SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Jiaojiao Yang,Lijun Feng,Jing Liu,Sheng Li,Na Li,Xinfeng Zhang
Mercury (Hg) is an important environmental pollutant because of its high toxicity. With the rapid industrialization, on-site monitoring Hg 2+ contamination becomes increasingly important. In this work, a facile colorimetric sensing system based on DNA-mediated charge neutralization of AuNPs as well as smartphone data acquisition was developed for on-site monitoring of Hg 2+ . The designed both-end block dsDNA system (probe DNA and block DNA) can interact with the replacement DNA and formed T-Hg 2+ -T mismatched dsDNA in the presence of Hg 2+ , thus releasing the long block DNA as well as preventing the ThT-induced neutralization of AuNPs. The color change for the AuNPs neutralization can be pictured and analyzed by a portable smartphone. This on-site Hg 2+ monitoring system was quite simple and sensitive with a low limit of detection (2.8 nM), lower than the maximum permitted amount of Hg 2+ in drinking water regulated by EPA. The accuracy of the proposed method was verified through analysis of two certified water samples, four real water samples and seven cosmetic samples, and the results agreed well with the certified values or those by atomic fluorescence spectrometry . Overall, this facile and simple sensing system is an appealing tool for on-site monitoring of Hg 2+ in water and cosmetic samples.
研究领域:
环境科学
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分析化学
·
生物传感
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2024
·
IF 2.7
Xin Hua,Tongxiao Zhao,Xueqin Gui,Baokang Jin
In this paper, a label-free electrochemical immunosensor for sensitive detection of prostate antigen (PSA) was developed based on a NiFe PBA/AuNPs composite. The prostate antigen antibody was immobilized and the immunosensor was constructed by using a glassy carbon electrode modified with a nanocomposite consisting of nickel–iron Prussian blue analog (NiFe PBA) and gold nanoparticles (AuNPs). Due to the good biological affinity of AuNPs for biomolecules, as well as the porous nanostructure and regular shape of NiFe PBA, NiFe PBA/AuNPs nanocomposites significantly improve the electron transport rate, while achieving excellent performance for the sensor. Due to the interaction between the antibody and the antigen on the modified electrode, the current signal of the NiFe PBA itself is reduced due to the redox changes in Fe2+ and Fe3+, which can be determined by differential pulse voltammetry (DPV). Therefore, the monitoring of prostate antigen detection is realized. Under optimal experimental conditions, the immunosensor exhibited excellent detection performance with a dynamic response range from 0.5 pg mL−1 to 1000 pg mL−1 for the PSA concentration and a detection limit of 0.23 pg mL−1 (S/N = 3). In addition, the PSA aptasensor has good selectivity, high stability, and satisfactory reproducibility and has broad potential in clinical research and diagnostic applications.
研究领域:
生物医学工程
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分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2021
·
IF 5.455
Sisi Su,Lanlan Lin,Haiyang Li,Xinyu Wen,Rui Yan,Caihong Tao
Photothermal material was one of the hot topics in nanomedicine in recent years, which had aroused widespread attention of scholars. In this paper, a kind of core-shell drug nanocarriers with good photothermal properties were prepared, and its photothermal therapeutic effect on tumor cells in vitro was studied. First, fluorescein isothiocyanate (FITC) was encapsulated in silica nanoparticles to form silica fluorescent (F-SiO 2 ) nanoparticles. Then mesoporous dopamine (MPDA) layer were built on the F-SiO 2 nanoparticles, the core-shell nanoparticles (F-SiO 2 @MPDA) with mesoporous shell structure were obtained. Finally, Au nanoparticles (AuNPs) were wrapped on the surface of the layer of MPDA shell by the citrate reduction method, and the F-SiO 2 @MPDA-AuNPs composite nanoparticles were formed. Then, the fluorescence property, adsorption performance, sustained release property, photothermal property and cytotoxicity of the F-SiO 2 @MPDA-AuNPs nanoparticles were researched. It was found that F-SiO 2 @MPDA-AuNPs nanoparticles had good fluorescence performance, high adsorption capacity (DOX, 111.275 mg/g) and pH responsive release performance. When pH = 5.2, the DOX release rate was 39.5% within 7 h under the action of near-infrared light (NIR). In addition, it also had a high photothermal conversion rate (36.95%), and the DOX loaded F-SiO 2 @MPDA-AuNPs nanoparticles had obvious inhibitory effect on tumor cells in vitro. As a kind of mesoporous drug nanocarriers with photothermal properties, it has potential application value.
