PLANT CELL REPORTS
·
2025
·Vol. 44
·期 2
·1-15
·
IF 4.5
Li Xue,Zhou Yue,Chen Hang,Guo Zetian,Zhang Jinlian,Chen Wenli
Key message The three SDEs of C Las were expressed in citrus leaves by AuNPs-PEI mediated transient expression system, and promoted the proliferation of C Las and inhibited citrus immunity. Huanglongbing (HLB) is the most severe bacterial disease of citrus caused by Candidatus Liberibacter asiaticus ( C Las). C Las suppress host immune responses and promote infection by sec-dependent effectors (SDEs), thus insight into HLB pathogenesis is urgently needed to develop effective management strategies. In this study, we focused on the roles of SDE4310 , SDE4435 and SDE4955 in citrus. We found that the expression of SDE4310 , SDE4435 and SDE4955 to increase with increasing citrus immune genes CsPR1 , CsPR2 , CsPR5 , CsNPR1 , CsRBOHD , CsMAP3K and CsBIK1 , suggesting that the level of citrus immunity could be judged by the expression of SDE. To further explore the relationship between these three SDEs and citrus immunity, we established a transient expression system in citrus leaves, using gold nanoparticle-polyethyleneimine (AuNPs-PEI) to deliver recombinant plasmid containing SDE4310 , SDE4435 or SDE4955 respectively into citrus leaves. Results showed that SDE4310 , SDE4435 and SDE4955 were successfully expressed in citrus leaves using this transient expression system, and found that SDE4310 , SDE4435 and SDE4955 could promote the C Las proliferation by decreasing the immune gene expression of the citrus. Additionally, we used AuNPs-PEI to deliver siRNA 4310 to citrus cells, significantly reducing the expression of SDE4310 within 3 days. Although the suppression of SDE4310 expression did not inhibit the C Las proliferation, it increased the expression level of CsPR1 , CsNPR1 and CsBIK1 . This is also the first time that AuNPs-PEI has been found to be able to deliver exogenous plasmids into citrus cells and express the target protein, providing a new method for future studies on citrus HLB.
研究领域:
植物病理学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
ChemNanoMat
·
2020
·Vol. 6
·期 5
·765-772
·
IF 3.384
Di Zhang,Qi Wang,Xiaocui Cheng,Huanhuan Li,Qiuyue Wang,Fangfang Yu,Wenying Wang,Jing Xin,Qingmei Li,Changfeng Dou,Xiaoqing Wu,Hongli Qian,Wei Fang,Fengrui Wu,Yong Liu,Shuanglin Jiang,Wenyong Li,Rong Wang
Graphical Plasmid DNA coheres to the surface of AuNPs through electrostatic adsorption of the PEI mixture with a certain proportion of high-molecular-weight (25,000) PEI (HMW PEI) and low-molecular-weight (1,800) PEI (LMW PEI). AuNPs−PEI−DNA shows a very low toxic impact to embryo development and excellent efficiency of delivering exogenous DNA into mouse early embryonic cells. A transgenic system of noninvasive cytosolic transport based on exogenous plasmid DNA (pIRES2−EGFP) loading on gold nanoparticles (AuNPs) coated with polyethyleneimine (PEI) was successfully constructed, which can remove the technical threshold and possible damage of microinjection to oocytes and embryo development. In this study, mouse zygotes were cultured in KSOM medium containing the complex of AuNPs−PEI−DNA (APD) until formation of blastocyts, and then transferred into pseudopregnant female mice for obtaining transgenic offspring. Fluorescence observations confirm the expression of GFP in early embryos; importantly, data from identifying experiments by genotyping, immunofluorescence and DNA sequencing show that the GFP sequence indeed integrates into the chromosome of offspring individuals and is expressed. Furthermore, TUNEL test and analysis on cell differentiation of blastocysts indicate an excellent safety of APD to the development of embryos. All these results suggested that AuNPs, being an alternative technique to traditional microinjection, can efficiently introduce biomolecules into early embryonic cells without the prerequisite of expensive micromanipulators and skilled technicians, which will greatly promote study of life sciences in various aspects.
研究领域:
分子生物学
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遗传学
·
生殖医学
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产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2024
·
IF 5.5
Yuhao Zhang,Luting Yan,Lei Tian,Qi Guo,Maosheng Sang,Shouyi Dou,Falan Li,Qingqing Yang,Xia Sun,Yemin Guo,Yanyan Zhang
Vibrio parahaemolyticus ( VP ) is one of the most common pathogens in aquatic products, and its study using electrochemiluminescence is still relatively rare. In this study, an electrochemiluminescent (ECL) biosensor was developed to detect VP in aquatic products using AuNPs modified PEI/MWCNTs (AuNPs@PEI/MWCNTs) as a substrate material and ordered mesoporous carbon (OMC) adsorbed Ru(bpy) 3 2+ (OMC@Ru(bpy) 3 2+ ) as a luminescent material. To increase the conductivity of the biosensor and offer a favorable loading environment for the immobilization of the luminescent probe, AuNPs@PEI/MWCNTs are employed as the substrate. Meanwhile, the OMC@Ru(bpy) 3 2+ functionalized DNA probe acts as a luminescent and capture probe to generate ECL signals and specifically recognize different concentrations of VP . The biosensor has good selectivity and stability and has been successfully applied to the detection of VP in scallops. This method not only expands the potential uses of bioprobe-based ECL biosensors, but also offers a new and effective approach used to detect food-borne pathogens in aquatic products.
研究领域:
食品科学
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生物技术
·
分析化学
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DOI
高影响因子
SENSORS
·
2023
·Vol. 23
·期 13
·6114
·
IF 3.9
Kunhui Sun,Bing Wang,Jiaoli Lin,Lei Han,Meifang Li,Ping Wang,Xiean Yu,Jiangwei Tian
The differences in urinary proteins could provide a novel opportunity to distinguish the different types of drug-induced kidney injury (DIKI). In this research, Au nanoparticles–polyethyleneimine (AuNPs–PEI) and the three fluorophore-labeled proteins (FLPs) have been constructed as a multichannel fluorescent array sensor via electrostatic interaction, which was used to detect the subtle changes in urine collected from the pathological state of DIKI. Once the urine from different types of DIKI was introduced, the binding equilibrium between AuNPs–PEI and FLPs would be broken due to the competitive binding of urinary protein, and the corresponding fluorescence response pattern would be generated. Depending on the different fluorescence response patterns, the different types of DIKI were successfully identified by principal component analysis (PCA) and linear discriminant analysis (LDA). Accordingly, the strategy was expected to be a powerful technique for evaluating the potential unclear mechanisms of nephrotoxic drugs, which would provide a promising method for screening potential renal-protective drugs.Keywords:multichannel fluorescent array sensor;drug-induced kidney injury;Au nanoparticles–polyethyleneimine (AuNPs–PEI);fluorophore-labeled proteins
研究领域:
分析化学
·
生物医学工程
·
肾病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）