Chinese Journal of Natural Medicines
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2018
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IF 1.991
Qi-Qing LI,Jian ZHANG,Ji-Hua LIU,Bo-Yang YU
Andrographis paniculata (Burm. f.) Nees (AP) is commonly used for the treatment of many infectious diseases and has been cultivated widely in Asian countries, and has been included in United States Pharmacopoeia as a dietary supplement, but the cultivars of A. paniculata are not abundant due to its self-pollinated. With the aims to enrich AP resources and provide materials for after breeding we explored the polyploidy induction. Different explants, colchicine concentration, and treatment time were tested. After identification by flow cytometry, eleven polyploid plants with different morphologic traits were obtained. The agronomic traits and andrographolide concentration of the polyploids were improved greatly. One of the polyploids (serial 3–7) was chosen for further study. The traits of the second and third generation polyploids (serial 3–7) were stable. Compared with the normal plants, the seeds (2nd generation) weight increased by 31%, and the andrographolide concentration of the leaves increased by 14% (2nd) and 28% (3rd). In conclusion, AP autopolyploids with different morphologic traits were established successfully for the first time, and the polyploids induction might be effective for crop improvement of AP.
研究领域:
植物学
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药用植物研究
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遗传学
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PubMed
高影响因子
Frontiers in Chemistry
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2022
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IF 5.545
Jiang Zhehui,Wang Tianhao,Zhang Kaixue,Zhang Meiqi,Ma Ling,Ren Weichao,Bao Yihong,Ma Wei
Light quality consists of a spectrum of different bands, which not only affects plant, development, and primary metabolism but also affects the secondary metabolism of plants. It is an important factor affecting the content of active components of medicinal plants. The A. paniculata seedlings planted in the laboratory, as materials, were tested with red light, far red light, blue light, and ultraviolet light separately. The study assays the content of six main chemical components separately by LC-MS, observes the changes in the content, and analyzes the relationship between the light quality and the active ingredient of A. paniculata. Using the ointment yield and pH value, the fingerprint analysis method of A. paniculata standard decoction was established, and we discussed the selection of index components of A. paniculata standard decoction. It was suggested to select andrographolide as the index component. It will provide a theoretical basis for the large area cultivation of A. paniculata and optimize the quality of medicinal materials to ensure the quality of standard decoction.
研究领域:
生药学
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植物生理学
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药物化学
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PubMed
高影响因子
FITOTERAPIA
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2012
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IF 1.848
Yonglei Yuan,Long Ji,Liping Luo,Juan Lu,Xiaoqiong Ma,Zhongjun Ma,Zhe Chen
In the present study, it was demonstrated that the petroleum extract of Andrographis paniculata (AP) had quinone reductase (QR) inducing activity, which might be attributed to the modification of key cysteine residues in Keap1 by Michael addition acceptors (MAAs) in it. To screen MAAs in AP, glutathione (GSH) was employed, and a LC/MS/MS method was implied. Three compounds, andrographoside, andrographolide , 14-deoxy-14,15-dehydroandrographolide were revealed could well conjugated with GSH. Then, andrographolide along with 4 new and 14 known compounds were isolated to conduct QR induction evaluation, and the CD (the concentration required to double the activity of QR) value of andrographolide is 1.43 μM. The QR induce activity of andrographolide might be attributed to its targeting multiple cysteine residues in Keap1, therefore, the alkylation of Keap1 by andrographolide was further studied and the result showed that four cysteine residues: Cys77, Cys151, Cys273 and Cys368 were alkylated, which indicated that Keap1 is a potential target for the QR induce activity of andrographolide.
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Antioxidants
·
2025
·Vol. 14
·期 1
·118
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IF 6.6
Heng Xu,Shiying Lan,Simin Lin,Anjing Wang,Yuanlin Luo,Jing Wang,Zhenzhong Yang
Andrographis paniculatais mainly used to treat skin inflammations, wounds, and infections. In this study, Andrographis Herba, the aerial part of the plant, was proven to increase the viability of UVB-damaged HaCat cells and reduce reactive oxygen species levels. The chemical composition of Andrographis Herba extract (AHE) was analyzed using UPLC-Q-TOF-MS, and diterpene lactones were identified as its primary constituents. Then, the fraction of diterpene lactones was prepared and exhibited similar effects to AHE. AHE, its diterpene lactones component, and its representative constituent andrographolide all decreased the expression of IL-1β, IL-6, and CDKN1A. Furthermore, the protective effects of AHE and its active ingredients on UVB-damaged epidermal stem cells were investigated. Notably, the combined treatment with andrographolide and collagen XVII enhanced the viability of UVB-damaged epidermal stem cells, increased the expression of stemness markers integrin β1 and p63, and decreased the expression of the differentiation marker keratin 10. This combination demonstrated significant synergy in maintaining skin homeostasis, which provides evidences for the development of skin-protective products.
