CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Yu-Xiao Wang,Ting Zhang,Yue Xin,Xiao-Jun Huang,Jun-Yi Yin,Shao-Ping Nie
Agrocybe cylindracea is a common source of active polysaccharides, but their fine structures are not clearly elucidated. In the present study, four fractions were purified from the alkaline extract of A. cylindracea (JACP), and their chemical components and structures were compared by HPAEC-PAD, methylation combined with GC–MS, and 1D/2D NMR analysis. Results showed the purified fractions’ physicochemical properties, including monosaccharide compositions, molecular weights, viscosities and surface morphology considerably varied. JACP-30 was identified as a fucoglucogalactan with a α-(1 → 6)-galactopyranosyl as main chain. JACP-50p and JACP-80r were characterized as β-(1 → 6)-glucans with side chains composed of terminal and 3-substituted β-glucopyranosyl residues attached at O -3 for every three residues. Similarly, the backbone of JACP-80 was β-(1 → 6)-linked glucopyranosyl and β-(1 → 3,6)-linked glucopyranosyl residues at a ratio of 4:1. This work provides more information to the understanding of polysaccharides from A. cylindracea , further guiding its biological researches and developing the application in food and biomedicine industries.
研究领域:
食品科学
·
生物化学
·
药学
✓ 已验证
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2020
·
IF 7.053
Yu-Xiao Wang,Ting Zhang,Xiao-Jun Huang,Jun-Yi Yin,Shao-Ping Nie
Polysaccharide from Agrocybe cylindracea has been demonstrated to exhibit excellently biological functions, but insufficient understanding of its physicochemical property and fine structure limits its wide application and development. In the present study, two water-soluble heteroglycans, a high molecular weight (SACP-40) and a low molecular weight (SACP-80), were isolated from the fruit bodies of A. cylindracea by hot water extraction and gradient ethanol precipitation. There was little difference in their physicochemical properties, including major chemicals and monosaccharide compositions. Rheological measurements revealed SACP-80 was a typical pseudoplastic fluid with shear thinning behavior at the concentration of 3.0%, whereas exhibited a near-Newtonian fluid at lower concentration. SEM observation suggested SACP-80 showed micro-spherical shapes loosely attached to fibrous filament in its surface. Besides, methylation and 1D/2D NMR analysis indicated that SACP-80 was a branched heteroglycan with a backbone composed of β-(1 → 6)-glucopyranosyl, α-(1 → 6)-galactopyranosyl and α-(1 → 2,6)-glucopyranosyl residue at a ratio of 3:1:1. This work provides a theoretical basis for gaining insight into the relationship between the fine structure and viscosity of polysaccharide from A. cylindracea , which could further guide its functional studies and advance the potential application in food and biomedicine industries.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Jin-Jin Liu,Shi-Kang Chen,Xin Wang,Wei-Wei He,Xiao-Xiao Song,Xiao-Jun Huang,Jun-Yi Yin,Shao-Ping Nie
Polysaccharides derived from Agrocybe cylindracea have been demonstrated to exhibit various bioactivities. However, studies on their structural characteristics during the growth process are limited. This study aimed to compare the physicochemical properties and structural characteristics of alkali-extracted polysaccharides from A. cylindracea fruiting bodies (JACP) across four growth stages. Results showed that the extraction yields and protein levels of JACP declined along with the growth of A. cylindracea, while the contents of neutral sugar and glucose increased significantly. However, JACP exhibited structural characteristics similar to those across the four stages. Four polysaccharide subfractions were isolated from each growth stage, including JACP-Et30, JACP-Et50, JACP-Et60, and JACP-Et70. JACP-Et30 from the four stages and JACP-Et50 from the initial three stages were identified as heteroglucans with β-1,3-d-Glcp and β-1,6-d-Glcp residues as main chains, respectively. However, other subfractions were considered as β-1,6-d-glucans containing minor glucuronic acid. These subfractions were predominantly replaced by Glcp residues at the O-3 and O-6 positions. Overall, while JACP exhibited variable physicochemical properties, its structural characteristics remained stable during the growth process, offering new insights into its potential applications in the food and medicinal industries.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Chujing Fu,Kai Ye,Zhichang Qiu,Gaoxing Ma,Shiguo Chen,Hang Xiao
