JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
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IF 6.1
Zhiqi Zhang,Hanchuan Wang,Zhulingzhi Kuang,Hui Liang,Yan Ju,Dali Meng
Abrus mollis Hance is a characteristic medicinal herb which is used in Guangdong and Guangxi provinces of China for making soup, medicinal meals, and herbal tea to treat dampheat jaundice and rib discomfort. Current phytochemical study on A. mollis led to the isolation of four new flavones, mollisone A-D (1–4), and thirty two known compounds (5–36). Their structures were characterized by an extensive analysis of spectroscopic data including IR, UV, HR-ESI-MS, and 1D and 2D NMR, as well as electronic circular dichroism calculation. In addition, in order to initially understand their biological activities for traditional applications, in vitro antioxidant and hepatoprotective tests were carried out, whose results illustrated that 25 compounds had significant free radical scavenging ability, and compounds 13 and 16 exhibited protective activities on D-GalN-induced LO2 cell damage than the positive control. Moreover, network pharmacological analysis revealed that the hepatoprotective activity of A. mollis involved multitargets and multipathways such as PI3K/Akt, MAPK, and JAK-STAT pathways and various biological processes such as positive regulation of phosphorylation and regulation of kinase activity. These results suggested that this species could serve as a potential hepatoprotective agent for functional food or medicinal use.
研究领域:
中药学
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药理学
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植物化学
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DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
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2023
·Vol. 20
·期 6
·e202300204
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IF 2.9
Zhike Liang,Zhixia Chen,Xianmei Xue,Cuimin He,Xujiang Yuan
Abrus mollis (MJGC) has been used as a substitute herb for Abrus cantoniensis (JGC) in China. However, an in-depth comparison on their key metabolites and the mechanism of anti-inflammation between these two is not available. In this report, high pressure liquid chromatography equipped with mass spectrometry was applied to capture their flavonoid profiles; transcriptomics was adopted to analyze their anti-inflammatory mechanisms. The results showed that the main flavonoids in MJGC were vicenin-2, schaftoside and isoschaftoside, while those in JGC were vicenin-1 isomer and schaftoside isomer. The anti-inflammatory activity of JGC was slightly stronger than that of MJGC. The number of differential expression genes regulated by JGC was significantly higher than MJGC. JGC regulated 151 (42 up and 109 down) of inflammation related genes, while MJGC regulated 58 (8 up and 50 down) of inflammation related genes. The results of this study provided scientific evidence and guidance for the substitution of MJGC and JGC.
研究领域:
药用植物学
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药学
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转录组学
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植物化学
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PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
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2022
·Vol. 46
·期 2
·2200311
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IF 3.614
Zhien Tan,Shimin Chen,Mengli Zhang,Xiaosheng Qu,Taiping Li,Aihua Zhang,Yanmei He,Min Ou,Lihuo Long,Lu Chen,Fangfang Wu
Abrus mollis Hance is a traditional Chinese medicine that is widely used to treat acute and chronic hepatitis, steatosis, and fibrosis. Its therapeutic qualities of it have long been acknowledged, although the active ingredients responsible for its efficacy and the mechanisms of its action are unknown. In this study, the chemical constituents absorbed into the blood from Abrus mollis Hance were assessed by using liquid chromatography-quadrupole-time-of-flight mass spectrometry and the data was analyzed with the UNIFI screening platform. The results obtained were compared to existing chromatographic-mass spectrometry information, including retention times and molecular weights as well as known reference compounds. 41 chemical constituents were found in Abrus mollis Hance, and these included 16 flavonoids, 13 triterpenoids, five organic acids, and two alkaloids. Experimentally it was found that Abrus mollis Hance had a therapeutic benefit when treating α-naphthalene isothiocyanate-induced acute liver injury in rats. In addition, 11 blood prototypical constituents, including six flavonoids, three triterpenoids, and two alkaloids, were found in serum samples following intragastric administration of Abrus mollis Hance extracts to rats. This novel study can be used for the quality control and pharmacodynamic assessment of Abrus mollis Hance in order to assess its efficacy in the therapeutic treatment of patients.
