RSC Advances
·
2019
·Vol. 9
·期 29
·16701-16712
·
IF 3.049
Xiaofeng Li,Maohua Ma,Xuan Xin,Yuqian Tang,Guanglei Zhao,Xinglong Xiao
Gastrodin, a bioactive compound extracted from the plant source of Gastrodia elata Blume, has a wide range of therapeutic effects on central nervous system (CNS) diseases, but suffers from poor brain permeability and short half-life in plasma. In this study, fatty acid esters of gastrodin were successfully synthesized by a whole cell-based biocatalytic method. Aspergillus oryzae cells showed different catalytic activities in the organic solvent systems tested. Tetrahydrofuran was confirmed as the most suitable pure organic solvent, with the highest substrate conversion of 98.0%. Addition of ionic liquids (ILs) into pyridine dramatically accelerated the reaction with conversion increased from 5.9% to 84.2%, and also changed the selectivity of the cells, mainly due to the use of IL-containing systems altering cell permeability and contact of the enzymes with solvent molecules possessing different polarities. The ester products were characterized by 13C-NMR and ESI-MS as gastrodin monoester and diester.
研究领域:
药理学
·
生物催化
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Sustainable Materials and Technologies
·
2021
·
IF 7.053
C.H. Wang,L. Huang,Y. Zhong,X.L. Tong,C.D. Gu,X.H. Xia,Lingjie Zhang,X.L. Wang,J.P. Tu
High-performance lithium ion batteries (LIBs) with high energy/power density are always the persistent pursuit of people. High-quality anode is the key point for the performance improvement of LIBs. In this work, a facile combined strategy is proposed to construct integrated TNO/AOSC electrode, in which Aspergillus Oryzae Spore Carbon (AOSC) conductive matrix is employed to hold Ti 2 Nb 10 O 29 (TNO) nanoparticles . The TNO/AOSC not only shortens electron/ion transfer pathway on account of the nanostructure of TNO, but also provides better chemical stability from AOSC skeleton. Accordingly, the preeminent rate capacity (135.2 mA h g −1 at 10C) and relatively superior cycle stability (a capacity of 149.8 mA h g −1 after 200 cycles at 1C) are obtained for the TNO/AOSC, much better than TNO counterpart. The superior performance of the TNO/AOSC can be ascribed to the beneficial synergistic factors on enhanced electronic conductivity and solider structure from AOSC substrate. This intriguing design strategy may trigger interest for design of other high-quality electrodes in LIBs.
研究领域:
材料科学
·
能源存储
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2022
·
IF 11.504
Yan-Wei Liu,Qing-Hao Li,Guo-Qing Huang,Jun-Xia Xiao
The complexation between Aspergillus oryzae lipase (AOL) and chitosan (CS) was investigated as a function of phase separation pH and the resultant complexes were characterized. The results indicated that AOL and CS could assemble micron-sized complexes in pHs 5.0–6.5 and the strongest interaction occurred in pH 5.5. QCM-D analysis revealed that association in pH 5.5 generated a viscoelastic hydrated adlayer with the largest thickness. ITC analysis indicated that the interaction between AOL and CS was driven by enthalpy and the highest one-site binding stoichiometry was recorded in pH 5.5, which was 1.3 and 7.9 times higher than in pHs 5.0 and 6.0, respectively. Both electrostatic attraction and hydrophobic interaction played dominant role in AOL-CS complexation. Compared with free AOL, the interaction with CS reduced its α-helix content, increased its β-sheet content, and changed its tertiary structure; besides, the AOL-CS complex assembled in pH 5.5 exhibited the highest contact angle up to 85.5° and demonstrated enhanced lipase activity retention after incubation in pHs 3.0–9.0 and temperatures 35–75 °C. Hence, the AOL-CS complexes could be used to construct novel biphasic catalysis media based on O/W Pickering emulsions .
