CANADIAN ENTOMOLOGIST
·
2025
·
IF 1.1
Lina Guo,Yu Zhang,Jue Wang,Diandian Yu,Min Wu,Yuan Guo
Temperature is the most significant abiotic factor that affects the growth and behaviour of insects. However, the mechanism by which the olfactory system senses thermal stimulus and combines temperature and chemical signals to trigger certain behavioural outputs is unclear. This study aimed to clarify the mechanism by which environmental temperature affects olfactory perception in Apis cerana cerana (Hymenoptera: Apidae). We used quantitative reverse-transcriptase polymerase chain reaction and western blotting to analyse the expression of AcerOr1 and AcerOr2. We also used electroantennography (EAG) assays to detect bee antennal responses to odorants at different temperatures. The results revealed that the mRNA expression of AcerOr1 and AcerOr2 was significantly influenced by temperature. These genes exhibited both increases and decreases in expression over time, with the most significant differential observed at 25 °C. Protein expression was similarly affected at 2 hours after different temperature treatments. Electroantennography responses from the antennae revealed that six odorant volatiles – N-(4-ethylphenyl)-2-((4-ethyl-5-(3-pyridinyl)-4H-1,2,4-triazol-3-yl)thio)acetamide (VUAA1), linolenic acid, eugenol, hexyl acetate, 1-nonanol, and lauroleic acid – had the most dramatic effect at 25 °C. The results indicate that environmental factors affecting the expression of AcerOr1 and AcerOr2 modulate olfactory recognition behaviour in A. cerana cerana, suggesting that changes in environmental temperature can affect bees’ olfactory preferences.
研究领域:
昆虫学
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分子生物学
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DOI
高影响因子
Toxics
·
2024
·Vol. 12
·期 2
·131
·
IF 3.9
Qingxin Meng,Rong Huang,Shunhua Yang,Hui Li,Dan Yue,Xueyang Gong,Wenzheng Zhao,Yakai Tian,Kun Dong
Honey bees play a critical role as pollinators. However, their reproduction success and survival face severe threats due to the deterioration of their living environment. Notably, environmental conditions during their preimaginal stage inside brood cells can influence their immune capabilities and overall health after emergence. During the in-cell developmental stage, workers are in close contact with cocoons, which can become a source of stress due to accumulated metals. To investigate this potential threat, experiments were conducted to examine the impact of cocoons in brood cells used to rear different generations on the metal content and detoxification gene expression levels inApis cerana cerana. Our findings indicated significant differences in the layers, weight, base thickness, and metal contents like Cr, Cd, Pb, Mn, Ni, and As of cocoons in multi-generation brood cells compared to single-generation brood cells. These increases led to significant elevations in metal levels and upregulations of the four CYP450 detoxification genes in both six-day-old larvae and newly emerged workers. In conclusion, this study highlights the negative impact of cocoons in multi-generation brood cells on bee health and provides evidence supporting the development of rational apiculture management strategies for ecosystem stability.
研究领域:
昆虫学
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环境科学
·
分子生物学
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Jiao Wu,Yun Duan,Zhaoyin Gao,Xinfeng Yang,Dongxiang Zhao,Jinglin Gao,Wensu Han,Guosheng Li,Shijie Wang
The physicochemical parameters, sugars, minerals and volatile compounds were selected in current work as the crucial parameters for evaluating multifloral honeys produced by Apis cerana cerana Fabricius, Apis dorsata Fabricius and Lepidotrigona flavibasis Cockerell. A summarized physicochemical profile of multifloral honeys exhibited great variability, which was quite different from that of A. mellifera honey, regarding color (83.86–328.36 mmPfund), ash (0.30–0.34 g 100 g −1 ), soluble solid (74.72–80.41 °Brix), diastase activity (5.48–6.70 °Göthe), electrical conductivity (0.40–0.55 mS cm −1 ), free acidity (0.51–0.85 mol kg −1 ), hydroxymethylfurfural (0–39.28 mg kg −1 ), moisture (18.65–23.80%) and pH (3.73–3.95). Fructose and glucose were the major carbohydrates in Apis spp. honeys but high level of maltose was present in L. flavibasis honey. The potassium , zinc and calcium were abundant elements in multifloral honeys. The principal component analysis was applied to discriminate honeys from different bee species by cobalt, sulfur, magnesium, color, maltose, pH, free acidity, potassium, molybdenum, F/G, HMF and iron, and it was concluded that L. flavibasis honey had better quality compared to the other honeys. Moreover, the specific volatile compounds detected in A. dorsata and L. flavibasis honeys were more than A. cerana cerana honey, which could be used as the characteristic markers discriminating honey samples.
