MICROCHIMICA ACTA
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2025
·Vol. 192
·期 9
·1-13
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IF 5.3
Kang Jia,Qi Meng,Wang Yasen,Li Linchun,Zhou Xinda,An Xiaoshuo,Hammock Bruce D.,Zhang Jinlin,Huo Jingqian
Nanobodies specifically recognizing atrazine were successfully obtained through immunization of alpacas and phage display libraries, and an indirect competitive immunoassay (ic-ELISA) based on nanobodies was constructed with an IC 50 of 0.062 μg/mL and a minimum detection limit of 0.01 μg/mL. An Au@PtNPs with excellent colorimetric performance, high catalytic activity, and high stability was synthesized. The combination of Au@PtNPs and nanobodies led to the development of a direct competitive immunoassay (dc-ELISA) with higher specificity, convenience, lower cost and an IC 50 value of 0.032 μg/mL which was lower than the ic-ELISA. Based on the Au@PtNPs and nanobodies, a novel LFIA model for qualitative and semi-quantitative detection of atrazine was constructed with a visual detection limit of 5 ng/mL. The present study is of great significance for the construction of atrazine immunoassay combining nanobodies and nanoenzymes, which provides a sensitive, specific, simple and reliable method for the detection of atrazine in the environment.
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研究领域:
分析化学
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免疫学
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纳米科技
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环境科学
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生物化学
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PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
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2025
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IF 2.5
Yuying Jia,Xiangyu Cai,Xuepeng Wang,Hongyan Shan,Yanfu Huan
Accurate detection of Cys/Hcy levels is critical for evaluating human health conditions. In this study, we developed an innovative fluorescence/colorimetric dual-mode sensing system to detect Cys/Hcy by dynamically adjusting the level of oxTMB. The system utilizes Au@PtNPs with peroxidase-like activity to catalyze the oxidation of colorless 3,3,5,5- tetramethylbenzidine (TMB) to blue oxTMB, and TAPB@DMTP COF, a fluorescent nanomaterial with abundant spatial pores, could be used as a carrier for oxTMB, and the two were made fluorescence quenching due to the fluorescence inner filter effect. The addition of Cys/Hcy allowed the originally dispersed Au@PtNPs to aggregate to inhibit their peroxidase-like activity, thus indirectly regulating the oxTMB content. At this point, the absorbance signal decreased and fluorescence was restored. To this end, we have developed a special fluorescence/colorimetric dual-mode sensing system for the detection of Cys/Hcy. The detection limits of the fluorescence and colorimetric methods for Cys/Hcy were determined to be 0.788 nM (Cys), 0.663 nM (Hcy), and 1.795 nM (Cys), 1.886 nM (Hcy), respectively. The sensing system was also evaluated for its ability to withstand interferences from 12 anions and 13 amino acids, demonstrating excellent unti-interference and selectivity. The recoveries of the two methods for detecting Cys/Hcy in fetal bovine serum were found to be 98.9%-114.3% with RSDs as low as 0.52% (fluorescence) and 0.92% (colorimetric), respectively.
研究领域:
生物化学
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分析化学
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医学诊断
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DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2021
·
IF 4.464
Shanshan Wei,Haolin Xiao,Miao Gu,Zhencheng Chen,Liangli Cao
In this study, a sensitive and quantitative label-free electrochemical immunosensor based on bimetallic [email protected] nanoparticles , Prussian blue (PB), and reduced graphene oxide-tetraethylene pentamine nanocomposite (rGO-TEPA) was constructed to achieve sensitive detection of HBsAg. A one-pot green method was used to synthesize interlocked rGO-TEPA/PB hybrid composites as high-performance materials with good conductivity . PB was the electron transfer mediator used to provide electrochemical signals. By utilizing rGO-TEPA as supporting material , the stability and intensity of PB were greatly improved. The incorporation of highly conductive [email protected] nanoparticles could further amplify the electrochemical signal, and the high adsorption capacity could provide an efficient substrate for hepatitis B surface antibody (HBsAb) immobilization. Under optimal conditions, the immunoassay had a wide linear range from 0.25 pg mL −1 to 400 ng mL −1 and a low detection limit of 0.08 pg mL −1 (S/N = 3). This method was also applied to the analysis of human serum samples, and the obtained results were on par with those of electrochemiluminescence immunoassay (ECLIA), suggesting significant potential for this new method in clinical research.
研究领域:
生物医学工程
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分析化学
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临床诊断
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2025
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IF 7.7
Yayun Jiang,E Jianfei,Youwei Li,Jian Chen,Jie Yang,Yang He,Gang Mai,Laibao Zheng
Group B Streptococcus (GBS) is a major cause of neonatal morbidity and mortality, underscoring the urgent need for rapid, accurate, and deployable diagnostics. Conventional culture and nucleic acid amplification tests are limited by long turnaround time, insufficient sensitivity, or dependence on sophisticated instrumentation, restricting their use in resource-limited settings. Here, we present a magnetically controlled dual-mode biosensing platform, termed c olorimetric/ p hotothermal–CRISPR/ Cas – Au @ Pt nanozyme (CP-CasAuPt), which integrates the sequence-specific trans-cleavage activity of CRISPR/Cas12a with the peroxidase-mimicking activity of Au@PtNPs. The catalytic oxidation of 3,3′,5,5′-Tetramethylbenzidine (TMB) generates oxidized TMB (oxTMB), which provides a visible colorimetric change and a near-infrared photothermal response, thereby enabling reliable dual-mode readouts with minimal instrumentation. Magnetic bead-assisted separation further reduces background signals, ensuring robust performance in complex matrices. Using GBS as a proof-of-concept, CP-CasAuPt achieved a detection limit of 10 3 CFU/mL after loop-mediated isothermal amplification, with results fully consistent with qPCR across 20 clinical samples. Beyond high sensitivity and specificity, this study establishes a generalizable strategy for multimodal molecular diagnostics, where orthogonal dual signals allow cross-validation, ensuring robust detection across diverse assay conditions. The CP-CasAuPt system thus offers a promising paradigm for rapid, instrument-free pathogen detection, particularly suited for resource-limited settings.
