ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Huan Tang,Sheng-Tao Yang,Yi-Fan Yang,Da-Ming Ke,Jia-Hui Liu,Xing Chen,Haifang Wang,Yuanfang Liu
As a novel fluorescent probe in the second near-infrared window, Ag2Se quantum dots (QDs) exhibit great prospect in in vivo imaging due to their maximal penetration depth and negligible background. However, the in vivo behavior and toxicity of Ag2Se QDs still largely remain unknown, which severely hinders their wide-ranging biomedical applications. Herein, we systematically studied the blood clearance, distribution, transformation, excretion, and toxicity of polyethylene glycol (PEG) coated Ag2Se QDs in mice after intravenous administration with a high dose of 8 μmol/kg body weight. QDs are quickly cleared from the blood with a circulation half-life of 0.4 h. QDs mainly accumulate in liver and spleen and are remarkably transformed into Ag and Se within 1 week. Ag is excreted from the body readily through both feces and urine, whereas Se is excreted hardly. The toxicological evaluations demonstrate that there is no overt acute toxicity of Ag2Se QDs to mice. Moreover, in regard to the in vivo stability problem of Ag2Se QDs, the biotransformation and its related metabolism are intensively discussed, and some promising coating means for Ag2Se QDs to avert transformation are proposed as well. Our work lays a solid foundation for safe applications of Ag2Se QDs in bioimaging in the future.
研究领域:
生物医学
·
药理学
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
Toxicology Research
·
2017
·Vol. 6
·期 5
·693-704
·
IF 1.969
Tang Huan,Yang Sheng-Tao,Ke Da-Ming,Yang Yi-Fan,Liu Jia-Hui,Chen Xing,Wang Haifang,Liu Yuanfang
Ag2Se quantum dots (QDs) are novel fluorescent probes in the second near-infrared window with great imaging quality and biocompatibility. Surface modification is an essential step to disperse Ag2Se QDs into biological fluids, and endow Ag2Se QDs with diverse surface chemistry. However, the effect of surface chemistry on the biological behaviors and chemical fates of Ag2Se QDs has not been studied, which hinders the design of suitable Ag2Se QDs for biomedical applications. Here, the distribution, degradation, excretion and toxicity of 2-aminoethanethiol and mercaptopropionic acid coated Ag2Se QDs (denoted as QDs-MEA and QDs-MPA, respectively) were systematically investigated in mice for a 28-day observation period after a single intravenous injection. Ag2Se QDs with different surface chemistries displayed similar trends in all observations, such as fast blood clearance, main uptake in the liver and spleen, severe biotransformation, Ag excretion through feces, and low toxicity. The major different behaviors observed were the partially pulmonary deposition, the faster transformation at the initial stage, the lower excretion percentage, and the more obvious damage to the liver by QDs-MEA compared to QDs-MPA. The surface chemistry of Ag2Se QDs regulated their biological behaviors and chemical fates in vivo, and surface chemistry should be fully regarded when designing Ag2Se QDs for biomedical applications from the biosafety perspective.
研究领域:
生物医学
·
纳米材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2016
·
IF 3.014
Dongchen Che,Di Ding,Hongzhi Wang,Qinghong Zhang,Yaogang Li
Efficient aqueous synthetic methods for near-infrared quantum dots as bioimaging agents are urgently required. In this work, a simple and fast synthesis of highly luminescent, near-infrared Ag 2 Se quantum dots (QDs) in aqueous media is reported. The method avoids high temperature, pressure and organic solvents to directly generate water-dispersible Ag 2 Se QDs. The photoluminescence emission of Ag 2 Se QDs ranges from 835 to 940 nm by different Ag:Se molar ratio. Using the ZnSe as a shell, the quantum yield reaches up to 42%. The Ag 2 Se ZnSe QDs with high quantum yield, near-infrared and low cytotoxic could be used as good cell labels, showing great potential applications in bio-imaging.
