INDUSTRIAL CROPS AND PRODUCTS
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2023
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IF 5.9
Liangliang Han,Libo Zhang,Yuedong He,Lijing Liao,Junde Li,Sheng Xu,Yucheng Zhao,Xiaohong Bian,Yuanzheng Xia
As a popular medicinal plant , Angelica dahurica has been attached to great importance in herbal medicine . Its main active compounds are furanocoumarins (FCs) including imperatorin and isoimperator which exhibit anti-oxidant, anti-tumor, and anti-virus activities. However, the enzymes catalyzing the crucial step of FCs’ biosynthesis pathway remain unidentified. This study initially screened six prenyltransferases (PTs)-coding sequences through transcriptome and gene expression profile analysis. Combined with qRT-PCR and in vitro enzyme activity verification, two C-PTs ( Ad PT1, 2) which convert umbelliferone to 7-demethylsuberosin (DMS), and one O-PT ( Ad PT3) which transforms xanthotoxol to imperatorin were identified. Among these PTs, Ad PT2 also produces a trace amount of osthenol from umbelliferone. Homologous modeling, molecular docking , and site-directed mutagenesis analysis were also conducted and it is discovered that Asp301 and Asp305 in the two Asp-rich regions are vital for the activity of Ad PT1. The study is the first to conduct a frontier exploration of the biosynthesis pathways of FCs in A. dahurica and will establish a foundation for the molecular mechanism of further C-PTs and O-PTs evolutionary studies. Data and materials availability All data needed to evaluate the conclusions in this paper are present in the paper and/or in supplementary. the accession numbers of Ad PT1–3 are OP753387-OP733389, the raw RNA sequence reads are available as SRP289220.
研究领域:
植物学
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分子生物学
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药用植物研究
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DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
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2022
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IF 3.388
Xinyue Pang,Yongshuai Jing,Pengyue Li,Xiaoyue Qiu,Yuguang Zheng,Qian Wang,Lanfang Wu
The study aimed to select the best extraction technology of Angelica dahurica polysaccharides (ADP), characterize its structure, and antioxidant activity. On the basis of the single-factor experiment result, the ultrasonic-assisted extraction (UAE) of ADP was ameliorate using the response surface method. The optimal conditions were: extraction time 32 min, extraction temperature 74°C, ratio of water to material 19 mL/g, extraction power 270 W. The practical extraction rate was 11.21 ± 0.12% (g/g). C-1: Not clear?? g/s??? ADP was purified by column chromatography, and then three polysaccharide fractions (ADP-H, ADP-0.1 and ADP-0.3) were obtained using DEAE-Cellulose 52. The ADP was mainly composed of D-Mannose, D-Glucose, D-Galactose, L-Arabinose, the ADP-H was mainly composed of D-Mannose, D-Glucose, D-Galactose, L-Arabinose, the ADP-0.1 was mainly composed of D-Mannose, D-Galacturonic acid, D-Glucose, D-Galactose, L-Arabinose, and the ADP-0.3 was mainly composed of D-Mannose, L-Rhamnose, D-Galacturonic acid, D-Galactoe, L-Arabinose. The molecular weights of ADP-H, ADP-0.1 and ADP-0.3 were 1.01 × 104 Da, 1.45 × 107 Da and 6.96 × 106 Da. In addition, the purified polysaccharides have certain scavenging ability of DPPH free radical, hydroxyl free radical and ferric reducing power, which can be used as natural products of antioxidants.
