INTERNATIONAL BIODETERIORATION & BIODEGRADATION
·
2025
·
IF 4.1
Qingyun Li,Shaohui Yan,Zhengfeng Jiang,Liang Xian,Jianjia Du,Haibo Liu,Youyan Liu
Cyanide and phenol are highly toxic chemicals that exert severe inhibitory effects on microorganisms, posing significant challenges for their removal by biological methods in coexistent systems. In this study, we successfully isolated a bacterial strain named JF101, identified as Alcaligenes faecalis , which was capable of degrading both cyanide and phenol. The interactions between cyanide and phenol during biodegradation were investigated. It was found that the presence of 100 mg/L cyanide significantly enhanced the degradation of phenol, leading to the complete degradation of 1000 mg/L phenol within 86 h. In contrast, only 21.9% of phenol was degraded in the absence of cyanide. Additionally, the presence of phenol had minimal impact on cyanide degradation; a concentration of 100 mg/L cyanide could be rapidly and completely degraded within 40 min even in the presence of up to 1000 mg/L phenol. Five empirical kinetic models of Haldane, Aiba, Edward, Yano, and Webb were employed to describe the degradation processes of phenol and cyanide in both single-substrate and dual-substrate systems. The putative degrading enzymes, degradation products, and pathways associated with the co-degradation of cyanide and phenol were systematically analyzed. The formamide and NH 4 + -N produced from the rapid degradation of cyanide served as nitrogen sources, facilitating the resumed growth of strain JF101 and enhancing phenol degradation.
研究领域:
环境微生物学
·
污染治理
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Ali Mohsin,Jingyun Sun,Imran Mahmood Khan,Haifeng Hang,Muhammad Tariq,Xiwei Tian,Waqas Ahmed,Sobia Niazi,Yingping Zhuang,Ju Chu,Muhammad Zubair Mohsin,Salim-ur-Rehman,Meijin Guo
This study presents an engineered approach for sustainable biosynthesis of curdlan by Alcaligenes faecalis using orange peels. To confirm the substrate suitability a four step study was organized. Firstly, drying of substrate was carried within temperature range of 60–120 °C, along with the application of moisture diffusion control model. Secondly, fermentation medium was obtained via saccharification and detoxification, releasing highest sugar at 72.34 g/L with phenolics removal of 95–98%. Thirdly, curdlan fermentation was conducted in detoxified orange peel hydrolysate followed by optimization of batch culture fermentation via kinetic modeling using Logistic and Luedeking-Piret equations. In 5 L bioreactor, highest specific growth rate ( μ m = 0.233/h), highest curdlan production ( P m = 23.24 g/L) and growth associated rate constant ( α = 3.403) were achieved. Moreover, the total sugar consumption and conversion rates were 83.27% and 53.20%. Lastly, characterization techniques such as FTIR, NMR, XRD, TGA, HPGPC and EDS were applied to biosynthesized curdlan for qualitative validation.
研究领域:
生物工程
·
微生物学
·
可持续发展
✓ 已验证
DOI
PubMed
高影响因子
Environmental Earth Sciences
·
2025
·Vol. 84
·期 12
·1-12
·
IF 2.8
Jiang Chengchen,Zhou Shuai,Shu Xiaohua,Yu Guo,Zeng Honghu,Zhu Zongqiang,Yang Chen,Dang Zhi
Composite pollution poses a significant challenge for soil remediation. This study simulated arsenic and PAH-contaminated soil in karst regions to evaluate the effectiveness of electro-bioremediation under varying soil conditions. Results showed that Alcaligenes faecalis subsp. faecalis exhibited strong PAH degradation and moderate As(V) reduction capabilities. PAH removal efficiency was maximized (55.93%), while arsenic removal efficiency declined as pH increased. Higher soil organic carbon and available nitrogen reduced arsenic removal, whereas increased available phosphorus enhanced it. Under optimized conditions (35 g/kg organic carbon, 150 mg/kg nitrogen, 50 mg/kg phosphorus), residual arsenic was minimized (118.80 mg/kg), and PAH removal peaked at 45.47%. These findings underscore the pivotal role of soil parameters in remediation efficiency, offering practical insights for optimizing electro-bioremediation strategies for soil contaminated by arsenic and PAH.
