JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
·
2017
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IF 3.454
Qilai Zhou,Yasuhisa Oya,Takumi Chikada,Wen Zhang,Lihong Xue,Youwei Yan
Ultrafine lithium titanate (Li 2 TiO 3 ) powder was synthesized by hydrothermal method . The phase formation and transition condition among α, β, and γ-Li 2 TiO 3 were discussed. XRD and ICP-AES showed the single α-phase was formed at 180 °C with 2 h hydrothermal reaction, and it transited into β-phase at 400 °C. SEM observation and EDS analysis confirmed the dissolution of TiO 2 and the formation of α-Li 2 TiO 3 proceeded simultaneously with preferable growth direction of (-133) lattice. During the phase transition, the powder maintained the small crystallite , which facilitated the fabrication of Li 2 TiO 3 bulk with small grain size. After the Ar + irradiation, the surface region to the depth of 3 μm of Li 2 TiO 3 ceramic was affected, where the decrease of crystallization and disturbance of short-range order were confirmed by GIXRD and Raman spectroscopy . In spite of the structure change at the surface area, the ceramic bulk maintained the same.
研究领域:
材料科学
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核能工程
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产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2021
·
IF 3.935
Xin-Nan Wang,Wen-Rui Xia,Jian-Qun Liu,Fang-Yuan Sun,Zhu-Jun Zhong,Li-Fang Liu,Gui-Zhong Xin
Liver toxicity induced by Triptolide (TP) has limited its clinical application on rheumatoid arthritis (RA). Saponins have been proved as an efficacious remedy to mitigate hepatotoxicity. However, the mechanism of reducing hepatotoxicity by saponins intervention remains incompletely characterized. Tryptophan (Trp) metabolites activate transcriptional regulators to mediate host detoxification responses. Our study aimed to investigate whether Clematichinenoside AR (C-AR) could attenuate TP-induced liver damage by regulating Trp metabolism. We used targeted metabolomics to quantify Trp metabolites in the serum and liver samples of collagen-induced arthritis rats treated by TP. Multiple comparison analyses helped the evaluation of promising biomarkers. The pronounced changed levels of Trp, indole acetic acid, and indole-3-carboxaldehyde in the serum and indole acetic acid , indole , and tryptamine in the liver are relevant to TP-induced liver injury. Intervention with C-AR could relieve TP-induced hepatotoxicity evidenced by ameliorative serum parameters and hepatic histology. In addition, C-AR regulated the levels of these indoles biomarker candidates to normal. Therapeutic modulation with natural compounds might be a useful clinical strategy to ameliorate toxicity induced by TP. Deciphering Trp metabolism will facilitate a better understanding of the pathogenesis of diseases and drug responding.
研究领域:
药理学
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代谢组学
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毒理学
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免疫学
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DOI
高影响因子
APOPTOSIS
·
2024
·Vol. 29
·期 9
·1679-1695
·
IF 6.1
Fan Aoyu,Li Yunpeng,Zhang Yunyan,Meng Wei,Pan Wei,Chen Meixi,Ma Zhongliang,Chen Wei
Prostate cancer (PCa) is one of the most common cancers affecting the health of men worldwide. Castration-resistant prostate cancer (CRPC), the advanced and refractory phase of prostate cancer, has multiple mechanisms of resistance to androgen deprivation therapy (ADT) such as AR mutations, aberrant androgen synthase, and abnormal expression of AR-related genes. Based on the research of the AR pathway, new drugs for the treatment of CRPC have been developed in clinical practice, such as Abiraterone and enzalutamide. However, many areas in this pathway are still worth exploring. In this study, single-cell sequencing analysis was utilized to scrutinize significant genes in the androgen receptor (AR) pathway related to CRPC. Our analysis of single-cell sequencing combined with bulk-cell sequencing revealed a substantial downregulation of AR-regulated AFF3 in CRPC. Overexpression of AFF3 restricted the proliferation and migration of prostate cancer cells whilst also increasing their sensitivity towards enzalutamide, while knockdown of AFF3 had the opposite effect. To elucidate the mechanism of tumor inhibition by AFF3, we applied GSVA and GSEA to investigate the metabolic pathways related to AFF3 and revealed that AFF3 had an impact on fatty acids metabolism and ferroptosis through the regulation of ACSL4 protein expression. Based on correlation analysis and flow cytometry, we can speculate that AFF3 can impact the sensitivity of the CRPC cell lines to the ferroptosis inducer (RSL3) by regulating ACSL4. Therefore, our findings may provide new insights into the mechanisms of drug resistance in CRPC, and AFF3 may serve as a novel prognostic biomarker in prostate cancer.
