JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Xiaojiao Dai,Ziling Huang,Chuanyi Liu,Zhigang Li,Qingmin Chen,Yi Xu,Xiaomei Chen,Quansheng Chen,Jie Wei
Adenosine triphosphate (ATP) levels are recognized as a critical indicator of microbial content in food safety. The development of highly efficient ATP-sensing methods is essential for assessing food freshness. Herein, we propose a simple-yet-efficient hemin/G-quadruplex (G4) DNAzyme-based aptasensor for ATP detection and oyster freshness assessment. The anti-ATP aptamer subunits specifically bind to ATP, leading to self-assembly of the hemin/G4 DNAzyme. The newly produced DNAzyme then catalyzes the oxidation of ABTS 2- to produce the colored ABTS •− , providing an absorbance response for sensitive ATP analysis. The aptasensor facilitates high-performance and accurate determination of ATP with a low detection limit of 0.065 μM. Furthermore, this sensor enables reliable ATP analysis in oyster samples and evaluation of oyster freshness. In particular, a good linear correlation was established between microbial content and ATP levels. The proposed aptasensor for the assessment of freshness is characterized by its simplicity and efficiency, potentially offering a new tool to monitor food safety.
研究领域:
生物化学
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食品安全
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分子生物学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 7
·1-11
·
IF 5.3
Liang Pengda,Lv Bei,Chen Ke,Li Dawei
The combination of CRISPR/Cas12a and functional DNA provides the possibility of constructing biosensors for detecting non-nucleic-acid targets. In the current study, the duplex protospacer adjacent motif (PAM) in the activator of CRISPR/Cas12a was used as a molecular switch, and a sensitive adenosine triphosphate (ATP) detection biosensor was constructed using an allosteric probe-conjugated PAM site formation in hybridization chain reaction (HCR) integrated with the CRISPR/Cas12a system (APF-CRISPR). In the absence of ATP, an aptamer-containing probe (AP) is in a stem-loop structure, which blocks the initiation of HCR. In the presence of ATP, the structure of AP is changed upon ATP binding, resulting in the release of the HCR trigger strand and the production of long duplex DNA with many PAM sites. Since the presence of a duplex PAM site is crucial for triggering the cleavage activity of CRISPR/Cas12a, the ATP-dependent formation of the PAM site in HCR products can initiate the FQ-reporter cleavage, allowing ATP quantification by measuring the fluorescent signals. By optimizing the sequence elements and detection conditions, the aptasensor demonstrated superior detection performance. The limit of detection (LOD) of the assay was estimated to be 1.16 nM, where the standard deviation of the blank was calculated based on six repeated measurements. The dynamic range of the detection was 25–750 nM, and the whole workflow of the assay was approximately 60 min. In addition, the reliability and practicability of the aptasensor were validated by comparing it with a commercially available chemiluminescence kit for ATP detection in serum. Due to its high sensitivity, specificity, and reliable performance, the APF-CRISPR holds great potential in bioanalytical studies for ATP detection. In addition, we have provided a proof-of-principle for constructing a CRISPR/Cas12a-based aptasensor, in which the PAM is utilized to regulate Cas12a cleavage activity. Graphical Abstract
研究领域:
生物化学
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分子生物学
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分析化学
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DOI
PubMed
高影响因子
TALANTA
·
2017
·
IF 4.162
Fangfang Li,Xuan Hu,Fengyi Wang,Baozhan Zheng,Juan Du,Dan Xiao
A simple, rapid, and ultrasensitive fluorescence strategy for adenosine triphosphate (ATP) detection was developed by using a FAM (carboxyfluorescein) labeled DNA (FAM-DNA). In this strategy, highly fluorescent FAM-DNA was used as a probe, and nanoceria (CeO 2 NPs) acted as an efficient quencher. FAM-DNA attached to the surface of nanoceria through the coordination between the phosphate group of DNA and NP surface, which induced complete quenching in the FAM-DNA fluorescence due to a photo induced electron transfer (PET) process. It was found that ATP can readily displace adsorbed DNA from nanoceria surface because of the stronger coordination ability of ATP with nanoceria, and the nanoceria-based competitive binding resulted in over 7-fold fluorescence enhancement. Over a wide range from 0.1 nM to 1.5 μM, a good linear relationship between the fluorescence intensity and the concentration of ATP was obtained and the detection limit was estimated to be as low as 54 pM. This method was successfully used to analyze ATP in a single drop of blood and human urine.