研究领域:
环境科学
·
微生物学
✓ 已验证
DOI
高影响因子
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
·
2021
·
IF 4.32
Yang Mu,Fuyin Zhang,Ning Li,Shanshan Pi,Ang Li,Qing Hong,Jian He,Jiguo Qiu,Jiandong Jiang
Dipicolinic acid (DPA) is a widely distributed pyridine derivative containing two carboxylate groups. However, the catabolism of DPA mediated by microorganisms are rarely reported. Alcaligenes faecalis strain JQ135, previously isolated in our lab, was able to degrade various pyridine derivatives, including nicotinic acid (NA). In this study, it was found that strain JQ135 could utilize DPA as the sole carbon and nitrogen source for growth and degrade 1.8 mM (300 mg/L) DPA to a non-detectable level within 12 h. The catabolism of DPA by strain JQ135 extracted two intermediates, i.e., 3-hydroxy-2,6-dipicolinic acid and 4-imino-pent-2-enedioic acid. Moreover, deletion of the key genes essential for NA catabolism (such as moaCDEBmoeA and maiA ) did not affect DPA catabolism, indicating that the maleamate pathway and molybdenum cofactor are not involved in the DPA catabolism by strain JQ135. This study will provide insights into the bacterial catabolism of DPA.
研究领域:
微生物学
·
环境科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2026
·
IF 12.2
Su Qingxian,Zhi Mei,Zhong Yijie,Wu Jiapeng,Smets Barth F.,Ng How Yong
The heterotrophic nitrifier Alcaligenes faecalis oxidizes ammonium (NH 4 + ) to dinitrogen gas
(N 2 ) via the key intermediate hydroxylamine (NH 2 OH), accompanied by the formation of nitrite (NO 2 – ), nitric oxide (NO), and nitrous oxide (N 2 O). Although NH 4 + and NH 2 OH oxidation
by A. faecalis were previously attributed
to the direct nitrogen formation ( dnf ) gene cluster,
recent evidence suggests that NH 2 OH oxidation to N 2 proceeds through chemical reactions. However, the exact mechanism
and relative importance of chemical reactions remain unknown. Here,
we characterized the formation of N intermediates in batch incubations
of A. faecalis and determined the kinetics
of NH 2 OH-mediated chemical reactions in abiotic tests under
varying C/N ratios, oxygen, pH, and matrix conditions. Combined with
transcriptomic analyses, we resolved the respective enzymatic and
chemical N turnover pathways across cultivation conditions. Distinct
formation and removal pathways of N intermediates were observed: NH 2 OH was oxidized via both biotic and abiotic processes, with
abiotic contribution accounting for 23–64% of NH 2 OH removal under environmentally relevant conditions (C/N = 8–16,
21% O 2 , pH 7) and increasing to 35–86% at elevated
O 2 (50%) and alkaline pH (9). NO 2 – formation was exclusively enzymatic, whereas N 2 O was
produced predominantly through chemical reactions of NH 2 OH with NO. These findings advance our understanding of inorganic
N metabolism of A. faecalis and highlight
the previously underestimated role of abiotic reactions in N loss.
✓ 已验证
产品关联
DOI
高影响因子
Water
·
2023
·Vol. 15
·期 23
·4084
·
IF 3.4
Zhi Zeng,Jiahui Xiao,Manzhi Li,Jiahui Wu,Taiping Zhang
The effect and mechanism of phenol removal by immobilized microorganisms in Fe3O4nanomaterial-modified pharmaceutical residue biochar was investigated to develop efficient biochar immobilizing microbial technology. Plant residue biochar (Y3, Y5, and Y7) was prepared fromAndrographis paniculataplant residues as the raw material at 300 °C, 500 °C, and 700 °C, respectively. Y7 was modified with Fe3O4nanomaterial (Fe-Y7). These four kinds of biochars were used as carriers to adsorb immobilizedAlcaligenes faecalisstrain JH1, JY3, JY5, JY7, and Fe-Y7 to investigate the mechanism of phenol removal, and eight cycles were performed to analyze their immobilization performance. Compared with suspended bacteria, biochar-immobilized bacteria could improve their tolerance in different environments. At temperatures of 25 °C to 40 °C, pH = 5~9, initial phenol concentration of 300–500 mg/L, and salinity of 3%, the bacteria could still grow and maintain strong activity within 48 h. The water-extractable organic carbon of biochar was also tested for the degradation of phenol by bacteria, which was found to have different stimulating effects on bacteria. In the batch experiments, as the number of cycles increased, the bacteria grew and adhered rapidly to the biochar, eventually forming a thick and sticky biofilm. After the sixth cycle, all the biochar-immobilized bacteria could remove 300 mg/L phenol solution within 12 h. The removal rate of phenol by JFe-Y7 was relatively fast in the eighth cycle. The results indicated that biochar-immobilized bacteria have good durability, stability, and reproducibility and that Fe3O4nanoparticle modification could improve the removal of phenol by increasing the phenol adsorption amount, the adsorption capacity of bacteria, and the enzymatic activity of bacteria.Keywords:biochar;immobilization;bacteria;phenol;mechanism
