The antifungal activity and encapsulation properties of cinnamaldehyde-loaded microcapsules by tannic acid were investigated. The results showed that cinnamaldehyde exhibited a high in vitro antifungal effect against Aspergillus brasiliensis through altering the morphology of the hyphae, and its inhibition zone diameter (IZD) exceeded 15 mm with a minimum inhibitory concentration of 1.0 mg/mL. Cinnamaldehyde-loaded microcapsules crosslinked with tannic acid have a high encapsulation efficiency (92.53 ± 5.20%) and antifungal activity (37.82 ± 0.63 mm), and the retention rate was 2.5 times of that of unencapsulated cinnamaldehyde after 30-d storage at 37 °C. Furthermore, the antifungal stability was enhanced (IZD 25.37 mm) after 60-d storage at 37 °C as compared with that of cinnamaldehyde (IZD 22.07 mm). The results of cell membrane integrity and the extracellular protein content also indicated that the microencapsulation could effectively protect cinnamaldehyde, delay the decrease of antifungal effect and improve its long-term antifungal activity against A. brasiliensis by increasing the retention rate of cinnamaldehyde after storage.
Bingjie Liu,Xinshuo Wang,Lin Lu,Quan Wen,Xiaoming Zhang,Caleb John Swing,Shuqin Xia
Summary The evaluation of antifungal properties in cinnamaldehyde (CA)-loaded microcapsules is significant to clarify potential food applications in various food matrices and systems. The impact of tannic acid (TA) crosslinking on the release and the antifungal properties of CA microcapsules was investigated based on gelatin/gum Acacia complex coacervates. The inhibition zone diameter of microencapsulated CA against Aspergillus brasiliensis was dependent on the TA addition and retained 26.1 ± 0.1 mm after 180-day storage at 25 °C. Specifically, TA enhanced hydrogen bond interactions between the microcapsule wall materials and favoured a sustained release of CA during the incubation with Aspergillus brasiliensis. Meanwhile, confocal laser scanning microscopic observation revealed that the microstructure of multinuclear microcapsules was more compact and intact under the crosslinking of 0.4% TA. The comparation of microscopic morphology and membrane potential of Aspergillus brasiliensis exerted a synergistic antifungal effect between CA and TA crosslinked microcapsules.
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