Journal of Nanotechnology
·
2020
·Vol. 2020
·期 1
·8828878
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IF 0
Theint Theint Win,Sikandar Khan,PengCheng Fu
The scientific consensus is now on developing a biocontrol agent that can cause cellular metabolic reprogramming against agricultural pathogens. Biosynthesis of silver nanoparticles was performed by using phytopathogenic fungi ( Alternaria sp.) isolated from banana cultivated soil. Alternaria sp. can grow very fast and produce high enough bioactive compounds. This study aims to biosynthesize silver nanoparticles (AgNPs) using fungal Alternaria sp.’s metabolites as a safe antifungal agent against plant pathogenic fungi ( Fusarium spp. and Alternaria sp.). To visualize the formation of AgNPs, analytical instruments were used, such as ultraviolet-visible (UV-Vis) spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, scanning transmission electron microscopy (STEM), energy dispersive X-ray (EDX), and elemental mapping. The UV-visible spectra showed a peak at 435 nm. Analysis of scanning transmission electron microscopy (STEM) micrographs evidenced that the size of synthesized silver nanoparticles ranged between 3 and 10 nm. The resulting AgNPs showed distinct antifungal activity against selected plant pathogenic fungi. Synthesized AgNPs have demonstrated remarkable potential for the use of antifungal compounds to combat plant diseases.
研究领域:
微生物学
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纳米生物学
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植物病理学
✓ 已验证
DOI
PLANTA MEDICA
·
2020
·
IF 2.687
Ying Gao,Jia Zhou,Hanli Ruan
Three new (alterchothecenes A – C, 1 –3) and 3 known (4 –6) trichothecenes, along with 9 known compounds (7 –15), were isolated from the culture of Alternaria sp. sb23, an endophytic fungus separated from the root of Schisandra sphenanthera Rehd. et Wils. Their structures were elucidated by spectroscopic analyses, and the absolute configurations of 1–3 were determined through comparison of the experimental electronic circular dichroism (ECD) spectra and optical rotations with similar analogues. In vitro cytotoxicity tests of compounds 1–6 against human HT-29 colon carcinoma and human MCF-7 breast cancer cell lines indicated that 4–6 exhibited significant cytotoxic effects, with IC50 values ranging from 0.89 to 9.38 µM. And the potential of compounds 1–6 as tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) sensitizers in HT-29 cells was evaluated. The results revealed that combination treatment of TRAIL with compounds 1–6 synergistically decreased cell viability compared with the sole treatment with those compounds.
研究领域:
药理学
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化学
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肿瘤学
·
真菌学
✓ 已验证
DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2013
·Vol. 97
·期 23
·9941-9954
·
IF 3.689
Zhang Jinhua,Shi Junling,Liu Yanlin
To identify the substrates and enzymes related to resveratrol biosynthesis in Alternaria sp. MG1, different substrates were used to produce resveratrol, and their influence on resveratrol production was analyzed using high performance liquid chromatography (HPLC). Formation of resveratrol and related intermediates was identified using mass spectrum. During the biotransformation, activities of related enzymes, including phenylalanine ammonia-lyase (PAL), trans -cinnamate 4-hydroxylase (C4H), and 4-coumarate-CoA ligase (4CL), were analyzed and tracked. The reaction system contained 100 mL 0.2 mol/L phosphate buffer (pH 6.5), 120 g/L Alternaria sp. MG1 cells, 0.1 g/L MgSO 4 , and 0.2 g/L CaSO 4 and different substrates according to the experimental design. The biotransformation was carried out for 21 h at 28 °C and 120 rpm. Resveratrol formation was identified when phenylalanine, tyrosine, cinnamic acid, and p -coumaric acid were separately used as the only substrate. Accumulation of cinnamic acid, p -coumaric acid, and resveratrol and the activities of PAL, C4H, and 4CL were identified and changed in different trends during transformation with phenylalanine as the only substrate. The addition of carbohydrates and the increase of phenylalanine concentration promoted resveratrol production and yielded the highest value (4.57 μg/L) when 2 g/L glucose, 1 g/L cyclodextrin, and phenylalanine (4.7 mmol/L) were used simultaneously.