研究领域:
材料科学
·
生物医学工程
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2023
·
IF 5.9
Kai Wang,Ku-Han Deng,Yi-Shen Tian,Meng-Ying Sun,Zi-li Yu,Zhi-Quan Tian,Zhi-Ling Zhang
Fluorescence in the near infrared IIb window (NIR-IIb, 1500–1700 nm) shows higher signal-to-noise ratio, better space-time resolution, and deeper tissue penetration. However, materials with excellent fluorescence properties in the NIR-IIb region were rarely reported. Ag2Te quantum dots (QDs) could emit variable fluorescence in the NIR-II window due to their narrow band gap. Unfortunately, the fluorescence in the NIR-IIb region was not strong enough to image in vivo because the lattice defects on the surface of Ag2Te increased non-radiative transitions and decreased fluorescence performance. In this work, we coated a Ag2Te core with a Ag2Se shell to passivate the lattice defects and enhance its fluorescence efficiency. The fluorescence property of the as-prepared Ag2Te/Ag2Se core/shell QDs is more than 10 times stronger than that of Ag2Te. The brighter Ag2Te/Ag2Se QDs showed high-resolution in vivo imaging in the NIR-IIb region to distinguish thin blood vessels up to 66 μm. Overall, Ag2Te/Ag2Se QDs with excellent fluorescence in the NIR-IIb region were prepared and showed great potential for biomedical imaging.
研究领域:
材料科学
·
生物医学工程
·
光学成像
✓ 已验证
产品关联
DOI
高影响因子
Small
·
2021
·Vol. 17
·期 8
·2006111
·
IF 13.281
Mengxuan Yu,Xiaohu Yang,Yejun Zhang,Hongchao Yang,Haoying Huang,Zan Wang,Jinyi Dong,Rong Zhang,Ziqiang Sun,Chunyan Li,Qiangbin Wang
Ag 2 Se quantum dots (QDs) as an effective biological probe in the second near-infrared window (NIR-II, 1000–1700 nm) have been widely applied in bioimaging with high tissue penetration depth and high spatiotemporal resolution. However, the ions deficiency and crystal defects caused by the high Ag + mobility in Ag 2 Se crystals are mainly responsible for the inefficient photoluminescence (PL) of Ag 2 Se QDs. Herein, a tailored route is reported to achieve controllable doping of Ag 2 Se QDs in which Ag is exchanged by Pb via cation exchange (CE), which is unattainable by direct synthetic methods. The Pb-doped Ag 2 Se QDs (denoted as Pb:Ag 2 Se QDs) present fire-new optical features with significantly enhanced PL intensity of 4.2 folds. Photoelectron spectroscopy confirms that Pb acts as an n-type dopant for Ag 2 Se QDs and therefore the electronic impurities provide additional carriers to fill the traps. Moreover, the general validity of this method is demonstrated to convert different sized Ag 2 Se into Pb:Ag 2 Se QDs, so that a wide range of NIR-II PL with high intensity is obtained. The bright NIR-II emission of Pb:Ag 2 Se QDs is further successfully performed in lymphatic system mapping.
研究领域:
生物医学工程
·
材料科学
·
光学成像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Zhiqiang Zhou,Jianfang Cen,Naijia Jiang,Yu Sun,Zhouyang Li,Liyun Yang
With the widespread application of Ag + in modern life and industry, the potential hazardous effects of Ag + to environment and humans have attracted great concerns. Thus, effective and rapid strategies for Ag + detection are highly desirable. In this paper, a novel ratiometric fluorescence sensor using CdSe quantum dots (QDs) has been constructed for sensitive and selective detection of Ag + , which is based on the formation of Ag 2 Se QDs. CdSe QDs were initially prepared and showed single wavelength emission at 510 nm. When Ag + exists, a rising peak appeared at 650 nm and the emission at 510 nm declined, exhibiting distinct ratiometric fluorescence emission ( I 650 / I 510 ) characteristic with a linear response over the Ag + concentration range of 0.01–4 μM. Significantly, the fluorescence changed from green to red. The detection limit of the constructed sensor is 1.4 nM. Furthermore, the sensing assay can be successfully applied to detect Ag + in real water samples and living cells.
研究领域:
分析化学
·
环境科学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）