研究领域:
农业
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食品科学
·
化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Jiao Wu,Xiang-Tan Xu,Cheng Xing,Xin-Bao Hao,Xing-Yue Fang,Zhi-Hao Xie,Shan Zhao,Jing-Lin Gao,Lu Xu,Shi-Jie Wang
Honey has been recognized for its role in disease prevention through nutritional modulation. In this study, we comprehensively assessed the physicochemical parameters, metabolic profile, antioxidant and anti-inflammatory activities of multifloral honeys produced by Apis cerana cerana in Sansha City (SS), Hainan Province. Metabolomic analysis identified SS honey had multiple bioactive compounds known for antioxidant and anti-inflammatory properties. Compared to A. cerana cerana honey from central regions of Hainan Province, SS honey showed higher levels of TFC (113.80 mg RE/100 g) and TPC (45.86 mg GAE/100 g). Furthermore, SS honey demonstrated greater antioxidant activity, as evidenced by FRAP (1503.16 μmol TE/kg), DPPH IC 50 (36.13 mg/mL), and ABTS IC 50 (11.03 mg/mL). SS honey significantly reduced the levels of cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS), and tumor necrosis factor-α (TNF-α) in lipopolysaccharide (LPS)-induced RAW 264.7 cells, along with suppressing the mRNA expression of these inflammatory markers.
研究领域:
养蜂学
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生物化学
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药理学
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营养科学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
Insects
·
2026
·Vol. 17
·期 8
·769
·
IF 3
Lina Guo,Yu Zhang,Chang Song,Yuan Guo
Simple SummaryThe Hedgehog signaling pathway is highly conserved across species, yet its role in insect olfaction remains poorly understood. Here, we characterized the Smoothened (Smo) gene—a core component of this pathway—in the eastern honey beeApis cerana cerana (A. c. cerana). We found thatAcerSmoexhibits a unique spatial segregation pattern: its mRNA is transcribed in sensory neurons beneath antennal trichoid and placoid sensilla, while the protein localizes to adjacent non-neuronal supporting cells, revealing a spatial separation betweenAcerSmomRNA and protein localization, suggesting cell-type-specific regulation ofAcerSmoexpression during olfactory function. RNA interference-mediated silencing ofAcerSmoupregulated the expression of key olfactory receptor genesAcerOr1andAcerOr2, and altered bees’ electrophysiological and behavioral responses to ecologically relevant odorants including 2-heptanone, 1-nonanol, and eugenol. Our findings demonstrate thatAcerSmomodulates honey bee olfactory recognition by regulating olfactory receptor expression, expanding the known physiological functions of the Hedgehog pathway beyond development into adult sensory regulation.Smoothened (Smo) is a core component of the Hedgehog signaling pathway and plays an important role in insect tissue growth, but its function in honey bees remains unclear. In this study, we investigated the expression localization ofAcerSmoin the antennae ofApis cerana cerana (A. c. cerana)and its impact on olfactory behavior using in situ hybridization, immunohistochemistry, RNA interference, transcriptome sequencing, and behavioral and electrophysiological techniques. The results showed thatAcerSmomRNA is expressed in the sensory neurons beneath trichoid and placoid sensilla, while the protein is localized to the non-neuronal supporting cells of these sensilla, revealing a spatial separation betweenAcerSmomRNA and protein localization. Although this pattern suggests cell-type-specific post-transcriptional regulation, the present data do not directly demonstrate intercellular mRNA transport or translation. RNAi-mediated silencing ofAcerSmosignificantly upregulated the expression ofAcerOr1andAcerOr2. Transcriptome sequencing identified 19 olfaction-related differentially expressed genes involved in signal transduction, substance transport, and various metabolic pathways. Electroantennogram and Y-tube olfactometer assays revealed that afterAcerSmointerference, the responses ofA. c. ceranato odorants changed significantly: EAG responses to 2-heptanone and hexyl acetate decreased, while avoidance behavior toward 2-heptanone increased; EAG responses to 1-nonanol and eugenol increased, and Y-tube tests showed enhanced attraction to 1-nonanol and increased avoidance of eugenol. Conclusions: These findings indicate thatAcerSmoplays an important role in regulating olfactory receptor expression and modulating odorant responses in honey bees, thereby influencing their olfactory behavior.