研究领域:
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
·
2013
·
IF 2.823
Jian-Yong Zheng,Jia-Yu Wu,Yin-Jun Zhang,Zhao Wang
The mycelium of Aspergillus oryzae WZ007 was successfully developed to kinetic resolution of ( R , S )-ethyl-2-(4-hydroxyphenoxy) propanoate (( R , S )-EHPP) for production of ( R )-ethyl-2-(4-hydroxyphenoxy) propanoate (( R )-EHPP). The key biocatalytic process parameters (pH, temperature, rotation speed and substrate concentration) were optimized. Under the optimum conditions, the optical purity of ( R )-EHPP was improved up to >99% when the conversion was above 49%. A. oryzae WZ007 whole-cell lipase exhibited high reaction capacity, enantioselectivity and good reusability. The tolerable substrate concentration was 0.5 mol/L, and dry mycelium of A. oryzae WZ007 maintains over 80% of its initial activity after eight repeating cycles. Therefore the enzymatic preparation of ( R )-EHPP route was suitable for industrial application.
研究领域:
生物化学
·
微生物学
·
制药工程
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Chi Zhao,Yuxiang Zhang,Shuangshuang Li,Jiayi Lin,Weifeng Lin,Weixin Li,Lixin Luo
To investigate the mechanism of flavor formation during the traditional preparation Cantonese soy sauce koji (TP), the changes of microorganisms, physicochemical properties, and flavor compounds in TP were comprehensively and dynamically monitored by absolute quantitative methods. Results demonstrated that inoculating Aspergillus oryzae 3.042 in TP was crucial role in enhancing enzyme activity properties. Absolute quantification of flavor combined with multivariate statistical analysis yielded 5 organic acids, 15 amino acids, and 2 volatiles as significantly different flavors of TP. Amplicon sequencing and RT-qPCR revealed that the dominant genera were Staphylococcus , Weissella , Enterobacter , Lactic streptococci , Lactobacillus , and Aspergillus , which exhibited a increasing trend in TP. Correlation analysis exhibited that Staphylococcus and Aspergillus were the pivotal genera contributing to the enzyme activities and flavor of TP. The flavor formation network involved lipid and protein degradation, carbohydrate metabolism and other pathways. Simultaneously, TP can appropriately increase the fermentation time to improve product quality.
研究领域:
食品科学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
·
2016
·Vol. 93
·期 10
·1347-1354
·
IF 1.505
Siyun Gu,Fengqin Feng,Yang Li,Wei Wei,Rongrong Lu
Dioleoyl; palmitoyl-rich triacylglycerols (OPO-rich TAG) were synthesized through Aspergillus oryzae lipase (AOL)-catalyzed acidolysis of palm stearin with commercial oleic acid by a one-step process in a stirred tank reactor and continuous packed bed reactor to evaluate the feasibility of using immobilized AOL. AOL was found to be valuable for the synthesis of OPO-rich TAG when compared with commercial lipase from Thermomyces lanuginose (Lipozyme ® TL IM; Novozymes A/S, Bagsvaerd, Denmark). The C52 (triglycerides with a carbon number of 52, stands for OPO, OPL, LPL and their isomers) content of AOL was higher (45.65 %), and the intensity of treatment (IOT: lipase amount × reaction time/TAG amount) of AOL was just 6.25 % of that of Lipozyme ® TL IM under similar reaction conditions in the stirred tank reactor. Response surface methodology were used to optimize the reaction conditions of the AOL-catalyzed acidolysis is reaction in the packed bed reactor. The optimal point for the set of experimental conditions generated were as follows: residence time 3.0 h; temperature 62.09 °C; substrate molar ratio 7.13 mol/mol. The highest C52 content obtained was 48.60 ± 2.36 %, with 57.46 ± 1.72 % total palmitic acid at the sn -2 position and 74.21 ± 2.45 % oleic acid at the sn -1,3 positions. The half-life of AOL was 24 h in the stirred tank reactor and 140 h in the packed bed reactor. The immobilized AOL achieved similar conversion and selectivity to commercial lipases for the catalyzed synthesis of OPO-rich TAG and may offer a cheaper alternative.
