MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 6
·1-16
·
IF 5.3
Li Guiyin,Guo Fei,Liang Jianlu,Wan Bingbing,Liang Jintao,Zhou Zhide
A new sandwich-type electrochemical biosensing platform was developed by gold @polyphthalenediamine nanohybrids (AuNP@PoPD) as the sensing platform and phosphorus doped reduced graphene oxide-hemin-palladium nanoparticles (PrGO-Hemin-PdNP) as the signal amplifier for phosphatidylinositol proteoglycan 3 (GPC3). AuNP@PoPD, co-electrodeposited into the screen printed electrode with high conductivity and stability, is dedicated to assembling the primary GPC3 aptamer (GPC3 Apt ). The second GPC3 Apt immobilized on the high conductivity and large surface area of PrGO-Hemin-PdNP was utilized as an electrochemical signal reporter by hemin oxidation (PrGO-Hemin-PdNP-GPC3 Apt ). In the range 0.001–10.0 ng/mL, the hemin oxidation current signal of the electrochemical aptasensor increased log-linearly with the concentration of GPC3, the lowest detection limit was 0.13 pg/mL, and the sensitivity was 2.073 μA/μM/cm 2 . The aptasensor exhibited good sensing performance in a human serum sample with the relative error of 4.31–8.07%. The sandwich sensor showed good selectivity and stability for detection GPC3 in human serum samples, providing a new efficient and sensitive method for detecting HCC markers. Graphical Abstract
研究领域:
生物医学工程
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分析化学
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肿瘤学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 37
·22744-22750
·
IF 3.361
Qi Qu,Jing Wang,Chuan Zeng,Mengfan Wang,Wei Qi,Zhimin He
Owing to the high sensitivity, fast responsiveness and high specificity, immunoassays using surface-enhanced Raman scattering (SERS) as the readout signal displayed great potential in disease diagnosis. In this study, we developed a SERS-immunoassay method for the detection of human immunoglobulin G (HIgG). Upon involving well-ordered AuA on a SERSIA substrate, the LSPR effect was further enhanced to generate a strong and uniform Raman signal through the formation of sandwich structure with the addition of target HIgG and SERSIA tag. Optimization of the assay provided a wide linear range (0.1–200 μg mL−1) and low limit of detection (0.1 μg mL−1). In addition, the SERS-immunoassay method displayed excellent specificity and was homogeneous, which guaranteed the practical use of this method in the quantitative detection of HIgG. To validate this assay, human serum was analysed, which demonstrated the potential advantages of SERS-immunoassay technology in clinical diagnostics.
研究领域:
生物医学工程
·
分析化学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Zhilei Zhao,Mengyu Ren,Wenqi Zhang,Zikuo Chen,Lu Zhang,Xinquan Qu,Junling Shi,Weiwei Xia,Xiaoguang Xu,Ying Yang
In view of the serious harm and widespread pollution caused by deoxynivalenol (DON), it is crucial to quantify its presence in the food safety assessment process. Here, we designed a core-shell-satellite nano assembly structure as a probe, composed of an anisotropic AuNR@Ag core, an ultra-thin SiO 2 layer and a high surface coverage AuNPs satellites, which showed outstanding SERS activity and high stability. Anti-DON antibodies were modified on the surface of the prepared core-shell-satellite nano-assembly structure as SERS immunoprobe for DON specific detection using SERS-based lateral flow immunoassay (LFIA) with the advantages of simplicity, rapidity, and high sensitivity. Under optimal conditions, the detection limit for detecting DON using the SERS-LFIA method was 0.053 fg/mL, and the linear detection ranged from 0.1 fg/mL to 1 μg/mL. The SERS-LFIA strips based on AuNR@Ag@SiO 2 -AuNP nanostructures demonstrated the great potential for quick quantitative DON detection, providing a reference for trace detection of highly toxin mycotoxins.
