Biosensors-Basel
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2021
·Vol. 11
·期 6
·174
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IF 5.519
Fanfan Xiao,Yuting Yu,Yang Wu,Lili Tian,Guoyan Zhao,Hailong Pang,Jie Du
In this paper, we propose a simple colorimetric method for the sensitive and selective detection of alkaline phosphatase (ALP) activity based on the turn off/turn on oxidase mimic activity of[email protected][email protected]/graphene oxide hybrids ([email protected]/GO) were easily obtained using the self-assembly method with poly (diallyldimethylammonium chloride) (PDDA)-coated GO and showed high oxidase-like activity compared with[email protected]We found that the pyrophosphate ion (P2O74−, PPi) could effectively inhibit the oxidase mimic activity of[email protected]/GO, and the hydrolysis of PPi by ALP restored the inhibited activity of[email protected]/GO, enabling them to efficiently catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to generate the blue oxidized product oxTMB. The intensity of the color showed a linear dependency with the ALP activity. ALP was detected in the linear range of 0–40 mU/mL with a low detection limit (LOD) of 0.26 mU/mL (S/N = 3). The proposed method is fast, easy, and can be applied to monitor the ALP activity in serum samples accurately and effectively, which suggests its practicability and reliability in the detection of ALP activity in clinical practice.Keywords:[email protected]/GO;oxidase-like activity;PPi;ALP;colorimetric detection
研究领域:
分析化学
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生物化学
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纳米技术
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Xiaoling Sun,Zhizhuo Gu,YiFan Gao,Maosheng Liang,Lian Xia,Fengli Qu
Luminescent thermometry affords remote measurement of temperature and shows huge potential in future technology beyond those possible with traditional methods. Strategies of temperature measurement aiming to increase thermal sensitivity in a wide temperature response window would represent a pivotal step forward, but most thermometers cannot do both of them. Herein, we propose a balancing strategy to achieve a trade-off between high Arrhenius activation energy (Ea), which could stretch the temperature response windows, and fluorescence quantum yields (QYs) in a manner that will increase thermal sensitivity in a wide response window. In particular, a luminescent thermometer composed of AuNCs-MOF is prepared via a facile impregnation process to enhance QYs and Ea, responsible for high relative sensitivity (Sr) (15.6% K–1) and ultrawide temperature linearity range (from 83 to 343 K), respectively. Taking fluorescence intensity and lifetime as multiple parameters, the maximum Sr can reach 22.4% K–1 by multiple linear regression. The maximum Sr and temperature response range of the proposed thermometer outperform those of the most recent luminescent thermometers. The strategy of balancing Sr and thermal response range by regulating Ea and QYs enables the construction of ultra-accurate thermal sensors in the age of artificial intelligence.
研究领域:
材料科学
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光学工程
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化学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
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2022
·Vol. 10
·期 27
·5182-5190
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IF 7.571
Ze Wang,Liang He,Songtian Che,Huiyuan Xing,Lin Guan,Zhe Yang,Xingchen Li,Andrei V. Zvyagin,Quan Lin,Wenrui Qu
As the most common cancer in men worldwide, prostate cancer has a serious impact on people's health. Until now, the development of a platform for integrating tumor targeting, imaging and an effective treatment for prostate cancer has remained challenging. Herein, a nano-system is designed to improve both diagnosis and treatment for prostate cancer. We successfully synthesized an AuNCs–LHRHa nano-system by combining PEI-modified gold nanoclusters (AuNCs) with LHRH analogues (LHRHa). Due to the good tunable optical properties and photothermal properties of AuNCs, the nano-system can not only achieve efficient fluorescence/computed tomography dual-mode imaging, but can also be used for photothermal therapy (PTT). After modifying the LHRHa antibody of a prostate tumor, AuNCs–LHRHa can be more effectively recognized by the gonadotropin-releasing hormone receptors (GnRH-R) on the membrane of RM-1 cells, enhancing the tumor cell uptake of the nano-system, improving the targeting accuracy and PTT therapy efficacy for prostate cancer. It is hoped that the nano-system, which combines dual-mode imaging and targeted therapy, will provide a promising strategy for the integration of FL/CT diagnosis and PTT therapy for GnRH-R positive prostate cancer.
