INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2025
·Vol. 26
·期 10
·4496
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IF 4.9
Chunyuhang Xu,Siyu Chen,Tiange Liu,Haowen Zhu,Chien-Liang Kuo,Zhuoyu Zhou,Guo Chen,Fion Wei Lin Chin,Xin Yang,Dejian Huang
Antrodia cinnamomea is widely known for its bioactive properties, particularly in anti-cancer, anti-inflammatory, and antibacterial areas. Despite the full use of the bioactive compounds from its fruiting body, high-value residues remain largely underexploited. This study presents a novel one-pot gel formation method, utilizing cinnamomea cellulose-riched residues to create hydrogels as an effective wound-healing dressing. The hydrogels derived from these residues show desirable properties, including non-drying characteristics, antibacterial activity against Staphylococcus aureus ATCC 1768, and cytocompatibility. Residual bioactive compounds, such as Antcin-K, Dehydroeburicoic acid, and (25S,R)-Antcin H, were identified in the residues, adding to the hydrogel’s efficacy. A UVB irradiation model was employed to evaluate the protective effects of the residues on UVB-damaged HaCaT skin cell lines, with an IC 50 of 0.045 mg/mL. The results indicated that A. cinnamomea residue extracts reduced the upregulation of MMP-1, MMP-2, MMP-3, MMP-7, and MMP-9 proteins caused by UVB exposure, suggesting high UV-protective activity. Additionally, antibacterial tests on Staphylococcus aureus strains, including Staphylococcus ATTC 1768, showed promising results, with inhibition zones ranging from 10.64 to 12.11 mm. In summary, Antrodia cinnamomea residue hydrogels combine UV protection with antimicrobial activity, making them a promising candidate for medical applications, particularly as a wound-healing dressing.
研究领域:
生物材料学
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药理学
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医学工程
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微生物学
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PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Zhi-Qiang Liu,Chuan-Zhi Yan,Shu-Mei Zhong,Chao-Jie Chong,Ya-Qi Wu,Jun-Yang Liu,Chun-Xiang Huang,Ke-Ying Wang,He-Wei Li,Jia-Le Song
Polycystic ovary syndrome (PCOS) is a prevalent endocrine and metabolic disorder. This study investigated the mitigating effects of the Antrodia cinnamomea polysaccharide (ACP) on a letrozole-induced PCOS rat model. Results demonstrated that ACP reduced obesity and ameliorated dyslipidemia in PCOS rats. Moreover, ACP restored estrous cycle regularity, suppressed polycystic ovarian changes, and regulated serum levels of sex hormones, SOD, and MDA. Furthermore, ACP increased the α-diversity and modulated the abundance of phyla (Bacteroidetes, Firmicutes, and Verrucomicrobia) and genera (Lactobacillus, Helicobacter, Akkermansia, Oscillospira, Coprococcus, Roseburia, Blautia, and Allobaculum) in the gut microbiota. ACP also restored compromised intestinal barriers by upregulating the expression of ZO1, Occludin, Claudin1, and Claudin7 in the colon. ACP mitigated ovarian fibrosis by preventing activation of the NLRP3 inflammasome, decreasing the expression of fibrotic markers (TGF-β1, collagen-I, α-SMA, and CTGF), and regulating four ovarian fibrosis-associated metabolomics pathways. Generally, dietary ACP effectively ameliorated clinical symptoms and inhibited ovarian fibrosis in PCOS rats.
研究领域:
医学
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生物学
·
营养学
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PubMed
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Jia-Hui Fan,Lin-Qiang Xiong,Wei Huang,Jia-Qi Hong,Huang-Kai Guo,Ka-Hing Wong,Peter C.K. Cheung,Qiong-Qiong Yang,Bo-Bo Zhang
Microparticle-enhanced cultivation was used to enhance the production of exopolysaccharides (EPSs) from Antrodia cinnamomea . The structure and antibacterial activity of two EPSs produced by A. cinnamomea treated with Al 2 O 3 [EPS-Al (crude) and EPS-Al-p (purified)] and without Al 2 O 3 [EPS-C (crude) and EPS-C-p (purified)] were compared. It was observed that the addition of 4 g/L Al 2 O 3 at 0 h resulted in the highest EPS yield of 1.46 g/L, possible attributed to the enhanced permeability of the cell membrane. The structural analysis revealed that EPS-C-p and EPS-Al-p had different structures. EPS-C-p was hyperbranched and spherical with a Mw of 10.8 kDa, while EPS-Al-p was irregular and linear with a Mw of 12.5 kDa. The proportion of Man in EPS-Al-p decreased, while those of Gal and Glc increased when compared to EPS-C-p. The total molar ratios of 6-Glc p and 4-Glc p in EPS-Al-p are 1.45 times that of EPS-C-p. Moreover, EPSs could alter bacterial cell morphology, causing intracellular substance leakage and growth inhibition, with EPS-Al having a stronger antibacterial activity than EPS-C. In conclusion, A. cinnamomea treated with Al 2 O 3 could produce more EPSs, changing monosaccharide composition and glycosidic linkage profile, which could exert stronger antibacterial activity than that produced by untreated A. cinnamomea .