研究领域:
纳米医学
·
生物材料
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肿瘤学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Jiani Zhang,Xiaohui Guo,Jian Zhang,Xiaobing Guo,Yuying Xu,Lijuan Chen
Phoxim, extensively utilized in agriculture as an organothiophosphate insecticide, has the potential to cause neurotoxicity and pose human health hazards. In this study, an electrochemical enzyme biosensor based on Ti 3 C 2 MXene/MoS 2 @AuNPs/AChE was constructed for the sensitive detection of phoxim. The two-dimensional multilayer structure of Ti 3 C 2 MXene provides a robust framework for MoS 2 , leading to an expansion of the specific surface area and effectively preventing re-stacking of Ti 3 C 2 MXene. Additionally, the synergistic effect of self-reduced grown AuNPs with MoS 2 further improves the electrical conductivity of the composites, while the robust framework provides a favorable microenvironment for immobilization of enzyme molecules. Ti 3 C 2 MXene/MoS 2 @AuNPs electrochemical enzyme sensor showed a significant response to phoxim in the range of 1 × 10 −13 M to 1 × 10 −7 M with a detection limit of 5.29 × 10 −15 M. Moreover, the sensor demonstrated excellent repeatability, reproducibility, and stability, thereby showing its promising potential for real sample detection.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2024
·
IF 2.7
Weiqiang Li,Xiaoxue Liu,Pianpian He,Wentao Hu,Kaijie Tang,Yangping Wen,Qian Zeng,Huiyi Tang,Yaxuan Lei,Xin Liu
Deoxynivalenol (DON) has drawn considerable attention for its obvious pathogenicity and wide use in agro-products, which cause a potential threat to human health. In this work, an electrochemical immunosensor is developed for the highly sensitive and selective detection of DON in wheat flour using AuNPs-BP-MWCNTs-COOH and antibodies. The AuNPs-BP-MWCNTs-COOH nanocomposite was prepared via an in situ reduction reaction and ultrasonic-assisted liquid-phase exfoliation. The nanocomposite exhibits a larger surface area, decent stability, excellent electron transfer capability, good protein binding capability and prominent specificity. The plentiful carboxyl group on the nanocomposite can bind to the amino group of the antibody, and AuNPs have an affinity for the sulfhydryl group of the antibody, which makes it feasible for the nanocomposite to load the antibody. The peak currents are plotted against the logarithm of DON concentration from 0.002 to 80 ng mL−1 with a limit of detection (LOD) of 0.5 pg mL−1. This approach establishes an effective label-free immunosensor platform for the detection of DON with high sensitivity and selectivity in various food and agricultural products.
研究领域:
食品安全
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分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBON
·
2019
·
IF 7.466
Yi Yu,QingYi Zhu,Feifan Xiang,Yue Hu,Lijie Zhang,Xiangju Xu,Nannan Liu,Shaoming Huang
AuNPs/SWCNT, a hybrid nanomaterial which is single-walled carbon nanotube (SWCNT) modified with gold nanoparticles (AuNPs), has great application prospects for chemical and biological sensors. For the innate nanogap between tiny AuNPs deposited on sidewall of SWCNT, AuNPs/SWCNT is suitable for the fabrication of simple and effective electrical signal switching devices. Here, we develop a novel strategy of applying AuNPs/SWCNT to fabricate nanogap device for DNA hybridization detection. The detection strategy is based on the target DNA mediating the catalytic activity of the AuNPs by desorbing the probe DNA from AuNPs. The signal transduction depends on the growth of tiny AuNPs which is closely related to the AuNPs catalytic activity. Sensing occurs at the tiny AuNPs growing and connecting into a continuous nanowire along the SWCNT, leading to a conductimetric response. The biosensing device can detect target DNA with the detection limit of 1 pM and the ability to discriminate triple and quintuple base pair mismatches.
