Frontiers in Cellular and Infection Microbiology
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2021
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IF 5.293
Zhang Wei,Li Zhixing,Yang Haitao,Wang Guanglu,Liu Gang,Wang Yu,Bello Babatunde Kazeem,Zhao Panpan,Liang Wei,Dong Jingquan
Aeromonas sobria, a common conditional pathogenic bacteria, is widely distributed in the environment and causes gastroenteritis in humans or septicemia in fish. Of all Aeromonas species, A. sobria is the most frequently isolated from human infections especially in immunocompromised subjects. Innate immunity is the first protection system of organism to resist non-specific pathogens invasion; however, the immune response process of hosts against A. sobria infection re\mains unexplored. The present study established an A. sobria infection model using primary mouse peritoneal macrophages (PMφs). The adherence and cytotoxicity of A. sobria on PMφs were determined by May-Grünwald Giemsa staining and LDH release measurement. Pro-inflammatory cytokine expression levels were measured using qPCR, western blotting, and ELISA methods. We also investigated the levels of ASC oligomerization and determined the roles of active caspase-1 in IL-1β secretion through inhibition assays and explored the activated pattern recognition receptors through immunofluorescence. We further elucidated the roles of activated inflammasome in regulating the host’s inflammatory response through inhibition combined with ELISA assays. Our results showed that A. sobria induced lytic cell death and LDH release, whereas it had no adhesive properties on PMφs. A. sobria triggered various proinflammatory cytokine transcription level upregulation, and IL-1β occupied the highest levels. The pro-IL-1β protein expression levels increased in a dose-dependent manner with MOI ranging from 1 to 100. This process was regulated by ASC-dependent inflammasome, which cleavage pro-IL-1β into active IL-1β p17 with activated caspase-1 p20. Meanwhile, the expression levels of NLRP3 receptor significantly increased, location analysis revealed puncta-like surrounding nuclear, and inhibition of NLRP3 inflammasome downregulated caspase-1 activation and IL-1β secretion. Blocking of NLRP3 inflammasome activation through K+ efflux and cathepsin B or caspase approaches downregulated A. sobria–induced proinflammatory cytokine production. Overall, these data indicated that A. sobria induced proinflammatory cytokine production in PMφs through activating NLRP3 inflammasome signaling pathways.
研究领域:
免疫学
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微生物学
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细胞生物学
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传染病学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD QUALITY
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2023
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IF 3.3
Jing-Xin Ye,Yun-Fang Qian,Wei-Qing Lan,Jing Xie
Biofilms composed of microbes and extracellular polymeric substances (EPSs) pose a significant risk to human health and lead to economic loss in the food industry. In this study, the antimicrobial and antibiofilm properties of kojic acid (KA) against Aeromonas sobria (A. sobria) and Staphylococcus saprophyticus (S. saprophyticus) were investigated by determining the leakage of DNA and protein, cell morphology, biofilm formation, the metabolic activity of biofilms, excretion of EPS, and biofilm architecture. The results indicated that the values of minimum inhibitory concentration (MIC) of A. sobria and S. saprophyticus after KA treatment were 0.4 mg/mL and 1.6 mg/mL, respectively. 1 × MIC KA showed unignorable antimicrobial activity against the two bacteria, leading to alterations in the bacterial physicochemical characteristics and cell death. Sub-MICs of KA can inhibit biofilm formation and decrease the metabolic activity and excretion of EPS, and these inhibition effects were in a dose-dependent manner. These results were further confirmed by the visual images obtained from scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Moreover, S. saprophyticus is more susceptible to KA in inhibiting biofilm formation, and for A. sobria, changes in the cell structure and the permeability of the cell membrane were more obvious. This research highlighted the antibacterial and antibiofilm activity of KA against A. sobria and S. saprophyticus.
研究领域:
微生物学
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生物技术
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食品科学
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DOI
高影响因子
Food Packaging and Shelf Life
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2025
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IF 10.6
Yanqing Li,Xuchen Li,Shaomei Li,Jiao Li,Xiang Zou,Hongwei Zhan,Guanyuan Qu,Lixiu Zhang,Yongjun Sun,Wenming Ju,Mengqi Ye,Pengdong Sun,Jingyuan Li,Yang Deng,Ting Ding
Aeromonas sobria , recognized as a psychrotrophic specific spoilage organism in refrigerated aquatic products, facilitates its proliferation by metabolizing proteins, lipids, and carbohydrates, thereby generating spoilage-associated metabolites and compromising product quality. Quorum sensing (QS), a bacterial cell-to-cell communication mechanism, orchestrates the secretion of virulence and spoilage factors in A. sobria via the LuxI/R QS system. Targeting this regulatory pathway presents a novel strategy to mitigate spoilage and extend the shelf life of aquatic products. Previous studies have demonstrated that zingerone (ZRN) exhibits quorum-quenching activity at sub-inhibitory concentrations; however, its practical application is constrained by limited solubility and bioavailability. To overcome these challenges, ZRN was encapsulated within halloysite nanotubes (HNTs) and incorporated into a self-assembled composite film composed of chitosan (CS) and sodium carboxymethyl cellulose (CMC-Na). The fabricated film was characterized for its structural, thermal, and mechanical properties and subsequently evaluated for its preservation efficacy on refrigerated perch fillets. The composite film exhibited a homogeneous surface morphology with uniformly dispersed HNTs, alongside adequate thermal stability, tensile strength, and physicochemical attributes suitable for refrigerated aquatic product preservation. Notably, the film suppressed spoilage indicators, including total volatile basic nitrogen (TVB-N) accumulation, lipid peroxidation, and bacterial spoilage potential, without inhibiting A. sobria growth. Furthermore, it mitigated muscle tissue degradation and biogenic amine formation in perch fillets during refrigerated storage. Molecular docking simulations revealed that ZRN competitively binds to the LuxR-type receptor at the same binding pocket as the endogenous autoinducer C 4 -HSL, thereby disrupting QS signaling. Collectively, this ZRN-loaded composite film represents a promising biodegradable packaging solution for enhancing the quality and shelf life of refrigerated aquatic foods.
研究领域:
食品微生物学
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食品保鲜
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纳米技术
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细菌通讯
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材料科学
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水产品加工
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）