CURRENT MICROBIOLOGY
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2016
·Vol. 74
·期 2
·193-202
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IF 1.519
Xia Zhen-Yuan,Zhang Long,Zhao Yan,Yan Xin,Li Shun-Peng,Gu Tao,Jiang Jian-Dong
In this study, a bacterial strain of Achromobacter sp. LZ35, which was capable of utilizing 2,4-dichlorophenoxyacetic acid (2,4-D) and 2-methyl-4-chlorophenoxy acetic acid (MCPA) as the sole sources of carbon and energy for growth, was isolated from the soil in a disused pesticide factory in Suzhou, China. The optimal 2,4-D degradation by strain LZ35 occurred at 30 °C and pH 8.0 when the initial 2,4-D concentration was 200 mg L −1 . Strain LZ35 harbored the conserved 2,4-D/alpha-ketoglutarate dioxygenase (96%) and 2,4-dichlorophenol hydroxylase (99%), and catabolized 2,4-D via the intermediate 2,4-dichlorophenol. The inoculation of 7.8 × 10 6 CFU g −1 soil of strain LZ35 cells to 2,4-D-contaminated soil could efficiently remove over 75 and 90% of 100 and 50 mg L −1 2,4-D in 12 days and significantly released the phytotoxicity of maize caused by the 2,4-D residue. This is the first report of an Achromobacter sp. strain that was capable of mineralizing both 2,4-D and MCPA. This study provides us a promising candidate for its application in the bioremediation of 2,4-D- or MCPA-contaminated sites.
研究领域:
环境微生物学
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生物修复
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环境科学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2020
·
IF 9.038
Dong Hui Liang,Yongyou Hu
Little information is available regarding the kinetics, products, and pathways of simultaneous SMX degradation and Cd(II) immobilization from wastewater. In this study, a novel bacterium ( Achromobacter sp. L3) with SMX degradation and Cd(II) immobilization capabilities was isolated. The boundary conditions of SMX degradation were as follows: initial pH 6–8, temperature 25–30 °C, and SMX concentration 10–40 mg/L −1 . The boundary conditions of Cd(II) immobilization were as follows: initial pH 7–9, temperature 25–35 °C, and SMX concentration 10–30 mg/L −1 . The maximum SMX degradation and Cd(II) removal were 91.98% and 100%, respectively. The SMX degradation and Cd(II) immobilization data fitted well with the pseudo-first-order kinetic model, indicating that the two pollutants conform to the same degradation rule. Moreover, the microbial degradation , sediment adsorption, and intermediates identified in the experiments were used to explore the mechanisms of SMX and Cd(II) removal. These results indicate that microbial removal and sediment adsorption play equally important roles in Cd(II) immobilization; however, microbial degradation plays a decisive role in SMX degradation. Furthermore, the relationship between aerobic denitrification, SMX degradation, and Cd(II) immobilization was proposed. These results may provide valuable insights for treatment of wastewater polluted by antibiotics and heavy metals.
研究领域:
环境微生物学
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废水工程
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污染控制
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PubMed
高影响因子
PLoS One
·
2018
·Vol. 13
·期 10
·e0204324
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IF 2.766
Fangfang Li,Yidan Liu,Diwei Wang,Chaosheng Zhang,Zhihui Yang,Siqi Lu,Yangyang Wang
A halotolerant bacterial consortium capable of degrading di-(2-ethylhexyl) phthalate (DEHP) was enriched from activated sludge. Community analysis revealed that LF contained seven families and seven genera of bacteria. The predominant species was Gordonia sp. (54.93%), Rhodococcus . sp. (9.92%) and Achromobacter sp. (8.47%). The consortium could degrade 93.84% of 1000 mg/l DEHP after 48 h incubation. The optimal temperature and pH for LF to degrade DEHP were 30 °C and 6.0, respectively. LF degraded more than 91% of DEHP with salt concentrations ranging from 0–3%. The inoculum size had great effects on DEHP degradation (incubation time < 24h). LF could degrade high concentrations of DEHP (from 100 to 2000 mg/l) with the degradation ratio above 92% after 72 h incubation. Kinetics analysis revealed that the degradation of DEHP by LF was best fitted by the first-order kinetics when the initial concentration ranged from 100 to 2000 mg/l. The main intermediates (2-ethylhexyl pentyl phthalate, butyl (2-ethylhexyl) phthalate (BEHP), mono-ethylhexyl phthalate (MEHP), mono-hexyl phthalate (MHP), mono-butyl phthalate (MBP)) in DEHP degradation process were identified using gas chromatography–mass spectrometry (GC-MS), and a new complex biochemical pathway was proposed. Furthermore, LF could also degrade dimethyl phthalate (DMP), diethyl phthalate (DEP), di- n -butyl phthalate (DBP), di- n -octyl phthalate (DOP) and phthalic acid (PA).
研究领域:
环境科学
·
微生物学
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DOI
高影响因子
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
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2024
·
IF 2.3
Mengyao Gui
Humic acid (HA), a common natural organic matter , could affect conventional anoxic denitrification. Aim of this study was to investigate effect of HA on the process of aerobic denitrification in Achromobacter sp. GAD-3, an aerobic denitrifying strain. The findings demonstrated that an increase in HA concentrations (≥5 mg L −1 ) promoted the aerobic denitrification process (excluding N 2 O reduction), manifesting as higher rates of nitrate removal (6.67–11.1 mg L −1 h −1 ) and lower levels of nitrite accumulation (30.2–20.7 mg L −1 ). This was attributed to the increased electron transfer activities and denitrifying reductase activities (including NAR, NIR and NOR) facilitated by HA. Accordingly, the expression of denitrification genes such as napA , cnorB , and nirS was enhanced by HA. Nonetheless, the nosZ gene and N 2 OR activity underwent suppression by HA, which was accountable for N 2 O emission. It is crucial to understand the HA mechanism towards aerobic denitrifiers for wastewater treatment plants to enhance nitrogen removal.
研究领域:
环境生物学
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废水处理
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产品关联
DOI
PubMed
高影响因子
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