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产品关联
DOI
高影响因子
JOURNAL OF EXPERIMENTAL BIOLOGY
·
2025
·Vol. 228
·期 18
·
IF 2.6
Chen Longlong,Gong Yu,Ma Weihua,Jiang Yusuo,Zhao Huiting
The olfactory senses of Apis cerana cerana are crucial to their essential activities. This study aimed to investigate the effect of phased starvation, insulin injections and insulin silencing on the olfactory abilities of A. cerana cerana . A starvation regression curve was established; it was found that the mortality of bees increased significantly after 9 h of starvation. Measurement of insulin levels in the bees revealed a gradual decrease after 3 and 6 h of starvation, followed by a sudden rise at 9 h. Additionally, starvation induced changes in the olfactory responses of bees: starvation decreased the electroantennogram values of antennae for most odorants, changed behavioral preferences for some floral odorants and affected the expression of olfactory genes. High insulin levels enhanced electroantennogram response values of the antennae, changing most odorant selections from attraction or neutrality to avoidance. In contrast, low insulin levels decreased the electroantennogram response values of the antennae and predominantly shifted odor preferences toward attraction. These two insulin conditions resulted in opposite results, and changes in olfactory gene expression also exhibited differing trends. It was found that low-insulin conditions caused by starvation for 3 h and 6 h as well as insulin silencing exhibited similarities in multiple olfactory metrics. This suggests that starvation-induced olfactory changes may be attributed to the reduction of endogenous insulin levels. The study results reveal a strong correlation between insulin and olfaction in A. cerana cerana , providing a foundation for future investigations into the underlying regulating mechanisms of insulin/insulin-like signaling on olfaction. These findings also offer research directions for enhancing bee pollination capacity and regulating other olfactory behaviors.
研究领域:
昆虫生理学
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神经生物学
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分子内分泌学
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化感生物学
·
行为生态学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Jiao Wu,Bingjun Han,Shan Zhao,Yihai Zhong,Wensu Han,Jinglin Gao,Shijie Wang
Honey is a complex food product, and has been linked to a number of substantial health advantages. The aim of this study was to assess the phenolic compounds, organic acids, microbial community, antioxidant activity, and antimicrobial activity of multifloral honeys produced by Apis cerana cerana , A. dorsata , and Lepidotrigona flavibasis in Hainan province, China. Our results illustrated that chlorogenic acid was the major phenolic component in all honeys. L. flavibasis honey showed the greatest antioxidant activity (DPPH IC 50 value, 7.66 mg/mL; FRAP value, 5217.06 µmol TE/kg), as well as the highest levels of proline (548.64 mg/kg), total flavonoid (11.67 mg QE/100 g), total phenolic (132.73 mg GAE/100 g), and Lactobacillus (96.28 %). Besides, all honeys were found to have a broad spectrum of antibacterial activity. Overall, our data imply that Hainan honeys, particularly stingless bee honeys, have been beneficial components of human diets.
