This study employed ultrasound-assisted extraction to obtain polysaccharides from Albizia julibrissin Durazz. leaf and pod agricultural by-products, with subsequent process optimization. A comparative analysis was then conducted on the physicochemical properties and anticomplementary activity of the isolated polysaccharides derived from two parts. The investigation identified different extraction conditions for leaf and pod, leaf demonstrated maximum polysaccharide yield (1.07 ± 0.20%) at 70°C, 40 mL/g, 50 min and 249 W, contrasting with pod which achieved 8.32 ± 0.27% yield at 70°C, 28 mL/g, 40 min and 201 W. Besides, physicochemical characterization demonstrated distinct molecular weights between leaf (AJLP) and pod (AJPP) polysaccharides (62.55–232.30 kDa) despite shared functional groups. Both polysaccharides contained mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose in varying ratios, while displaying divergent microstructures and excellent thermal stability. In vitro, leaf (AJLP) and pod (AJPP) polysaccharides exhibited potent anticomplementary activity in alternative and classical pathways, respectively. This study established a foundation for developing and utilizing polysaccharide resources from A. julibrissin agro-byproducts, while providing a theoretical basis for their application in complement system overactivation.
Albizia julibrissin flowers (AJF), known as “He Huan Hua,” are widely used as scented tea and herbal medicine in China. During flower maturation, significant changes in metabolite composition occur. To explore the quality alterations of AJF at different development stages, phytochemical assays combined with bioactivity evaluation were conducted. Tight buds exhibited stronger antioxidant and anti-neuroinflammatory activities than fully opened flowers, despite containing lower levels of conventional quality indicators (quercitrin, total flavonoids, and total phenolics). To characterize metabolic dynamics during AJF development, untargeted metabolomics was used and identified 227 differential metabolites, of which 49 showed consistent changes across all three stages. Distinct stage-specific metabolic patterns were observed, with carotenoids and procyanidins enriched in tight buds, anthocyanins and fatty acyls in mature buds, and triterpenoids in fully opened flowers. Moreover, several differential metabolites enriched in tight buds, including lutein, avicularin, procyanidin B2, and ginsenoside Rh 2 , showed associations with anti-neuroinflammatory activity based on in vitro evaluation. In conclusion, stage-dependent metabolic reprogramming contributes to significant quality variation in the development of AJF. These findings provide a foundation for optimizing harvest timing and supporting graded utilization of AJF based on metabolic signatures.
危险品化学品经营许可证（带存储）