CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Zezhou Lin,Tianyu Li,Qian Yu,Haiyun Chen,Di Zhou,Ning Li,Chunyan Yan
Achyranthes bidentata is a species of flowering plant that is mainly distributed in China. The A. bidentata rhizome is a famous traditional Chinese medicine that has been widely used to treat lumbago, arthritis, and bone hyperplasia. In this work, A. bidentata rhizome was isolated and purified to obtain a pectic polysaccharide (ABPB-4). Chemical and spectral analyses showed that ABPB-4 had a main chain of →4)- α - d -Gal p A-(1→ and →2,4)- α - l -Rha p -(1→, and the branch chains included →4)- β - d -Gal p -(1→, →6)- β - d -Gal p -(1→, →3,6)- β - d -Gal p -(1→, →5)- α - l -Ara f -(1→ and →3,5)- α - l -Ara f -(1→, and it was terminated with α - l -Ara f -(1→ and β - d -Gal p -(1→. At concentrations of 0.01, 0.02, and 0.04 μmol/L, ABPB-4 significantly promotes the proliferation, differentiation, and mineralization of MC3T3-E1 cells in vitro , and it appreciably enhances the mRNA expression levels of osteogenic-related genes in these cells. Overall, the results reported herein indicate that ABPB-4 has outstanding osteogenic activity, and that it may be used as an anti-osteoporosis agent in the future.
研究领域:
中药学
·
分子生物学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FITOTERAPIA
·
2022
·
IF 3.204
Meixi He,Yingang Feng,Yi Wang,Mengchun Cheng,Xiaozhe Zhang,Lihua Zhang
Cysteine-rich peptides (CRPs) are stable molecules that contain multiple disulphide bonds . Various CRPs are found in plants and animals, representing potential compounds for drug development with diverse activities. Modification of CRPs, such as glycation, has attracted increased attention due to its special structural and functional properties. Hence, this study explored a CRP isolated from the Chinese herb Achyranthes bidentata Blume, which contains a glycation modification. Herein, a reverse phase high-performance liquid chromatography system with mobile phases was used to extract and purify the peptide. The eluted peptide was detected using high resolution mass spectrometry and structurally identified using high resolution mass spectrometry and nuclear magnetic resonance. The effect of the peptide on the viability of N -methyl-D-aspartic acid (NMDA)-induced HT22 cells was determined using a cell assay. Here, a new cysteine-rich glycation peptide, termed glycation-bidentatide (Gly-BTP), with three pairs of disulphide bonds and a glycation modification at the N-terminus linked to cysteine, was discovered. Cell bioactivity assay results suggested that Gly-BTP might be a potential therapeutic and provide a neuroprotective effect in NMDA-induced HT22 murine hippocampal neuronal cells. The discovery of Gly-BTP will promote the understanding of the role of CRPs in neuroprotection .
研究领域:
药理学
·
生物化学
·
神经科学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2019
·
IF 3.414
Yi Tao,Surun Huang,Jizhong Yan,Weidong Li,Baochang Cai
Ethnopharmacological relevance Achyranthes bidentata Blume (AB) is a well-known traditional Chinese medicine for treating osteoporosis and bone fracture. In the current, researches on pharmacological mechanism of AB mostly focused on molecular pathways, knowledge about its metabolic signatures is largely unclear. This study aims to develop an integrative metabolomics and metallomic approach for deciphering the biochemical basis of anti-osteoporosis effects of raw and salt-processed AB. Method Gas chromatography–mass spectrometry (GC–MS) and inductively coupled plasma mass spectrometry (ICP-MS) were combined for metabolomic and metallomic profiling of rats serum, liver and kidney derived from the sham group, model group, E2, raw and salt-processed AB treated groups. Meanwhile, micro-CT and biomechanical analysis were carried out to ensure the success of the osteoporosis model and to validate the anti-osteoporosis effect of raw and salt-processed AB. Partial least squares discriminant analysis (PLS-DA) was employed to screen potential biomarkers and the MetaboAnalyst and KEGG PATHWAY Database were used to investigate the metabolic pathway. Results Raw and salt-processed AB protected the rats against osteoporosis, as evidenced by the restoration of the alkaline phosphatase activity, osteocalcin concentration, urine calcium/creatinine ratio and urine phosphorus/creatinine ratio. The combination of PCA and PLS-DA revealed deviations in ninety-four differential biomarkers between raw AB treated group and model group. The identified biomarkers were primarily engaged in the metabolic pathways including galactose metabolism, urea cycle, arginine and proline metabolism, alanine metabolism, lactose degradation, ammonia recycling and glycine and serine metabolism. The levels of these biomarkers showed significant alterations and a tendency to be restored to normal values in raw and salt-processed AB treated osteoporosis rats. Of note, the levels of trace elements, such as Zn, Se, Mn, Cu and Fe, were elevated after raw and salt-processed AB treatment. Finally, a correlation network diagram was constructed to show the biomarkers perturbed by raw and salt-processed AB. Conclusion Our findings indicate that raw and salt-processed AB has positive effects on osteoporosis rats. Meanwhile, metabolomic and metallomic method coupled with metabolites enrichment analysis and pattern recognition serves as a useful tool for revealing the action mechanism of traditional Chinese medicine.