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DOI
PubMed
高影响因子
Insect Science
·
2024
·
IF 2.9
Huiting Zhao,Longlong Chen,Miaomiao Liu,Shuguo Zhao,Weihua Ma,Yusuo Jiang
Insulin receptors (InR) are an integral component of the insulin/insulin-like growth factor signaling pathway, which plays a vital role in insect development, lifespan, reproduction, and olfactory sensitivity. However, whether InR participate in the peripheral olfactory system of insects remains unclear. Recently, we found that 2-heptanone (2-HT) affects AcerInR expression, the gene for an InR protein, in Apis cerana cerana . We then examined the spatiotemporal expression profile of the gene in A. cerana cerana . The mRNA of AcerInR was primarily expressed in the antennae, wings, and legs of forager bees, which are probable chemosensory tissues. The results of fluorescence competitive binding assays, combined with site-directed mutagenesis, demonstrated that AcerOBP6 and AcerOBP14 exhibit strong binding affinities to 2-HT. Furthermore, after foragers were fed with double-stranded AcerInR , the expression levels of AcerOBP6 and AcerOBP14 decreased significantly, as did the electroantennogram responsiveness to 2-HT and some other odorants. In conclusion, our findings provide a foundation for understanding the involvement of AcerInR in the odor perception of A. cerana cerana . Moreover, they offer novel insights into the olfactory recognition mechanism in insects.
研究领域:
昆虫学
·
分子生物学
·
神经生物学
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产品关联
DOI
PubMed
高影响因子
Insects
·
2025
·Vol. 16
·期 9
·961
·
IF 2.9
Huiman Zhang,Lele Sun,Peng Wang,Jiaoxin Xie,Yuan Guo
Simple SummaryAs a native species in China; the survival and reproduction ofApis cerana ceranaare heavily dependent on chemical signals. In the process of chemical communication, the antennae of bees serve as the primary sensing organs, and the legs also play an important role in the reception and transmission of chemical signals. In our study, scanning electron microscopy (SEM) was used to observe its legs, revealing two types of sensilla, and the ultrastructure of the tibial spur and the antennal brush was observed. The electrophysiological responses of the legs (ELG), along with Y-tube behavioral assays, were employed to validate the involvement of the bee’s legs in olfactory perception. The legs of bees at different ages showed varying degrees of significant response to nonanal and ocimene. Notably, 10-day-old bees exhibited a clear preference for nonanal and ocimene. These findings contribute to a deeper understanding of the mechanistic role of the honeybee leg in chemical signal detection, elucidating their functional characteristics in chemical communication, and providing experimental evidence for the study of bee behavioral responses and environmental adaptation.Apis cerana ceranais a key social insect, and its ability to recognize chemical signals is crucial for maintaining colony homeostasis and coordinating collective behaviors, such as foraging, nursing, and defense. The legs of insects play a significant role in gustatory perception and proximity olfactory perception. In this study, the leg sensilla ofA. c. ceranawere observed by scanning electron microscopy (SEM). Two types of sensilla were observed, including sensilla trichodea (Str I, Str II, Str III, Str IV, Str V, and Str VI) and sensilla chaetica (Sch I, Sch II, and Sch III). The two unique structures of the tibial spur (Tsp I, Tsp II) and antennal brush (Abr) are carefully observed. The electrophysiological responses of workers at different ages to diverse chemical compounds were measured via electrolegogram (ELG) recordings on their legs. The results showed that 1-day-oldA. c. ceranawas more sensitive to nonanal; 10-day-old and 25-day-oldA. c. ceranawere more sensitive to ocimene. The results of behavioral responses showed that nonanal and ocimene can significantly attract 10-day-old workers ofA. c. cerana. This study establishes a foundation for further exploration of the mechanisms by which the legs ofA. c. ceranafacilitate colony-level communication through chemical signals. It also provides an important theoretical basis for understanding their social organization and information transmission.
研究领域:
昆虫学
·
神经行为学
·
化学生态学
·
感觉生物学
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产品关联
DOI
PubMed
高影响因子
FOOD SCIENCE AND BIOTECHNOLOGY
·
2023
·
IF 2.9
Wu Jiao,Zhao Shan,Chen Xin,Jiu Yuanda,Liu Junfeng,Gao Jinglin,Wang Shijie
Honey quality is in relation to botanical origin, and physicochemical properties, elemental composition, and antioxidant activity have been used for assessment and identification of honeys. The goal of this study is to contribute to the general analysis of five unifloral honeys from Cocos nucifera L., Dalbergia benthami Prain, Bombax ceiba L., Castanea mollissima Bl., and mangrove in Hainan province, China. Our results revealed that B. ceiba honey had the highest pH (4.27), color (139.33 mm Pfund), ash content (1.03 g/100 g), and electrical conductivity (1312.00 μS/cm) in five unifloral honeys. Furthermore, B. ceiba honey also contained the highest levels of total phenolic content (75.54 mg GAE/100 g) and total flavonoid content (29.22 mg RE/100 g), as well as the strongest antioxidant activity (DPPH IC 50 value, 3.97 mg/mL; FRAP value, 6527.43 µmol TE/kg). Moreover, we revealed a considerable variation in element contents in honeys using ICP-MS, with potassium being the most predominant element. B. ceiba honey had the highest contents of K, Ca, Mg, and P, whereas the highest amount of Na was found in mangrove honey. Overall, our data indicated that B. ceiba honey deserves further research as a potential antioxidant agent.
