ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Bo Wang,Linjie Wang,Xinyu Chen,Zheng Tang,Jinjin Liu,Shuangquan Liu,Xi Chen,Hao Liang,Xiangheng Niu
Probing the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) is of significance for clinical diagnosis of hepatic diseases and monitoring of therapeutic effects. Traditional detection methods often rely on a single signal driven by cascade catalysis of multiple natural enzymes, exposing weaknesses, including poor robustness and limited reliability. To overcome the deficiencies, here we design a novel nanoprobe (Na2V6O16/Cu-MOF) with both peroxidase-like catalytic and luminescent properties and establish a colorimetric/fluorescence bimodal method for the on-demand sensing of hepatic ALT and AST. In the bifunctional nanozyme, Na2V6O16 exhibits reposeful enzyme-like activity to trigger the oxidation chromogenic process of 3,3′,5,5′-tetramethylbenzidine (TMB) via H2O2-dependent free radical formation, and the 2-aminoterephthalic acid-coordinated Cu-MOF presents stable luminescence, which can be quenched by blue oxTMB from nanozyme catalysis via the inner filter effect. The presence of ALT or AST mediates the generation of H2O2, which consequently modulates the colorimetric turn-on and fluorescence readouts. With the enzyme–nanozyme cascade catalysis facilitated by Na2V6O16/Cu-MOF as well as its fluorescent feature, high-sensitivity bimodal detection of ALT and AST was obtained. Importantly, the established system offers robust catalytic performance, and the on-demand readouts enable mutual verification of different responses, both of which jointly improve the dependability of target analysis. Our work provides a reliable strategy for transaminase biomarker detection, contributing new insights into the design of multifunctional nanozymes and their applications in constructing advanced catalytic sensing platforms.
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高影响因子
SOLID STATE SCIENCES
·
2026
·
IF 3.6
Yingxia Ma,Shuo Zhou,Tianze Li,Miaoshi Li,Wenhui Han
A superparamagnetic Fe 3 O 4 @Zn-AST nanocomposite was constructed. • The Fe 3 O 4 @Zn-AST had abundant N, S-containing functional groups. • The Fe 3 O 4 @Zn-AST exhibits a saturation magnetization of 61.29 emu/g. • The maximum adsorption capacity of Fe 3 O 4 @Zn-AST for Ag(I) was 1410.68 mg/g. Silver as a precious metal, is extensively utilized in jewelry, electronics and some other fields due to its excellent conductivity and ductility. With the growing demand for silver, excessive exploitation of silver ore resources has generated large volumes of Ag(I)-containing wastewater. The direct release of untreated Ag(I)-containing wastewater contaminates ecosystems and poses risks to human health. Metal-Organic Frameworks (MOFs) exhibit a notably specific surface area, ample unsaturated metal sites, ease of functionalization, and tunable pore size and structure, which make them highly promising materials for effective adsorption of pollutants from wastewater. However, pure MOFs are typically in powder form, which are not easy to separate and recover after adsorption. In this work, using the zinc nitrate hexahydrate as the metal precursor along with 2-amino-5-sulfanyl-1,3,4-thiadiazole (AST) as the multi-dentate organic ligand, a superparamagnetic Fe 3 O 4 @Zn-AST nanocomposite was prepared via in-situ solvothermal method. The Fe 3 O 4 @Zn-AST exhibits a saturation magnetization of 61.29 emu/g, meeting the requirements for magnetic separation. The maximum Ag(I) adsorption capacity of Fe 3 O 4 @Zn-AST is 1410.68 mg/g, and adsorption process is mainly chemical adsorption, featured by endothermic, disordered, and spontaneous. Adsorption-desorption experiments prove that the removal efficiency can remain above 91% even after five cycles. From FT-IR and XPS, the Fe 3 O 4 @Zn-AST adsorbing Ag(I) was mainly through the synergistic effect of -SH, N-containing functional groups and electrostatic interactions. Given the magnetic separation advantage and superior Ag(I) adsorption, Fe 3 O 4 @Zn-AST could serve as a promising adsorbent. Download: Download high-res image (241KB) Download: Download full-size image
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高影响因子
CURRENT TOPICS IN MEDICINAL CHEMISTRY
·
2024
·
IF 2.9
Su Hongbing,Lei Hong,Cao Ling,Zhou Xiaoying,Liu Yumeng,Li Ying,Yan Lili,Shaowa Lv,Wang Yanhong,Guan Qingxia
Background: Self-emulsifying nano-phase of traditional Chinese medicine are a research
hotspot. Xiao-Chai-Hu decoction is a commonly used compound decoction in clinical practice,
which is of great research significance. The aim of this study was to isolate and characterize
the self-emulsifying nano-phase and other phases of Xiao-Chai-Hu decoction, and to study the effects
of each phase on acute liver injury.
