SENSORS AND ACTUATORS B-CHEMICAL
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2020
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IF 7.1
Shun Hu,Xiaona Li,Kai Wang,Qiuhua Wu,Guolin Zhang,Xue Liu
A simple indicator displacement assay (IDA)-based array system was established by using Fe(III)-responsive fluorescent carbon dots (CDs) and Fe(III) to detect ATP-related physiological phosphates (PPs) and monitor ATP hydrolysis. The analysis was based on principal component analysis of differential CDs fluorescence regulated by Fe(III)/PPs complexes. By two modeling methods, two sensor arrays were established. That is, three CDs/one Fe(III) concentration (Array1) and one CD/four Fe(III) concentrations (Array2). Compared with Array1, Array2 saved a lot of trouble in preparing the CD sensors and the analytical performance it is hardly affected. The Array2 can distinguish PPs in serum environment and from interfering substances, such as amino acids and metal ions. Besides, quantitative analysis of ATP single-component system and qualitative analysis of ATP/ADP two-component system can be achieved. On this basis, ATP hydrolysis processes were monitored, and results suggested that ATP hydrolysis completed within 20 min under the catalysis of 24 U/mL apyrase. As ATP hydrolysis works for 30 min, its main products were mainly AMP and orthophosphate (Pi).
研究领域:
生物化学
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分析化学
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DOI
高影响因子
ANALYST
·
2018
·Vol. 143
·期 16
·3821-3828
·
IF 3.864
Chuanxia Chen,Qun Yuan,Pengjuan Ni,Yuanyuan Jiang,Zhenlu Zhao,Yizhong Lu
Alkaline phosphatase (ALP) is a significant biomarker for diagnostics. Simple, selective and sensitive detection of ALP activity is thus of critical importance. In this study, an artful fluorescence assay for ALP is proposed based on adenosine triphosphate (ATP) hydrolysis-triggered disassociation and fluorescence quenching of cerium coordination polymer nanoparticles (CPNs). ATP, a recognized natural substrate of phosphatase, can serve as a superb “antenna” to sensitize the luminescence of Ce3+ with the aid of tris(hydroxymethyl) aminomethane (Tris), forming Ce3+–ATP–Tris CPNs. In the presence of ALP, ATP will be catalytically converted into adenosine and inorganic orthophosphate, however neither of them can sensitize Ce3+ in alkaline media. As a result, the obtained CPNs are disassociated, inducing the quenching of the fluorescence. On this basis, a straightforward fluorescence assay for ALP activity is rationally developed. The fluorescence quenching efficiency shows a linear relationship for ALP within the activity range from 0.1 to 10 mU mL−1 with a detection limit of 0.09 mU mL−1 under the optimal experimental conditions. Moreover, this facile yet effective fluorescence method featured simplicity, cost-effectiveness, high sensitivity and high selectivity and can be successfully utilized for the quantitative detection of ALP in human serum samples.
研究领域:
生物化学
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医学诊断
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纳米技术
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DOI
PubMed
高影响因子
Small
·
2025
·
IF 12.1
Bingzhao Wu,Lianning Li,Hu Huang,Zhiyu Feng,Xinlong Zhang,Lei Zhang,Chunyu Zhao
Bottom-up construction of artificial cells is pivotal for investigating the functional and structural evolution of protocell systems. Here, a streamlined methodology is reported for constructing a protocell model that exhibits pH-adaptive responsiveness and functions as an efficient microreactor for cascade biochemical reactions. These protocells are engineered through liquid–liquid phase separation (LLPS) of poly(diallyldimethylammonium chloride) (PDDA) and adenosine triphosphate (ATP), yielding coacervate microdroplets that serve as nucleation sites for spontaneous self-assembly of phospholipid semipermeable membranes. By tuning the pH environment of the membranized protocells, the hydrolytic property of ATP under acidic conditions modulates the surface charge density of coacervates, triggering elevated surface potential and altered interfacial tension, which ultimately induce secondary phase separation inside the protocells to yield dynamically reconfigurable microcompartments. Notably, introduction of small-molecule substrates triggers enzymatic reactions within the molecularly crowded lumen of the protocells, which in turn drives pH-mediated micro-compartmentalization, enabling the coupling of biocatalysis with structural dynamics. This protocell model, characterized by programmable dynamic micro-compartmentalization, offers distinct advantages for applications in cellular mimicry, synthetic protobiology, and the development of artificial biomolecular microreactors.
研究领域:
合成生物学
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生物物理学
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分子自组装
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原始细胞模拟
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酶催化级联反应
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DOI
PubMed
高影响因子
ORGANIC & BIOMOLECULAR CHEMISTRY
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2016
·Vol. 14
·期 9
·2684-2690
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IF 3.559
Kui Wang,Jian-Hua Cui,Si-Yang Xing,Hong-Xi Dou
We have successfully implemented the supramolecular tandem assay principle for the real-time, continuous, direct, and label-free monitoring of alkaline phosphatase activity through a fluorescence “switch-off” assay based on a novel calixpyridinium/dye reporter pair. Because several diseases can be preliminarily diagnosed in light of an abnormal level of alkaline phosphatase in serum, the application of tandem assays to selectively monitor alkaline phosphatase activity has feasible implications in disease diagnosis.
研究领域:
生物化学
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分析化学
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诊断学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2023
·
IF 15.1
Lunjie Huang,Hongbin Pu,Da-Wen Sun
Nanozyme therapy holds promise for overcoming daunting antimicrobial resistance, but skills to target the microbial microenvironment are lacking. Herein, an isoreticular principle was introduced in nanozyme discovery to directedly evolve a domino biomimetic reaction specific to drug-resistant bacterial biofilm. The isoreticular Ce-UiO-66-X (X = BDC, BDC-CH 3 , BDC-OH, BDC-NH 2 , BDC-NO 2 , ADC, Fum) MOFs exhibited linker-dependent mimicry of hydrolytic apyrase and redox oxidase, and the phosphorous reactants (ATP/ADP/AMP/Pi) produced by the ATP hydrolysis could spontaneously activate redox reaction in Ce-UiO-66-X. Evidenced by the representative Ce-BDC-X (X = –NO 2 , -H, and –NH 2 ) nanozymes, the unique domino reaction of Ce-UiO-66-X could simultaneously deplete ATP molecules and trigger enhanced generation of superoxide radicals at the specific time window in the initial bacterial adhesion stage of methicillin-resistant Staphylococcus aureus (MRSA) biofilm formation. Due to the synergistic nanocatalytic mechanism, possible nanoscale penetration effect, and good biocompatibility, Ce-UiO-66-NO 2 could effectively target and destroy the metabolic microenvironment of MRSA biofilm in vitro and in vivo, reducing the MRSA biofilm viability to as low as 3.64%. This work demonstrates a new prodrug-like enzymodynamic antibacterial therapeutics based on cooperative multienzyme reactivity.
研究领域:
纳米技术
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生物化学
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微生物学
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材料科学
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治疗学
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产品关联
DOI
高影响因子
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