Polymers
·
2019
·Vol. 11
·期 2
·302
·
IF 3.164
Boxiang Wang,Song Zhang,Yifan Wang,Bo Si,Dehong Cheng,Li Liu,Yanhua Lu
At present,Antheraea pernyisilk fibroin (ASF) has attracted research efforts to investigate it as a raw material for fabrication of biomedical devices because of its superior cytocompatibility. Nevertheless, native ASF is not easily processed into a hydrogel without any crosslinking agent, and a single hydrogel shows poor mechanical properties. In this paper, a series of ASF/poly (N-isopropylacrylamide) (PNIPAAm) composite hydrogels with different ASF contents were manufactured by a simple in situ polymerization method without any crosslinking agent. Meanwhile, the structures, morphologies and thermal properties of composite hydrogels were investigated by XRD, FTIR, SEM, DSC and TGA, respectively. The results indicate that the secondary structure of silk in the composite hydrogel can be controlled by changing the ASF content and the thermal stability of composite hydrogels is enhanced with an increase in crystalline structure. The composite hydrogels showed similar lower critical solution temperatures (LCST) at about 32 °C, which matched well with the LCST of PNIPAAm. Finally, the obtained thermosensitive composite hydrogels exhibited enhanced mechanical properties, which can be tuned by varying the content of ASF. This strategy to prepare an ASF-based responsive composite hydrogel with enhanced mechanical properties represents a valuable route for developing the fields of ASF, and, furthermore, their attractive applications can meet the needs of different biomaterial fields.Keywords:Antheraea pernyi;silk fibroin;hydrogel;thermosensitive;mechanical properties
研究领域:
生物材料学
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高分子化学
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组织工程学
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产品关联
DOI
PubMed
高影响因子
Gels
·
2022
·Vol. 8
·期 7
·398
·
IF 4.432
Jia Li,Bo-Xiang Wang,De-Hong Cheng,Yan-Hua Lu
The Antheraea Pernyi silk gland protein originates from natural organisms and synthesized by tussah silk glands and has widely potential biomaterial applications due to the superior biocompatibility. This study investigates the Antheraea Pernyi silk gland protein-based drug-loaded bio-hydrogels for bioengineered tissue fabricated by using an eco-friendly method without the harsh extracting process and the usage of toxic chemicals. The drug-loaded bio-hydrogels exhibited a porous structure and interconnected pore walls. The swelling ratio and water absorption of drug-loaded bio-hydrogels were, respectively, above 95% and 1.5 × 103%. The cumulative release of drug loaded hydrogels all reached more than 90% within 4 h, and this indicates the potential of drug-loaded hydrogels as future drug-carrying biomaterials. RSC96 Schwann cells cultured on drug-loaded hydrogels for 72 h under cell culture medium show no toxic effects and more pro-proliferative effects. The results suggest the suitability of drug-loaded bio-hydrogels as natural biopolymer for the potential in vitro RSC96 cell culture platform and other biomaterial applications.