研究领域:
植物化学
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药理学
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分析化学
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DOI
高影响因子
Heliyon
·
2024
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IF 3.4
Aitong Yang,Junyan Zhang,Guangying Lv,Jiabao Chen,Long Guo,Yan Liu,Yuguang Zheng,Lei Wang
Angelica dahurica is a medicinal herb of the Umbelliferae family. The dried root of A. dahurica , also known as Angelicae dahuricae Radix, is widely used in clinical treatment. However, the aboveground part of A. dahurica which accounted for over 70% of the total plant was abandoned in the field. In order to develop the value of the aboveground part of A. dahurica , the chemical constituents and arginine kinase (AK) inhibitory activity of A. dahurica leaves were studied. 85 volatile components were identified from A. dahurica leaves by GC-MS; 39 non-volatile components including sugars, amino acids and organic acids were identified by pre-column derivatization GC-MS analysis; and 7 coumarins were qualitatively and quantitatively analyzed by HPLC. Then, an inhibitory enzyme-linked immunosorbent assay (iEIA) was applied for evaluation of AK inhibitory activity. The extracts of A. dahurica leaves exhibited well inhibitory effects on AK. Further, potential AK inhibitors were screened by grey relational analysis and their inhibitory activities were validated by iEIA. l -aspartic acid exhibited strongest inhibitory effect on AK with its IC 50 value was 0.558 mM, which was much lower than that of chlorpheniramine (6.644 mM). The obtained chemical profiles displayed chemical diversity of A. dahurica leaves and will provide data support for the future development and utilization of A. dahurica leaves. The screened potential AK inhibitors from A. dahurica leaves could be candidates for development of antiallergic substances or insecticides.
研究领域:
药理学
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植物化学
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DOI
PubMed
高影响因子
MAIN GROUP CHEMISTRY
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2022
·Vol. 21
·期 1
·315-328
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IF 0.641
Jiang Wen,Zhou Xiaohua,Ni Ke
Soxhlet extraction method was used to extract imperatorin from Angelica dahurica, and the extraction ratio under different extraction condition was optimized to attain the best condition. Then, XAD-16 macroporous resin was selected as the optimal resin to boost the extraction ratio of imperatorin. Afterwards, the higher purity of imperatorin (96.84±0.2%) was separated by preparative HPLC system. Next, the photocatalytic reaction between the above imperatorin and collagen which the highest levels in skin was investigated using UV-vis spectroscopy, amino acid analysis and HPLC analysis. The results showed that imperatorin reacted with collagen only under ultraviolet light which caused the denaturation of collagen, and three new products were generated. The ultraviolet products were isolated by preparative HPLC system and separately detected by high-resolution mass spectrum. The possible UV photocatalytic reaction mechanism between imperatorin and collagen is that ultraviolet light induces the increase of the activity of the imperatorin to react with the tyrosine in the collagen, resulted in the denaturation of collagen and reestablish of the normal epidermal tissue in skin.
研究领域:
药理学
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生物化学
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产品关联
DOI
高影响因子
Journal of Natural Medicines
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2024
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IF 2.5
Gao Chong,Hu Zhong-He,Cui Zhen-Yu,Jiang Yu-Chen,Dou Jia-Yi,Li Zhao-Xu,Lian Li-Hua,Nan Ji-Xing,Wu Yan-Ling
Angelica dahurica (A. dahurica) has a wide range of pharmacological effects, including analgesic, anti-inflammatory and hepatoprotective effects. In this study, we investigated the effect of A. dahurica extract (AD) and its effective component bergapten (BG) on hepatic fibrosis and potential mechanisms. Hepatic fibrosis was induced by intraperitoneal injection with carbon tetrachloride (CCl 4 ) for 1 week, and mice were administrated with AD or BG by gavage for 1 week before CCl 4 injection. Hepatic stellate cells (HSCs) were stimulated by transforming growth factor-β (TGF-β) and cultured with AD, BG, GW4064 (FXR agonist) or Guggulsterone (FXR inhibitor). In CCl 4 -induced mice, AD significantly decreased serum aminotransferase, reduced excess accumulation of extracellular matrix (ECM), inhibited caspase-1 and IL-1β, and increased FXR expressions. In activated HSCs, AD suppressed the expressions of α-SMA, collagen I, and TIMP-1/MMP-13 ratio and inflammatory factors, functioning as FXR agonist. In CCl 4 -induced mice, BG significantly improved serum transaminase and histopathological changes, reduced ECM excessive deposition, inflammatory response, and activated FXR expression. BG increased FXR expression and inhibited α-SMA and IL-1β expressions in activated HSCs, functioning as GW4064. FXR deficiency significantly attenuated the decreasing effect of BG on α-SMA and IL-1β expressions in LX-2 cells. In conclusion, AD could regulate hepatic fibrosis by regulating ECM excessive deposition and inflammation. Activating FXR signaling by BG might be the potential mechanism of AD against hepatic fibrosis.