研究领域:
食品科学
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分析化学
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纳米技术
·
毒理学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 2
·1-8
·
IF 5.833
Yin Lixiu,Wang Yueju,Tan Rong,Li Huiling,Tu Yifeng
In order to effectively and conveniently detect the β-amyloid oligomer (AβO) for earlier diagnosis of Alzheimer’s disease (AD), a disposable aptamer biosensor has been developed with high performance, facile operation, and low cost. Using a nanocomposite by in situ reduction of chloroauric acid to decorate Au nanoparticles (AuNPs) on Fe-MIL-88NH 2 material via Au–N bond to effectively enhance the electrochemiluminescence (ECL) of luminol, the functioned basal electrode provides adequate background for sensing response. When the aptamer is linked via Au–S bond on the surface, the sensor gets the ability of specific recognition and coalescence toward the target (AβO). After incubating the sample on the aptasensor, its ECL signal is inhibited owing to the steric hindrance of the AβO macromolecules. The relative inhibition ratio linearly depends to the logarithm of AβO concentration in the range 0.1 pM to 10 pM, with an LOD of 71 fM. The aptasensor has high selectivity to AβO among its analogs. The recoveries in human serum were 98.9–105.4%. This research provides a new approach for sensitive detection of AβO in clinic laboratories for investigation and diagnosis of AD. Graphical abstract
研究领域:
生物医学工程
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分析化学
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纳米技术
·
诊断学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Chang Liu,Siyu Gou,Yanhui Bi,Qi Gao,Juanjuan Sun,Shanjin Hu,Weiwei Guo
A portable, cost-effective and storable DNA-gold nanoparticle (AuNP) hybrid hydrogel film based biosensing system was developed, with AuNPs serving as both the crosslinking units of the film and the signaling units. Using a layer-by-layer assembly method, hydrogel film composed of three-dimensional hydrophilic network of densely packed AuNPs interconnected by responsive DNA structures was constructed onto a glass slide. By programming the sequence of DNA structures, target-responsive hybrid films were constructed. As a proof of concept , the sequence of a substrate DNA which can be identified and cleaved by Pb 2+ -dependent DNAzyme was encoded to construct Pb 2+ -responsive DNA-AuNP hybrid hydrogel film. The high-density packing of AuNPs as signal substances significantly improved the sensitivity of the ultrathin film biosensing system while reduced the cost of expensive DNA materials. A hydrogel film composed of 10 layers of assembled DNA-AuNP structures generated sufficient visual colorimetric signals for Pb 2+ detection, with a detection limit of 2.6 nM. By introducing UO 2 2+ -dependent DNAzyme, the system could be further applied in the sensitive and selective detection of UO 2 2+ , with a detection limit of 10.3 nM. Compared with bulk-sized DNA hydrogel biosensing systems, the DNA-AuNP hydrogel film biosensing system exhibited faster response thanks to the sub-micrometer ultrathin film structures. Moreover, the protection of fragile non-covalently crosslinked DNA films with solid slides also facilitated the portable application and long-term storage of the resulting biosensing system, with 95% of the response signal retained after three months of storage. The DNA-AuNPs hydrogel film biosensing system is highly promising for future rapid on-site detection applications.
研究领域:
生物化学
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纳米生物学
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环境科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Minyi Yang,Rongye Jia,Yuxuan Liu,Hengkun Tang,Huijuan Wu,Jun Yuan,Rongzhi Wang
Furosine, a hazardous compound mainly produced by the Maillard reaction in food at high temperatures, has been shown to exert cytotoxic effects on cells and cause liver damage after excessive consumption. Hence, it is desirable to develop correlative sensitive and robust immunoassays for furosine detection in real-world samples. In this study, Ovalbumin-furosine (OVA-furo) conjugates were employed as the immunogens for animal immunization, and a hybridoma (1C3) secreting an anti-furosine monoclonal antibody (mAb) was successfully screened. The purified 1C3 mAb exhibited high specificity and affinity (7.4 × 10 8 L/mol), and an indirect competitive ELISA (ic-ELISA) based on the 1C3 mAb was developed for the detection of furosine. The linear detection range of the ic-ELISA was 6.03–817.85 ng/mL, with a limit of detection (LOD) value of 2 ng/mL. Colloid gold nanospheres(AuNP-) and nanoflower gold-particles(AuNF)-based strips were also established based on this antibody, which had qLOD values and linear ranges of 40 and 20 ng/mL and 40–140 and 20–90 ng/mL, respectively. All the immunoassays showed good detection performance, indicating that these immunoassays have the potential for practical application for the inexpensive detection of furosine in real-world samples.
