WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
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2017
·Vol. 33
·期 2
·1-14
·
IF 1.658
Guan Jian,Shen Songdong,Wu Hao,Liu Xin,Shen Weijie,He Yuan,Duan R.
The filamentous Cyanobacterium Arthrospira is commercially produced and is a functional, high-value, health food. We identified 5 low temperature and low light intensity tolerant strains of Arthrospira sp. (GMPA1, GMPA7, GMPB1, GMPC1, and GMPC3) using ethyl methanesulfonate mutagenesis and low temperature screening. The 5 Arthrospira strains grew rapidly below 14 °C, 43.75 μmol photons m −2 s −1 and performed breed conservation at 2.5 °C, 8.75 μmol photons m −2 s −1 . We used morphological identification and molecular genetic analysis to identify GMPA1, GMPA7, GMPB1 and GMPC1 as Arthrospira platensis , while GMPC3 was identified as Arthrospira maxima . Growth at different culture temperatures was determined at regular intervals using dry biomass. At 16 °C and 43.75 μmol photons m −2 s −1 , the maximum dry biomass production and the mean dry biomass productivity of GMPA1, GMPB1, and GMPC1 were 2057 ± 80 mg l −1 , 68.7 ± 2.5 mg l −1 day −1 , 1839 ± 44 mg l −1 , 60.6 ± 1.8 mg l −1 day −1 , and 2113 ± 64 mg l −1 , 77.7 ± 2.5 mg l −1 day −1 respectively. GMPB1 was chosen for additional low temperature tolerance studies and growth temperature preference. In winter, GMPB1 grew well at mean temperatures <10 °C, achieving 3258 mg dry biomass from a starting 68 mg. In summer, GMPB1 grew rapidly at mean temperatures more than 28 °C, achieving 1140 mg l −1 dry biomass from a starting 240 mg. Phytonutrient analysis of GMPB1 showed high levels of C-phycocyanin and carotenoids. Arthrospira metabolism relates to terpenoids, and the methyl- d -erythritol 4-phosphate pathway is the only terpenoid biosynthetic pathway in Cyanobacteria. The 1-deoxy- d -xylulose 5-phosphate reductoisomerase (DXR) gene from GMPB1 was cloned and phylogenetic analysis showed that GMPB1 is closest to the Cyanobacterium Oscillatoria nigro-viridis PCC711. Low temperature tolerant Arthrospira strains could broaden the areas suitable for cultivation, extend the seasonal cultivation time, and lower production costs.
研究领域:
微生物学
·
生物化学
·
遗传学
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DOI
PubMed
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Kefan Ouyang,Qian Chen,Hexiang Xie,Qin Zhang,Liling Tao,Hua Xiong,Yulan Du,Jiangang Yan,Xinliang Mao,Qiang Zhao
Arthrospira cell residue (ACR) is a byproduct of the spirulina industry that can be valorized into protein hydrolysates. In this study, we used alkaline protease to hydrolyze ACR and evaluated the effects of hydrolysis time on the properties and functionalities of the hydrolysates. We measured the degree of hydrolysis (DH), particle size, fluorescence, UV‒visible absorption, surface hydrophobicity , emulsification , solubility, and antioxidant activity of the hydrolysates. The DH reached a maximum of 10.2% ± 0.6% after 240 min. Hydrolysis also altered the structure and conformation of the ACR protein, as shown by changes in fluorescence, UV-visible absorption, and FTIR spectra. Hydrolysis enhanced the solubility, surface hydrophobicity , and emulsifying activity of the hydrolysates but not the emulsion stability. The hydrolysates also exhibited higher antioxidant activity than ACR. These results indicate that enzymatic hydrolysis can convert ACR into valuable protein hydrolysates.