Methods: The liquid medicine was prepared employing centrifugation followed by dialysis. Single-
factor investigation methodology was utilized to optimize the preparation parameters for both
phases. Characterization of the formulated phase involved analyses such as surface morphology assessment,
measurement of nanoparticle size and Zeta potential using an analyzer, observation of
the Tyndall effect, conducting diffusion and dilution tests, examination under a microscope, and
structural visualization via transmission electron microscopy (TEM). Furthermore, an acute liver
injury model was established in rats through intraperitoneal injection of D-Galactosamine (D-Gal-
N). To assess hepatic function and oxidative stress status, serum levels of alanine aminotransferase
(ALT) and aspartate aminotransferase (AST), superoxide dismutase (SOD) activity, and
malondialdehyde (MDA) content in liver tissue were quantified. The liver coefficients for each
group were calculated as an additional parameter. For histopathological evaluation, liver tissue sections
from the experimental group were stained with Hematoxylin and Eosin (H&E) and examined
microscopically under light conditions. These revisions aim to enhance clarity, correct minor
grammatical errors (such as capitalization of “HE” to “H&E”), and ensure a smoother flow of
information without altering the scientific content of your original text.
Results: Successful establishment and separation of four distinct phases were achieved, including
the self-emulsifying nano-phase, precipitation phase, suspension phase, and true solution phase.
The self-emulsifying nano-phase was characterized as spherical particles with an average diameter
of approximately 100 nm. Pharmacodynamic assessments revealed that both Xiao-Chai-Hu decoction
and its self-emulsifying nano-phase significantly reduced liver coefficients and alanine aminotransferase
(ALT) levels compared to controls (P<0.05). However, no statistically significant differences
were observed in regards to aspartate aminotransferase (AST) concentrations, malondialdehyde
(MDA) content, or superoxide dismutase (SOD) activity between the treatment groups
and control (P>0.05). These findings indicate that both Xiao-Chai-Hu decoction and its self-emulsifying
nano-formulation ameliorated D-GalN-induced acute liver injury, albeit without statistically
distinguishable efficacy between them (P>0.05).
Conclusion: The presence of a self-emulsifying nano-phase within Xiao-Chai-Hu decoction is
confirmed, and this nano-phase emerges as a therapeutically efficacious component in mitigating
acute liver injury.
研究领域:
中药学
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药理学
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2015
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IF 2.858
Guangpu Liu,Xinquan Liu,Yongchun Zhang,Fang Zhang,Ting Wei,Min Yang,Kaiming Wang,Yongjie Wang,Na Liu,Huiling Cheng,Zhongxi Zhao
Jujube polysaccharides have been proved to have various bioactivities. This study was designed to evaluate the chemical composition and hepatoprotective function of the polysaccharides extracted from Zizyphus jujube cv. Huanghetanzao (HJP). The composition of HJP was determined as heteropolysaccharides with galactose and arabinose being the main components. The pretreatment of mice with HJP significantly ( p < 0.01) reduced the activities of serum hepatic AST, ALT, and LDH induced by CCl 4 or acetaminophen (APAP) while the commercial liver-injury treatment drug silybin did not show any prevention effects. Mechanistic study results indicate that the administration of the CCl 4 - or APAP-injured mice with HJP enhanced SOD and GSH-Px and decreased MDA, indicating that anti-oxidation and detoxification could be the pathways for the liver protection observed. In addition, the liver prevention and treatment effects of HJP on the liver damage induced by CCl 4 or APAP obtained from the liver enzyme analyses were confirmed by the hepatic histopathology studies in mice. Therefore, HJP could be used as a prevention and treatment agent for liver injury induced by liver toxic chemicals and drugs.