研究领域:
肿瘤学
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分子生物学
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基因组学
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DOI
高影响因子
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
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2020
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IF 1.646
Yan Jin Gao,Wei Jie Fu,Jia Liu,Yi Juan Chen,Guang Hui Dai
Cucumber powdery mildew (PM) is a destructive fungal disease which has a substantial impact on cucumber yield and quality. Our previous work has demonstrated that (+)-( S )-ar-turmerone (ATM) is an effective compound for controlling PM infected by Podosphaera xanthii in cucumber. In this study, the effect of ATM on cucumber PM was histologically investigated by observations on the morphology of the pathogenic fungus at different time points after inoculation. It was found that the ATM caused the collapse of the pathogen's conidia and mycelia structure, the cytoplasm coagulation, the reduction of the conidia germination rate, and the inhibition of the conidiophore growth, therefore, prevented the re-infection of the P. xanthii . Evaluation of the content of total phenols and flavonoids in cucumber ATM-treated leaves showed that the ATM-treatment could increase the content of total phenols and flavonoids in cucumber leaves significantly in comparison to the control. A further analysis of the contents of the compounds showed that ATM-treatment could increase the content of rutin , quercetin , hesperetin , and gallic acid , syringic acid , vanillic acid and salicylic acid. Meanwhile, the activity of phenylalanine ammonia-lyase (PAL) and polyphenol oxidase (PPO) in cucumber leaves were increased slightly, but the activity of peroxidase (POD) and superoxide dismutase (SOD) were significantly increased through ATM treatment. It should be estimated from this study that the ATM not only directly affected the growth of P. xanthii but also enhanced the cucumber plant resistance to the disease.
研究领域:
植物病理学
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植物化学
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DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2024
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IF 5.3
Ran Chen,Junyu Zou,Zihao Mao,Zhongwei An,Pei Chen,Xinbing Chen
Laser display technology can enhance the image quality of liquid–crystal on silicon (LCoS) devices in AR displays. High birefringence (Δ n ), large dielectric anisotropy (Δ ε ), low rotational viscosity ( γ 1 ) and good blue-light stability are inevitable performance requirements for achieving fast response time, low voltage and 2π phase modulation in LCoS devices based on laser display technology. However, people lack attention to how to obtain liquid crystal compounds with above properties. In this work, we propose to utilize one fluorinated terphenyl unit with excellent blue-light stability as the molecular skeleton, and then employ large polar terminal groups to modulate the molecular polarizability and dipole moment , and finally screen to obtain the LCs with high Δ n and large Δ ε . The effects of methyl, fluorinated, chlorinated and cyano terminal groups on the mesomorphic and electro-optical properties of fluorinated terphenyl LCs were systematically studied. Experimental and theoretical results show that the target compounds exhibit wide LC phase intervals, and could enhance the Δ n , Δ ε of the parent LC mixture and reduce its viscoelastic constant, further meaning that they would help to improve the electro-optical properties of LC mixtures for AR displays. Our results would provide experimental data and technical support for the future development of LC mixtures with high blue-light stability for AR displays.