研究领域:
癌症研究
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纳米技术
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医学成像
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诊疗一体化
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生物医学工程
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2020
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IF 3.594
Wanying Fu,Hongying Wang,Yanwei Chen,Jinglei Ding,Guiye Shan
In this work, a gold nanocluster with lysine (Lys) as a ligand was fabricated, and a novel nanocomposite probe (AuNCs@Lys-MnO 2 ) was prepared based on fluorescence resonance energy transfer (FRET). AuNCs@Lys-MnO 2 was manufactured for sensitive and rapid sensing of glutathione (GSH) in human serum. Gold nanoclusters (AuNCs@Lys) and manganese dioxide nanosheets (MnO 2 nanosheets) are combined by electrostatic interaction. MnO 2 nanosheets can quickly quench the fluorescence of AuNCs@Lys through FRET. GSH has strong reducing ability, which can reduce MnO 2 and degrade AuNCs@Lys-MnO 2 FRET system. The presence of GSH subsequently releases AuNCs@Lys, which causes partial recovery of fluorescent signal. The nanocomposite probe is successfully applied to detect GSH in human serum. Detection range is near the normal value in human serum, and there is no need to dilute the serum during the detection process. This is a fast, simple, and cost-effective strategy. The method can detect GSH in the concentration range of 0.5 to 1000 μM, and has a very high sensitivity, the detection limit is as low as 5 nM.
研究领域:
分析化学
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纳米技术
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生物传感器
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生物医学工程
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DOI
高影响因子
ANALYST
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2021
·Vol. 146
·期 16
·5115-5123
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IF 4.616
Haiyang Yao,Difei Jiang,Gaoqiu Dong,Jiamin Sun,Shasha Sun,Lingling Li,Fenfen Zheng,Weiwei Xiong
Dynamically monitoring intracellular glutathione (GSH), a crucial biomarker of oxidative stress, is of significance for the diagnosis and treatment of certain diseases. Although manganese dioxide (MnO2) based GSH fluorescent sensors have exhibited high sensitivity and good selectivity owing to the specific reactivity between GSH and MnO2, near-infrared (NIR) MnO2 based nanoprobes for GSH detection are scarce. Herein, we have developed a NIR activatable fluorescence nanoprobe for the imaging and determination of intracellular GSH based on a core–shell nanoparticle, consisting of NIR emitted gold nanocluster doped silica as the fluorescent core and manganese dioxide as the GSH-responsive shell (named AuNCs@MnO2). Due to the absorption competition mechanism, the outer MnO2 shell rather than the inner AuNCs core preferentially absorbed the excitation light, thus leading to fluorescence quenching of the inner AuNCs core. Upon addition of GSH, the fluorescence of the nanoprobe restored along with the reduction of MnO2 to Mn2+ because of the absorption competition disappearance-induced emission. The activatable fluorescence linearly increased upon changing the GSH concentration in the range of 2 to 5000 μM with a detection limit of 0.67 μM. The cytotoxicity test shows that the AuNCs@MnO2 nanoprobes have a good biocompatibility. After entering the cancer cells, the intracellular GSH degraded the outermost MnO2 shell and initiated the NIR fluorescence restoration of AuNCs, which can be used to monitor the dynamic change of intracellular GSH. This strategy provides an NIR-activatable way to detect GSH levels in living cells and offers a promising platform for the diagnosis and treatment of GSH-related diseases.
研究领域:
生物医学工程
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纳米技术
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分析化学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FLUORESCENCE
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2023
·
IF 2.7
Xu Zichen,Xu Hedan,Duan Hongliang,Li Junjie,Hu Xiao,Jiang Kaixin,Wang Guixue,Zhang Yuanyuan
Simple and sensitive detection of cardiac biomarkers is of great significance for early diagnosis and prevention of acute myocardial infarction (AMI). Here, a ratiometric fluorescent nanohybrids probe (AuNCs-QDs) was synthesized through the coupling of bovine serum albumin-functionalized gold nanoclusters (AuNCs) with CdSe/ZnS quantum dots (QDs) to realize simple and sensitive detection of cardiac biomarker myoglobin (Mb). The AuNCs-QDs probe shows pink fluorescence under UV light, with two emission peaks at 468 nm and 630 nm belonging to QDs and AuNCs, respectively. Importantly, the presence of Mb caused fluorescence quenching of the blue-emitting QDs, thereby inhibiting the fluorescence resonance energy transfer (FRET) process between QDs and AuNCs, and reducing the fluorescence intensity ratio (F 468 /F 630 ) of AuNCs-QDs probe effectively. As the concentration of Mb increases, the ratiometric fluorescent probe also exhibits a visible fluorescence color change. The detection limit was as low as 4.99 μg/mL, and the response of the probe to Mb showed a good linear relationship up to 0.52 mg/mL. Moreover, the probe has excellent specificity for Mb. Besides, the AuNCs-QDs has been applied to detect Mb of urine samples. More importantly, we also developed an AuNCs-QDs probe modified smartphone-aided paper-based strip for on-site monitoring of Mb. As far as we know, this is the first report of a smartphone-aided paper-based strip for on-site quick monitoring of Mb, which provides a useful approach for AMI biomarker monitoring and may can be extended to other medical diagnostics.