研究领域:
药理学
·
代谢组学
·
中医药
·
骨质疏松研究
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Chunyan Yan,Shaojie Zhang,Changsheng Wang,Qian Zhang
Achyranthes bidentata is used as a medicinal herb prescribed for the treatment of osteoporosis in China. The anti-osteoporosis activity of the crude polysaccharide from A. bidentata , AB50, was evaluated by ovariectomized rats. The results indicated that AB50 has prominent osteoprotective effects, which was comparable to positive control. To further explore which ingredients are responsible for these effects, a novel fructan, ABW50-1 (1260 Da), was purified for the first time and characterized by chemical methods and nuclear magnetic resonance. The results revealed that ABW50-1 was composed of →2)- β -D-Fru f -(1→, →2)- β -D-Fru f -(1, 6→ and →2)- β -D-Fru f -(6→, terminated with Glu and Fru residues. Morphological analyses indicated that ABW50-1 was fragmented and contained a branched structure. Furthermore, ABW50-1 significantly increased the relative fluorescence intensity of bone mass, without side effects, in a zebrafish model of glucocorticoid-induced osteoporosis (GIOP). Thus, ABW50-1 shows potential for developing new anti-osteoporosis agents for osteoporosis therapy.
研究领域:
药理学
·
药物化学
·
骨骼生物学
·
中医
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2017
·
IF 3.181
Changsheng Wang,Dawei Zhang,Mengliu Zhang,Yukun Jiao,Keming Jiang,Chunyan Yan
Achyranthes bidentata Blume is one of the most frequently used medicinal herbs prescribed for treatment of osteoporosis in China. This study describes the crude saccharide (AB90) from A. bidentata and reports the osteoprotective effects of AB90 in ovariectomized (OVX) rats. The results indicated that AB90 significantly increased the bone mineral content (BMC) and bone mineral density (BMD) and improved the biomechanical properties in OVX rats. To further explore the fraction(s) responsible for above activity, a novel oligosaccharide (ABW90-1) was isolated continuously by DEAE-Cellulose 52 and Sephacryl S-100 filtration columns and characterized by infrared spectroscopy, monosaccharide analysis, methylation and NMR analysis. It was composed of (2 → 6)-linked- β - d -fructofuranosyl (Fru f ), (2 → 1)-linked- β - d -Fru f residues and was terminated with Glc and Fru residues. The advanced structure and conformation of novel polysaccharide ABW90-1 were investigated by circular dichroism (CD) and scanning electron microscopy (SEM) data, and the results indicated that ABW90-1 was composed of randomly distributed sheet-like appearances instead of a triple-helix structure. In addition, in vitro activity of ABW90-1 was evaluated by using primary osteoblasts, and the data suggested that ABW90-1 exhibited favorable effects on the proliferation and differentiation of primary osteoblasts.
研究领域:
药理学
·
生物化学
·
分子生物学
·
药学
✓ 已验证
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
·
IF 3.4
HuaYang Si,Yimeng Chen,Jie Yang,Xiaodong Wen
Although the roots of Achyranthes bidentata (AB), Cyathula officinalis (CO) and Achyranthes aspera (AA) are different drugs, they are always confused in clinical practice due to their similar chemical components and functions. As polysaccharides are abundant in these drugs, a systematic comparison of polysaccharides from AB, CO and AA is not only necessary to understand their similar but not identical functions, but also helpful for the quality control of them. In this study, polysaccharides from 22 batches of AB, CO and AA were compared with monosaccharide composition , molecular weight distribution and saccharide mapping. Polysaccharides of AB, CO and AA had similar monosaccharide compositions but their relative contents of fructose, glucuronic acid , galacturonic acid and glucose were significant different, and could be used as key markers to distinguish them. Results from molecular weight distribution and saccharide mapping showed polysaccharides from AB, CO and AA were mainly composed of fructans with β-2,1 and β-2, 6- D -fructosidic linkages, but their degree of polymerization were different. Meanwhile, pectins were also contained in these three drugs. AB is partial to immunomodulation while CO is partial to removing blood stasis . Fructans and pectins are the similar bioactive substance basis of AB, CO and AA whereas their structural difference might be contributed to the efficacy differentia of these three drugs. This study provides a better understanding on the profiles of polysaccharides from AB, CO and AA, further guiding their clinical usage and facilitating their quality control.