研究领域:
生物技术
·
食品科学
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产品关联
DOI
高影响因子
Chinese Journal of Natural Medicines
·
2026
·Vol. 24
·期 2
·227-236
·
IF 7.5
Rakotomalala Manda Heriniaina,Xia Chen,Yulian Wu,Wenfeng Liao,He Fei,Peipei Wang,Kan Ding
Pancreatic cancer, specifically pancreatic ductal adenocarcinoma (PDAC), ranks among the most prevalent malignancies and is a leading cause of cancer-related mortality worldwide. Therefore, there is an urgent need to identify novel anti-pancreatic cancer agents. This study reports a newly identified homogeneous polysaccharide, designated ESPPW, isolated from Arthrospira platensis ( A. platensis ). The molecular weight of ESPPW is estimated at 356 kDa, and it consists predominantly of glucose and rhamnose, with minor amounts of mannose, glucuronic acid, galacturonic acid, galactose, xylose, arabinose, and fucose. ESPPW inhibits the proliferation and migration of PDAC cells both in vitro and in vivo . Mechanistic investigations reveal that ESPPW induces apoptosis through activation of caspase-3 and is associated with upregulation of the tumor-suppressor protein p53. Notably, treatment with 2.8 nmol·L −1 of ESPPW leads to significant time-dependent downregulation of galectin-3 (Gal-3) and glypican-6 (GPC-6). These findings are corroborated by immunohistochemical analysis of tumor xenograft tissues. Furthermore, overexpression of Gal-3 and GPC-6 reverses the pro-apoptotic effect of ESPPW, as indicated by restored cycle regulatory proteins (CDK2) expression. In conclusion, these data demonstrate that ESPPW suppresses PDAC cell growth by promoting apoptosis and disrupting the functional activity of Gal-3 and GPC-6.
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Chunli Yu,Yao Hu,Yuqin Zhang,Wei Luo,Jing Zhang,Peilun Xu,Jun Qian,Jun Li,Jianfeng Yu,Jin Liu,Wenguang Zhou,Shengxi Shao
In industrial-scale cultivation of microalgae, salinity stress often stimulates high-value metabolites production but decreases biomass yield. In this research, we present an extraordinary response of Arthrospira platensis to salinity stress. Specifically, we observed a significant increase in both biomass production (2.58 g L −1 ) and phycocyanin (PC) content (22.31%), which were enhanced by 1.26-fold and 2.62-fold, respectively, compared to the control, upon exposure to exogenous glycine betaine (GB). The biochemical analysis reveals a significant enhancement in carbonic anhydrase activity and chlorophyll a level, concurrent with reductions in carbohydrate content and reactive oxygen species (ROS) levels. Further, transcriptomic profiling indicates a downregulation of genes associated with the tricarboxylic acid (TCA) cycle and an upregulation of genes linked to nitrogen assimilation, hinting at a rebalanced carbon/nitrogen metabolism favoring PC accumulation. This work thus presents a promising strategy for simultaneous enhancement of biomass production and PC content in A. platensis and expands our understanding of PC biosynthesis and salinity stress responses in A. platensis .
研究领域:
生物技术
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分子生物学
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DOI
PubMed
高影响因子
Marine Drugs
·
2023
·Vol. 21
·期 2
·133
·
IF 5.4
Congling Liu,Gong Chen,Hailian Rao,Xun Xiao,Yidan Chen,Cuiling Wu,Fei Bian,Hailun He
Crude enzymes produced by a marine bacteriumPseudoalteromonassp. JS4-1 were used to hydrolyze phycobiliprotein. Enzymatic productions showed good performance on DPPH radical and hydroxyl radical scavenging activities (45.14 ± 0.43% and 65.11 ± 2.64%, respectively), especially small peptides with MWCO <3 kDa. Small peptides were fractioned to four fractions using size-exclusion chromatography and the second fraction (F2) had the highest activity in hydroxyl radical scavenging ability (62.61 ± 5.80%). The fraction F1 and F2 both exhibited good antioxidant activities in oxidative stress models in HUVECs and HaCaT cells. Among them, F2 could upregulate the activities of SOD and GSH-Px and reduce the lipid peroxidation degree to scavenge the ROS to protectCaenorhabditis elegansunder adversity. Then, 25 peptides total were identified from F2 by LC-MS/MS, and the peptide with the new sequence of INSSDVQGKY as the most significant component was synthetized and the ORAC assay and cellular ROS scavenging assay both illustrated its excellent antioxidant property.Keywords:phycobiliprotein;antioxidant peptide;bacterial extracellular protease;enzymatic hydrolyzation;C. elegans