研究领域:
生物化学
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分析化学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Wei Wan,Meijia Liu,Xiangling Ren,Longfei Tan,Changhui Fu,Qiong Wu,Jun Ren,Hongbo Li,Zhongbing Huang,Xianwei Meng
Adenosine triphosphate (ATP) actively involve into cellular processes. Abnormal levels of ATP related to a variety of diseases, therefore determining the ATP level is of pivotal significance for diagnoses. Here, a highly sensitive fluorescence probe based on gold nanoclusters (Au NCs) was constructed for the detection of ATP, and an expanded detection range and low limit of detection (LOD) were realized in our work. Zeolitic imidazolate framework-8 (ZIF-8) enhanced the fluorescence intensity of Au NCs by the encapsulation and less ATP cause to a stronger fluorescence change, which guaranteed detection reproducibility and facilitated lower LOD. The LOD of Au NCs encapsulated ZIF-8 (AZ) for ATP detection was expanded to 2 μM, which was only 0.05 % of sole Au NCs (4000 μM). Significantly, ATP of high concentration quenched AZ by disrupting the structure of ZIF-8 which expanded the detection range. Moreover, the linear range of high concentration ATP detection in serum was 600–4000 μM, which was closed to the normal level of ATP in physiological. Therefore, due to its low LOD and high sensitivity, AZ can be applied for fluorescence detection and initial diagnosis of disease related to ATP.
研究领域:
生物化学
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医学诊断
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材料科学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Lingchen Wang,Yue Huang,Linlin Sun,Xiaojuan Gong,Chuan Dong,Shaomin Shuang
Herein, a novel rapid, specific, and sensitive probe for adenosine triphosphate (ATP) detection is developed. We have pioneered the integration of split ATP aptamers with aggregation-induced emission (AIE) via chemical binding to obtain a novel ATP detection probe (TPE probe). Upon the binding of the ATP split aptamer to the target, the intermolecular distance of the AIE molecules decreases, restricting their spatial rotation, which leads to an enhancement of the fluorescence signal, thereby enabling the detection of ATP. This unique design provides the probe with both target-specific recognition capability of the aptamer and sensitive signal-reporting function. Under the optimized experimental conditions, the probe exhibits a linearity range from 1-1000 nM with limit of detection 0.14 nM, displaying a comparative advantage over previously reported methods in terms of sensitivity and selectivity. Additionally, this method demonstrated satisfactory performance for ATP detection in beer samples, with recoveries between 97.5 % to 104.6 %. More importantly, the current research is expected to provide an effective monitoring method for beer quality control.
研究领域:
生物化学
·
分析化学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Xin Xu,Danyang Nan,Huan Yang,Shuang Pan,Hui Liu,Xiaoli Hu
Quercetin is a nature polyphenolic flavonoid compound, which act as antioxidant and radical scavengers in cells. However, such activities strongly depend on the intracellular microenvironment, which has become an urgency to develop a more stable method for better use of quercetin. Herein, we report a facile approach to fabricate Quercetin@ZIF-90 (QZ) with a narrow size distribution about 100 nm, the synthesized QZ exhibits considerable antioxidant and radical scavenger ability comparing to bare quercetin. Moreover, the experiment results indicated that QZ was sensitive to adenosine 5′-triphosphate (ATP) and pH, thus QZ was applied as a novel antioxidant for the determination of ATP. In brief, though 3, 3′, 5, 5′-tetramethylbenzidine (TMB) could be oxidized to blue cation radical complex by oxidase-like BSA-MnO 2 nanosheets (MNs) at neutral pH, QZ could sufficiently protect against MNs-induced TMB cation radicals reaction, which means no obvious color change in MNs-TMB system after the addition of QZ. However, ATP could induced collapse of QZ due to the interaction between ATP and Zn 2+ allowing the release of quercetin from ZIF-90 shell, and the inhibition effect to the MNs was weakened, leading to the recovery of blue oxTMB. In this way, a novel “Off-On” colorimetric method for ATP sensing was established, and the method displayed a good linear relationship ranging from 2.0 μ mol L −1 to 80.0 μ mol L −1 . The reported strategy is also capable of ATP sensing in MCF-7 cell lysates, which illustrated our method exhibited high sensitivity and selectivity.