研究领域:
食品科学与工程
·
医学
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PubMed
高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2025
·
IF 3.5
Yingxia Ma,Miaoshi Li,Tianze Li,Haijun Yang,Ruilin Zhang,Xiaofei Ye,Wenli Meng,Xiaojun Chai,Cuixia Li
Industrial wastewater containing heavy metal Ag(I) causes irreversible harm to human health through food chain, necessitating effective removal methods. According to hard-soft acids-bases (HSAB) theory, nitrogen (N) and sulfur (S) functional groups form stable chelates with Ag(I). In this study, 2-amino-5-sulfanyl-1,3,4-thiadiazole (AST) with three N and two S groups served as organic ligand, and zinc nitrate hexahydrate provided metal center, novel amorphous metal-organic framework (Zn-AST) rich in N and S groups was synthesized via a one-pot solvothermal method. Batch experiments assessed the Zn-AST adsorption properties for Ag(I) in aqueous solutions. In addition, density-functional theory, frontier orbital theory, and molecular electrostatic surface potential analyses were used to elucidate the synthesis and adsorption mechanisms. The maximum adsorption capacity of Zn-AST for Ag(I) was 2932.91 mg/g. The Zn-AST exhibited good selectivity in the presence of Cu(II), Co(II), Ni(II), and Pb(II) ions, the removal rate for Ag(I) was 98.28 %, which was far higher than the values of other ions (all less than 10 %). The main adsorption mechanisms of Ag(I) by the Zn-AST involved electrostatic interactions, the formation of Ag–S bonds, and chelation between Ag and nitrogen functional groups. This study offers a viable strategy for developing unique adsorbents for Ag(I) removal from wastewater.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
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IF 6.1
Tongwen Huang,Ke Zhang,Junqiao Wang,Kaihong He,Xingtao Zhou,Shaoping Nie
Acrylamide (ACR) generated in carbohydrate-rich foods during thermal processing has been demonstrated to exhibit hepatotoxicity. As one of the most consumed flavonoids with diet, quercetin (QCT) possesses the ability to protect against ACR-induced toxicity, albeit its mechanism is unclear. Herein, we discovered that QCT alleviated ACR-induced elevated levels of reactive oxygen species (ROS), AST, and ALT in mice. RNA-seq analysis revealed that QCT reversed the ferroptosis signaling pathway upregulated by ACR. Subsequently, experiments indicated that QCT inhibited ACR-induced ferroptosis through the reduction of oxidative stress. With autophagy inhibitor chloroquine, we further confirmed that QCT suppressed ACR-induced ferroptosis by inhibiting oxidative stress-driven autophagy. Additionally, QCT specifically reacted with autophagic cargo receptor NCOA4, blocked the degradation of iron storage protein FTH1, and eventually downregulated the intracellular iron levels and the consequent ferroptosis. Collectively, our results presented a unique approach to alleviate ACR-induced liver injury by targeting ferroptosis with QCT.
研究领域:
毒理学
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药理学
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分子生物学
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细胞生物学
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PubMed
高影响因子
EXPERIMENTAL PARASITOLOGY
·
2016
·
IF 1.623
Xiaochuan Zhang,Lili Jin,Zhe Cui,Changhao Zhang,Xue Wu,Hyun Park,Hongmei Quan,Chunmei Jin
Toxoplasma gondii ( T . gondii ) is an important pathogen which can causes serious public health problems. Since the current therapeutic drugs for toxoplasmosis present serious host toxicity, research on effective and new substances of relatively low toxicity is urgently needed. This study was carried out to evaluate the anti-parasitic effect of oxymatrine (OM) and matrine (ME) against T . gondii in vitro and in vivo . In our study, the anti- T . gondii activities of ME and OM were evaluated in vitro using cell counting kit-8 assay, morphological observation and trypan blue exclusion assay. In vivo , mice were sacrificed four days post-infection and ascites were drawn out to determine the extent of tachyzoite proliferation. Viscera indexes and liver biochemical parameters, such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), glutathione (GSH) and malondialdehyde (MDA), were examined to evaluate the toxicity of compounds to mice. As a result, OM and ME showed anti- T . gondii activity but low selectivity toxicity to HeLa cells. Both compounds also significantly decreased the number of tachyzoites in peritoneal cavity and recovered the levels of ALT, AST, GSH and MDA in liver. Moreover, the mice treated with OM or ME achieved better results in viscera index and survival rate than that of spiramycin. These results suggest that OM and ME are likely the sources of new drugs for toxoplasmosis, and further studies will be necessary to compare the efficacy of drug combination, as well as identify its action of mechanism.