研究领域:
农业
·
食品科学
·
分析化学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Sheng-nan Li,Hao Wu,Rui-quan Hou,Jing-tong Zhou,Su-kun Lin,Kun-yu Zhao,Rui-fei Wang,Su-qing Huang,Zhi-xiang Zhang
O -Carboxymethyl chitosan nanoparticles ( O -CMC-NPs), which are organic pesticide carriers, have excellent application potential. Exploring the effects of O -CMC-NPs on non-target organisms, such as Apis cerana cerana , is critical for their effective application; however, such studies are limited. This study investigated the stress response of A. cerana Fabricius after O -CMC-NPs ingestion. The administration of high O -CMC-NP concentrations enhanced the activities of antioxidant and detoxifying enzymes in A. cerana , with the activity of glutathione- S -transferase increasing by 54.43 %–64.33 % after one day. The transit of O -CMC-NPs into the A. cerana midgut resulted in their deposition and adherence to the intestinal wall, as they cluster and precipitate in acidic conditions. The population of Gillianella bacteria in the middle intestine was remarkably reduced after 6 d of administration of high O -CMC-NP concentrations. Contrastingly, the abundance of Bifidobacteria and Lactobacillus in the rectum significantly increased. These results indicate that the intake of high concentrations of O -CMC-NPs causes a stress response in A. cerana and affects the relative abundance of crucial intestinal flora, which may pose a potential risk to the colony. This implies that even nanomaterials with favorable biocompatibility should be applied reasonably within a specific range to avoid adverse effects on the environment and non-target organisms in the context of large-scale research and promotion of nanomaterials.
研究领域:
纳米技术
·
昆虫学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2022
·
IF 4.966
Hongyan Peng,Dezheng Guo,Wenlu Shan,Zhenguo Liu,Hongfang Wang,Lanting Ma,Baohua Xu,Xingqi Guo
The cyclin-dependent kinase (CDK) protein family plays an important role in regulating life functions, such as the cell cycle and metabolism. This study reports the first cloning and functional analysis of A. cerana cerana CDK1 (AccCDK1) . The distribution profile of AccCDK1 in different developmental periods and different tissues was determined. The experimental results showed that the distribution of AccCDK1 was tissue-specific. AccCDK1 distribution at the transcriptional and translational levels was affected by stress conditions induced by H 2 O 2 , UV, HgCl 2 , CdCl 2 , extreme temperatures (4 °C, 44 °C) and pesticides (avermectin, lambda-cyhalothrin, haloxyfop- R -methyl, and glyphosate), which resulted in changes in the expression levels. These results suggest that AccCDK1 may have an important part to play in honey bee resistance to stress. The expression of a recombinant AccCDK1 protein in vitro enhanced the antistress capacities of E. coli and yeast, which suggests that AccCDK1 is related to the stress response. When AccCDK1 was silenced, the expression of some antioxidant genes was downregulated, and the enzymatic potencies of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were reduced, which suggests that AccCDK1 takes part in the body's resistance to oxidative stress upon external stimulation by influencing relevant antioxidants. Notably, the survival rate of A. cerana cerana under high-temperature-induced stress decreased after AccCDK1 silencing, which verifies our results. In conclusion, we found that AccCDK1 played an indispensable function in resisting oxidative stress and maintaining normal cellular functions.
研究领域:
分子生物学
·
昆虫学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）