研究领域:
生物化学
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分子生物学
·
海洋生物学
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产品关联
DOI
PubMed
高影响因子
Journal of CO2 Utilization
·
2020
·
IF 5.993
Yanxia Zhu,Jun Cheng,Ze Zhang,Jianzhong Liu
In order to promote phenol tolerance and the carbon fixation rate of cells during CO 2 sequestration in coal chemical flue gas, γ-ray irradiation from cobalt-60 ( 60 Co) was used to mutate Arthrospira platensis to generate efficient strains. A phenol-tolerant strain Mutant 11k was obtained with 11,000 Gy of gamma irradiation. The phenol degradation efficiency of the mutant was 34.7 % higher than that of wild strain, attributing to the increased activity of phenol hydroxylase that catalyzed phenol to generate catechol. The catechol 1,2-dioxygenase activity in mutant cells was enhanced by 39.5 % while catechol 2,3-dioxygenase was weakened by 23.8 %, revealing that the β-ketoadipate pathway plays a more important role than 2-hydroxymuconic semialdehyde pathway in phenol degradation. The activity of superoxide dismutase and catalase increased by 6.1 % and 39.8 %, respectively, reducing peroxide damage and maintaining rapid cell growth. The contents of chlorophyll-a and maximum quantum yield of photosystem II in mutant cells were higher than those in wild strain. The biomass yield and carbon fixation rate of mutant were 20.8 % and 22.4 % higher, respectively, than those of the wild strain.
研究领域:
环境科学
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微生物学
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生物技术
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产品关联
DOI
高影响因子
JOURNAL OF APPLIED PHYCOLOGY
·
2022
·Vol. 34
·期 3
·1497-1511
·
IF 3.404
Liu Jing,Bai Xinpeng,Fu Pengcheng
Arthrospira platensis proteins are considered as viable ingredients for functional foods with nutraceutical properties and health-promoting effects. In this study, we used five different proteases including pepsin, trypsin, alcalase, papain, and bromelain to hydrolyze A. platensis phycobiliprotein. LC–MS/MS (liquid chromatography-tandem mass spectrometry) was used to determine the composition of the hydrolysates with thousands of bioactive peptides. Based on peptide sequencing, a candidate list of 1,333 bioactive peptides was constructed for the first time for which bioinformatics tools were used to assess the properties of the bioactive peptides. The inhibitory activity of dipeptidyl peptidase IV (DPP-IV) and angiotensin-converting enzyme (ACE), as well as the antioxidant activity of five phycobiliprotein hydrolysates (PBPHs), were verified in vitro. The IC 50 values of PBPH-pepsin, PBPH-trypsin, PBPH-alcalase, PBPH-papain, and PBPH-bromelain for DPP-IV inhibited activity were determined to be 4.059, 5.603, 5.257, 3.819, and 4.195 mg mL −1 , respectively. All the above five PBPHs were also seen to significantly inhibit ACE activity ( P < 0.0001) in the range of 0.1–1.0 mg mL −1 . The activity of five PBPHs fraction was found for the 2-azino-bis (3-ethylbenzothiazoline6-sulfonic acid) diammonium salt (ABTS) assay (the highest was 1.37 mM Trolox equivalent of 30 mg mL −1 of PBPH-trypsin), 1,1diphenyl-2-picrylhydrazyl (DPPH) assay (the highest was 204.67 µM Trolox equivalent of 10 mg mL −1 of PBPH-pepsin), and Fe 2+ chelating ability (the highest was 639.73 µM FeSO 4 equivalent of 10 mg mL −1 of PBPH-trypsin). This study indicated that it was feasible to utilize A. platensis phycobiliprotein as a source of bioactive peptides which may be used as functional food and/or nutritional supplements for human health.
研究领域:
食品科学
·
生物化学
·
营养学
·
药理学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）