PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
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2023
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IF 2.7
Qi Xin,Li Wang,Haiyan Ruan,Ling Liu,Si Sun,Jing Zhang,Menglin Wu,Xiaoyu Mu,Hao Wang,Shaofang Zhang,Xiao-Dong Zhang
Redox-active nanozymes offer low-cost controlled synthesis, high stability, and tunable catalytic properties over natural enzymes, which have attracted wide attention in the field of disease diagnosis and treatment. However, the improvement of catalytic activity remains an important challenge for nanozymes. Herein, the Au/CeO 2 nanozymes is developed to achieve enhanced multiple enzyme-mimetic activity. The Au/CeO 2 nanozymes at 5% doping possess best peroxidase-like activity with threefold higher catalytic rate than CeO 2 . For catalase-mimic catalysis, the Au/CeO 2 nanozymes at 5% doping also exhibited a 1.5-fold enhanced reaction rate higher than pure CeO 2 . The superoxide dismutase (SOD)-like capacity of Au/CeO 2 nanozymes is proportional to Au content. The Au/CeO 2 nanozymes at 10% doping show optimal SOD-like capacity of 60.2 U mg −1 . In vitro experiments validate the regulation ability of intracellular oxidative stress and inflammation. Au/CeO 2 nanozymes can reduce lipopolysaccharide- or H 2 O 2 -induced oxidative damage by scavenging excess ROS in nerve cell. Therefore, Au/CeO 2 can be used as a promising antioxidant in disease treatment, and the study offers general guidelines for achieving enhanced biocatalytic property through atomic doping.
研究领域:
纳米技术
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生物催化
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疾病诊断与治疗
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
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2024
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IF 8.3
Qi Xin,Shaofang Zhang,Si Sun,Nan Song,Yadong Zhe,Fangzhen Tian,Shu Zhang,Meili Guo,Xiao-Dong Zhang,Jianning Zhang,Hao Wang,Ruiping Zhang
Sepsis, a life-threatening condition caused by a dysregulated immune response to infection, leads to systemic inflammation, immune dysfunction, and multiorgan damage. Various oxidoreductases play a very important role in balancing oxidative stress and modulating the immune response, but they are stored inconveniently, environmentally unstable, and expensive. Herein, we develop multifunctional artificial enzymes, CeO2 and Au/CeO2 nanozymes, exhibiting five distinct enzyme-like activities, namely, superoxide dismutase, catalase, glutathione peroxidase, peroxidase, and oxidase. These artificial enzymes have been used for the biocatalytic treatment of sepsis via inhibiting inflammation and modulating immune responses. These nanozymes significantly reduce reactive oxygen species and proinflammatory cytokines, achieving multiorgan protection. Notably, CeO2 and Au/CeO2 nanozymes with enzyme-mimicking activities can be particularly effective in restoring immunosuppression and maintaining homeostasis. The redox nanozyme offers a promising dual-protective strategy against sepsis-induced inflammation and organ dysfunction, paving the way for biocatalytic-based immunotherapies for sepsis and related inflammatory diseases.
研究领域:
纳米技术
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免疫学
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炎症
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治疗学
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生物催化
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2024
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IF 8.3
Qi Zhong,Kangdong Wang,Gao Pan,Shuping Peng,Cijun Shuai
CeO2 nanozymes have demonstrated the potential to enhance biological scaffolds with chemodynamic therapy. However, their catalytic efficacy is limited by the slow conversion of Ce4+ to Ce3+ and the lack of substrates like H2O2 and H+. To address these challenges, we adopted a dual-pronged strategy that utilized the plasmonic resonance of Au nanoparticles and their glucose-oxidase mimicry to boost electron and hydrogen transfer. Specifically, we integrated Au/CeO2 nanozymes into poly-l-lactic acid scaffolds via selective laser sintering. This conversion of Ce3+ to Ce4+ in the scaffolds enhanced the reduction of H2O2 to a hydroxyl radical, inducing oxidative stress in tumor cells. The Au nanoparticles played a crucial role in boosting the Ce3+/Ce4+ catalytic cycle by providing both the energy and the catalytic substrates. They recycled Ce4+ back to Ce3+ by exploiting plasmonic-induced hot electrons and catalyzed glucose oxidation to supply H2O2 and H+. Our nanoscale and atomic-scale simulations confirmed that the Au/CeO2 hybrid structure utilized near-field coupling to amplify the plasmonic resonance and the Au–O–Ce bridge reduced the electron transfer barrier. Consequently, the Au/CeO2 scaffold decreased the activation energy from 22.57 to 9.92 kJ/mol. These findings highlight the significant promise of the Au/CeO2 nanozyme scaffold for NIR-enhanced chemodynamic therapy.
研究领域:
纳米技术
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癌症治疗
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材料科学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）