研究领域:
生物传感器
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纳米技术
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分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Chemical Engineering Journal Advances
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2022
·
IF 0
Wei Xue,Bo Han,Haojie Ma,Xueyan Hou,Ji-Jiang Wang,Ran Li,Yuqi Zhang
Novel TiO 2 nanotube arrays (TNAs) involved heterojunctions are widely used as photocatalyst for pollutants, while the residues are usually detected by other method. A device which integrates photocatalytic degradation and enhancing detection signals could increase the atom utilization and decrease detection cost. In this work, a novel TNAs/g-C 3 N 4 /AuNPs heterojunction was prepared and used as photocatalyst and SERS substrate simultaneously. Dye of crystal violet was successfully degraded, and the concentration of residue was detected by Raman spectroscopy. There was a good linear correlation between the Raman intensity and the concentration of crystal violet from 10 −10 M to 10 −6 M, and the limit of detection (LoD) was 8.79 × 10 −11 M. Moreover, the prepared heterojunctions were used on wastewater and biological staining detection. In the applications of photocatalytic degradation and detection, the time-dependent SERS intensities demonstrated the photocatalytic degradation achieved more than 95% in 20 min, and almost completed in 25 min, and the heterojunctions showed good recyclability in at least 10 cycles. For SERS property, the original enhancement factor (EF) was calculated to be 7.43 × 10 6 , then the EF descended slightly and remained with less than 90% attenuation after 10 cycles.
研究领域:
环境科学
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材料科学
·
分析化学
✓ 已验证
DOI
RSC Advances
·
2021
·Vol. 11
·期 42
·25976-25982
·
IF 3.361
Fengyi Cao,Changmin Wei,Gangqing Ma,Like Hou,Rencong Zhang,Lin Mei,Qi Qin
Cotton gauze has been used as a wound dressing since the 19th century, and still plays an important role in current clinical therapies. However, the antimicrobial ability of cotton gauze is limited. In this work, gold nanoparticles (AuNPs) were used as photothermal transduction agents to synthesize modified photothermal antimicrobial cotton gauze. The modified cotton gauze was synthesized by immersing and heating the clinical cotton gauze with AuNPs solution. XPS, ICP-OES, FTIR, XRD and SEM characterizations confirmed that AuNPs were successfully decorated on the surface of cotton gauzes. Besides, the mechanical properties, air and water vapour permeability performance of cotton gauze were not changed after modification. Photothermal antimicrobial experiments confirmed that AuNPs modified on the cotton gauze could convert light to heat, inducing rapid temperature increase of the cotton gauze. And the heat could kill microbial cells permeated in the modified cotton gauze, giving it the potential of being used for photothermal antimicrobial therapy.
研究领域:
材料科学
·
生物医学工程
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED POWDER TECHNOLOGY
·
2021
·
IF 4.833
Xinyue Chen,Dan Liu,Sanhu Gou,Jiahui Ji,Zhiyuan Xue,Shilan Feng
A colorimetric assay based on the oriented TA-AuNPs-PEG-FITC for rapid and sensitive PABA detection was proposed. Initially, the negatively charged TA modified on the surface of AuNPs enabled rapid binding to PABA due to the electrostatic interaction and hydrogen bond, resulting in a visible color change. However, particles were aggregated in a non-oriented way, leading to an unstable testing system which failed to realize the accurate quantification. Thus, the asymmetrically functionalized TA-AuNPs-PEG-FITC were prepared, in which the stabilizer agent of HS-PEG-FITC was attached to the specific sites on the surface of TA-AuNPs. Such a way of modification resulted in an oriented aggregation manner, the addition of PABA induced the formation of oligomers. Moreover, the introduction of FITC group acted as fluorescent marker, providing a simple, fast and quantitative method for characterization of PEG chain. The molar ratio between TA-AuNPs and modified PEG-FITC was further determined. A linear regression was established in a wide range from 10 μM to 1 mM with the LOD of 6.9 μM. Finally, the sensor was successfully applied in the health food. This was the first example in which TA-AuNPs-PEG-FITC were fabricated for colorimetric detection of PABA, and an indirect method based on fluorescence marker was well used to quantify the content of PEG chain.