研究领域:
寄生虫学
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药理学
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传染病学
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高影响因子
JOURNAL OF APPLIED MICROBIOLOGY
·
2019
·Vol. 127
·期 6
·1716-1726
·
IF 2.683
Cheng R.,Wang L.,Li J.,Fu R.,Wang S.,Zhang J.
AimsTo purify and characterize an exopolysaccharide (EPS) from an Agrobacterium strain ZCC3656 with high EPS‐secreting performance and investigate its anti‐inflammatory activity using lipopolysaccharide (LPS)‐induced macrophage cells in an acute liver injury mouse model.Methods and ResultsTwelve rhizobial strains were compared for EPS fermentation production in modified M9 salts supplemented with mannitol or sucrose as the sole carbon source. Agrobacterium sp. ZCC3656 exhibited the highest EPS yield (21·1 g l−1) and was characterized for EPS production by carbon source utilization, time course fermentation and serial subcultivation assays. The EPS, designated Riclin, was purified by deproteinization using the Sevag method. The combined results of gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses indicated that Riclin is a succinoglycan‐like polysaccharide comprised of glucose, galactose, succinate and pyruvate at a ratio of 7·8. : 1·0 : 0·9 : 1·1 and has an molecular weight of approximately 2·5 × 106 Da. Riclin inhibited TNF‐α, IL‐1β and IL‐6 expression in LPS‐stimulated RAW 264.7 macrophage cells in a dose‐dependent manner. In addition, Riclin pretreatment increased the survival rate of D‐Gal/LPS treated mice, inhibited serum ALT and AST activities and reduced the production of the inflammatory mediators TNF‐α, IL‐1β and IL‐6.ConclusionsAgrobacterium sp. ZCC3656 is a highly stable EPS‐producing strain. The EPS Riclin from ZCC3656 is a succinoglycan‐type polysaccharide that is noncytotoxic and exhibits remarkable anti‐inflammatory effects in vivo and in vitro.Significance and Impact of the StudySuccinoglycans are well known for good rheological properties and their physiological interactions with plants. However, their potential activity towards mammals has received little attention. Our study revealed that the succinoglycan Riclin exhibited excellent anti‐inflammatory activities and could be considered as a promising reagent in anti‐inflammatory treatment.
研究领域:
微生物学
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免疫学
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药理学
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PubMed
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2018
·
IF 3.457
Li Zhang,Hui-Xia Li,Wu-Si Pan,Farhan Ullah Khan,Cheng Qian,Feng-Rong Qi-Li,Xiaojun Xu
Background and aims Non-alcoholic steatohepatitis (NASH) is the hepatic manifestation of metabolic syndrome and is characterized by steatosis, inflammation, and fibrosis. We aim to characterize the hepatoprotective effects of Leonurine hydrochloride (LH) and the possible pathway in a cell and rodent model of diet-induced steatohepatitis (NASH). Methods For in vitro studies, Palmitic acid (PA) and free fatty acid (FFA) induced HepG2 and HL7702 steatosis cell models were used. For in vivo studies, NASH was induced by feeding mice MCD diet. These mice received either placebo or LH at three different doses (50、100、200 mg/kg/day) for 6 weeks. Histological staining’s, and commercially available kits for ALT and AST and hepatic contents of TG, TC, MDA, SOD, and GSH were used to assess NASH. Furthermore, relative liver protein and gene expression levels were determined by Western Blot and qPCR, respectively. Results After establishing NASH models, LH treatment improved lipid accumulation, hepatic contents of TG, TC, and expression levels of ALT and AST in dose-dependent manner. Also, LH improved MDA, SOD, and GSH expression levels. The results of RT-PCR and Western blotting showed that LH upregulated the expression of AMPK phosphorylation and downregulated SREBP-1c and its target genes expression level. Conclusions Our data reveal the promising role of Leonurine hydrochloride in the prevention and treatment of NASH, in vitro and in vivo . This effect may be partially mediated by the AMPK/SREBP1 pathway. These findings provide a novel therapeutic target for the clinical treatment of NASH.
