BIOPROCESS AND BIOSYSTEMS ENGINEERING
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2025
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IF 3.6
Yi Xia,Li Xinji,Han Jianqi,Liu Zhidan,Shi Xiaohui,Wen Tao,Zhu Jie
Itaconic acid can be produced using lignocellulosic biomass; however, the inhibitors from pretreatment process of biorefinery are toxic to the fermenting strains. Here, with 35.70 ± 0.69 g/L (0.19 ± 0.05 g/L·h and 73.84 ± 0.01%) itaconic acid from shake flask fermentation of synthetic medium (SM), a newly isolated Aspergillus terreus just produced 1.01 ± 0.01 g/L itaconic acid from corn stover hydrolysates (CSH) for the serious block of aldehyde inhibitors and acetic acid. Convincingly, 25.34 ± 3.94 g/L (0.13 ± 0.02 g/L·h and 37.92 ± 3.89%) itaconic acid was achieved from the detoxified CSH (with residual 0.49 g/L acetic acid) using 4.0% activated charcoal. 21.64 ± 2.42 g/L (0.05 ± 0.01 g/L·h and 26.96 ± 7.81%) itaconic acid was further achieved from CSH for the adapted A. terreus with better degradation ability of furanic aldehydes and phenolic aldehydes. Furthermore, the 108 mutation sites of nine genes from adaptive laboratory evolution (ALE) for A. terreus were further uncovered through single nucleotide polymorphisms (SNPs) analysis, and thus would be responsible for the improved fermentability of itaconic acid from CSH. The current work broke the bottlenecks in itaconic acid fermentation directly from CSH through improving A. terreus using directed evolution technique, and thus would provide a strain biocatalyst A. terreus and establish the alternative strategy to efficiently produce itaconic acid using corn stover. Graphical abstract
研究领域:
生物技术
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发酵工程
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产品关联
DOI
PubMed
高影响因子
Chinese Journal of Natural Medicines
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2019
·
IF 1.773
Xiao-Wei LUO,Yun LIN,Yong-Jun LU,Xue-Feng ZHOU,Yong-Hong LIU
Two new isomeric modified tripeptides, aspergillamides C and D (compounds 1 and 2 ), together with fifteen known compounds (compounds 3–17 ), were obtained from the marine sponge-derived fungus Aspergillus terreus SCSIO 41008. The structures of the new compounds, including absolute configurations, were determined by extensive analyses of spectroscopic data (NMR, MS, UV, and IR) and comparisons between the calculated and experimental electronic circular dichroism (ECD) spectra. Butyrolactone I (compound 11 ) exhibited strong inhibitory effects against Mycobacterium tuberculosis protein tyrosine phosphatase B (MptpB) with the IC 50 being 5.11 ± 0.53 μmol·L −1 , and acted as a noncompetitive inhibitor based on kinetic analysis.
研究领域:
药物化学
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微生物学
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有机化学
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DOI
PubMed
高影响因子
Marine Drugs
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2019
·Vol. 17
·期 6
·332
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IF 3.772
Zhongbin Cheng,Yuanli Li,Wan Liu,Lijun Liu,Jie Liu,Wangjun Yuan,Zhuhua Luo,Wei Xu,Qin Li
Three new butenolide derivatives, namely aspernolides N–P (1–3), together with six known analogues (4–9), were isolated from the ethyl acetate (EtOAc) extract of the deep sea-derived fungusAspergillus terreusYPGA10. The structures of compounds1–3were determined on the basis of comprehensive analyses of the nuclear magnetic resonance (NMR) and mass spectroscopy (MS) data, and the absolute configurations of1and2were determined by comparisons of experimental electronic circular dichroism (ECD) with calculated ECD spectra. Compound1represents the rare example ofAspergillus-derived butenolide derivatives featured by a monosubstituted benzene ring. Compounds6–9exhibited remarkable inhibitory effects againstα-glucosidase with IC50values of 3.87, 1.37, 6.98, and 8.06 μM, respectively, being much more active than the positive control acarbose (190.2 μM).Keywords:Aspergillus terreusYPGA10;deep-sea-derived fungus;butenolide derivatives;α-glucosidase
研究领域:
微生物学
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药物化学
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DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2022
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IF 6.449
Yixin Sun,Peishan Li,Qi Sun,Xin Liu,Yanping Wang,Bixian Zhang,Shijia Dong,Xiaomei Hu
Ionic liquids (ILs)-assisted microbes and enzymes are potential approaches for converting lignocellulosic biomass into fermentable sugars in a biorefinery. In the present study, an ILs-tolerant strain Aspergillus terreus NEAU-7 with ILs-stable cellulase production ability was isolated and identified. A comparative transcriptome analysis revealed that A. terreus NEAU-7 could promote ion transport and acquire metabolic energy to reduce the adverse effect of ILs. The results further confirmed that the strain could increase the accumulation of compatible solutes to offset the osmotic pressure of ILs and reduce the cell membrane permeability to prevent ILs. A secretome analysis of Carbohydrate-Active Enzymes (CAZymes) showed no significant reduction in the Glycoside Hydrolases (GH) families of cellulolytic enzymes, while a significant reduction was observed for Carbohydrate Esterases (CE) families and Glycoside Hydrolase 43 (encoding α- L -arabinofuranosidases and β-xylosidases), indicating that the presence of ILs could decrease the expression of hemicellulolytic enzymes. Herein, a model for lignocellulose degradation of A. terreus NEAU-7 was interpreted. Overall, this study will provide insights into further exploration of the ILs-resistance mechanism of lignocellulolytic fungi and employment of ILs-assisted fungi in the degradation of lignocellulosic biomass into value-added products.