研究领域:
生物传感器
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纳米技术
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医学诊断
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心脏病学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2018
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IF 6.042
Zhichao Liu,Shengnan Wang,Wanying Li,Yang Tian
Iron ions, as a main component of intracellular labile iron, not only play an important function in oxygen transport, enzymatic reactions, and electron transport but also are vitally important in oxidative stress. In this work, we developed a ratiometric fluorescent biosensor for ferrous ion (Fe2+), in which gold nanoclusters (AuNCs) were synthesized as a stable fluorescent probe and a ligand (FeL) was designed for specific recognition of Fe2+ and conjugated onto AuNCs (AuNC@FeL). Meanwhile, water-soluble sulfocyanine 7 N-hydroxysuccinimide ester (Cy7 NHS ester) was immobilized onto AuNC@FeL as a reference element. The developed ratiometric fluorescent nansosensor displayed good linearity with the concentration of Fe2+ in the range of 1–105 μM, and detection limit was achieved down to 210 nM. In addition, this nanosensor responded to Fe2+ in less than 1.23 s and showed high selectivity against other metal ions, amino acids, and reactive oxygen species. With the advantages of high selectivity and accuracy, as well as quick response and long-term stability, this organic–inorganic ratiometric fluorescent probe was successfully applied in real-time biosensing and bioimaging of Fe2+ in neurons and HepG2 cells. By use of this tool, it was found that the increasing concentration of Fe2+ in live cells was closely related to oxidative stress.
研究领域:
生物成像
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细胞生物学
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分析化学
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DOI
PubMed
高影响因子
TALANTA
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2022
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IF 6.556
Qi Dai,Zichun Qi,Zhenzhen Yan,Biao Yu,Jingqiu Li,Baosheng Ge,Hua He,Fang Huang,Xiaojuan Wang
A novel ratiometric fluorescent tyrosinase assay is developed based on hybrid nano-assembly of gold nanocluster and tyrosine-containing peptides. The AuNCs@YCY nano-probe ( AYNP ) is fabricated through the hydrophobic interactions and π-π stacking between the tyrosine residues of the Tyr-Cys-Tyr tripeptide (YCY) and the ligands on the surfaces of AuNCs under the near-isoelectric pH value. The resulted AYNP shows distinct fluorescence responses, spontaneous turn-on of the blue emission and turn-off of the near-infrared emission, with a single wavelength excitation. It is demonstrated that the enhancement and quenching are due to the production of pheomelanin and dopaquinone structures, respectively, induced by tyrosinase oxidation. The internal referencing system provides the tyrosinase assay with superior sensitivity and a detection limit as low as 6.3 U L −1 could be achieved. The experimental results also demonstrate the excellent selectivity, good photo-stability, and both in vitro and cellular applications of AYNP . This assay technique is low-cost, easy to prepare, and shows excellent potential as a novel melanoma clinical diagnostic platform and a tyrosinase inhibitor screening tool.
研究领域:
生物化学
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医学诊断
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药物筛选
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2017
·Vol. 41
·期 11
·4416-4423
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IF 3.269
Hui Yu,Yan Liu,Jiamin Wang,Qing Liang,Hong Liu,Jian Xu,Shijun Shao
A novel organic–inorganic ratiometric fluorescent probe AuNCs–NBD was developed for the detection of cysteine (Cys) and homocysteine (Hcy). In our design, the AuNCs–NBD exhibited dual-emission fluorescence properties with a single excitation. Bovine serum albumin (BSA) protected gold nanoclusters (AuNCs) with carboxyl groups were employed as a reference signal unit and conjugated with the p-aminothiophenol substituted NBD (7-nitro-2,1,3-benzoxadiazole) moiety to form the ratiometric fluorescent probe. The AuNCs–NBD composite showed a single red fluorescence at 630 nm ascribed to AuNCs, and reaction of AuNCs–NBD with Cys and Hcy caused the cleavage of the thioether bond and therefore generated the fluorescent amino linked NBD with emission at 540 nm. Accordingly, the green fluorescence of released NBD at 540 nm gradually increased upon increasing the concentration of Cys or Hcy, while the red fluorescence ascribed to AuNCs at 630 nm remained constant, which resulted in the ratiometric determination of Cys and Hcy using the AuNCs–NBD probe. Due to the specific recognition of NBD with Cys and Hcy, the AuNCs–NBD probe exhibited high selectivity for Cys and Hcy over other amino acids and GSH. In addition, the AuNCs–NBD composite displayed desirable properties such as high specificity, appreciable solubility, ratiometric fluorescence response and favorable detection limit. Moreover, the AuNCs–NBD probe has been successfully applied for monitoring and imaging of intracellular Cys and Hcy in HeLa cells.
