecular forces involved in these interactions were electrostatic in nature. Moreover, EDDpSpS-Ca had excellent bioavailability when compared to CaCO 3 , calcium lactate, and d -calcium gluconate. This study revealed the calcium-binding mechanism of phosvitin phosphopeptide, and suggested that EDDpSpS-Ca has the potential to be a novel, efficient, and promising calcium supplement.
研究领域:
营养学
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生物化学
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DOI
PubMed
高影响因子
Heliyon
·
2024
·
IF 3.4
C H E N Wen-Tao,Ying-Yang Zhang,Qiang Qiang,Ping Zou,Ying Xu,Chengjun Sun,Iftikhar Hussain Badar
In this study, we investigated the antioxidant properties of dry-cured beef crude peptide (BPH) at different storage periods. The combination characteristics of different concentrations of Phe-Asp-Gly-Asp-Phe (FDGDF) and superoxide dismutase (SOD) at different temperatures were analyzed by ultraviolet-visible spectroscopy, fluorescence spectroscopy, and FT-IR spectroscopy, combined with the detection of a SOD activity detection box. It was found that FDGDF could improve the activity of SOD by changing its secondary structure. Bonds were formed at O32/O40/O52 using quantum chemical simulation calculations, and the Fukui index was higher than that of most atoms, indicating that these atoms were more likely to participate in the reaction. SPR biological force analysis showed that FDGDF and SOD were in a fast binding and dissociation mode. This study revealed the theoretical basis for studying the antioxidant mechanism of dry-cured beef and provided ideas for developing new dry-cured beef products.
研究领域:
食品科学
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生物化学
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分子生物学
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DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACOLOGY
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2016
·
IF 2.73
Ming Zong,Dan-Dan Fan,Shan Lin,Yan-Ping Song,Ze-Yu Wang,Xiao-Liang Ma,Wei-Hua Qiu,Yu-Hua Bai,Lei Li,Sen Li
Aspirin has been used in the treatment and chemoprevention of many malignant cancers. The mechanism of its anti-cancer activity mainly involves the inhibition of cyclooxygenase-2 (COX-2). However, the application of aspirin is limited by the serious gastric mucosal damage that accompanies its usage. We have previously reported the preparation of a novel aspirin derivative that we named Ca-Asp, and showed that it causes less damage to gastric mucosa of rat and inhibits the expression of COX-2 to higher degree than Asp. However, the anti-cancer effect and mechanism of Ca-Asp was not demonstrated. In this study, the anti-cancer effect of Ca-Asp was investigated and compared with those of Asp and Hydroxyapatite (Hap) at the cell level. The results showed that treatment of SGC-7901 cells (human gastric cancer cell line) with 200–400 μg/ml Ca-Asp resulted in significant reduction in cell viability , compared to treatment with either Asp or Hap, and at a higher concentration (500 μg/ml). Subsequent investigation into the possible underlying mechanism showed that Ca-Asp induced apoptosis and caused cell cycle arrest at the G1 phase . Ca-Asp also up-regulated the levels of caspase-3 and p53, but down regulated the level of cyclin D1 , NF-κB, COX-2 and PGE 2 . Furthermore, simultaneous treatment of SGC-7901 cells with Ca-Asp and exogenous PGE 2 reduced the anti-proliferative effect of Ca-Asp on the cells. Taken together, the results suggested that Ca-Asp might act as a potential anti-cancer drug, and that its suppression of PGE 2 production might constitute an important part of its anti-cancer activity.
研究领域:
肿瘤学
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药理学
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分子生物学
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DOI
PubMed
高影响因子
Journal of Functional Foods
·
2025
·
IF 4
Tianrui Xue,Tingting Gong,Wei Zhao,Mingran Zhang,Binfei Lv,Huiping Liu,Qi Xu,Xiaowei Zhang
Food-derived peptides with strong calcium-binding and osteogenic activity are promising functional ingredients for osteoporosis intervention. This study aims to explore whether a novel phosvitin phosphopeptide (Glu-Asp-Asp-pSer-pSer, EDDpSpS) with excellent calcium binding ability can promote the osteogenic differentiation of MC3T3-E1 cells and its potential mechanism. Network pharmacology analysis of EDDpSpS regulating osteoblast differentiation identified that the focal adhesion was a key signaling pathway, which is closely related to cell differentiation. Molecular simulations demonstrated that EDDpSpS was strongly bound to the target proteins within the focal adhesion signaling pathway and maintains a stable state. In addition, EDDpSpS had the potential to activate the key target protein Src tyrosine kinase (Src). Results of cell experiments showed that EDDpSpS (> 100 μg/mL) significantly promoted the differentiation and mineralization of MC3T3-E1 cells. Transcriptome analysis verified that the expression of paxillin (PXN), Src, actin beta/gamma 1 (ACTG1), and other osteoblast genes were regulated by EDDpSpS, leading to an up-regulation of the focal adhesion. In this study, the effect and the potential mechanism of EDDpSpS on osteoblast differentiation were clarified. EDDpSpS may be used as an effective dual-effect food ingredient for developing functional foods targeting osteoporosis.