研究领域:
材料科学
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光学
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显示技术
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DOI
高影响因子
Current Protocols
·
2024
·Vol. 4
·期 4
·e1033
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IF 0
Ruifeng Wang,Shuhua Ma,Nengwei Xu,Yumiao Gan,Pengya Li,Jingying Zhang,Zhixiang Zhang,Qingyang Gu,Jian Xiang
Prostate cancer is a leading diagnosis and major cause of cancer-related deaths in men worldwide. As a typical hormone-responsive disease, prostate cancer is commonly managed with androgen deprivation therapy (ADT) to curb its progression and potential metastasis. Unfortunately, progression to castration-resistant prostate cancer (CRPC), a notably more aggressive phase of the disease, occurs within a timeframe of 2-3 years following ADT. Enzalutamide, a recognized androgen receptor (AR) antagonist, has been employed as a standard of care for men with metastatic castration-resistant prostate cancer (mCRPC) since it was first approved in 2012, due to its ability to prolong survival. However, scientific evidence suggests that sustained treatment with AR antagonists may induce acquired AR mutations or splice variants, such as AR F877L, T878A, and H875Y, leading to drug resistance and thereby diminishing the therapeutic efficacy of these agents. Thus, the establishment of prostate cancer models incorporating these particular mutations is essential for developing new therapeutic strategies to overcome such resistance and evaluate the efficacy of next-generation AR-targeting drugs. We have developed a CRISPR (clustered regularly interspaced short palindromic repeats)-based knock-in technology to introduce an additional F877L mutation in AR into the human prostate cell line LNCaP. This article provides comprehensive descriptions of the methodologies for cellular gene editing and establishment of an in vivo model. Using these methods, we successfully identified an enzalutamide-resistant phenotype in both in vitro and in vivo models. We also assessed the efficacy of target protein degraders (TPDs), such as ARV-110 and ARV-667, in both models, and the corresponding validation data are also included here. © 2024 Wiley Periodicals LLC. Basic Protocol 1 : Generation of AR F877L-mutated LNCaP cell line using CRISPR technology Basic Protocol 2 : Validation of drug resistance in AR F877L-mutated LNCaP cell line using the 2D CTG assay Support Protocol : Testing of sgRNA efficiency in HEK 293 cells Basic Protocol 3 : Validation of drug resistance in AR F877L-mutated LNCaP cell line in vivo
研究领域:
肿瘤学
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分子生物学
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基因编辑
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药理学
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DOI
SEPARATION AND PURIFICATION TECHNOLOGY
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2019
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IF 5.107
Sharafat Ali,Izaz Ali Shah,Haiou Huang
The need for high-performance and novel technologies for selective treatment of radionuclides in water has recently stimulated broad research. In this study, we examined the ability of carbon nanotube (CNT) membranes in selective removal of strontium (Sr 2+ ) and cesium (Cs + ) from water and wastewater. In single-metal systems that contained only Sr 2+ , Cs + or their competitive metal ions, the distribution coefficients ( K d ) of functionalized CNT membranes for divalent cations, i.e., Sr 2+ , Ca 2+ , and Mg 2+ , were measured as 4.41 mmol g −1 , 2.14 mmol g −1 , and 1.30 mmol g −1 , respectively, and those for monovalent cations, i.e., Cs + , K + and Na + , were 0.81 mmol g −1 , 0.79 mmol g −1 , and 0.81 mmol g −1 , respectively. When Cs + or Sr 2+ co-existed with a competing cation, both pristine and functionalized CNT membranes showed excellent selectivity to Sr 2+ over Mg 2+ and Ca 2+ while their selectivity to Cs + over K + and Na + diminished. Moreover, the CNT membranes remained their selectivity for Sr 2+ in a wastewater effluent despite the complex water composition. Overall, the selectivity sequence of the CNT membranes for divalent cations support the occurrence of inner-sphere complexation on CNT surfaces, while that for the monovalent cations was probably determined by electrostatic interactions. This study demonstrates that Ar/O 2 plasma treatment is a greener technology for the production of CNT functionalized membranes for selective removal of contaminants from water to achieve “fit-for-purpose” treatment.
