Analytical Methods
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2019
·Vol. 11
·期 33
·4267-4273
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IF 2.378
Miao Xu,Wei Huang,Dingkun Lu,Chunyu Huang,Jingjing Deng,Tianshu Zhou
In this work, a novel dual-sensing probe was developed for detecting an anthrax biomarker (dipicolinic acid, DPA) based on the competitive coordination of Tb3+ between DPA and Alizarin Red (AR). UV-vis titration experiments demonstrated that AR and Tb3+ ions formed a 3 : 1 coordination pair in a boric acid–borate buffer solution. However, the subsequent addition of DPA to the complex solution destroyed the coordination of AR–Tb3+ completely, resulting in the formation of DPA–Tb3+. This constructed a new mechanism of ratiometric colorimetric and fluorescence dual-mode sensing for DPA. By integrating a smartphone with an easy-to-access color-scanning APP, this dual-sensing method could be further applied as a reliable scanometric assay for the rapid and on-site visible detection of DPA directly. With the method established by us, the sensitive and selective detection of DPA released from bacterial spores could be realized easily.
研究领域:
生物化学
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分析化学
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DOI
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
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2019
·Vol. 166
·期 10
·H425
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IF 3.12
Chunxiang Chen,Wen Chen,Jinlong Jiang,Lei Qian,Xue Zhang
In this study, nitrofurazone (NF), a commonly used but banned drug in aquaculture, was used as the research model for nitrofuran antibiotics. A poly-alizarin red modified glassy carbon electrode (PAR/GCE) was prepared by electropolymerization. On this modified electrode, NF can produce a sensitive and stable reduction peak. Other nitrofuran antibiotics, such as nitrofurantoin and furazolidone, showed similar responses. The reduction peak of NF was proportional to the concentration within the range of 3.0–50.0 and 50.0–200.0 μM, and the detection limit was 0.33 μM. The linear ranges of furantoin and furazolidone were 10–40 and 50–140 μM, respectively, with corresponding detection limits of 0.73 and 1.56 μM. The modified electrode was used to detect NF in the cultured water sample, and the recovery rate of the sample was 94.5%–104.8%. Thus, the result was satisfactory.
研究领域:
分析化学
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环境科学
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生物医学
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DOI
高影响因子
Jove-Journal of Visualized Experiments
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2021
·
IF 1.355
Jie Ding,Rui Yan,Luna Wang,Qianlei Yang,Xiaoyun Zhang,Nan Jing,Yuanjie Wei,Hengdong Zhang,Yan An
Chemically induced bone loss due to lead (Pb) exposure could trigger an array of adverse impacts on both human and animal skeletal systems. However, the specific effects and mechanisms in zebrafish remain unclear. Alizarin red has a high affinity for calcium ions and can help visualize the bone and illustrate skeletal mineral mass. In this study, we aimed to detect lead acetate (PbAc)-induced bone loss in zebrafish larvae by using alizarin red staining. Zebrafish embryos were treated with a series of PbAc concentrations (0, 5, 10, 20 mg/L) between 2 and 120 h post fertilization. Whole-mount skeletal staining was conducted on larvae at 9 days post fertilization, and the total stained area was quantified using ImageJ software. The results indicated that the mineralized tissues were stained in red, and the stained area decreased significantly in the PbAc-exposure group, with a dose-dependent change in bone mineralization. This paper presents a staining protocol for investigating skeletal changes in PbAc-induced bone defects. The method can also be used in zebrafish larvae for the detection of bone loss induced by other chemicals.