✓ 已验证
产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Xinyi Li,Tianbo Jia,Haiguang Zhu,Luhan Cai,Yubiao Lu,Jianxin Wang,Hengcong Tao,Peng Li
The CO 2 fixation mechanism by Alcaligenes faecalis ZS-1 in a biocathode microbial fuel cell (MFC) was investigated. The closed-circuit MFC (CM) exhibited a significantly higher CO 2 fixation rate (10.7%) compared to the open-circuit MFC (OC) (2.0%), indicating that bioelectricity enhances CO 2 capture efficiency. During the inward extracellular electron transfer (EET) process, riboflavin concentration increased in the supernatant while cytochrome levels decreased. Genome sequencing revealed diverse metabolic pathways for CO 2 fixation in strain ZS-1, with potential dominance of rTCA and C4 pathways under electrotrophic conditions as evidenced by significant upregulation of the ppc gene. Differential metabolite analysis using LC-MS demonstrated that CM promoted upregulation of various lipid metabolites. These findings collectively highlight that ZS-1 simultaneously generated electricity and fixed CO 2 and that the ppc associated with bioelectricity played a critical role in CO 2 capture. In conclusion, bioelectricity resulted in a significant enhancement in the efficiency of CO 2 fixation and lipid production.
研究领域:
环境科学
·
微生物学
·
生物化学
·
基因组学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Water Process Engineering
·
2023
·
IF 7
Guangxu Bo,Liangcheng Jiao,Huanhuan Li,Guowei Zhao,Zitang Xu,Xiaoxiao Liu,Pengbo Wang,Xiaoke Tian,Jinyong Yan,Xueqing Wang,Yunjun Yan
In this study, the removal efficiencies of bio-based rigid polyurethane foams (“RPUFs” group), Alcaligenes faecalis subsp. faecalis NBRC 13111 T (“strain” group), and the combined use of RPUFs and A. faecalis subsp. faecalis NBRC 13111 T (“RPUFs + strain” group) were investigated. The concentration of NaCl, number of RPUFs, initial pH and initial phenol concentration were sequentially examined. The results showed the adsorption of phenol in “RPUFs” group and “RPUFs + strain” group was improved with the increased number of RPUFs, and the highest adsorption was about 200 mg/L when 40 pieces of RPUFs were used. Under all conditions, the phenol removal efficiency of “RPUFs + strain” group was higher than that of the “strain” group. With the increased concentration of NaCl, the complete removal time of phenol in “strain” group and “RPUFs + strain” group was correspondingly prolonged. In neutral and weakly alkaline conditions, the phenol removal efficiency of “strain” group and “RPUFs + strain” group was much improved, and the phenol-removed time of 500 mg/L of “RPUFs + strain” group was only 30 h at the pH of 7. With the increase of initial phenol concentration, the complete removal time in “strain” group and “RPUFs + strain” group was delayed, especially at the high concentration of 1100 mg/L, the “strain” group had nearly no ability to remove phenol, while the “RPUFs + strain” group could still completely removed phenol at 78 h. The phenol removal mechanism of the “RPUFs + strain” group was attributed to “physical adsorption - biodegradation”.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL RESEARCH
·
2021
·
IF 6.498
Xingyao Ye,Xiaoxian Zheng,Dongqing Zhang,Xiaojun Niu,Yiming Fan,Wangde Deng,Zhang Lin,Huijian Hu,Shaoqi Zhou
Cadmium-contaminated wastewater has attracted increasing concerns due to its non-biodegradable properties and high toxicity. To explore eco-friendly and economically feasible strategies, the screened Alcaligenes faecalis K2 were employed for the biomineralization and recovery of Cd 2+ from wastewater while producing considerable secretory organo-biominerals (SOBs) as bioadsorbents. At 75 mg/L Cd 2+ exposure, 85.5% of Cd 2+ was removed by K2, 43.0% of which was fixed in the granular SOBs. SOBs were convenient for separating from the solution. The adsorption capacity of granular sorbent made from SOBs was verified to be greater than 77.1 mg/g. Practically, 89.5% of 75 mg/L of Cd 2+ could be stably removed while ereK2 continuously generated SOBs in a moving-bed biofilm reactor (MBBR). To sum up, the production of bioadsorbents can be achieved by K2, while removing Cd with live microorganisms , which was conducive to making full use of materials and improving Cd removal efficiency.
研究领域:
环境科学
·
生物技术
·
废水处理
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）