研究领域:
中药学
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分析化学
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药理学
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PubMed
高影响因子
PLANTA MEDICA
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2021
·Vol. 87
·期 07
·570-580
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IF 3.356
Wei Shen,Xiaolong Hu,Yajun Niu,Yimeng Lu,Baolin Wang,Hao Wang
Abrus mollis is commonly used as a traditional Chinese medicine for the treatment of liver diseases due to its hepatoprotection and anti-inflammation, but the absorption properties of its main bioactive ingredients remain unclear. Our previous studies verified that the flavonoid C-glycosides, including vicenin-2 (1), isoschaftoside (2), and schaftoside (3), were the major active components in A. mollis for hepatic protection against nonalcoholic fatty liver disease, hepatitis, and hepatic fibrosis. This study investigated the bioaccessibility and transport mechanisms of total flavonoid C-glycoside, as well as vicenin-2 (1), isoschaftoside (2), and schaftoside (3), in A. mollis by simulated digestion and use of the Caco-2 cell model. Moreover, this study attempted to verify their absorption properties by in situ gastrointestinal perfusion in rats. Total flavonoid C-glycoside and 1, 2, and 3 exhibited similar bioaccessibility of 84.58%, 85.13%, 83.05%, and 81.65% respectively after simulated digestion. The transport of total flavonoid C-glycoside in the Caco-2 cell model increased with the concentration, and the transport showed saturation characteristics with the time and concentration of total flavonoid C-glycoside to a certain degree. The Papp values of total flavonoid C-glycoside and the 3 flavonoid C-glycosides were significantly improved by verapamil, probenecid, and EDTA-Na2. Their absorption properties in the gastrointestinal tract were consistent with that found in Caco-2 cells, and superior absorption rates were observed in the duodenum and jejunum. The absorption pattern of total flavonoid C-glycoside may involve multiple transport pathways, including active transport, passive diffusion, and the paracellular pathway. TFC was actively pumped out by P-glycoprotein and multidrug resistance-associated protein. These results revealed that the bioaccessibility and intestinal absorption characteristic of total flavonoid C-glycoside were consistent with the 3 major flavonoids.
研究领域:
药理学
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中药学
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消化与吸收机制
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高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2025
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IF 5.4
Mingjuan Huang,Xue Wang,Yixiang Lu,Qiaowen Zhou,Jiemei Liang,Quanxin Li,Liangbo Li,Lanlan Fan
Ethnopharmacological relevance Abrus mollis Hance (AM), a traditional medicinal used for hepatoprotection in the south of China, has demonstrated potential in ameliorating lipid metabolism disorders (LMD). However, the underlying mechanisms remain elusive. Aim of the study By integrating metabolomics and network pharmacology, the study explores how AM regulates lipid metabolism in mice induced by a high-fat diet (HFD). Materials and methods An HFD-induced dyslipidemia mice model was established to evaluate the therapeutic effectiveness of AM. Untargeted metabolomics identified hepatic endogenous metabolites and associated pathways. Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) characterized bioactive compounds and blood-absorbed components in AM. Network pharmacology analysis of blood-absorbed components, combined with molecular docking, predicted potential active constituents targeting lipid metabolism disorders, identified critical targets, and mapped metabolic pathways. Core regulatory mechanisms were elucidated through integrative analysis of network pharmacology predictions and metabolomics data. Transcriptional levels and protein expression of related genes were analyzed in murine hepatic tissues using real-time quantitative PCR (RT-qPCR) and Western blotting. Results Animal experiments have confirmed the efficacy of AM in alleviating lipid abnormalities induced by HFD. Twenty-one hepatic endogenous metabolites were identified, with major pathways encompassing arachidonic acid metabolism, linoleic acid metabolism, among others. UPLC-Q-TOF/MS analysis characterized 50 bioactive compounds in AM, 24 of which were detected in plasma. Network pharmacology prioritized schaftoside, hyperoside, protocatechuic acid, and vitexin as core components targeting TP53, SRC, ESR1, and PIK3CA, while molecular docking validated their strong binding affinities. KEGG analyses ultimately indicated that the PI3K-Akt signaling pathway is the key pathway. Integrated analyses of metabolomics and network pharmacology data further confirmed that the PI3K/Akt signaling pathway is the core regulatory pathway. RT-qPCR and WB further evidenced AM-mediated downregulation of PI3K/Akt pathways-associated transcription factors and proteins. Conclusion This study elucidated the multi-target mechanisms of AM via the PI3K/Akt signaling pathway and highlighted its therapeutic potential in modulating lipid metabolism disorders.
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高影响因子
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