研究领域:
毒理学
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发育生物学
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环境科学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2012
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IF 4.555
Xi Ba,Liqiang Luo,Yaping Ding,Zhen Zhang,Yuliang Chu,Bijun Wang,Xiaoqian Ouyang
In this work, a poly(alizarin red)/Graphene composite film modified glassy carbon electrode (PAR/Graphene/GCE) was prepared for simultaneous determination of four DNA bases (guanine, adenine, thymine and cytosine) without any pretreatment. The morphology and interface property of PAR/Graphene films were examined by scanning electron microscopy and electrochemical impedance spectroscopy. The PAR/Graphene/GCE exhibited excellent electrocatalytic activity toward purine (guanine and adenine) and pyrimidine (thymine and cytosine) in 0.1 M phosphate buffer solution (pH 7.4). Under optimum conditions, differential pulse voltammetry was used to detect the oxidation of purine and pyrimidine. The results showed that PAR/Graphene/GCE exhibited well-separated peaks, low detection limit, high sensitivity and wide linear range for simultaneous detection of purine and pyrimidine. The proposed sensor also has good stability and reproducibility. Furthermore, the modified electrode was applied for the detection of DNA bases in a fish sperm DNA sample with satisfactory results.
研究领域:
生物化学
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分析化学
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材料科学
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DOI
高影响因子
ELECTROCHIMICA ACTA
·
2014
·
IF 4.086
Shan Liu,Maochao Wei,Xiangli Zheng,Shuai Xu,Fangquan Xia,Changli Zhou
In the paper, a novel and sensitive electrochemical sensor based on modification of electroactive alizarin red S functionalized mesoporous silica material SBA15 onto glassy carbon electrode (ARS-SBA15/GCE) was developed. Alizarin red S , called electrochemical probe that can selectively recognize polycyclic aromatic hydrocarbons (PAHs), as tools for the detection of tricyclic aromatic hydrocarbon anthracene. The morphology and interface property of ARS modified SBA15 (ARS-SBA15) were examined by transmission electron microscopy (TEM) and fourier transform infrared spectroscopy (FTIR). Taking advantage of the π-π stacking force between alizarin red S and anthracene, the ARS-SBA15/GCE sensor could detect anthracene quantitatively in a wide range of 1.0 pM–10.0 nM and a low detection limit of 0.5 pM (S/N = 3). Other PAHs, such as naphthalene, phenanthrene, pyrene, and benzo[a]pyrene show little interference on the detection. Consequently, a simple and sensitive electrochemical method was proposed for the determination of anthracene, which can be used to determine anthracene in waste water samples. The electrochemical method provides a general tool that complements the commonly used spectroscopic methods and immune method for the detection of PAHs.
研究领域:
分析化学
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环境科学
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DOI
高影响因子
IONICS
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2019
·Vol. 26
·期 1
·449-456
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IF 2.289
Zheng Li,Zhang Wen,Guo Jun
A modified electrode Ni(II)–ARS/GR/CPE has been fabricated by electrodepositing nickel(II)–alizarin red complex on the surface of graphene-modified carbon paste electrode (GR/CPE) in alkaline solution. The modified electrode is characterized by scanning electron microscopy, Fourier transform infrared spectrometry, x-ray photoelectron spectroscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. It exhibits the characteristic of improved reversibility and enhanced current responses of the Ni(III)/Ni(II) couple compared with Ni(II)–ARS/CPE. It also shows enhanced electrocatalytic activity toward the oxidation of formamide, which is due to the high surface area and facile charge–transfer ability of graphene and Ni(II)–ARS complex. The mechanisms of film formation and the catalytic process are also discussed. Moreover, the catalytic currents present linear dependence on the concentration of formamide from 0.5 mM to 0.21 M by amperometry; the detection limit is 0.15 mM. The modified electrode for formamide determination is of the property of simple preparation, good stability, fast response, and acceptable sensitivity.