研究领域:
生物材料
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组织工程
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药物递送系统
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细胞生物学
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DOI
高影响因子
POLYMER ENGINEERING AND SCIENCE
·
2016
·Vol. 57
·期 2
·206-213
·
IF 1.719
Xiufang Li,Qiang Zhang,Dezhan Ye,Jie Zhang,Yuhang Guo,Renchuan You,Shuqin Yan,Mingzhong Li,Jing Qu
To engineer tissue restoration, it is necessary to provide a bioactive, mechanically robust scaffold. Electrospun poly(ε-caprolactone) (PCL) nanofiber is a promising biomaterial candidate with excellent mechanical properties, but PCL scaffolds are inert and lack natural cell recognition sites. To overcome this problem we investigated the incorporation of Antheraea pernyi silk fibroin (ASF) containing inherent RGD tripeptides with PCL in electrospinning process. The mixing ratios showed remarkable impact on the properties of hybrid nanofibers. Increasing PCL content significantly enhanced the mechanical properties of nanofibers. In particular, the mechanical properties were remarkably enhanced when PCL content increased from 50 wt% to 70 wt%. Moreover, the biological assays based on endothelial cells showed promoted cell viability when ASF content reached to 30 wt%. The data demonstrated that the nanofiber containing 70% of PCL and 30% of ASF achieved the most balanced performances for integrating the mechanical properties of PCL and the bioactivity of ASF. Furthermore, biomimetic alignment of 70PCL/30ASF nanofibers was achieved, which could support PC12 neuron-like cell growth and guide neurite outgrowth, providing a potentially useful option for the engineering of oriented tissues. The results show that the PCL/ASF hybrid nanofibers can be considered as a promising candidate for tissue engineering scaffolds. POLYM. ENG. SCI., 57:206–213, 2017. © 2016 Society of Plastics Engineers
研究领域:
生物材料
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组织工程
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生物医学工程
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材料科学
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DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 13
·2282
·
IF 5.1
Yu Liu,Ruixin Liu,Onanong Phuseerit,Hua Li,Sirithon Siriamornpun
To enhance the nutritional and functional properties of triticale noodles, this study assessed their proximate composition, sensory attributes, and antioxidant activity, along with protein and starch digestibilities when supplemented with varying concentrations (0%, 5%, 10%, 15%) of silkworm (Antheraea pernyiandBombyx mori) pupa powder (SP). Incorporating SP into triticale noodles led to significant enhancements in protein, fat, and ash contents (p< 0.05). The addition of SP may also lead to noticeable color and texture differences by decreasing the lightness (L*) and increasing hardness and springiness in noodles, as well as the flavor difference revealed by E-nose and E-tongue analysis. Furthermore, the total phenolic content and abilities to scavenge DPPH and ABTS radicals improved as the SP level increased. Compared to the control, the SP-fortified noodles had a significantly high in vitro protein digestibility but a low estimated glycemic index. Overall, due to their enhanced nutritional value and bioactivities, SP triticale noodles could be regarded as a healthier alternative to traditional noodles.
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 45
·28401-28409
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IF 3.361
Boxiang Wang,Hangdan Xu,Jia Li,Dehong Cheng,Yanhua Lu,Li Liu
At present, Antheraea pernyi silk fibroin (ASF) based hydrogels have wide potential applications as biomaterials because of their superior cytocompatibility. Herein, ASF is used as a nucleophilic reagent, reacted with allyl glycidyl ether (AGE) for the preparation of allyl silk fibroin (ASF-AGE). The investigation of ASF-AGE structure by 1H NMR and FTIR are revealed that reactive allyl groups were obtained on ASF by nucleophilic substitution. A series of ASF based hydrogels are manufactured by N-isopropylacrylamide (NIPAAm) copolymerization bridged with ASF-AGE. By the silk fibroin self-assembly process, stably physical cross-linked hydrogels are formed without any crosslinking agent. These hydrogels exhibit good thermoresponsive and degradability, for which the LCST was about 32 °C, and these hydrogels can be degraded in protease XIV solution. Excellent cell proliferation, viability and morphology is demonstrated for b End.3 cells on the hydrogels by the characteristic MTT assay, CLSM and SEM. The cytocompatibility of b End.3 cells was demonstrated with excellent cell adhesion and growth on these ASF based hydrogels in vitro. These degradable and thermoresponsive ASF based hydrogels may find potential applications for cells delivery devices and tissue engineering.