研究领域:
药理学
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肝病学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2025
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IF 4
Qian Li,Yan-Qiong Luo,Han Zhang,Yu-Jiao Hou,Aleeza Jamil,Wen-jing Li,Bo Hong,Chun-Jie Zhao
Coumarin is an important natural compound in Angelica dahurica that has high value in food, cosmetics, and medicine. In this study, a multitemplate magnetic molecular-imprinted polymer (MMIP) was synthesized via surface imprinting using Fe 3 O 4 @SiO 2 -C=C nanoparticles as carriers and imperatorin, isoimperatorin and oxypeucedanin as templates. The products were characterized by FT-IR spectroscopy, BET adsorption, SEM, TGA, VSM, and XRD. The adsorption performance was evaluated by dynamic, static, and selective adsorption and repeated experiments. The results showed that the MMIP was superior to the magnetic non-imprinted polymer (MNIP) in terms of adsorption capacity and morphological characteristics and was highly reusable. This MMIP can be reused 6 times. Under optimal conditions, the three coumarins of Angelica dahurica were efficiently separated and purified, with an adsorption capacity of 98.18 mg/g and a spiked recovery rate of 96.97-98.69%, and the purity could reach 87-96%. The linear ranges of the methods for imperatorin, isoimperatorin, and oxypeucedanin are 0.0625-1.00 mg/mL, respectively, showing a good correlation (R 2 > 0.9984). Compared with traditional extraction methods, this method has excellent adsorption and extraction performance in the selective separation and purification of three coumarins and provides a new strategy for the efficient extraction of coumarins from Angelica dahurica even other matrix.
研究领域:
药学
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分析化学
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高分子科学
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天然产物提取
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材料科学
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产品关联
DOI
PubMed
高影响因子
REDOX REPORT
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2026
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IF 6.9
Xue-qin He,Yi-zhang Li,Li-ba Xu,Chun-feng Wu,Du-dong Wei,Hao-yu Liu,Fen Qiu
Background Oxidative stress-induced liver injury can progress to severe hepatic conditions. Polysaccharides are promising therapeutic agents, but the protective effects of Angelica dahurica polysaccharide (ADP) against this injury remain unclear.Purpose This study was conducted to elucidate ADP's protective effects and underlying molecular mechanisms in Ovariectomize/D-galactose (OVX/D-Gal) rats.Methods Fifty female OVX rats and ten sham-operated (SO) rats were randomly divided into six groups. Except SO group, rats were administered the corresponding doses of ADP and resveratrol respectively, and the model was established by 49-day daily subcutaneous D-Gal injection. Serum and liver samples were collected to examine the hepatic histopathological changes, oxidative stress-related biomarkers, inflammatory factors, and protein and gene expression levels.Results ADP reduced serum ALT and AST levels (P < 0.01) and improved liver histology. ADP increased SOD and GSH-Px activities , decreased MDA levels (P < 0.05). ADP also lowered pro-inflammatory cytokines levels, upregulated Nrf2, HO-1, and NQO-1 expression, promoted Bcl-2 and suppressed Bax and cleaved caspase-3 (P < 0.05).Conclusions ADP alleviated OVX/D-Gal-induced oxidative stress and liver injury by increasing antioxidant capacity, inhibiting apoptosis, attenuating inflammation, and activating Nrf2/HO-1/NQO-1 signaling pathway.
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DOI
PubMed
高影响因子
PHYTOTHERAPY RESEARCH
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2025
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IF 6.3
Jingyi Hu,Feng Xu,Lei Zhu,Yuan Cui,Ryan Au,Yanan Li,Yiheng Tong,Hong Shen
Angelica dahurica radix (ADR), the root of the botanical family Apiaceae (genus Angelica , species Angelica dahurica ( Hoffm .)), has been used to treat colitis in clinical practice. The immunomodulatory effects of ADR are attributed to its polysaccharides (RP). However, its mechanism of action has not been elucidated. In this study, RP's structure was determined through nuclear magnetic resonance analysis. Dextran sulfate sodium-induced colitis in mice was utilized to assess the therapeutic efficacy of RP, while experiments involving fecal microbiota transplantation (FMT) and antibiotic treatment were performed to investigate the contribution of gut microbiota to RP's protective function. Non-targeted metabolomics was utilized to identify potential targets for elucidating the underlying mechanisms. RP is likely composed of (→4)-α-D-Glcp-(1→ and →4)-α-D-Galp-(1→). It effectively alleviated DSS-induced colitis by restoring the balance of the gut microbial community, a finding validated through FMT and antibiotic intervention experiments. Imidazole propionate (ImP) emerged as a potential target for RP's efficacy in treating colitis, which inhibits the activation of peroxisome proliferator-activated receptor gamma (PPAR-γ). Our findings suggest that RP may confer protection against colitis by activating the PPAR-γ signaling pathway through alleviating the constraint imposed by ImP.