研究领域:
营养学
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分子生物学
·
细胞生物学
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DOI
高影响因子
NPG Asia Materials
·
2018
·Vol. 10
·期 3
·e472
·
IF 7.208
Chen Zhonghui,Lv Ziyu,Wang Xue,Yang Hong,Qing Guangyan,Sun Taolei
Increasing evidence has indicated that glycans and their interactions with glycan-specific binding proteins have crucial roles in most physiological processes. One emerging topic in biomaterials is the construction of smart materials capable of recognizing and responding to these valuable glycans. However, because of the complicated compositions and structures as well as the low abundances and microheterogeneity of glycans, developing glycan-responsive materials with a high sensitivity and specificity is a long-term, challenging goal. Here, we report a biomimetic polymer capable of capturing and discriminating sialo-complex-type glycans with the core Neu5Ac-Gal-GlcNAc-Man sequence, which is one of the most effective biomarkers for cancer detection. As an optimized dipeptide, L-Asp-L-Phe has a strong but differential binding affinity for N -acetyl-neuraminic acid (Neu5Ac), N -acetyl-glucosamine (GlcNAc), galactose (Gal), and mannose (Man), which are the core construction units of sialylated glycans. In addition, the L-Asp-L-Phe-grafted polyethyleneimine film displays remarkable adsorption behavior for the model sialylated glycans and can discriminate their linkage isomers, which is accompanied by significant changes in the surface morphology and stiffness. These features facilitate the highly selective capture of sialylated glycopeptides from complex protein samples using PEI- g -L-Asp-L-Phe-modified mSiO 2 @SiO 2 @Fe 3 O 4 core-shell microspheres. The mechanism analysis demonstrates that the favorable polymeric spatial structures and multiple synergetic hydrogen-bonding interactions among the dipeptides and glycan are the main driving forces, indicating a clear direction for designing glycan-specific biomaterials.
研究领域:
生物材料学
·
分子生物学
·
医学工程
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产品关联
DOI
高影响因子
Frontiers in Chemistry
·
2022
·
IF 5.545
Qiao Sanyuan,Liu Qingwang,Fan Zhenzhong,Tong Qilei,Cai Li,Fu Yuanfeng
With the continuous improvement in oilfield development and the application of tertiary oil recovery technology, the water content of oilfield-produced fluids has gradually increased, and a large number of oilfield sewage with complex components has also been produced after oil–water separation, and effective treatment is urgently needed. ASP flooding sewage contains alkali, various surfactants, polymers, microemulsion oil droplets, and solid impurities, which are difficult to be effectively treated by traditional water treatment agents and methods. In this study, aminopropyl triethoxysilane (APTES) was used to modify the nano-Fe3O4 coated with tetraethyl silicate (TEOS). The product was used as the ferromagnetic nano-core for the iterative reaction of Michael addition and ester amidation to synthesize a magnetic hyperbranched polyamide amine, and its performance in the treatment of ASP flooding wastewater was evaluated experimentally. For the preparation of APTES-modified Fe3O4@SiO2 (FOSN) product, TEOS was coated over Fe3O4 in an ethanol aqueous solution environment and then APTES was added dropwise. The first-generation branched product (1-FSMN) was obtained by the reaction of FOSN and methyl acrylate graft product (FOSN-M) with ethylenediamine, and the highest yield was 93.7%. The highest yield of the second-generation branched product (2-FSMN) was 91.6%. In this study, a composite flooding wastewater sample from a block in the Bohai oilfield was taken. The suspended solids content was 143 mg/L, the oil content was 921.09 mg/L, the turbidity was 135 NTU, and the zeta potential was −47 mV. The third-generation hyperbranched polymer (3-FSMN) and its quaternary ammonium salt (3-FSMN-Q) performed best in the appropriate dosage range, with the highest oil removal rate of 97%, suspended solid removal rate of 90.3%, turbidity reduction rate of 86.6% and zeta potential reduction rate of 88%. For 3-FSMN and its quaternary ammonium salt, the gravity/magnetic PAC compound treatment experiment was carried out. In the settlement time of only 5 min, 3-FSMN/PAC and 3-FSMN-Q/PAC can achieve the maximum oil removal rate of 87.1% and suspended solids removal rate of 87.3% for polymer containing wastewater from ASP flooding, and 86.3 and 86.0% for 120 mg/L. Its treatment capacity was much better than that of common treatment agent combination (CPAM/PAC).
