JOURNAL OF CELLULAR PHYSIOLOGY
·
2021
·Vol. 236
·期 8
·5921-5936
·
IF 6.384
Sijia Liang,Jing Luo,Muna Alariqi,Zhongping Xu,Aoli Wang,Muhammad Naeem Zafar,Jun Ren,Fuqiu Wang,Xuefei Liu,Yanfeng Xin,Haonan Xu,Weifeng Guo,Yanqin Wang,Weihua Ma,Lizhen Chen,Keith Lindsey,Xianlong Zhang,Shuangxia Jin
Plant bugs (Miridae species) have become major agricultural pests that cause increasing and severe economic damage. Plant-mediated RNA interference (RNAi) is emerging as an eco-friendly, efficient, and reliable strategy for pest management. In this study, we isolated and characterized a lethal gene of Apolygus lucorum and named it Apolygus lucorum LIM ( AlLIM ), which produced A. lucorum mortality rates ranging from 38% to 81%. Downregulation of the AlLIM gene expression in A. lucorum by injection of a double-stranded RNA (dsRNA) led to muscle structural disorganization that resulted in metamorphosis deficiency and increased mortality. Then we constructed a plant expression vector that enabled transgenic cotton to highly and stably express dsRNA of AlLIM ( dsAlLIM ) by Agrobacterium -mediated genetic transformation. In the field bioassay, dsAlLIM transgenic cotton was protected from A. lucorum damage with high efficiency, with almost no detectable yield loss. Therefore, our study successfully provides a promising genetically modified strategy to overpower A. lucorum attack.
研究领域:
植物保护
·
分子生物学
·
农业科学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Physiology
·
2022
·
IF 4.755
Yin Haichen,Li Wenjing,Xu Min,Xu Dong,Wan Peng
The mirid bugs Apolygus lucorum and Adelphocoris suturalis are considered serious pests of many crops in China, the host plant recognition of these pests remains unclear. The current study investigated the vital odor cues of two mirid bugs and evaluated the role of olfactory recognition in host recognition. The GC-EAD response of mirid bugs to volatiles of their host plant Phaseolus vulgaris was tested. Tetradecane, 2-propyl-1-pentanol, and dodecanal elicited strong EAG responses by mirid bugs and were tested with field experiments. The results indicated tetradecane was significantly more attractive than other attractants, yielding 30.33 ± 2.19 mirid bugs trapped during 7 days. The selected response rates to tetradecane were above 60%, which was most attractive to female A. lucorum at 1.5 mg/ml. Among seven tetradecane derivatives, tetradecane and tetradecanoic acid were the most potent attractants to A. lucorum and A. suturalis. Tetradecane was present in the volatiles of 10 common hosts, and their difference in relative content was significant. The presence of tetradecane seemed relevant to the olfactory response intensity of two mirid bugs towards the different host plants. The artificial supplement of tetradecane increased the attractive effect of host plants. These results suggested that tetradecane plays a vital role in the olfactory selection by two mirid bugs, and it can be made into field baits as a novel ecological strategy to manage these pests with widely reported pesticide resistance. However, results suggested host recognition is not entirely dependent on odor cues. We demonstrated that A. suturalis and A. lucorum adults have similar olfactory recognition mechanisms to their hosts in long-distance host selection. While, the differences in host plant selection between the two pests should occur in close range due to differences in gustatory or tactile sensory organs of A. lucorum and A. suturalis.
研究领域:
昆虫学
·
生态学
·
农业科学
✓ 已验证
DOI
高影响因子
JOURNAL OF INSECT SCIENCE
·
2015
·Vol. 15
·期 1
·
IF 1.025
Shi Jizhe,Liu Xinchao,Li Zhen,Zheng Yuanyuan,Zhang Qingwen,Liu Xiaoxia
The aim of this research was to evaluate acute toxicity of the essential oil of leaves of Chinese chives, Allium tuberosum Rottler ex Spreng (Asparagales: Alliaceae) and its major constituents against Apolygus lucorum Meyer-Dür (Hemiptera: Miridae). The essential oil of A. tuberosum leaves was obtained by hydrodistillation and analyzed by gas chromatography and gas chromatography-mass spectrometry. The major constituents of the oil were sulfur-containing compounds, including allyl methyl trisulfide (36.24%), diallyl disulfide (27.26%), diallyl trisulfide (18.68%), and dimethyl trisulfide (9.23%). The essential oil of A. tuberosum leaves exhibited acute toxicity against Ap. lucorum with an LD 50 value of 20.03 μg per adult. Among the main compounds, diallyl trisulfide (LD 50 = 10.13 μg per adult) showed stronger acute toxicity than allyl methyl trisulfide (LD 50 = 21.10 μg per adult) and dimethyl trisulfide (LD 50 = 21.65 μg per adult). The LD 50 value of diallyl disulfide against Ap. lucorum was 28.10 μg per adult. The results indicated that the essential oil of A. tuberosum and its major constituents may have a potential to be developed as botanical insecticides against Ap. lucorum.
研究领域:
植物学
·
昆虫学
·
农药科学
✓ 已验证
DOI
高影响因子
Insects
·
2021
·Vol. 12
·期 10
·870
·
IF 2.769
Han Chen,Honghua Su,Shuai Zhang,Tianxing Jing,Zhe Liu,Yizhong Yang
Simple SummarySince the widespread adoption of Bt cotton in the late 1990s, the green mirid bug,Apolygus lucorum(Hemiptera: Miridae), has become one of the most important pests in cotton fields and some other crops. To manage this destructive pest,Peristenus spretus(Hymenoptera: Braconidae) has been tested in augmentative biological control. In this study, after cotton plants were damaged by different densities ofA. lucorum, the behavioral responses ofA. lucorumandP. spretusto cotton plants volatiles were evaluated, and the quality and quantity of volatiles from cotton plants were analyzed. The results demonstrated that HIPVs emitted by plants in response toA. lucorumcould be influenced by the pest density and could be identified byP. spretusas a signal of the host. Our results would help understand howP. spretusplays a role in biological control againstA. lucorum.AbstractPlants would release herbivore-induced plant volatiles (HIPVs) to repel herbivores and attract natural enemies after being damaged by herbivores. In this study, after cotton plants were damaged by different densities ofApolygus lucorum, the behavioral responses ofA. lucorumandPeristenus spretusto cotton plants volatiles were evaluated, and the quality and quantity of volatiles from cotton plants were analyzed. Only when cotton plants were damaged by four bugs did bothA. lucorumandP. spretusshow an obvious response to damaged cotton plants, which indicates that cotton defense is correlated with pest density. The collection and analysis of volatiles reveals that the increase in pest density results in the emission of new compounds and an increase in the total number of volatiles with an alteration in proportions among the compounds in the blend. These changes in volatile profiles might provide wasps and mirids with specific information on host habitat quality and thus could explain the behavioral responses of parasitoids and pests.Keywords:behavior;pest;natural enemy;HIPVs;density
研究领域:
昆虫学
·
植物保护
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）