研究领域:
生物化学
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分析化学
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材料科学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2023
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IF 7.4
Xiaomeng Zhou,Saijin Huang,Dan Zhang,Wenfeng Liu,Wenxing Gao,Yumeng Xue,Li Shang
Adenosine triphosphate (ATP) participates in the regulation of most biological processes, and the ATP level is closely associated with many diseases. However, it still remains challenging to achieve on-site monitoring of ATP in an equipment-free and efficient way. Microneedles, a minimally invasive technology that can extract biomarkers from liquid biopsies, have recently emerged as useful tools for early diagnosis of a broad range of diseases. In this work, we developed hydrogel microneedles that are loaded with ATP-specific dual-emitting gold nanoclusters (RhE-AuNCs) for fast sampling and on-needle detection of ATP. These RhE-AuNCs were photo-crosslinked to the hydrogel matrix to form a fluorescent microneedle patch. Based on the ATP-induced Förster resonance energy transfer in RhE-AuNCs, a highly selective, sensitive, and reliable ATP sensor was developed. Moreover, simultaneous capture and visual detection of ATP was achieved by the AuNC-loaded microneedle sensing platform, which exhibits promising sensing performance. This work provides a new approach to design a point-of-care ATP sensing platform, which also holds great potential for the further development of microneedle-based analytical devices.
研究领域:
生物医学工程
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分析化学
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分子生物学
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DOI
PubMed
高影响因子
SENSORS
·
2018
·Vol. 18
·期 10
·3281
·
IF 2.475
Jingjing Zhang,Chunzheng Yang,Chaoqun Niu,Chen Liu,Xuepin Cai,Jie Du,Yong Chen
In this study, a label-free fluorescent, enzyme-free, simple, highly sensitive AND logic gate aptasensor was developed for the detection of adenosine triphosphate (ATP). Double-stranded deoxyribonucleic acid (DNA) with cohesive ends was attached to graphene oxide (GO) to form an aptasensor probe. ATP and single-stranded DNA were used as input signals. Fluorescence intensity of PicoGreen dye was used as an output signal. The biosensor-related performances, including the logic gate construction, reaction time, linearity, sensitivity, and specificity, were investigated and the results showed that an AND logic gate was successfully constructed. The ATP detection range was found to be 20 to 400 nM (R2= 0.9943) with limit of detection (LOD) of 142.6 pM, and the sensitivity range was 1.846 × 106to 2.988 × 106M−1. This method for the detection of ATP has the characteristics of being simple, low cost, and highly sensitive.Keywords:ATP detection;label-free fluorescence;enzyme-free;graphene oxide;logic gate
研究领域:
生物化学
·
分子生物学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2013
·
IF 5.437
Bingqian Liu,Bing Zhang,Guonan Chen,Huanghao Yang,Dianping Tang
A new flow-through electrochemical aptasensor was designed for ultrasensitive monitoring of adenosine triphosphate (ATP) by coupling microvalve-programmable capillary column with CdS-functionalized DNA concatamer for signal amplification . Initially, a layer of primary DNA-conjugated polyacrylamide hydrogel was covalently linked onto the internal surface of capillary column, and then an automated sequenctial injection format with a syringe pump was employed for development of the programmable capillary-based aptasensor. In the presence of target DNA aptamer, the immobilized primary DNA hybridized with partial bases of the aptamer. The excess aptamer fregment could trigger the formation of DNA concatamer between auxiliary DNA 1 and CdS-labeled auxiliary DNA 2 . Upon target ATP introduction, a specific ATP-aptamer reaction was excuated, thereby resulting in the release of CdS-functionalized DNA concatamer from the capillary. Subsenquent anodic stripping voltammetric detection of cadmium released under acidic conditions from the released CdS nanoparticles could be conducted in a homemade detection cell. Under optimal conditions, the dynamic concentration range spanned from 0.1 pM to 10 nM ATP with a detection limit of 0.06 pM ATP. The electrochemical aptasensor showed good reproducibility, selectivity, and stability. In addition, the methodology was evaluated for the analysis of ATP spiked serum samples, and the recoveries was 81–140%.
