ANALYST
·
2018
·Vol. 143
·期 10
·2369-2376
·
IF 3.864
Fan Hu,PinPing Wu,RuYa Wang,Wei Liu,Hua He
Herein, a simple, novel, and rapid colorimetric sensor for cimetidine (Cim) detection based on D-xylose protected gold nanoparticles (D-x@AuNPs) has been developed for the first time. The D-x@AuNPs were characterized by UV-vis, TEM and FT-IR techniques. Cimetidine causes the aggregation of D-x@AuNPs due to the formation of a strong covalent Au–N bond and electrostatic binding. As the cimetidine concentration increased, the color of the solutions gradually changed from wine-red to blue, and the large absorption band shifted from 533 to 680 nm upon D-x@AuNP aggregation. The effects of different experimental parameters were investigated. A comparative study on the detection of Cim using citrated capped AuNPs and D-x@AuNPs was presented. Under optimum conditions, the UV-vis spectra showed that the absorption ratio (A680/A533) increased linearly with the concentration of cimetidine in the range of 7 × 10−8 to 3 × 10−6 M with a correlation coefficient of 0.9956 and a limit of detection of 1 × 10−8 M without needing any complicated instruments. The selectivity of the D-x@AuNP detection system for cimetidine was excellent when compared with other ions and analytes. Due to their rapid and visible color changes, and their remarkable selectivity, the D-x@AuNPs synthesized in this study were suitable and could be applied to the detection of cimetidine in human urine.
研究领域:
分析化学
·
纳米技术
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Fang Gong,Rui Peng,Qing Wu,Hean Zhang,Yufeng Luo,Qianling Cui
Surface ligand plays a crucial role in regulating the physicochemical properties and functionalities of gold nanoclusters (AuNCs). Compared to the widely studied thiolate-stabilized AuNCs, the reports on imidazole-based ligands to protect AuNCs are still rare. Herein, we report the facile preparation of imidazole-stabilized AuNCs using 1-vinylimidazole as both a reducing and capping agent. The coordination pattern of imidazole ring to Au core was proved by formation of Au-N bond, which can protect the AuNCs with moderate stability against different pH and temperature. Owing to the weaker binding strength of Au-N bond than Au-S bond, the obtained AuNCs possessed thiol depletion capacity due to ligand exchange in the presence of thiol compounds. By consuming the cellular bio-thiols that act as the reducing species in redox balance, the imidazole-protected AuNCs can cause the accumulation of oxidizing species and the elevation of the oxidative stress . Accordingly, the obtained AuNCs exhibited intrinsic antibacterial activity toward Staphylococcus aureus , which could be a promising bactericidal nanomaterial for sterilization.
研究领域:
纳米技术
·
材料科学
·
细菌学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 2
·1-8
·
IF 5.833
Yin Lixiu,Wang Yueju,Tan Rong,Li Huiling,Tu Yifeng
In order to effectively and conveniently detect the β-amyloid oligomer (AβO) for earlier diagnosis of Alzheimer’s disease (AD), a disposable aptamer biosensor has been developed with high performance, facile operation, and low cost. Using a nanocomposite by in situ reduction of chloroauric acid to decorate Au nanoparticles (AuNPs) on Fe-MIL-88NH 2 material via Au–N bond to effectively enhance the electrochemiluminescence (ECL) of luminol, the functioned basal electrode provides adequate background for sensing response. When the aptamer is linked via Au–S bond on the surface, the sensor gets the ability of specific recognition and coalescence toward the target (AβO). After incubating the sample on the aptasensor, its ECL signal is inhibited owing to the steric hindrance of the AβO macromolecules. The relative inhibition ratio linearly depends to the logarithm of AβO concentration in the range 0.1 pM to 10 pM, with an LOD of 71 fM. The aptasensor has high selectivity to AβO among its analogs. The recoveries in human serum were 98.9–105.4%. This research provides a new approach for sensitive detection of AβO in clinic laboratories for investigation and diagnosis of AD. Graphical abstract
研究领域:
生物医学工程
·
分析化学
·
纳米技术
·
诊断学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2019
·
IF 2.584
Ya Yang,Suyan Zhai,Chao Liu,Xiaoshu Wang,Yifeng Tu
An ultrasensitive disposable immunosensor based on a gold nanoparticles (AuNPs)/indium tin oxide (ITO) substrate has been constructed to detect ketamine (KET) conveniently. In this study, we obtained a AuNPs/ITO basic electrode by only a two-step ecofriendly drip-coating procedure. The self-assembled monolayers of AuNPs were used as a hetero-bifunctional cross-linker and activator, respectively. Ketamine antibody was used as a biorecognition determinant and immobilized onto the AuNPs/ITO through electrostatic interaction or Au–S or Au–N bonds. The architectures of AuNPs/ITO were characterized by transmission electron microscopy, scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy mainly. Electrochemiluminescence (ECL) was used to monitor the specific immune response between the antigen and antibody by measuring changes of intensity in the process of ECL. When the immune complex was incubated on the electrode, it blocked the electron communication and mass transfer, and the ECL signal was quenched. According to the quenched ECL signal, the quantification of KET can be realized. Under optimal conditions, the immunoassay showed a linear range from 10 to 100 pg/g. The low detection limit is 5.73 pg/g. The sensor has been applied to detect ketamine in human hair successfully. In addition, the immunosensor has excellent selectivity, outstanding reproducibility, and acceptable stability. What’s more, the immunosensor exhibits low-cost, simplicity, small sample consumption, and easy miniaturization, which allows it to be integrated into portable platforms to be utilized on the site of crime easily.
研究领域:
分析化学
·
纳米技术
·
法医学
·
生物传感器
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）