研究领域:
生物材料学
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组织工程学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Polymers
·
2021
·Vol. 14
·期 1
·108
·
IF 4.329
Bo-Xiang Wang,Jia Li,De-Hong Cheng,Yan-Hua Lu,Li Liu
Antheraea pernyisilk fibroin (ASF)-based nanofibers have wide potential for biomaterial applications due to superior biocompatibility. It is not clear whether the ASF-based nanofibers scaffold can be used as an in vitro cancer cell culture platform. In the current study, we fabricated novel ASF-based thermoresponsive hydrogel nanofibers by aqueous electrospinning for colon cancer (LoVo) cells culture. ASF was reacted with allyl glycidyl ether (AGE) for the preparation of allyl silk fibroin (ASF-AGE), which provided the possibility of copolymerization with allyl monomer. The investigation of ASF-AGE structure by1H NMR revealed that reactive allyl groups were successfully linked with ASF. ASF-based thermoresponsive hydrogel nanofibers (p (ASF-AGE-NIPAAm)) were successfully manufactured by aqueous electrospinning with the polymerization of ASF and N-isopropylacrylamide (NIPAAm). The p (ASF-AGE-NIPAAm) spinning solution showed good spinnability with the increase of polymerization time, and uniform nanofibers were formed at the polymerization time of 360 min. The obtained hydrogel nanofibers exhibited good thermoresponsive that the LCST was similar with PNIPAAm at about 32 °C, and good degradability in protease XIV PBS solution. In addition, the cytocompatibility of colon cancer (LoVo) cells cultured in hydrogel nanofibers was assessed. It was demonstrated that LoVo cells grown on hydrogel nanofibers showed improved cell adhesion, proliferation, and viability than those on hydrogel. The results suggest that the p (ASF-AGE-NIPAAm) hydrogel nanofibers have potential application in LoVo cells culture in vitro. This study demonstrates the feasibility of fabricating ASF-based nanofibers to culture LoVo cancer cells that can potentially be used as an in vitro cancer cell culture platform.Keywords:Antheraea pernyisilk fibroin;nanofibers;thermoresponsive;cancer cell;in vitro culture
研究领域:
生物材料学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Proteomics
·
2019
·
IF 3.537
Ruru Chen,Cheng Zhu,Mingzhou Hu,Lian Zhou,Hui Yang,Hailing Zheng,Yang Zhou,Zhiwen Hu,Zhiqin Peng,Bing Wang
Achieving efficient identification of silk protein requires highly sensitive analytical techniques and favorable extraction methods , which is of great significance to the research of ancient silk, especially for the controversial issue of the silk origin. In this paper, proteomics and western blot were proposed to analyze the silk proteins of Bombyx mori ( B. mori ) and Antheraea pernyi ( A. pernyi ) dissolved by different methods. First, the differences in secondary structure were detected via spectroscopy . LC-MS/MS was then employed to characterize the peptides of silk proteins precisely. LiBr solution exhibited outstanding dissolution effect on B. mori cocoon, with 87 proteins detected; while copper-ethylenediamine solution (CED) was more appropriate for A. pernyi cocoon, and 16 proteins were identified in A. pernyi -CED. In addition to fibroin and sericin , abundant seroins, enzymes , protease inhibitors , other functional proteins and uncharacterized proteins were detected. Based on the LC-MS/MS data, diagnostic antibodies for the two species were prepared, and fibroin was successfully identified by western blot assay because both dissolution methods were gentle and did not destroy the antigenic epitopes in the protein molecule. Owing to their good specificity and high sensitivity, these diagnostic antibodies have good application prospects in immunoassays of different silk species. Significance This study presents the comprehensive analysis on silk identification of proteins from B. mori and A. pernyi extracted by different methods via the proteomic and immunology as well as the conventional approaches. Great coverage of two cocoon proteomes was accomplished, which demonstrated the outstanding difference in components and abundance. Based on the proteomics analysis, the diagnostic antibodies against two species were prepared and identified the corresponding fibroin successfully in the completed protein mixtures. To our knowledge, the proteomic and immunology procedures with high efficiency, sensitivity and specificity are novel analysis on the silk identification and has great potential in the field of ancient silk detection.