研究领域:
生物化学
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分析化学
·
分子生物学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
De-Yan Qi,Chong Wang,Yan-Cai Gao,Hong-Wei Li,Yuqing Wu
The glutathione-capped bimetallic copper and gold nanoclusters (CuAuNCs@GSH) were achieved through Au doping into the unstable copper nanoclusters (CuNCs@GSH), which induced the photoluminescence stabilization time increase from 4 to 7 days. Moreover, the Ce 3+ introduction further significantly increased the stable storage time (from 7 to 25 days) and the emission intensity (near 140-fold), accompanying a quantum yields enhancement from 0.30% to 22%. Transmission electron microscopy (TEM) revealed the Ce 3+ -induced larger assemblies of CuAuNCs-Ce 3+ from monodispersive CuAuNCs@GSH nanodots . The fluorescence enhancement was attributed to the aggregation-induced emission (AIE) property of CuAuNCs@GSH, where Ce 3+ was bound to the ligands GSH and reduced the non-radiative relation of the excited states. Besides, the strong interaction between ATP and Ce 3+ dissociated the CuAuNCs-Ce 3+ assembly into monodisperse nanoclusters and recovered the weak luminescence. Therefore, the CuAuNCs-Ce 3+ assembly was applied to determine ATP, which exhibited high selectivity and sensitivity with a detection limit of 53 nM. Finally, the method was successfully applied to determine ATP in diluted serum with very high recovery. Therefore, the present study provides an efficient way to improve the fluorescence properties of metal nanoclusters and supplies a selective and sensitive ATP sensor.
研究领域:
生物化学
·
分析化学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Jiaona Xu,Chunying Wei
Two general and reliable fluorescence sensors were proposed in this work utilizing aptamer DNA-templated silver nanoclusters (Ag NCs). Both DNA-AgNCs could be used for label-free detecting of ATP with the limits of detection of 0.44 and 0.65 mM. One of them was further applied to monitor the activity of adenosine deaminase (ADA). In our effort to elucidate the light-up mechanism, we studied a total of six Ag NCs prepared by different DNA sequences , and found that they showed different sensitivity to ATP. Both BT3T3- and BT3T3(R)-templated Ag NCs were chose to make particular studies by UV–vis, TEM, fluorescence, and TCSPC methods. The results showed that when DNA-Ag NCs was kept for 1.5 h and presented a strong fluorescence, the addition of ATP failed to cause a large change of fluorescence intensity ; on the contrary, after Ag NCs was kept for 24 h and emitted a weak fluorescence, adding ATP was able to result in the large fluorescence enhanced of 43 and 33 times for BT3T3- and BT3T3(R)-templated Ag NCs, respectively. The possible mechanism was also suggested that ATP binding to aptamer segment of template induced the change of the DNA secondary structure , which made the aggregated Ag nanoparticles disperse into Ag NCs with an average diameter of about 2 nm that were responsible for the large fluorescence increase. Moreover, ATP could protect the fluorescence intensity of BT3T3(R)-templated Ag NCs from quenching for at least 9 h.
研究领域:
生物化学
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分子生物学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Shicai Xu,Chao Zhang,Shouzhen Jiang,Guodong Hu,Xiaoyue Li,Yan Zou,Hanping Liu,Jun Li,Zhenhua Li,Xiaoxin Wang,Mingzhen Li,Jihua Wang
As the major energy molecule of cells, adenosine triphosphate (ATP) regulates various biological processes and has been found to be closely related to many diseases. Therefore, ATP detection in trace amounts is very useful for understanding various biological processes, studying cellular events such as proliferation and apoptosis, and estimating contaminated degree of food and medical instrument. To date, the trace sensing ATP at picomolar level in biological systems is still a major challenge. Because of unique electrical and structural properties, graphene has attracted much attention in biosensing applications. Here, a sensitive and selective graphene foam field-effect transistor (GF-FET) biosensor for ATP detection is demonstrated. The lowest detection limit of the biosensors for analyzing ATP is down to 0.5 pM, which is one or several orders lower than the reported results. Moreover, the GF-FET biosensor show a good linear current response to ATP concentrations in a broad range from 0.5 pM to 50 μM. The GF-FET sensor surface can be regenerated for many times and used for up to weeks without significant loss of functionality. Based on this sensing platform, label-free measurements of ATP concentrations in human serum as well as in cell lysate are demonstrated. The work may provide a novel platform to study ATP release and energy-regulated biological processes, suggesting a promising future for biosensing applications.
研究领域:
生物传感器
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材料科学
·
医学诊断
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）