研究领域:
分析化学
·
纳米生物学
·
食品科学
✓ 已验证
DOI
高影响因子
Advanced Science
·
2025
·
IF 14.1
Xinyu Xu,Hao Wang,Xiaohan Mei,Dapeng Zeng,Zehao Yu,Shibo Liu,Ruiyan Li,Yanguo Qin
Malignant bone tumor defects are difficult to treat because of the simultaneous need for tumor treatment and bone-repair promotion. This study presents a bioactive composite scaffold (T-rGO@Au) for personalized bone defect repair and bone tumor treatment. The T-rGO@Au scaffold has a porous structure, and its mechanical properties are close to those of human cancellous bone. The T-rGO@Au scaffold can induce upregulation of osteopontin (OPN), RUNX-2, and osteocalcin (OCN) gene expression. In vivo experiments showed that the bone volume/total volume (BV/TV) ratio with the T-rGO@Au scaffold was the highest. The new bone was tightly integrated with the implant, demonstrating effective osseointegration. The T-rGO@Au scaffold locally generated high temperatures and reactive oxygen species under near-infrared excitation, and AuNPs enhanced the photothermal performance of rGO through the “antenna effect.” Furthermore, in vitro experiments showed that the tumor cell nuclei were destroyed, late-stage apoptotic cells increased, and cell morphology was severely damaged. Additionally, RNA-seq revealed that tumor cell destruction was mediated through signaling pathways, such as the MAPK pathway. In vivo antitumor experiments also demonstrated that the T-rGO@Au scaffold significantly inhibited the growth of tumor cells within 2 weeks. Thus, the T-rGO@Au scaffold provides a new treatment strategy for the development of implantable scaffolds for bone tumor defects.
研究领域:
生物医学工程
·
材料科学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Haotian Wu,Gang Zhang,Xiaozhan Yang
An electrochemical biosensor based on iron tetroxide (Fe 3 O 4 )/carboxylated carbon nanotubes (MWCNTs-COOH)/gold nanoparticles (AuNPs) was designed for the detection of alpha-fetoprotein (AFP), which is often used as an important indicator for the early clinical diagnosis of liver cancer markers. The Fe 3 O 4 /MWCNTs-COOH nanocomposite was synthesized by a solvothermal method and it combined with gold nanoparticles (AuNPs) deposited at the constant potential on a glassy carbon electrode to form Fe 3 O 4 /MWCNTs-COOH/AuNPs, which intensifies the electrical signal while the large active sites enable more stable immobilization of the AFP monoclonal antibodies on the electrode. The electrochemical performance of Fe 3 O 4 /MWCNTs-COOH/AuNPs was investigated in detail and the electrochemical response signal after the immune reaction with the AFP antigen-antibody was recorded. The peak current Ip of the response signal is linearly proportional to the lg c AFP in the range of 1 pg mL −1 –10 μg mL −1 , with a detection limit of 1.09034 pg mL −1 and good performance in clinical sample testing. The proposed sensor has shown great application and development potential in clinical medicine field.
研究领域:
生物化学
·
纳米技术
·
生物医学工程
·
临床医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2024
·
IF 5.8
Jiayi Zhu,Chao Zhao,Binyun Xia,Ning Wang,Xi Chen,Xinyue Jing,Minxuan Chen,Xinrui Xu
Abnormal human immunoglobulin G (IgG) may induce the risk of immune system disorder, infectious diseases, tumors and so on. However, the current detection methods exhibit low sensitivity, which limits their practical application. In this work, an SPR optical fiber sensor (SPR-OFS) with high sensitivity is designed for label-free detection of human IgG. It is fabricated using a heterostructure optical fiber coated with Au film/AuNPs and the Ti3C2Tx MXene biofunctionalized with goat anti-human IgG by polydopamine (PDA). In the experiment, the optimal thickness of the Ti3C2Tx MXene was explored and determined to be about 93 nm by comprehensively considering the refractive index (RI) sensitivity and spectral bandwidth of the SPR sensor. When the largest figure of merit (FOM) is calculated to be 17.8279 RIU−1, its RI sensitivity was ultimately found to be 2804.5 nm per RIU. The SPR-OFS was employed to detect human IgG within the concentration range of 0–30 μg mL−1 and its sensitivity is demonstrated to be 1.7046 nm (μg mL−1)−1. The SPR-OFS was also proved to have excellent linearity, specificity and stability. The proposed sensor offers outstanding performance with simple fabrication, providing a cutting-edge bioanalytical platform with potential applications in clinical diagnosis.
研究领域:
生物传感技术
·
医学诊断
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）