研究领域:
分子诊断
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生物传感器
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纳米技术
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CRISPR技术
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即时检测
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传染病检测
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高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 10
·1-10
·
IF 6.232
Cao Liangli,Xiao Haolin,Fang Cheng,Zhao Feijun,Chen Zhencheng
Using gold and magnetic nanoparticles co-decorated reduced graphene oxide-tetraethylenepentamine (rGO-TEPA/Au-MNPs) as the magnetic platform for capturing the primary antibody (Ab 1 ), separation and preconcentration of immunocomplex, a novel homogeneous electrochemical immunosensor was successfully developed. The newly prepared magnetic rGO-TEPA/Au-MNPs, compared with MNPs, exhibited better stability and enhanced electrical conductivity attributed to rGO-TEPA, and showed higher biorecognition efficiency due to AuNPs. In addition, Au@PtNPs were prepared and modified with secondary antibody (Ab 2 ) as an efficient signal probe for signal readout. Using carcinoembryonic antigen (CEA) as a model analyte, the prepared immunosensor demonstrated satisfactory properties like high stability, good repeatability and selectivity, wide linear range (5.0 pg mL −1 ~200.0 ng mL −1 ) as well as low detection limit (1.42 pg mL −1 ). The homogenous electrochemical immunosensor was applied to the detection of CEA in human serum and was found to exhibit good correlation with the reference method. Thus, the proposed rGO-TEPA/Au-MNPs-based homogenous immunoassay platform might open up a new way for biomarker diagnosis.
Graphical Abstract
研究领域:
分析化学
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生物传感器
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纳米技术
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生物医学工程
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癌症诊断
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Junkui Li,Chen Li,Wang Guo,Yiqing Guo,Xiaobo Zou,Zongbao Sun
The development of a capsaicin detection system that integrates facile preliminary evaluation with high sensitivity and accuracy across a wide detection range is crucial for the discrimination of gutter oil and the protection of human health. In this study, we constructed a novel colorimetric/SERS dual-mode detection system utilizing peroxidase-like Au@Pt nanoparticles (NPs) that exhibit SERS-activity. The Au@Pt NPs feature a spherical gold nanoparticle core with synapse-like platinum nanoparticles distributed intermittently on their surface, this structure enhances the catalytic activity of the PtNPs and the SERS activity of the AuNPs, making them ideal for designing colorimetric/SERS dual-mode probes. Based on the colorimetric assay, we achieved a capsaicin detection range from 1 to 10,000 μM, with a limit of detection (LOD) of 3.48 μM, as determined by absorption spectroscopy analysis. Additionally, a linear calibration curve for capsaicin ranging from 10 −9 to 10 −5 M was established using the SERS method, yielding an LOD of 7.38 nM. By integrating the advantages of facile detection through colorimetric analysis with the high-sensitivity trace assay provided by SERS, this proposed dual-mode detection system demonstrates excellent sensitivity, reliability, selectivity, and wide detection range compared to previous probes and methods. Consequently, this method can serve as an efficient tool for monitoring oil quality.
研究领域:
分析化学
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食品安全
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纳米技术
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Zhengtao Li,Lingjun Geng,Jiashuai Sun,Jingcheng Huang,Rui Xu,Zhen Guo,Shouyi Dou,Liying Zhang,Fangling Du,Yemin Guo,Xia Sun,Nortoji A. Khujamshukurov
Au@PtNPs displayed superiority in antibody binding efficiency and signal intensity. • Multifunctional Au@PtNPs were employed to construct dual-signal ICTS. • Au@PtNPs-ICTS demonstrated excellent performance in thiamethoxam detection. • Au@PtNPs-ICTS could change identification components to detect different targets. Immunochromatographic test strip (ICTS) is a rapid analytical technique widely used in environmental and food detection owing to its merits of simple operation and short analysis time. Herein, three-dimensional nanoflower-structured gold‑platinum nanoparticles (Au@PtNPs) were synthesized via a seed-growth method. Compared with conventional gold nanoparticles (AuNPs), Au@PtNPs exhibited stronger signal intensity, excellent catalytic performance, and efficient antibody binding efficiency. Colorimetric Au@PtNPs-ICTS and catalytic colorimetric Au@PtNPs-ICTS were developed for the sensitive detection of thiamethoxam (THI) in vegetables. The limits of detection (LODs) for colorimetric Au@PtNPs-ICTS and catalytic colorimetric Au@PtNPs-ICTS quantitative analysis were 0.18 ng/mL and 0.093 ng/mL, respectively, representing approximately 3-fold and 6-fold improvement compared to AuNPs-ICTS (0.56 ng/mL). Furthermore, highly sensitive detection of multiple pesticide residues (chlorpyrifos, acetamiprid, and imidacloprid) was achieved by replacing the corresponding target antigens and antibodies, which further verified the universality of this immunochromatographic strategy. Fig. 1. (A) Schematic of the preparation process of AuNPs and Au@PtNPs; (B) Schematic of the preparation process of Au@PtNPs-mAb and AuNPs-mAb probes; (C) Schematic diagram of ICTS structure; (D) Schematic diagram of the detection process of Au@PtNPs-ICTS. Download: Download high-res image (151KB) Download: Download full-size image
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高影响因子
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