研究领域:
药理学
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肝病学
·
代谢性疾病
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PubMed
高影响因子
Frontiers in Nutrition
·
2022
·
IF 6.59
Han Mengyao,Zhang Zhongshan,Li Xinyue,Tong Haibin,Xu Zhiguo,Ding Zikang,Yang Anquan,Xie Min,Wang Xiaomei
To investigate the protective effect of collagen peptide from Micropterus salmoides skin (CPMs) on oxidative damage induced by cyclophosphamide in mice. Balb/c female mice were divided into blank, model (cyclophosphamide, CTX), positive control (levamisole hydrochloride), and collagen peptide low-, medium-, and high-dose groups. The results showed that CPMs increase the body mass and immune-related organ indexes, such as liver and kidneys of immunosuppressed mice. The activities of ALT, AST, UA, BUN, and MDA in the liver and kidney tissues decreased significantly, while those of SOD and GSH-Px increased significantly. CPMs can relieve the pathological damage to immune organs. CPMs significantly increase the activities of IL-2, IgG, and TNF-α in serum and SOD activity, while the MDA content was decreased compared to the model group. CPMs can exert a protective effect on cyclophosphamide-induced oxidative damage and have application prospects in the field of health food.
研究领域:
药理学
·
免疫学
·
毒理学
·
营养学
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PubMed
高影响因子
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
·
2021
·Vol. 99
·期 12
·
IF 2.273
Weimin Xu,Peng Wang,Dalong Wang,Ke Liu,Shuaishuai Zhang,Wei Zhao,Guoqing Liu
The aim of the present study was to investigate the protective effect of S-ketamine (S-KET) against carbon tetrachloride (CCl4) – induced liver damage and oxidative stress, as well as to elucidate the related underlying mechanisms. Blood was collected to measure biochemical parameters (alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), total bilirubin (TB) and γ-glutamyltransferase (γ-GT)) and the liver was harvested for histopathological analysis of enzymes related to the antioxidant response (malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), and glutathione peroxidase (GSH-PX)). Liver cell apoptosis was evaluated using the TUNEL assay. In addition, the expression levels of apoptosis-related proteins and the nuclear factor erythroid 2–related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway were detected by Western blot analysis to explore potential mechanisms. S-KET protected the liver from CCl4-induced damage. The changes to the liver biochemical parameters (increased ALT, AST, ALP, TB, and γ-GT) and oxidative stress-related indicators (increased MDA; depleted SOD, GSH, and GSH-PX) induced by CCl4 were inhibited by S-KET. S-Ket also inhibited CCl4-induced cell apoptosis, the changes in expression of related proteins, and blocked CCl4-induced liver injury and oxidative stress via activation of the Nrf2/HO-1 signaling pathway. S-KET effectively protected the liver by inhibition of CCl4-induced damage via upregulation the Nrf2/HO-1 signaling pathway.
研究领域:
药理学
·
肝病学
·
分子生物学
·
毒理学
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DOI
PubMed
高影响因子
PHYTOMEDICINE
·
2022
·
IF 6.656
Zixia Hu,Yanjia Zhao,Yi Yang,Wei Li,Rong Tan,Linhua Zhao,Xiaolin Tong,Ying Peng,Jiang Zheng
Background Dioscorea bulbifera L. (DBL) is a common herbal medicine where furanoterpenoid diosbulbin B (DSB) is a major component responsible for its hepatotoxicity . The metabolic oxidation of the furan moiety of DSB, resulting in covalent binding to hepatic protein , is considered to initiate its liver injury. Purpose We aimed to develop a mechanism-based plasma protein adduction-based biomarker to determine DBL exposure and to predict the onset of hepatotoxicity induced by DBL. Methods Rats were intragastrically treated with DBL extract, and the plasma samples were collected. Plasma ALT and AST were measured with commercial kits. Plasma protein modification was determined by immunoblot assay. Assessment of DSB-induced protein adduction was achieved by LC-MS/MS analysis of complete proteolytic digestion of adducted protein to pyrroline derivative A4 using pronase enzyme . The structure of the resulting pyrroline derivatives was confirmed by NMR. Results Plasma protein of rats treated with DBL extract was covalently modified by the metabolite of DSB. Pyrroline derivative A4 was detected in proteolytic digestion of plasma obtained from rats administered DBL extract. The protein adduction elevated with the increase in the dosage of DBL extract. A detectable level of plasma was observed 10 days after withdrawal of DBL extract post 30-day continuous administration. In addition, the elevation trend of plasma ALT was found to be proportional to the accumulation trend of pyrroline derivative A4. Conclusion DSB-derived plasma protein adduction correlated well with the exposure of DBL in rats. The protein adduction may be used as a good biomarker for diagnosis of DBL-induced liver injury and a useful indicator for DBL medication plans.
研究领域:
药理学
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毒理学
·
分析化学
·
分子生物学
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PubMed
高影响因子
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