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高影响因子
Fermentation-Basel
·
2023
·Vol. 9
·期 2
·185
·
IF 3.7
Yingying Li,Jiaxin Ge,Yanbin Li,Siqi Zheng,Yangyang Liu,Yunxiang Liang,Yuxia Mei
Antrodia cinnamomea, a rare medicinal fungus endemic to Taiwan, contains numerous active components and displays strong antitumor and anti-inflammatory effects. We isolated and purified a novelA. cinnamomeaactive protein (termed ACAP) from liquid fermentation mycelia and evaluated its antitumor activity. A homogeneous protein-eluted fraction was obtained by anion exchange chromatography and gel filtration chromatography, and ACAP was identified based on the antitumor activity screening of this fraction. An in vitro assay of three tumor cell lines (HeLa, Hep G2, and Hepa 1-6) revealed significant antiproliferative effects of ACAP at low concentrations, with IC50values of 13.10, 10.70, and 18.69 µg/mL, respectively. Flow cytometric analysis showed that ACAP induced late apoptosis of Hep G2 cells. The apoptosis rate of 50 µg/mL ACAP-treated cells (60%) was significantly (p< 0.01) more than that of the control. A Western blotting assay of apoptotic pathway proteins showed that ACAP significantly upregulated p53 and downregulated caspase-3 expression levels. Our findings indicate that ACAP has strong antitumor activity and the potential for development as a therapeutic agent and/or functional food.Keywords:Antrodia cinnamomeaactive protein;antitumor activity;isolation and purification;apoptosis
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Food & Function
·
2018
·Vol. 9
·期 12
·6517-6525
·
IF 3.289
Yutian Zhang,Diying Li,Zhuo Wang,Wanting Zang,Pan Rao,Yunxiang Liang,Yuxia Mei
To enhance production of Antrodia cinnamomea triterpenoids (ACTs) from mycelia in solid-state culture, α-terpineol was added to the medium as an elicitor at an optimal concentration of 0.05 mL L−1. Multi-stage solvent extraction and HPLC analysis were performed, and the compositions of ACTs-E (from culture with elicitor) and ACTs-NE (from culture without elicitor) were found to be quite different. In assays of in vitro antitumor activity, ACTs-E, in comparison with ACTs-NE, produced stronger viability reduction in several tumor cell lines and stronger apoptosis induction in HeLa in a dose-dependent manner. Several related proteins involved in the mitochondrial pathway of apoptosis (p53, Bax, caspase-3) did not show expression upregulation by ACTs-E, suggesting that apoptosis induction occurred through a p53-independent process. Further analysis revealed that ACTs-E strongly inhibited synthesis of topoisomerase I (TOP1) and tyrosyl-DNA phosphodiesterase I (TDP1), which are involved in DNA repair, at both transcriptional and protein levels. Our findings suggest that ACTs-E have potential for applications in the pharmaceutical, clinical, and functional food industries, as a novel antitumor agent and a dual TOP1/TDP1 inhibitor.
研究领域:
药理学
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真菌学
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肿瘤学
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PubMed
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Fermentation-Basel
·
2023
·Vol. 9
·期 10
·880
·
IF 3.7
Siqi Zheng,Mingyue Fang,Jiaxin Huang,Yanbin Li,Yuxia Mei
Antrodia cinnamomea(AC), a rare fungus endemic to Taiwan, contains high levels of various secondary metabolites, notably triterpenoids, having useful medicinal and pharmacological properties. Techniques for increasing the production of AC triterpenoids (ACT) for medicinal purposes are a high research priority. We measured and compared the biomass and ACT content of AC mycelia under various liquid fermentation culture conditions. Relative gene expression levels of ten enzymes involved in the mevalonate (MVA) pathway and “subsequent group modification pathway” were determined, and correlation analysis was performed to evaluate the roles of these enzyme genes in ACT synthesis. Two representative genes encoding the enzymes lanosterol synthase (AcLSS) and sterol C-24 reductase (AcERG4), whose activity is closely associated with ACT content, were selected for homologous expression.AcLSSandAcERG4were separately linked to plasmid pCT74, and transformed into prepared AC protoplasts to obtain two recombinant strains, termed RpLSS and RpERG4, by polyethylene glycol (PEG)-CaCl2-mediated protoplast transformation. Upregulated expression levels ofAcLSSandAcERG4(1.78- and 1.41-fold, respectively) were associated with significantly higher (1.82- and 1.37-fold, respectively) ACT content in the recombinant strains in comparison with the wild-type. Our findings provide a theoretical and practical basis for the enhancement of ACT production using homologous expression techniques.Keywords:Antrodia cinnamomea;triterpenoids;homologous expression;synthetic pathway
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