Marine biofouling, the accumulation of microorganisms, algae, and invertebrates on submerged surfaces, poses significant challenges for maritime industries. This study introduces a new biocide specifically designed to combat marine biofouling and assesses its efficacy and underlying algicidal mechanisms. The results showed that the biocide LaPT, synthesized by combining the rare-earth element Lanthanum (La) with pyrithione (PT), exhibited not only remarkable antibacterial properties but also strong algicidal activity against diatom species Amphora sp. and Thalassiosira pseudonana . Long-term field tests conducted in Xiamen Port, confirmed the effectiveness of LaPT-coated panels in reducing barnacle infestation. Algicidal mechanistic investigations through physiological, transgenic, and transcriptomic analyses revealed that LaPT disrupts cell wall and membrane integrity, interferes with calcium-dependent processes, and inhibits photosynthesis and energy metabolism, leading to diatom cell death. This study demonstrates the LaPT's effectiveness in preventing biofouling and reveal its unique mechanism of action, offering a promising solution for managing marine biofouling.
JIN CUILI,YU ZHAOWEI,PENG SHUYA,FENG KE,ZHANG LIKUI,ZHOU XIAOJIAN
Exopolysaccharide (EPS) of two benthic diatoms, Amphora sp. and Stauroneis sp., with different biofilm formation abilities were investigated. The ratio of suspension-cells/biofilm-cells was employed to indicate the diatom biofilm formation abilities. The soluble EPS from the supernatant of whole culture, tightly bound EPS from floating cells, loosely and tightly bound EPS from biofilm cells were fractionated as SL-EPS, F-TB-EPS, BF-LB-EPS and BF-TB-EPS, respectively. The analysis for productions and monosaccharide compositions indicated that EPS from two diatoms were different in terms of the productions, distributions, and monomer compositions. Amphora sp. produced more (1.5-fold) total exopolysaccharides, but less (<0.4-fold) BF-TB-EPS than Stauroneis sp. The monosaccharides of the EPS from Amphora sp. were more diverse than those of Stauroneis sp., with 13 and 10 monomers, respectively. Neutral sugars, Glc, Xyl and Man, were abundant in Stauroneis sp., while Gal, Glc and Xyl were rich in Amphora sp. Uronic acid and hexosamine were present in all fractions of two diatoms, especially Glc-A being the most abundant monomer in SL-EPS of Amphora sp. It was proposed that the high content of uronic acid (especially Glc-A) might be crucial for the strong biofilm formation abilities of Amphora sp.
危险品化学品经营许可证（带存储）