研究领域:
生物技术
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微生物学
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酶工程
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Chemistry
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2022
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IF 5.545
Cui Xiang,Deng Shanshan,Li Guoyin,Zhang Yunxia,Wang Lining,Wu Changjing,Deng Yanru
Two undescribed butenolide derivatives, asperteretal J (1) and K (2), together with 13 known ones (3–15) were isolated from an endophytic fungus Aspergillus terreus SGP-1, the fermentation product of which exhibited selective inhibitory activity toward butyrylcholinesterase. The structures of the new compounds were elucidated based on HRMS and NMR data, and the absolute configurations were determined by specific optical rotation comparison. All compounds were evaluated for cholinesterase inhibitory effects with galantamine as a positive control. Compounds 4–8 selectively inhibited butyrylcholinesterase with IC50 values of 18.4–45.8 µM in a competitive manner, with Ki values of 12.3–38.2 µM. The structure-activity relationship was discussed. Molecular docking and dynamic simulation of the inhibitor-enzyme complex were performed to better understand the interactions.
研究领域:
药理学
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生物化学
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真菌学
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药物化学
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产品关联
DOI
PubMed
高影响因子
Natural Products Journal
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2024
·Vol. 14
·期 3
·78-83
·
IF 0.7
Tian Danmei,Chen Mei,Ding Wenjuan,Li Jia,Wu Shi,Hong Kui,Tang Jinshan
Background: Marine fungi have been proven to be a new arsenal for the discovery of valuable secondary metabolites. Method: Fungus strain Aspergillus terreus DM62 was initially fermentated using solid corn medium and PDA liquid medium. Subsequently, extensive chromatographic methods
were applied to isolate the fermentation cultures of DM62, and the chemical structures of isolate compounds were elucidated by pectroscopic analyses and optical rotations comparison. Additionally, α-glucosidase and ATPcitrate lyase (ACL) inhibitory activities of isolate compounds were
assessed to investigate their hypoglycemic and lipid-lowering activities. Result: A new cinnamate derivative, plicati n C (1), together with 18 known compounds, were isolated from the solid and liquid state fermentations of A. terreus DM62. Activity evaluation showed that compounds 3,
6, and 8-10 exhibited stronger α-glucosidase inhibitory activities than acarbose at 400 μM, and butenolide 3, with an IC50 value of 21.5 μM, was discovered with significant ACL inhibitory activity for the first time. Conclusion: This study not only discovered a
new cinnamate derivative but also found butenolides with potent ACL inhibitory activity, which is favorable to clarify their pharmacological mechanism in the treatment of metabolic disease.
研究领域:
药理学
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天然产物化学
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生物化学
✓ 已验证
DOI
高影响因子
BIORESOURCE TECHNOLOGY
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2020
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IF 7.539
Jing Yang,Hao Xu,Jianchun Jiang,Ning Zhang,Jingcong Xie,Jian Zhao,Quan Bu,Min Wei
Itaconic acid (IA) production by fermentation of undetoxified hydrolysate of bamboo residues by Aspergillus terreus was demonstrated. Monosaccharides were obtained by pretreatment and enzymatic hydrolysis of bamboo residues. A. terreus could not grow and synthesize IA in the hydrolysate. The buffer was confirmed to be an inhibitor, and was successfully replaced by deionized water as the suspension, to release equivalent sugar and eliminate the inhibition. Corn steep liquor significantly improved the adaptability of A. terreus to the hydrolysate at 2.0 g/L. The IA titer obtained (19.35 g/L IA) was the highest to be reported for IA production from lignocellulose without detoxification. Simultaneous saccharification and fermentation and fed-batch fermentation increased the titer to 22.43 g/L and 41.54 g/L, respectively. Meanwhile, economic assessment proved that bamboo residues were potential substrates for IA production with economic effectiveness.