研究领域:
药理学
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微生物学
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免疫学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
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2026
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IF 1.6
Fen Qiu,Xue-Qin He,Yi-Zhang Li,Chun-Feng Wu,Du-Dong Wei,Hao-Yu Liu,Li-Ba Xu
We established in vivo models of hippocampal oxidative injury to investigate the protective effect of Angelica dahurica polysaccharide (ADP) on the hippocampus. ADP alleviated ovariectomized/D-Galactose (OVX/D-Gal)-induced hippocampal damage and enhanced antioxidant capacity (increased SOD/GSH-Px, decreased MDA). Additionally, ADP activated the Nrf2 pathway (upregulated Nrf2, downregulated NQO1/HO-1) and inhibited apoptosis (upregulated Bcl-2, downregulated Bax/cleaved caspase-3). Untargeted metabolomics revealed that ADP reversed seven oxidative stress-related metabolites, and pathway analysis implicated glycerophospholipid metabolism in its antioxidant mechanism. These results demonstrated that ADP protects against OVX/D-Gal-induced hippocampal oxidative injury by activating Nrf2, enhancing antioxidant capacity, inhibiting apoptosis, and regulating glycerophospholipid metabolism.
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DOI
PubMed
高影响因子
Journal of Oleo Science
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2022
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IF 1.628
Chunlian Li,Qiuyang Cai,Xianyi Wu,Zekai Tan,Lewen Yao,Shiyuan Huang,Weicheng Zhang,Zhengyi Hong,Zhuoyu Chen,Lanyue Zhang
Umbelliferae plants, which are widely used as traditional Chinese medicine because of their characteristics of relieving rheumatism, alleviating fever, circulating blood and easing pain. This experimental study was based on ear edema model caused by 12-O-tetracycline-propylphenol-13-acetic acid (TPA) in mice and compared with the Ibuprofen (Ib) group. Gas chromatography-mass spectrometry (GC-MS) was used to analyse the composition of the essential oils from the four studied Umbelliferae plants (Angelica sinensis (Oliv.) Diels, A. dahurica (Hoffm.) Benth. & Hook.f. ex Franch. & Sav., A. pubescens Maxim and Foeniculum vulgare Mill.). Biologically active components in volatile oils from the four studied Umbelliferae plants were evaluated. The expression levels of inflammatory cytokines Tumor Necrosis Factor-α (TNF-α), Cyclooxygenase-2 (COX-2), Interleukin-6 (IL-6) and RelA (p65) in mouse skin were determined by immunohistochemical method. The refractive index of the four essential oils was calculated. A total of 239 compounds were identified by GC-MS from the four studied plants, and the main constituents were osthole (44.61%, APEOs), obepin (0.59%, APEOs & 86.58%, FVEOs), undecanol (8.58%, ADEOs), α-muurolene (7.95%, ADEOs) and cis-anethol (9.11%, ADEOs). E-ligustilide (0.14%, APEOs & 81.14%, ASEOs), (-)-spathulenol (0.08%, FVEOs & 1.21%, ASEOs), (-)-terpinen-4-ol (4.91%, FVEOs), 2-butylthiolane (5.76%, APEOs) and α-bisabolol (3.80%, APEOs). This study showed that all the essential oils from the four studied Umbelliferae plants contained various lactones, including ligustrongolactone, trans-anisol and imperatorin. According to the results of the TPA induction test in the mouse ear edema model, the essential oils of four Umbelliferae plants reduced the levels of inflammatory cytokines TNF-α, COX-2, IL-6 and p65. All of them showed extraordinary biological activity in anti-inflammatory, so they have potential application value for biomedical products, pharmaceutical preparations, natural functional nutrients and cosmetic additives.
研究领域:
药理学
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药物化学
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中医药学
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免疫学
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DOI
PubMed
高影响因子
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