研究领域:
微生物学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
NATURAL PRODUCT RESEARCH
·
2023
·
IF 2.2
De-Wen Bi,Jian- Feng,Wen-Hui Pang,Peng-Yun Yang,Yao-Jun Xu,Muhammad Aurang Zeb,Meng-Ru Wang,Xing-Jie Zhang,Xiao-Li Li,Rui-Han Zhang,Wei-Guang Wang,Wei-Lie Xiao
Three new lanostane triterpenoids (1-3) along with two new amides fatty compounds (4-5) were isolated from the ethyl acetate extract of a culture of the endophytic fungus Alternaria sp. gx-2. Their structures were identified by 1D and 2D NMR spectral data and HRESIMS. Compounds 1-12 were evaluated for their anti-inflammatory and tyrosinase inhibition activities. The isolated compounds did not show inhibitory activities at a concentration of 100 μM against tyrosinase, while under the concentration of 10 μM, the release of lactate dehydrogenase (LDH) inhibition rate of compound 1 was 54.45%, indicating that compound 1 had moderate anti-inflammatory activity on the activation of NLRP3 inflammasome.
研究领域:
药理学
·
天然产物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BMC MICROBIOLOGY
·
2024
·Vol. 24
·期 1
·1-12
·
IF 4
Yang Wei-Kang,Gong Zhu,Wang Bao-Teng,Hu Shuang,Zhuo Ye,Jin Chun-Zhi,Jin Long,Lee Hyung-Gwan,Jin Feng-Jie
With the development of industry and modern manufacturing, nondegradable low-density polyethylene (LDPE) has been widely used, posing a rising environmental hazard to natural ecosystems and public health. In this study, we isolated a series of LDPE-degrading fungi from landfill sites and carried out LDPE degradation experiments by combining highly efficient degrading fungi in pairs. The results showed that the mixed microorganisms composed of Alternaria sp. CPEF-1 and Trametes sp. PE2F-4 (H-3 group) had a greater degradation effect on heat-treated LDPE (T-LDPE). After 30 days of inoculation with combination strain H-3, the weight loss rate of the T-LDPE film was approximately 154% higher than that of the untreated LDPE (U-LDPE) film, and the weight loss rate reached 0.66 ± 0.06%. Environmental scanning electron microscopy (ESEM) and Fourier transform infrared spectroscopy (FTIR) were used to further investigate the biodegradation impacts of T-LDPE, including the changes on the surface and depolymerization of the LDPE films during the fungal degradation process. Our findings revealed that the combined fungal treatment is more effective at degrading T-LDPE than the single strain treatment, and it is expected that properly altering the composition of the microbial community can help lessen the detrimental impact of plastics on the environment.
CAS / MDL:
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED MICROBIOLOGY
·
2021
·Vol. 131
·期 4
·1890-1908
·
IF 3.772
Win T.T.,Bo B.,Malec P.,Fu P.
AimsThis study sought to utilize indigenous soil micro‐organisms to suppress wilt‐causing fungal pathogens of the banana.Methods and ResultsFungal pathogens were isolated from wilt‐affected rhizospheric soil, and potential antagonistic bacterial strains were isolated from healthy rhizospheric soil in the same area from which fungal pathogens were isolated. The antifungal activity of isolated micro‐organisms against fungal pathogens was studied both in vitro and in vivo against fungal pathogens. It was found that Fusarium oxysporum and Alternaria sp. were pathogenic, while Penicillium sp., Bacillus velezensis and Bacillus subtilis were antagonistic. Moreover, it was seen that B. velezensis, B. subtilis and Penicillium sp. inhibited the growth of the two fungal pathogens in both in vitro and in vivo experiments. Further investigation indicated that B. velezensis, B. subtilis and Penicillium sp. were able to produce enzymatic antifungal compounds (chitinase and β‐1,3‐glucanase). The spray application around rhizome revealed that a combination of Bacillus spp. and Penicillium sp. in greenhouse conditions gave the highest reduction in disease severity by up to 60% to both fungal pathogens among the treatments.ConclusionsBanana disease is seen to be induced not only by F. oxysporum but also by Alternaria sp. The isolated indigenous micro‐organisms can effectively control both the pathogens. The combination of isolated antagonistic micro‐organisms has thus demonstrated substantial potential for suppressing banana disease.Significance and Impact of the StudyAn antagonistic consortium isolated in this study has demonstrated remarkable potential for controlling fungal diseases caused by Fusarium sp. and Alternaria sp. Therefore, the use of indigenous microflora to improve disease suppression of banana plants against soil‐borne pathogens is a preferable approach.
研究领域:
微生物学
·
植物病理学
·
农学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）