研究领域:
生物化学
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食品科学
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酶工程
✓ 已验证
产品关联
DOI
高影响因子
CATALYSIS COMMUNICATIONS
·
2013
·
IF 2.915
Hong-De Yan,Qun Zhang,Zhao Wang
A lipase from Aspergillus oryzae WZ007 exhibited high esterification activities toward a series of short-chain acids and alcohols. Moreover, it displayed a strong tolerance for high substrate concentration of up to 2.0 M and presented a highest initial rate of 276 mmol·L − 1 ·h − 1 at this concentration. After a reaction time of 48 h, the conversion rates of acids were higher than 80% for the synthesis of a majority of heptanoic acid esters, some octanoic acid esters and n -propyl hexanoate. These results implied that A. oryzae WZ007 lipase was a promising biocatalyst in the production of flavor esters.
研究领域:
生物化学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2017
·Vol. 52
·期 12
·2596-2603
·
IF 1.64
Lin Ge,Jingcong Xie,Tao Wu,Shanshan Zhang,Linguo Zhao,Gang Ding,Zhenzhong Wang,Wei Xiao
Summary A novel α-L-rhamnosidase was isolated and purified from Aspergillus oryzae NL-1. The enzyme was purified 13.2-fold by ultrafiltration, ion exchange and gel filtration chromatography with an overall recovery of 6.4% and specific activity of 224.4 U/mg, and the molecular mass of its subunit was approximately 75 kDa. Its optimal temperature and pH were 65 °C and 4.5, respectively. The enzyme was stable in the pH range 3.5–7.0, and it showed good thermostability at higher temperatures. The K M , kcat and kcat/K M values were 5.2 m m , 1624 s −1 and 312 s −1 m m −1 using p NPR as substrates, respectively. Moreover, the enzyme exhibited transglycosylating activity, which could synthesise rhamnosyl mannitol through the reactions of transglycosylation with inexpensive rhamnose as the glycosyl donor. Our findings indicate that the enzyme has potential value for glycoside synthesis in the food industry.
研究领域:
生物化学
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
·
2016
·Vol. 64
·期 2
·251-258
·
IF 1.429
Hong-De Yan,Zhao Wang,Jun-Qing Qian
A mycelium-bound lipase from Aspergillus oryzae (AOL) exhibited excellent enantioselectivity for kinetic resolution of ( RS )-1-phenylethanol (( RS )-1-PE) in organic solvent. The various reaction parameters affecting the conversion and enantioselectivity were studied, including type of acyl donor, solvent, molar ratio, temperature, enzyme amount, and substrate concentration. The optimum reaction conditions were found to be transesterification with vinyl acetate at 30 °C in methyl tert -butyl ether with a vinyl acetate: ( RS )-1-PE molar ratio of 1:1 and an enzyme concentration of 60 g/L. At the optimum reaction conditions, the conversion could reach above 46% with >99% enantiomeric excess of the product, ( R )-1-phenylethyl acetate, when the substrate concentration was below 1.4 M. The enzyme displayed an excellent enantioselectivity with an E -value of >200 and a strong tolerance for high substrate concentration of up to 1.8 M. Those results indicated that AOL was a promising biocatalyst in the kinetic resolution of ( RS )-1-PE.