研究领域:
生物技术
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生化工程
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环境科学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Chemistry
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2021
·
IF 5.221
Cao Jia-Ren,Fan Fang-Fang,Lv Chang-Jiang,Wang Hong-Peng,Li Ye,Hu Sheng,Zhao Wei-Rui,Chen Hai-Bin,Huang Jun,Mei Le-He
Transaminases that promote the amination of ketones into amines are an emerging class of biocatalysts for preparing a series of drugs and their intermediates. One of the main limitations of (R)-selective amine transaminase from Aspergillus terreus (At-ATA) is its weak thermostability, with a half-life (t1/2) of only 6.9 min at 40°C. To improve its thermostability, four important residue sites (E133, D224, E253, and E262) located on the surface of At-ATA were identified using the enzyme thermal stability system (ETSS). Subsequently, 13 mutants (E133A, E133H, E133K, E133R, E133Q, D224A, D224H, D224K, D224R, E253A, E253H, E253K, and E262A) were constructed by site-directed mutagenesis according to the principle of turning the residues into opposite charged ones. Among them, three substitutions, E133Q, D224K, and E253A, displayed higher thermal stability than the wild-type enzyme. Molecular dynamics simulations indicated that these three mutations limited the random vibration amplitude in the two α-helix regions of 130–135 and 148–158, thereby increasing the rigidity of the protein. Compared to the wild-type, the best mutant, D224K, showed improved thermostability with a 4.23-fold increase in t1/2 at 40°C, and 6.08°C increase in T5010. Exploring the three-dimensional structure of D224K at the atomic level, three strong hydrogen bonds were added to form a special “claw structure” of the α-helix 8, and the residues located at 151–156 also stabilized the α-helix 9 by interacting with each other alternately.
研究领域:
生物技术
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酶学
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蛋白质工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOTECHNOLOGY
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2014
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IF 2.884
Chengli Hu,Na Wang,Weiwei Zhang,Sheng Zhang,Yanfa Meng,Xiaoqi Yu
The aim of this study was to improve the ability of Aspergillus terreus lipase to separate the racemic ketoprofen vinyl ester into individual enantiomers using hollow self-assembly alginate- graft -poly(ethylene glycol)/α-cyclodextrins (Alg- g -PEG/α-CD) spheres as enzyme immobilization carriers. The morphology and size of the Alg- g -PEG/α-CD particles were investigated by transmission electron microscopy (TEM) and were found to be nanoscale. To facilitate recycling, calcium alginate (CA) beads were developed to encapsulate Alg- g -PEG/α-CD particles, thereby producing Alg- g -PEG/α-CD/CA composite beads. The influence of buffer pH and enzyme concentration during immobilization was studied along with the biocatalyst's kinetic parameters. When the immobilized enzyme was under optimal conditions in the resolution reaction, maximal conversion (approximately 45.9%) and enantioselectivity (approximately 128.8) were obtained. The immobilized A. terreus lipase maintained excellent performance even after 20 reuses and retained nearly 100% of its original activity after 24 weeks of storage at 4 °C.
研究领域:
生物化学
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酶工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MYCOBIOLOGY
·
2017
·
IF 0.761
Qiang Zhang,Yujuan Li,Fangxue Xu,Mengmeng Zheng,Xiaozhi Xi,Xuelan Zhang,Chunchao Han
Different endophytes isolated from the seeds of Sophora flavescens were tested for their ability to produce matrine production. Response surface methodology (RSM) was applied to optimize the medium components for the endophytic fungus. Results indicated that endophyte Aspergillus terreus had the ability to produce matrine. The single factor tests demonstrated that potato starch was the best carbon source and the combination of peptone and NH 4 NO 3 was the optimal nitrogen source for A. terreus . The model of RSM predicted to gain the maximal matrine production at 20.67 µg/L, when the potato starch was 160.68 g/L, peptone was 24.96 g/L and NH 4 NO 3 was 2.11 g/L. When cultured in the optimal medium, the matrine yield was an average of 20.63 ± 0.11 µg/L, which was consistent with the model prediction. This study offered an alternative source for the matrine production by endophytic fungus fermentation and may have far-reaching prospect and value.
研究领域:
微生物学
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天然产物化学
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产品关联
DOI
PubMed
高影响因子
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