研究领域:
环境科学
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材料科学
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水处理技术
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产品关联
DOI
高影响因子
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
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2025
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IF 3.3
Xinjing Shi,Qingjing Gao,Wenjing Lu,Yuning Xie,Haolin Zhang,Dong Zhang,Yuning Liu,Qiang Weng
SREBP1 (sterol regulatory element binding protein 1) plays an important role in maintaining lipid balance in mammals. Previous research showed that androgen significantly regulated the secretion of lipid-rich musky odor substances from the scented glands of muskrats. Our investigation provides insights into the pathways through which androgen induces SREBP1 expression and affects seasonal changes in triglyceride synthesis in muskrat scented glands. During the breeding season, pronounced Oil Red O staining was confirmed in the scented glandular tissues, and transmission electron microscopy displayed a marked increase in lipid droplets. The scented glands exhibited a higher triglyceride content, which coincided with augmented circulating testosterone, dihydrotestosterone, as well as increased scented glandular testosterone and dihydrotestosterone concentrations. Transcriptomic, proteomic and metabolomic analyses of the scented glands revealed associations with fatty acid biosynthesis, while plasma metabolomic data indicated an increase in metabolites serving as precursors during the breeding season for de novo fatty acid synthesis. Expression levels of genes and proteins related to lipid synthesis and lipid droplets, as well as FASN activity, were significantly elevated in the breeding season. Immunolocalization studies demonstrated the presence of these proteins in glandular and epithelial cells in both seasons. Moreover, in vitro treatment of the scented glandular cells with testosterone resulted in elevated expressions of triglyceride synthesis-related genes. These findings suggested that androgen modulates triglyceride synthesis to enhance lipid droplet formation via the AR-SREBP1-FASN Axis in the scented glands of muskrats, thereby shedding light on the hormonal regulation of lipid metabolism in seasonal breeders.
研究领域:
分子生物学
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内分泌学
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生物化学
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生殖生物学
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比较生理学
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脂质代谢
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动物生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Plasma Processes and Polymers
·
2023
·Vol. 20
·期 5
·2200228
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IF 3.5
Siyuan Sui,Hongmei Sun,Guohua Ni,Yafeng Liu,Huan Zheng,Tao Sun,Ling Kong,Zhongyang Ma,Fangli Yuan
The surface of polytetrafluoroethylene (PTFE) is activated by argon (Ar), Ar mixed with water vapor (Ar-H 2 O), and a mixture of Ar, water vapor, and ammonia (Ar-H 2 O-NH 3 ) plasmas and then grafted onto polymerized dopamine/ethylene imine and immobilized heparin. Oxygen-containing functional groups are all introduced onto the PTFE surface treated by three types of plasma. The amount of oxygen grafted is the highest in samples treated with Ar-H 2 O-NH 3 plasma compared to that treated with Ar-H 2 O plasma. An increase in the content of oxygen grafted onto PTFE contributes to an increase in the thickness and cross-linking bond of the polymeric interlayer and then raises the density of the heparin coating. The thickness of the polydopamine/polyethylenimide intermediate layer on the surface of PTFE treated with Ar-H 2 O-NH 3 plasma is 4.8 ± 0.3 μm, and the density of the immobilized heparin coating was 95 μg/cm 2 .
研究领域:
材料科学
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表面化学
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生物医学工程
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高分子科学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
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2020
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IF 8.758
Peng Jin,Xiaoying Niu,Fang Zhang,Kui Dong,Hongxia Dai,Huige Zhang,Weifeng Wang,Hongli Chen,Xingguo Chen
Covalent organic frameworks (COFs), as one of the most significant members of the porous organic frameworks, have been well used in the photocatalysis owing to their outspread π-conjugated framework, high crystallinity and regular pore structure. Herein, after reducing the labile imine-linked COF-300 to the more stable amine-linked COF-300-AR, we for the first time demonstrated that COF-300-AR was the light-responsive oxidase mimic. COF-300-AR exhibited excellent oxidase-mimicking activity under purple light stimulation (λ = 400 nm), which can catalyze the oxidation of classical substrates such as 3,3′,5,5′-tetramethylbenzydine (TMB) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) by the formation of •OH and O2•– free radicals in the presence of dissolved oxygen. The COF-300-AR oxidase mimic has outstanding advantages of easy light control, high stability, good reusability, and highly catalytic oxidation capacity and has been applied to detect glutathione (GSH) levels in HL60 cells with good selectivity and high sensitivity. This study will broaden the sensing applications of COFs and offer a promising build block for the construction of artificial enzymes.