研究领域:
生物化学
·
酶工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jinhong Gao,Lingli Zhang,Dongxin Zhao,Xin Lu,Qiang Sun,Heng Du,Hongyan Yang,Kui Lu
Aspergillus oryzae β-D-galactosidase (β-Gal) efficiently hydrolyzes sesaminol triglucoside into sesaminol, which has higher biological activity . However, β-Gal is difficult to be separate from the reaction mixture and limited by stability. To resolve these problems, β-Gal was immobilized on amino-functionalized magnetic nanoparticles mesoporous silica pre-activated with glutaraldehyde (Fe 3 O 4 @mSiO 2 -β-Gal), which was used for the first time to prepare sesaminol. Under the optimal conditions, the immobilization yield and recovered activity of β-Gal were 57.9 ± 0.3 % and 46.5 ± 0.9 %, and the enzymatic loading was 843 ± 21 U enzyme /g support . The construction of Fe 3 O 4 @mSiO 2 -β-Gal was confirmed by various characterization methods, and the results indicated it was suitable for heterogeneous enzyme-catalyzed reactions. Fe 3 O 4 @mSiO 2 -β-Gal was readily separable under magnetic action and displayed improved activity in extreme pH and temperature conditions. After 45 days of storage at 4 °C, the activity of Fe 3 O 4 @mSiO 2 -β-Gal remained at 92.3 ± 2.8 %, which was 1.29 times than that of free enzyme, and its activity remained above 85 % after 10 cycles. Fe 3 O 4 @mSiO 2 -β-Gal displayed higher affinity and catalytic efficiency. The half-life was 1.41 longer than free enzymes at 55.0 °C. Fe 3 O 4 @mSiO 2 -β-Gal was employed as a catalyst to prepare sesaminol, achieving a 96.7 % conversion yield of sesaminol. The excellent stability and catalytic efficiency provide broad benefits and potential for biocatalytic industry applications.
研究领域:
生物化学
·
酶工程
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2022
·
IF 6.064
Yang Rongling,Wang Yu,Zhao Xiangjie,Tong Zheng,Zhu Qianlin,He Xiaoxi,Wang Zhaoyu,Luo Hongzhen,Fang Fang
Salidroside, the main bioactive compound isolated from the plant source of Rhodiola rosea L, possesses broad-spectrum pharmacological activities, but suffers from the low cell membranes permeability and alimentary absorption due to its high polarity. Therefore, a whole-cell catalytic strategy for the synthesis of salidroside esters was explored to improve its lipophilicity. The results showed that Aspergillus oryzae demonstrated the highest biocatalytic activity among the microbial strains tested. For the synthesis of salidroside caprylate, the optimum conditions of reaction medium, Aspergillus oryzae amount, molar ratio of vinyl caprylate to salidroside and reaction temperature were acetone, 30 mg/ml, 10°C and 40°C, respectively. Under these conditions, the initial reaction rate was 15.36 mM/h, and substrate conversion and regioselectivity all reached 99%. Moreover, the results indicated that although various 6′-monoesters derivatives of salidroside were exclusively obtained with excellent conversions (96%–99%), the reaction rate varied greatly with different chain-length acyl donors. This study details an efficient and cost-effective biocatalytic approach for the synthesis of salidroside esters by using Aspergillus oryzae as a catalyst for the first time. Considering the whole cell catalytic efficiency and operational stability, this strategy may provide a new opportunity to develop green industrial processes production for ester derivatives of salidroside and its analogues.
研究领域:
药理学
·
生物化学
·
工业微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2023
·Vol. 332
·期 6
·2149-2158
·
IF 1.6
He Yizhou,Wang Yazhou,Cai Chengkun,Yang Guolin,Zhou Li,Ran Gang,Chen Tao,Zhu Wenkun
Microbial fermentation pretreatment is an effective strategy to solve the problem of low uranium adsorption capacity of traditional biochar materials by introducing some porous structures and special functional groups in the fermentation process. Here, five different microorganisms ( Bacillus licheniformis , Aspergillus oryzae , Lactobacillus acidophilus , Bacillus subtilis, and Saccharomyces cerevisiae ) were selected to pretreated cotton stalk biochar to find the most suitable microorganism to increase surface area and effective functional groups. Cotton stalk biochar fermented by Aspergillus oryzae has a highest specific surface area and more functional groups than other groups. U(VI) adsorption efficacy of five fermentation biochars and blank control was tested for different contact times and initial concentrations of U(VI). Cotton stalk biochar fermented by Aspergillus oryzae had the best uranium adsorption performance with an adsorption capacity of 100.31 mg/g. Mechanism analysis showed that the adsorption of U(VI) on cotton stalk biochar fermented by Aspergillus oryzae was mainly attributed to the ion exchange with Ca 2+ and the complexation with phosphate groups. Based on the above analysis, cotton stalk biochar fermented by Aspergillus oryzae possessed the potential to be applied for removing U(VI) from wastewater.
研究领域:
环境科学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）