研究领域:
分析化学
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生物医学
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细胞生物学
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Shanshan Wang,Yingyi Wang,Yi Peng,Xiaoming Yang
Integrating kinds of bactericides as one entirety shows prospect for improving antibacterial efficiency, thus a new type of nanoclusters based on bactericide-directed herein emerged as a combination for achieving a higher antibacterial effect. Specifically, an easily operated approach for preparing bacitracin-directed silver, gold, and copper nanoclusters (AgNCs, AuNCs, and CuNCs, respectively) was proposed. Meanwhile, AgNCs@Bacitracin, AuNCs@Bacitracin, and CuNCs@Bacitracin emitted cyan, yellow, and red colors of fluorescence, respectively, and different sizes and nitrogen contents of these nanoclusters played a critical role for their fluorescence alterations. Significantly, AgNCs@Bacitracin exhibited robust bacteria-killing efficiency than other nanoclusters, owing to its marked damage on the bacterial membrane. Additionally, propidium iodide (PI) staining indicated the bacterial membrane damage of 72.3% to the bacteria population followed by the treatment with AgNCs@Bacitracin, and the introduction of AgNCs@Bacitracin lead to the higher reactive oxygen species (ROS) production, facilitating their attractive antibacterial activity. Moreover, this strategy of developing new nanoclusters broadened the avenues for designing the improved antibacterial materials.
研究领域:
纳米技术
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材料科学
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微生物学
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DOI
PubMed
高影响因子
Advances in Materials Science and Engineering
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2021
·
IF 1.726
Yi Shi,Xianhui Gao,Wei Wei,Youguo Chen
Here, we reported a simple method for recognizing CA125 overexpressed serum samples using array-based fluorescent gold nanoclusters (AuNCs). The sensing array was fabricated by the combination of various CA125 aptamer functionalized AuNCs. The fluorescence of different aptamer-capped AuNCs was quenched to a different extent in the presence of CA125. By comprehensive analysis of these fluorescence changes, unique patterns were formed when CA125 was overexpressed in serum samples. This strategy is successfully used for discriminating CA125 overexpressed human serum samples, which have great importance for the diagnosis of ovarian cancer in the early stage.
研究领域:
纳米技术
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癌症诊断
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生物传感器
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分析化学
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DOI
高影响因子
BioNanoScience
·
2025
·Vol. 15
·期 2
·1-10
·
IF 3.2
Cai He-Qing,Han Lu,Xue Xin-Yu,Zhang Kou,Wang Tian-Hao,Pan Fu-Hong,Sun Zhi-Cheng,Liu Ru-Ping,Hu Kun,Wei Yen
Gold nanocages (AuNCs) exhibit tunable local surface plasmon resonance (LSPR) properties and exceptional drug loading and releasing capabilities, attributes stemming from their hollow interiors and ultra-thin porous walls. These features underscore their promising applications in the biomedical field. In this study, we fabricated AuNCs with LSPR peaks at 810 nm via chemical etching of Ag nanocube templates with sharp edges using a precisely controlled volume of chloroauric acid. To impart gene-loading functionality, we chemically anchored thiol-modified polyethyleneimine (PEI) onto the surfaces of AuNCs through robust Au–S bonds, generating a novel gene carrier, AuNCs-PEI. Subsequently, we evaluated its photothermal efficacy using a femtosecond laser and assessed cytotoxicity via MTT assay. The binding affinity of AuNCs-PEI for EGFP plasmids was characterized by gel retardation assays. Furthermore, the gene delivery efficiency of AuNCs-PEI was examined in 293 T cells by quantifying green fluorescence protein signals using inverted fluorescence microscopy. These findings demonstrate that engineered AuNCs-PEI not only facilitates gene loading for therapeutic applications but also enables photothermal therapy (PTT).
研究领域:
生物医学工程
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纳米医学
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基因治疗
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）