研究领域:
生药学
·
药物化学
·
中医药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today Advances
·
2026
·
IF 8
Guanxiong Liu,Yihan Li,Hui Wang,Hongchen Zhu,Junli Fan,Chunyu Wan,Minghao Wang,Yan Chen,Changqian Zhou,Qun Wang,Hongxia Zhao,Xin Li,Lijuan Gui,Lei Zhang,Shuang Jiang,Yingping Li,Yuqi Wang,Zonglin Liu,Peizhi Zhu,Yu Zhu
Biomass-derived carbon dots (CDs) are increasingly recognized as biocompatible, multifunctional nanomaterials with strong potential in agricultural applications. Herein, we report a high-yield hydrothermal synthesis of antibiotic-derived CDs with tailored functionalities for enhancing the growth of Achyranthes bidentata , one widely used Traditional Chinese Medicine plants. By introducing acrylamide as a co-carbon source and potassium persulfate as a polymerization initiator, the synthetic yield was dramatically improved (up to 90.5%), and the resulting CDs exhibited a polyacrylamide-like structure that significantly enhanced soil water retention. Furthermore, CDs derived from tetracycline and quinolone antibiotics demonstrated reactive oxygen species scavenging capabilities, mitigating oxidative stress. Application of these CDs promoted root, stem, and leaf growth. Additionally, the antibiotic-derived CDs exhibited significant bone-binding affinity due to Ca 2+ chelation, highlighting their potential for bone-defect localization and tracking. This work provides a scalable and multifunctional nanomaterial strategy for sustainable agriculture, medicinal plant cultivation, and potential biomedical applications.
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Huayang Si,Yimeng Chen,Dejun Hu,Sainan Yao,Jie Yang,Xiaodong Wen
Diabetic kidney disease (DKD) is the main cause of end-stage renal disease, and few therapeutic options are available. The root of Achyranthis bidentatae (AB) is commonly used for DKD treatment in Traditional Chinese medicine. However, its mechanisms are still unclear. Here, a graminan type fructan ABPW1 with molecular weight of 3998 Da was purified from AB. It was composed of β-1,2-linked Fru f , β-2,6-linked-Fru f and β-1,2,6-linked-Fru f backbone, and terminated with T-Glc p and 2-Fru f residues. ABPW1 protected against kidney injuries and intestinal barrier disruption in Streptozotocin (STZ)/High fat diet (HFD) mice. It could modulate gut microbiota composition, evidenced by a rise in the abundance of Bacteroide and decreases of Rikenella , Alistipes , Laedolimicola and Faecalibaculum . ABPW1 intervention promoted short chain fatty acids (SCFAs) production in STZ/HFD mice, especially propionate and isobutyric acid. Antibiotic treatment further demonstrated the key role of gut microbiota in the renal protective action of ABPW1. In addition, in vitro simulated digestion and fermentation together with in vivo fluorescent labeling studies demonstrated ABPW1 was indigestible in upper digestive tract but could reach the colon and be degraded into SCFAs by gut microbiota there. Overall, these data suggested ABPW1 has the potential application on DKD prevention.
研究领域:
药理学
·
微生物学
·
肾病学
·
中医
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nutrients
·
2025
·Vol. 17
·期 20
·3249
·
IF 5
Ting Xia,Zhenjie Liu,Wenya Ding,Liting Deng,Xinyang Ning,Jianfang Feng
Background/Objectives:Achyranthes bidentata(AB), recognized as a food and traditional Chinese medicine, exhibits notable biological activity. Our previous study showed the hypoglycemic effect ofAchyrantha bidentata polysaccharide(ABP). The properties and digestion process of polysaccharides affect their pharmacological activities. The digestion characteristics of ABP are unclear. In this study, we aimed to explore the characteristics of ABP’s simulated digestion and its prebiotic properties and hypoglycemic effects.Methods: We used simulated digestion methods to investigate the alterations in ABP levels in the process of digestion and fermentation. Animal experiments were used to compare the effects of the prebiotic properties of ABP on normal control (NC) and type 2 diabetes mellitus (T2DM) mice. Then, in order to further verify the hypoglycemic effect of ABP after fermentation (ABPF), α-glucosidase activity and glucose uptake in Caco-2 cells were examined.Results: The results showed that ABP was anti-digestive and mainly degraded by the intestinal flora. Moreover, ABP showed a stronger promoting advantage against beneficial bacteria and inhibited harmful bacteria in the T2DM mice. Compared with NC mice, after ABP treatment, T2DM mice showed a higher increase in levels of short-chain fatty acids (SCFAs). Additionally, the glucose uptake and α-glucosidase activity of Caco-2 cells were significantly decreased after treatment with ABPF.Conclusions: These results underscore the potential of ABP as a prebiotic candidate for gut health promotion and T2DM alleviation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）