研究领域:
材料科学
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纳米技术
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分析化学
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生物化学
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产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2025
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IF 6.1
Biyu Wu,Zirui Cheng,Xiang Li,Minxing Liang,Xue Wang,Duan Pi,Jiayi Liu,Huiling Li,Jun Zhao,Junjie Wang,Fang Liang,Xuegeng Wang
As a major substitute for the bisphenol A (BPA), the use of the bisphenol F (BPF) has increased dramatically in recent years. Growing evidence suggest that BPF shares numerous toxicological properties with BPA, raising the concerns about its potential impact on the health of organisms. However, the developmental toxicity of BPF remains poorly understood. In this study, we conducted a 5-day BPF exposure experiment on zebrafish ( Danio rerio ) from blastula stage at concentrations of 2, 20, 200, and 2000 µg/L. Our results demonstrated a significant increase in hatching rates across all treatment groups at 2 days post-fertilization (dpf). The esr1 was significantly upregulated at 2000 µg/L by 5 dpf, while no significant change was observed in ar . The frequency of operculum loss significantly increased at exposure concentrations of 20, 200, and 2000 µg/L, and a notable increase in notochord loss was observed at 2000 µg/L. To explore the underlying mechanisms, transcriptomic analysis was performed to identify differentially expressed genes (DEGs). GO and KEGG pathway enrichment analysis revealed that the toxic effects of BPF were closely associated with osteoclast differentiation, the FoxO signaling pathway, and the MAPK signaling pathway. These pathways influenced critical biological processes, including response to stimuli, animal organ morphogenesis, detoxification, and biomineralization. This study provides evidence that BPF exposure at environmentally relevant concentrations (2 µg/L) is harmful to hatching, concentrations above 20 µg/L exhibit estrogenic-disrupting activity and exert toxicological effects on the development of the head skeleton in zebrafish. These effects are particularly linked to disruptions in osteoclast differentiation.
研究领域:
环境毒理学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology
·
2021
·Vol. 305
·期 7
·1672-1681
·
IF 2.064
Yongdui Ruan,Yaohua Fan,Yanfeng Xie,Chunling Ma,Bingquan Mo,Yanni Lai,Geng Li,Xiaohong Liu,Weihong Kuang
Effective therapeutics are not available for acute lung injury (ALI) and acute respiratory distress syndrome. Modified Xiaoqinglong decoction (M-XQL) is reported to effectively treat pneumonia, but the underlying mechanisms are unclear. In this study, the therapeutic effect and mechanism of M-XQL were examined using a lipopolysaccharide (LPS)-induced ALI mouse model. The effects of M-XQL on lung injury, inflammatory responses, and cell apoptosis were analyzed. Additionally, high-throughput sequencing was performed to evaluate the therapeutic mechanism of M-XQL. Pretreatment with M-XQL significantly and dose-dependently mitigated the pathological changes and upregulation of pulmonary, nitric oxide content and cell apoptosis and serum tumor necrosis factor-alpha contents in the LPS-induced ALI mouse model. RNA sequencing analysis revealed that the expression of several arachidonic acid metabolism-associated genes in the LPS + high-dose M-XQL group differed from that in the LPS group. In particular, the Cbr2 , Cyp4f18 , and Cyp2e1 levels were upregulated, whereas the Alox12 , Ptges , and Ptges2 levels were downregulated in the LPS + high-dose M-XQL group. These results suggest that M-XQL exerts therapeutic effects in ALI mice by regulating arachidonic acid metabolism and exerting anti-apoptotic and anti-inflammatory effects. Thus, M-XQL is a potential agent for the clinical treatment of ALI.
研究领域:
药理学
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呼吸病学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
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