研究领域:
食品科学
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免疫学
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产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2019
·Vol. 12
·期 5
·1121-1127
·
IF 2.413
Wu Zhengzong
A novel aptasensor was described and employed for ultrasensitive determination of the antibacterial agent oxytetracycline (OTC). This method was based on gold nanoparticle (AuNP) tetramers and surface-enhanced Raman spectroscopy (SERS). Under optimal conditions, in the range of 0.01–250 ng/mL, the concentration of OTC exhibited good linear relationship ( R 2 = 0.9948) with changed SERS signal intensity. In addition, the applicability of the novel aptasensor was validated by detecting different concentrations of OTC spiked in milk samples. With its satisfactory performances, this aptasensor is perceived to be a promising candidate for determining OTC in food products. This assay could also be extended for the analysis of other targets (pathogens or harmful substances) in food products by using corresponding DNA sequences.
研究领域:
分析化学
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食品安全
·
纳米技术
·
环境监测
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产品关联
DOI
高影响因子
Nanoscale
·
2013
·Vol. 5
·期 17
·8130-8137
·
IF 6.233
Jun Deng,Mingcong Sun,Jiyu Zhu,Changyou Gao
Protein adsorption influences greatly the performance of materials used in biotechnology and biomedicine. The binding of fibrinogen (Fg) to nanoparticles (NPs) can result in protein unfolding and exposure of cryptic epitopes that subsequently interact with cell surface receptors. The response and its degree are dependent on the size, charge, and concentration of the NPs. In this study the binding kinetics of human Fg to negatively charged 11-mercaptoundecanoic acid-functionalized gold nanoparticles (AuNPs–COOH) ranging from 5.6 to 64.5 nm were examined. The larger NPs bound Fg with a larger number of proteins per square unit and a higher dissociation rate (Kd′), but with decreased affinity. By contrast, the 5.6 nm AuNPs–COOH behaved in a cooperative manner for Fg adsorption. In the presence of excess Fg, only the 64.5 nm AuNPs–COOH showed severe aggregation, whose degree was alleviated in a dilute Fg solution. The Fg is adsorbed through a side-on configuration and both side-on and end-on configurations on the smaller (5.6 and 14.2 nm) and 31.5 nm AuNPs–COOH, respectively. It also retains the native conformation. By contrast, on the 64.5 nm AuNPs–COOH the Fg adopts the end-on configuration and loses most of the secondary structure.
研究领域:
生物化学
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纳米医学
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分子生物学
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DOI
高影响因子
ANALYST
·
2019
·Vol. 144
·期 20
·5996-6003
·
IF 4.019
Mei-Ling Gao,Bin-Cheng Yin,Bang-Ce Ye
As exosomes have been playing an increasingly important role in the diagnosis, treatment and prognosis of diseases, the analysis of exosome contents becomes more crucial. Therefore, the development of a cost-effective and simple exosome separation method that achieves high purity is urgently needed, and it is vital for further research in cancer. In this work, we constructed a DNA-AuNP-based satellite network which integrates low-speed centrifugal exosome isolation, detection and protein analysis. The rolling circle amplification (RCA) reaction is used to produce a long-chain DNA hairpin structure comprising a plurality of functional domains, such as CD63 aptamer sequences, linker sequences, and spacer sequences with complementary base pairs to form a hairpin structure. When the CD63 aptamers bind to exosomes, the hairpin structure changes its conformation, exposing the linker sequences (AuNP binding sequence). Then the probe on the surface of AuNPs combines with the long-chain DNA by the toehold-mediated strand displacement reaction, releasing the fluorescent labeled complementary probe as the detection signal and simultaneously forming the DNA-AuNP-based satellite network. Thus, exosomes can be isolated by low-speed centrifugation. The formation of the DNA-AuNP-based satellite network was confirmed by transmission electron microscopy and confocal fluorescence microscopy. In addition, we established a standard curve for exosome detection which showed good linearity of the fluorescence ratio vs. log(exosome concentration). LC/MS for protein profiling of the captured exosomes demonstrated that our method has potential application in the field of exosome research.