Agrocybe cylindracea polysaccharides (ACP) are bioactive macromolecules with potential prebiotic properties, yet their digestive stability and microbiota-modulating mechanisms remain unclear. This study aimed to systematically evaluate the in vitro digestion and fermentation characteristics of ACP and its interaction with the gut microbiota. The average molecular weight of ACP was determined to be 2.39 × 10 5 Da, and its primary monosaccharides were glucose (53.93 ± 0.65 %) and galactose (27.36 ± 0.17 %), with a significant content of 1,3-Glc linkages. During colonic fermentation, ACP was selectively utilized by gut bacteria, leading to a significant pH reduction and increased production of short-chain fatty acids (SCFAs), particularly acetate, propionate, and butyrate (total SCFAs: 48.7 mM at 24 h). Furthermore, ACP modulated the composition of gut microbiota by enhancing the relative abundance of beneficial bacteria such as Paraprevotella (400-fold increase) and Bacteroides (3.6-fold increase), while suppressing opportunistic pathogens like Fusobacterium and Escherichia . The correlation analysis conducted between SCFAs and gut microbiota indicated the prebiotic potential of ACP, particularly in enriching SCFA-producing bacteria, including Clostridium_sensu_stricto , Gemmiger, Paraprevotella , and Bacteroides . These findings highlight ACP as a structurally stable polysaccharide capable of modulating gut microbiota composition and metabolic activity, supporting its application in functional foods targeting gut health.
研究领域:
食品科学
·
微生物学
·
营养学
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Jin-Jin Liu,Shi-Kang Chen,Hui Luo,Yan Wang,Xiao-Xiao Song,Wei-Wei He,Xiao-Jun Huang,Jun-Yi Yin,Shao-Ping Nie
This study explored the physicochemical properties and structural characteristics of Agrocybe cylindracea polysaccharides at four developmental stages, as well as their dynamic evolution during maturation. Results showed that the polysaccharides from A. cylindracea water extract exhibited similar structural characteristics across all four maturity stages, despite a significant reduction in yields. Four water-soluble heteroglycans, including one high molecular weight (ACPM-Et50-I) and three low molecular weight (ACPM-Et50-II, ACPM-Et60, ACPM-Et80), were isolated from A. cylindracea at each maturity stage. ACPM-Et50-I was identified as branched heterogalactans, while ACPM-Et60 and ACPM-Et80 were branched heteroglucans. However, ACPM-Et50-II was characterized as a branched glucuronofucogalactoglucan at the tide-turning stage but a glucuronofucoglucogalactan at the pileus expansion stage due to the increase of its α-(1 → 6)-D-Gal p . In general, although the structural skeletons of most A. cylindracea heteroglycans were similar during maturation as shown by their highly consistent glycosyl linkages, there were still differences in the distribution of some heteroglucans. This work has for the first time reported a glucuronofucogalactoglucan in A. cylindracea and its dynamic evolution during maturation, which may facilitate the potential application of A. cylindracea in food and biomedicine industries.
研究领域:
真菌学
·
生物化学
·
食品科学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jin-Jin Liu,Yu-Ke Hou,Xin Wang,Wei-Wei He,Xiao-Jun Huang,Jun-Yi Yin,Shao-Ping Nie
Polysaccharides are the important bioactive macromolecules in Agrocybe cylindracea , but their changes are as yet elusive during developmental process. This study investigated the dynamic changes of polysaccharides from A. cylindracea fruiting body water extract at four developmental stages and its structure characteristics. Results revealed that the polysaccharides from A. cylindracea water extract significantly increased at the pileus expansion stage and the increased fraction could be α-glucan. The further purification and identification indicated that this α-glucan was a glycogen. It had typical morphology of β particles with a molecular weight of 1375 kDa. Its backbone comprised α-D-(1 → 4)-Glc p and α-D-(1 → 4,6)-Glc p residues at a ratio of 5:1, terminated by α-D-Glc p residue. Rheological behavior suggested that it was a Newtonian fluid at the concentration of 1 %. In addition, despite both the glycogen and natural starch were composed of d -glucose, they exhibited the entirely distinct Maltese cross characteristic and unique crystalline structure. This study is the first to demonstrate the presence of abundant glycogen in the pileus expansion stage of A. cylindracea , which provides new insights on the change patterns of fungal polysaccharides.
研究领域:
真菌学
·
生物化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）