研究领域:
药理学
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皮肤生物学
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植物化学
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PubMed
高影响因子
BMC Complementary Medicine and Therapies
·
2023
·Vol. 23
·期 1
·1-16
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IF 3.9
Ma Jianping,Zheng Miao,Zhang Xinyue,Lu Jiaqi,Gu Lili
Background Alzheimer’s disease (AD) is the most prevalent neurodegenerative and remains incurable. Aluminum is a potent neurotoxin associated with AD. The main pathological features of AD are extracellular amyloid-β protein deposition and intracellular hyperphosphorylated Tau protein. A body of evidence suggest that oxidative stress and autophagy are involved in the pathogenesis of AD. Andrographis paniculata (AP) is a native plant with anti-inflammatory, anti-oxidative stress, and regulation of autophagy properties. AP significantly alleviated cognitive impairments, reduced Aβ deposition and has neuroprotective effect. However, its effects on aluminum-induced AD model have not been studied much. In this study, we investigated whether AP protect against aluminum-induced neurotoxicity through regulation of p62-Kelch-like ECH-associated protein 1(Keap1)-Nuclear factor E2 related factor 2 (Nrf2) pathway and activation autophagy in vivo and in vitro.Methods UPLC-ESI-qTOF-MS/MS was used to identify the chemical constituents of AP ethanol extract. The mice with cognitive deficit were established by injecting aluminum chloride and D-galactose, and treated with either AP extract (200, 400, or 600 mg/kg/d) or andrographolide (2 mg/kg/2d).The spatial memory ability was detected by Morris water maze, HE staining were used to detect in brain tissue,Oxidative stress indexs and SOD activity in both serum and brain tissue were detected by kit.The expression of p62-Nrf2 pathway proteins were measured via western blotting. Furthermore, the neurotoxicity model was induced by aluminum maltolate (700 µM) in PC12 cells. Following AP and andrographolide treatment, the cell viability was detected. The relevant mRNA and protein expressions were detected in cells transfected with the p62 siRNA.Results The main active components of AP included andrographolide, neoandrographolide and deoxyandrographolide as identified. AP and andrographolide significantly improved the spatial memory ability of mice, attenuated pathological changes of hippocampal cells, reduced the level of malondialdehyde, and increased superoxide dismutase activity in serum or brain tissue as compared to model control. In addition, the Nrf2, p62 and LC3B-II proteins expression were increased, and p-Tau and Keap1 proteins were decreased in the hippocampus after AP and andrographolide treatment.Furthermore, AP increased aluminum maltolate-induced cell viability in PC12 cells. Silencing p62 could reverse the upregulation expression of Nrf2 and downregulation of Keap1 and Tau proteins induced by AP in aluminum maltolate-treated cells.Conclusion sAP had neuroprotective effects against aluminum -induced cognitive dysfunction or cytotoxicity, which was involved in the activation of the p62-keap1-Nrf2 pathway and may develop as therapeutic drugs for the treatment of AD. However, this study has certain limitations, further optimize the protocol or model and study the molecular mechanism of AP improving AD.
研究领域:
神经科学
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药理学
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分子生物学
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毒理学
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高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2025
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IF 5.4
ZiLiang Chen,ShuLin Luo,JingLu Hu,Xu Zhan,Yanlin Tan,Jing Leng,BoJie Lin
Ethnopharmacological Relevance Andrographis paniculata (Burm.f.) Wall. ex Nees has been used in Thai traditional medicine for topical hair growth promotion. This study investigates andrographolide(AP), a major bioactive compound from Andrographis paniculata, and its underlying mechanism. Aim of the Study To evaluate the effect of AP on hair follicle growth and its regulation via the Wnt/β-catenin pathway. Materials and Methods Mouse vibrissae and human hair follicles were cultured with AP (0–10μM) or minoxidil (10 μM). Hair shaft elongation and anagen duration were recorded. In vivo, depilated C57BL/6 mice received topical AP (1 or 5 mg/mL), with minoxidil as control. Follicular morphology was assessed by H&E staining; Ki67 expression was used to evaluate proliferation. Wnt/β-catenin signaling was analyzed by qRT-PCR and Western blot. Molecular docking was performed to assess potential interactions between AP and key pathway proteins. Results AP significantly promoted hair shaft elongation and prolonged the anagen phase. At 5 μM, its efficacy was comparable to 10 μM minoxidil. In vivo, AP increased follicular density and Ki67-positive cells. Mechanistically, it upregulated Wnt family member 10b (Wnt10b), β-catenin, and lymphoid enhancer-binding factor 1 (LEF1), indicating activation of the canonical Wnt/β-catenin signaling pathway. Molecular docking analysis indicated that AP may bind to Glycogen Synthase Kinase 3 Beta(GSK3β) with a favorable energy of -8.4 kcal/mol. Conclusions AP, derived from Thai traditional hair care, promotes hair growth via follicular proliferation and Wnt/β-catenin activation. In silico data hint at GSK3β as a putative target, offering a mechanistic lead. This supports its development as a natural alopecia therapeutic, pending further validation.
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高影响因子
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