研究领域:
分析化学
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电化学
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DOI
高影响因子
APPLIED CATALYSIS A-GENERAL
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2021
·
IF 5.706
Qingrui Peng,Haijun Wang,Yongmei Xia,Xiang Liu
A multifunctional Zr-containing catalyst (FM-Zr-ARS) was successfully synthesized by a modulated hydrothermal synthesis route. Systematic characterization results supported the presence of robust porous inorganic-organic frameworks stabilized by the strong coordination interaction of Zr 4+ with oxygen-rich functional groups in Alizarin red S (ARS). Moreover, the -O-Zr-O- network in the FM-Zr-ARS structure formed a rich content of acid-base sites. In addition, the inherent sulfonic groups in ARS made the FM-Zr-ARS hybrids possess Brønsted acid sites. Therefore, under the synergistic catalysis of the multiple functional sites, FM-Zr-ARS showed remarkably high catalytic activity for γ-valerolactone (GVL) production from levulinate esters and furfural. Finally, 72.4 % and 97.7 % yields of GVL were obtained in the conversion of furfural and ethyl levulinate, respectively, after 8 h of reaction at 433 K. On the basis of the role of different functional sites, a plausible catalytic mechanism was presented for the conversion of biomass-derived furfural to GVL.
研究领域:
化学工程
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生物质转化
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2026
·
IF 10.6
Shaohui Wei,Chengtao Nong,Zonkui Qin,Menglei Gong,Shiqiang Li,Guangzhan Lu,Fukang Deng,Jianbin Li
Post-equilibrium displacement induced secondary release of pre-adsorbed dye. • Congo Red remobilized pre-adsorbed Alizarin Red S in binary systems. • A clear CR > ARS competitive retention hierarchy drove asymmetric displacement. • DFT/IGMH supported stronger multi-site interfacial interactions of Congo Red. • Competitive displacement tests improve adsorbent risk evaluation. Competitive adsorption in multicomponent wastewater can induce post-equilibrium displacement and remobilization of previously adsorbed pollutants. Here, CCS@NH 2 -MWCNTs was used as a model composite interface to investigate the competitive adsorption and displacement of Congo Red (CR) and Alizarin Red S (ARS). CR exhibited stronger competitive retention than ARS: CR displaced 59.90% and 64.87% of pre-adsorbed ARS at 30 and 40 °C, respectively, whereas reverse displacement remained below 1% at 30 °C and no measurable CR release was detected at 40 °C. Preferential CR retention was maintained over an initial pH range of 4–10. Spectroscopic characterization and DFT/IGMH analyses suggest that charge distribution, extended π-conjugation, multi-site interactions, and interfacial accessibility collectively contribute to the observed retention hierarchy. These findings show that apparent adsorption equilibrium does not necessarily ensure persistent contaminant immobilization and highlight post-equilibrium displacement as a potential secondary-release mechanism relevant to multicomponent wastewater treatment. Download: Download high-res image (294KB) Download: Download full-size image
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DOI
高影响因子
ELECTROCHIMICA ACTA
·
2019
·
IF 5.383
Fen Liu,Xianwen Kan
In this work, a novel dual-analyte electrochemical sensor was developed based on the combination of boronate affinity and indicator displacement assay (IDA) strategy. Reduced graphene oxidation (RGO) and poly(3-aminophenylboronic acid) (pAPBA) were modified on glassy carbon electrode (GCE), which further bound dopamine (DA) via a reversible covalent interaction between the APBA and DA. Due to the higher affinity of pAPBA to the analytes, the bound DA can be displaced by fructose (Fru) or Alizarin Red S (ARS). As an indicator, the decrease of DA current caused by the displacement was used to monitor the concentration of the analyte. For non electro-active molecule, the decrease of DA current was directly proportional to the logarithm of Fru concentration in a range from 7.0 × 10 −8 to 1.0 × 10 −4 mol/L. For electro-active molecule, the current of DA decreased along with the increase of ARS current owing to the displacement, which were combined as a dual-signl for ARS sensitive detection with a linear range of 9.0 × 10 −8 - 1.0 × 10 −5 mol/L. The results of determining analytes with their interferences demonstrated the good specific affinity of the sensor toward the analytes. The dual-analyte electrochemical sensor based on IDA strategy probably provides a potential guidance for multi-analyte facile, sensitive, and specific detection.