研究领域:
环境科学
·
化学工程
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产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2026
·
IF 4.2
Xiang Qu,Kaixiang Liu,Penglei Sun,Hailan Cui,Jingchao Chen,Haiyan Yu,Chaoxian Zhang,Xiangju Li
BACKGROUND Chinese sprangletop ( Leptochloa chinensis (L.) Nees), a dominant weed species in Chinese rice fields, poses a significant threat to rice yield and quality because of its increasing resistance to herbicides. In particular, resistance to cyhalofop-butyl (Cyb) has become a major concern in weed control. The mechanism by which the Asp-2078-Gly mutation confers resistance to acetyl-CoA carboxylase (ACCase) inhibitors in L . chinensis remains unclear. In this study, we collected Cyb-resistant L . chinensis populations from rice-growing regions and characterized their resistance mechanisms. RESULTS Through molecular analysis, we identified a resistant population harboring the Asp-2078-Gly mutation. Whole-plant dose–response experiments showed that the resistant (R) population exhibited varying levels of resistance compared with the susceptible (S) population: resistance indices values were 15.42 for FOP herbicides Cyb, 12.77 for metamifop, 11.50 for fenoxaprop-P-ethyl; 13.13 for the DIM herbicide clethodim; and 8.61 for the DEN herbicide pinoxaden. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis revealed no significant difference in ACCase gene expression between the Asp-2078-Gly mutant and the susceptible (S) population, indicating that resistance to Cyb was not due to target enzyme overexpression. Furthermore, the resistance of the mutant was not alleviated by P450 or glutathione S -transferase inhibitors, suggesting non-metabolic mechanisms. CONCLUSION Structural analysis confirmed that the Asp-2078-Gly mutation induces significant conformational changes in the herbicide-binding pocket, which reduces herbicide sensitivity and confers resistance. This study elucidates the resistance mechanism conferred by the Asp-2078-Gly mutation in L . chinensis , providing valuable insights for managing herbicide-resistant weeds. © 2026 Society of Chemical Industry.
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DOI
PubMed
高影响因子
MOLECULES
·
2026
·Vol. 31
·期 11
·1816
·
IF 5.1
Sanyuan Qiao,Luoqi Cui,Li Cai,Zhenzhong Fan
ASP flooding wastewater contains crude oil, suspended solids, anionic polymers and surfactants, with high viscosity, high zeta potential, difficult demulsification, flocculation and slow separation and sedimentation. In order to solve the problem of wastewater treatment of ASP flooding in oil fields, a magnetic branched core was prepared from ethyl silicate (TEOS), nano Fe3O4and aminopropyl triethoxysilane (APTES), and then reacted with polyamine and methyl acrylate to synthesize the magnetic hyperbranched molecule FSNMN with demulsification ability. Using acrylamide (AM), acryloxyethyl trimethylammonium chloride (DAC) and maleic anhydride (MA) as raw materials, cationic polymer long chain (CAMHA) with flocculating properties was synthesized and grafted with hyperbranched molecules. The demulsification flocculation ability of the product regarding ASP flooding wastewater was evaluated, and the demulsification flocculation mechanism was summarized. The results showed that the average molecular weight of 3-FSNMN4-C was 4.7 million, the cationic degree was 20.5%, and the saturation magnetization was 20 EMU/g. The removal rate of oil and suspended solids was 93.82% and 91.95% respectively when the simulated sewage was treated by magnetic field for 30 min. Magnetic hyperbranched star chain polymer provides a solution to the serious ecological environment problems caused by ASP flooding.
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）