研究领域:
蛋白质组学
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分析化学
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免疫学
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考古科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 22
·3956
·
IF 5.1
Yu Liu,Yangran Lu,Poompatchara Nakkote,Hua Li,Ruixin Liu,Sirithon Siriamornpun
The study intended to assess the impacts of partially replacing wheat flour withAcheta domesticuspowder (AD) andAntheraea pernyipupae powder (AP) at 5%, 10%, and 15% levels on the proximate composition, color properties, texture profile, antioxidant capacity, starch digestibility, and flavor characteristics of Chinese steamed bread (CSB). The addition of AP and AD notably increased the levels of protein, fat, and ash and also led to an elevated chewiness and hardness. Most importantly, compared to the control, AP- and AD-fortified CSB exhibited a significantly low estimated glycemic index (eGI) and high total phenolic and flavonoid contents, resulting in improved DPPH and ABTS radical scavenging activities. Furthermore, E-nose, E-tongue, and GC-MS analysis revealed that incorporation of AP and AD strengthened umami and saltiness and enriched the flavor profile of CSB. Our findings indicate that AD- and AP-fortified CSB is a promising functional food with a lower eGI, as well as improved nutritional value and antioxidant potential, offering a sustainable strategy for staple food innovation while also providing guidance for consumers to select wheat flour fortified with different types and levels of insect powder to prepare CSB based on their preferences.
研究领域:
食品科学与技术
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营养生物化学
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功能食品
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可持续食物来源
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创新主食
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产品关联
DOI
PubMed
高影响因子
Gels
·
2022
·Vol. 9
·期 1
·4
·
IF 4.432
Jia Li,Bo-Xiang Wang,De-Hong Cheng,Yue Zhang,Qiang Yao,Xin-bin Ji,Jing Guo,Yan-Hua Lu
To improve the versatility of the sodium alginate-loaded bio-hydrogels, Antheraea pernyi silk gland protein/sodium alginate drug-loaded hydrogels were prepared by using an eco-friendly multiple network cross-link technology. Fourier transform infrared (FT-IR) spectroscopy and UV-Vis spectrophotometer were used separately to evaluate the chemical structure and drug release behavior of drug-loaded hydrogels. The antibacterial drug carrier gels were evaluated by using inhibition zone test against theS. aureusandE. coli. The CCK-8 assay was used to assess the biocompatibility of drug loaded hydrogels. The FT-IR results showed that there was a strong interaction within the drug loaded hydrogels, and the ASGP was beneficial to enhance the interaction within the drug loaded hydrogels. UV-Vis spectrophotometer results indicated the cumulative release reached 80% within 400 min. Antibacterial bio-hydrogels had a good antibacterial property, especially the antibacterial bio-hydrogels with bacitracin exhibits superior to other antibacterial agents. The drug-loaded bio-hydrogels exhibited the adhesion and proliferation of RSC96 cells and perfected biocompatibility. This provides a new idea for further research and development of tissue-friendly drug-loaded biomaterials.Keywords:antheraea pernyi silk gland protein;sodium alginate;multiple network hydrogel;tissue engineering;drug release systems
研究领域:
材料科学
·
生物医学工程
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2021
·Vol. 10
·期 8
·2001695
·
IF 9.933
Yajun Shuai,Huan Lu,Ruyin Lv,Jie Wang,Quan Wan,Chuanbin Mao,Mingying Yang
The use of biomacromolecules as templates to control the nucleation and growth of hydroxyapatite crystals to prepare bioactive materials is a valuable approach in bone tissue engineering. Here, an artificial periosteum is prepared by biomineralizing Antheraea pernyi fibroin ( AF ) membrane with prenucleated nanoclusters, which can promote the osteogenic differentiation of mesenchymal stem cells (MSCs) and induce the formation of bone matrix protein in vivo. To achieve this, a biologically inspired prenucleated calcium and phosphorus nanocluster mineralization system is designed to nucleate and generate hydroxyapatite crystals on the surface of the AF membrane. This biomineralization process provides AF membranes with improved elastic modulus and tensile strength. Subsequently, cell viability assay, hemolysis test, and H&E staining show that the mineralized AF ( MAF ) membranes has good cytocompatibility, hemocompatibility, and histocompatibility in vitro and in vivo. Additionally, the MAF membranes significantly promote osteogenic differentiation of MSCs in the absence of osteogenic inducer in vitro. Experiments in vivo demonstrate that bone-related matrix proteins are highly expressed in MAF groups with or without MSCs seeded. Therefore, the use of bioinspired prenucleated nanoclusters to prepare artificial periosteum based on biomineralized AF membrane is a promising strategy in the field of bone tissue engineering.
研究领域:
骨组织工程
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生物材料
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生物医学工程
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材料科学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）