研究领域:
生物化学
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分析化学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
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2014
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IF 5.825
Lu-Liang Wang,Juan Qiao,Hui-Hui Liu,Jie Hao,Li Qi,Xiao-Ping Zhou,Dan Li,Zong-Xiu Nie,Lan-Qun Mao
Glucose monitoring with high sensitivity and accuracy in the cerebrospinal fluid is a challenge for evaluating the role of glucose in the physiological and pathological processes. In this work, a ratiometric fluorescent probe for sensing glucose was developed. In the probe, the gold nanoclusters protected by ovalbumin played the role as the reference of fluorophore and the Alizarin Red S-3-aminophenyl boronic acid immobilized on the poly(N-acryloxysuccinimide) acted as both the response signal and specific recognition unit for sensing glucose. Once the ratiometric fluorescent probe reacted with glucose in the biological system, its fluorescence intensity at 567 nm was quenched, while the fluorescence intensity at 610 nm was essentially unchanged. In addition, the prepared ratiometric fluorescent probe showed higher stability against environmental effects. As a result, the present ratiometric fluorescent probe was successfully used for monitoring of glucose in the rat brain following the cerebral calm/ischemia.
研究领域:
分析化学
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生物医学工程
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神经科学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2021
·
IF 4.821
Xiaojing Si,Luchen Deng,Yingzi Wang,Mei Han,Yaping Ding
A novel poly alizarin red (AR) film incorporating carboxylic acid group functionalized multiwall carbon nanotube (f-MWCNT) modified glassy carbon electrode (GCE) was first prepared for the determination of luteolin (Lut). Scanning and transmission electron microscopy were applied for characterization of the surface morphology of the modified electrodes and cyclic voltammetry was used to investigate the electrochemical properties of the proposed electrode towards the oxidation of Lut. Optimization of the experimental parameters was performed with regard to pH, drop-coating volume of f-MWCNT, electro-polymerization cycles of alizarin red, and different potentials of i-t. And the mechanism for Lut detection based on AR/f-MWCNT/GCE was investigated by electrochemical methods. The linearity between the oxidation peak current and the Lut concentration was obtained in the range of 0.5 to 45 μM with a detection limit of 0.17 μM (S/N = 3). In addition, the AR/f-MWCNT/GCE electrode displayed high selectivity, good stability and reproducibility, and also successfully determined Lut in drug samples.
研究领域:
分析化学
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电化学
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纳米技术
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药物分析
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产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2022
·
IF 7.336
Quanlu Wang,Yikun Wang,Tingting Zhang,Yanmin Wang,Qiang Zhang,Tingxi Li,Yongqin Han
Metal-organic frameworks (MOFs) are promising candidates for supercapacitors due to their intrinsic porous structure. However, their low conductivity limits their supercapacitive performances. Herein, carbon cloth/ZIF67/polypyrrole composites (CZPAs) are prepared by first in-situ growing ZIF67 on carbon cloth and then electrochemical polymerizing pyrrole on carbon cloth/ZIF67 utilizing alizarin red S (ARS) as redox dopant. The synergistic effects of ZIF67 and ARS including porous structure, doping effect and inducing limited/ordered growth of polyprrole are contributed to the improved electrochemical performances. The resulted composite doped by 35 mM ARS (CZPA-35) achieves the highest areal capacitance of 1198 mF cm −2 among the CZPAs. The asymmetric supercapacitor assembled by CZPA-35 and carbon cloth/active carbon as flexible electrodes demonstrates high areal capacitance (288 mF cm −2 ), high energy density (57.6 μWh cm −2 ) and power density (300.96 μW cm −2 ). Moreover, the flexible supercapacitor provides excellent cycle stability after 5000 cycles (87.1% capacitance retention) and flexibility (85% capacitance retention after bending angle of 180 °). This strategy can be extended to fabricate other kinds of flexible MOF/PPy composites for the potential application of flexible wearable and portable electronic products.
研究领域:
材料科学
·
能源存储
·
电化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）