研究领域:
生物技术
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纳米技术
·
分子生物学
·
癌症研究
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产品关联
DOI
PubMed
高影响因子
ACS Sensors
·
2020
·
IF 7.333
Chenyun Wang,Cancan Wang,Dan Jin,Yi Yu,Fan Yang,Yulin Zhang,Qunfeng Yao,Guo-Jun Zhang
In this study, we report a gold nanoparticle (AuNP)-amplified surface acoustic wave (SAW) sensor for exosome detection with high sensitivity. The SAW chip was self-assembled with mercapto acetic acid to generate carboxylic groups via the Au–S bond. Anti-CD63 was then anchored onto the chip by pretreatment with 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide,1-hydroxypyrrolidine-2,5-dione (NHS). Due to the existence of a membrane protein, CD63, on the exosome surface, exosomes could be bound onto the antibody-immobilized SAW chip. To amplify the detection signal, both the biotin-conjugated epithelial cell adhesion molecule (EpCAM) antibody as a secondary antibody and AuNP-labeled streptavidin were applied onto the exosome-bound SAW chip, resulting in AuNP assembly on the chip through biotin–avidin recognition. The sensor was capable of detecting 1.1 × 103 particles/mL exosomes, which was about 2 orders of magnitude higher than those detected by the strategy without using signal amplification. The sensor also achieved a satisfactory specificity and could detect the low-abundance exosomes directly in blood samples from cancer patients with minimal disturbance. This makes the SAW sensor useful for early diagnosis of cancer.
研究领域:
生物医学工程
·
纳米技术
·
癌症诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Tissue Engineering and Regenerative Medicine
·
2023
·
IF 3.6
Wang Jie,Zhang Hang,Wang Yan,Liu Xiang,Zhu Weiwen,Jiang Fei,Li Size,Liu Laikui
Background: Guided bone regeneration (GBR) is commonly used to regenerate periodontal tissue. However, the bone inductivity and antibacterial properties of the GBR membranes currently in use are severely limited. This issue can be resolved by loading growth factors and antibiotics. Bioactive substitutes, such as Au nanoparticles (AuNPs) and carbon quantum dots (CDs), were proposed to prevent the denaturation of osteogenic growth factors and the induction of antibacterial drug resistance. Methods: Ornidazole was initially used as the raw material to prepare the CDs, followed by the incorporation of an optimal ratio of nanoparticles to produce the electrospun membrane doped with AuNPs and novel traceable antibacterial CDs. The morphology of the membrane was characterized. The adhesion, proliferation, and osteogenic differentiation of cells on the membrane were evaluated in vitro. The antimicrobial characteristics of the membrane were also investigated. The electrospun membrane was implanted into a rat skull defect model in vivo to investigate its osteogenic potential. Results: The blending of nanomaterials did not affect the micro morphology of the fiber, resulting in enhanced mechanical properties. Membranes doped with AuNPs and CDs exhibited excellent biocompatibility, increased ALP activity, improved calcified nodules, and increased expression of osteogenic-associated proteins, in addition to pronounced antibacterial effects. The membrane also demonstrated excellent osteogenic characteristics in rat models. Conclusion: The synergistic effect of loaded AuNPs electrospun fiber membrane with CDs can promote periodontal bone regeneration and exert antibacterial activity.
研究领域:
牙周组织工程
·
生物材料学
·
再生医学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Guangpei Qi,Haixia Zou,Xiaohong Peng,Shiliang He,Qiqi Zhang,Wei Ye,Yizhou Jiang,Wentao Wang,Guangli Ren,Xiangmeng Qu
Metabolic footprinting as a convenient and non-invasive cell metabolomics strategy relies on monitoring the whole extracellular metabolic process. It covers nutrient consumption and metabolite secretion of in vitro cell culture, which is hindered by low universality owing to pre-treatment of the cell medium and special equipment. Here, we report the design and a variety of applicability, for quantifying extracellular metabolism, of fluorescently labeled single-stranded DNA (ssDNA)-AuNP encoders, whose multi-modal signal response is triggered by extracellular metabolites. We constructed metabolic response profiling of cells by detecting extracellular metabolites in different tumor cells and drug-induced extracellular metabolites. We further assessed the extracellular metabolism differences using a machine learning algorithm. This metabolic response profiling based on the DNA-AuNP encoder strategy is a powerful complement to metabolic footprinting, which significantly applies potential non-invasive identification of tumor cell heterogeneity.
研究领域:
细胞生物学
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纳米技术
·
生物化学
·
癌症研究
·
机器学习
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）