BIOCHEMICAL ENGINEERING JOURNAL
·
2016
·
IF 2.463
Xin-Jun Yu,Jie Sun,Ya-Qi Sun,Jian-Yong Zheng,Zhao Wang
In this study, the phytohormone gibberellin was evaluated for its effect on lipid and docosahexaenoic acid (DHA) accumulation in Aurantiochytrium sp.YLH70. It was found that 4 mg/mL of gibberellin increased biomass, lipid and DHA yield of Aurantiochytrium sp.YLH70 by 14.4%, 43.6% and 79.1%, respectively. Moreover, a GC–MS metabolomics method combined with a multivariate analysis was applied to reveal the metabolic mechanisms responsible for the increased lipid and DHA accumulation. The principal component analysis (PCA) revealed that metabolomics profiles from all groups were discriminated. 38 metabolites identified by the partial least-squares-discriminant analysis (PLS-DA) were responsible for responding to gibberellin treatment. Metabolic pathway analysis showed that gibberellin accelerated the rate of utilization of glucose, and metabolites in fatty acids biosynthesis and mevalonate pathway were increased, while metabolites in glycolysis and TCA cycle were decreased in Aurantiochytrium sp.YLH70. Moreover, the anti-stress mechanism in Aurantiochytrium sp.YLH70 might be induced by gibberellin.
研究领域:
生物化学
·
代谢组学
✓ 已验证
DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2015
·Vol. 177
·期 6
·1229-1240
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IF 1.735
Yu Xin-Jun,Yu Zhi-Qiang,Liu Ying-Liang,Sun Jie,Zheng Jian-Yong,Wang Zhao
High-fructose corn syrup (HFCS) is an agro-source product and has been the most commonly used substitute for sugar as sweetener in food industry due to its low price and high solution property. In this study, the F55 HFCS, rich in fructose and glucose, was first tested for biomass and docosahexaenoic acid productions as a mixed carbon source by a newly isolated Aurantiochytrium sp.YLH70. After the compositions of the HFCS media were optimized, the results showed that the HFCS with additions of metal ion and vitamin at low concentrations was suitable for biomass and docosahexaenoic acid productions and the metal ion and sea salt had the most significant effects on biomass production. During the 5-l fed-batch fermentation, total HFCS containing 180 g l −1 reducing sugar was consumed and yields of biomass, lipid, and DHA could reach 78.5, 51, and 20.1 g l −1 , respectively, at 114 h. Meanwhile, the daily productivity and the reducing sugar conversion yield for docosahexaenoic acid were up to 4.23 g l −1 day −1 and 0.11 g g −1 . The fatty acid profile of Aurantiochytrium sp.YLH70 showed that 46.4 % of total fatty acid was docosahexaenoic acid, suggesting that Aurantiochytrium sp.YLH70 was a promising DHA producer.
研究领域:
微生物学
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生物技术
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海洋生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Xin-Jun Yu,Hong Chen,Chang-Yi Huang,Xiao-Yu Zhu,Zhi-Peng Wang,Dong-Sheng Wang,Xiao-Yan Liu,Jie Sun,Jian-Yong Zheng,Hui-Juan Li,Zhao Wang
The phytohormone 6-benzylaminopurine (6-BAP) significantly improves lipid synthesis of oleaginous microorganisms with the great potential applied in lipid production. In the current study, the lipid and DHA productions in oleaginous Aurantiochytrium sp. were found to be improved by 48.7% and 55.3%, respectively, induced by 6-BAP treatments. Then, using high-throughput RNA-seq technology, the overall de novo assembly of the cDNA sequence data generated 53871 unigenes, and 15902 of these were annotated in at least one database. The comparative transcriptomic profiles of cells with and without 6-BAP treatments revealed that a total of 717 were differently expressed genes (DE), with 472 upregulated and 245 downregulated. Further annotation and categorization indicated that some DE genes were involved in pathways crucial to lipid and DHA productions, such as fatty acid synthesis, central carbon metabolism, transcriptional factor, signal transduction, and mevalonate pathway. A regulation mode of 6-BAP, in turn, perception and transduction of 6-BAP signal, transcription factor, expression regulations of the downstream genes, and metabolic changes, respectively, was put forward for the first time in the present study. This research illuminates the transcriptomic mechanism of phytohormone stimulation of lipid and DHA production in an oleaginous microorganism and provides the potential targets modified using genetic engineering for improving lipid and DHA productivity.
研究领域:
微生物学
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分子生物学
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生物技术
·
转录组学
✓ 已验证
DOI
PubMed
高影响因子
Biomass Conversion and Biorefinery
·
2022
·Vol. 13
·期 18
·16807-16818
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IF 4.05
Wang Qing,Jin Wenbiao,Han Wei,Song Kang,Chen Yidi,Chen Chuan,Jiang Guangming,Zhou Xu
In order to achieve strain improvement and reduction of fermentation medium cost, atmospheric and room temperature plasma (ARTP) mutagenesis and microbial microdroplet culture (MMC) high-throughput screening were used to obtain high-yield Aurantiochytrium sp. strain. Meanwhile, low-cost food waste liquid was added into culture medium to reduce cost. The results showed that the optimal treatment time of ARTP mutagenesis for Aurantiochytrium sp. was 25 s, and 20 mutant strains were screened out by MMC to investigate their performance. The most significant increase in DHA yield was mutant Aurantiochytrium sp. MA20, whose biomass, lipid, and DHA yield were 29.0 ± 0.24, 15.8 ± 0.11, and 9.22 ± 0.37 g/L and 5.77, 16.9, and 83.2% higher than those of original strain, respectively. Compared with the original strain, the DHA proportion in total fatty acids of Aurantiochytrium sp. MA20 increased from 37.2 to 58.3%, while the DHA proportion in biomass increased from 18.4 to 31.7%. The genetic stability for Aurantiochytrium sp. MA20 showed that the yield of biomass, lipid, and DHA of the 50th generation were 29.1 ± 0.13, 16.2 ± 0.31, and 9.47 ± 0.26 g/L, respectively, which was no significant difference between original strain, indicating that the mutant MA20 could be inherited stably. Using food waste liquid to replace 100% yeast extract and 50% sea water of the fermentation medium, the DHA yield reached 9.54 g/L, which was equivalent to that of traditional medium, while the cost of medium was reduced by 45% that achieved the recycle of food waste resource. Graphical abstract
研究领域:
微生物学
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生物技术
·
环境科学
✓ 已验证
DOI
高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
·
2015
·Vol. 91
·期 4
·1199-1207
·
IF 2.349
Xin-Jun Yu,Jie Sun,Jian-Yong Zheng,Ya-Qi Sun,Zhao Wang
BACKGROUND Phytohormones are chemical messengers that have a positive effect on biodiesel production of microalgae at low concentrations. However, the effect of phytohormone 6-benzylaminopurine on lipid and docosahexaenoic acid (DHA) production in marine DHA-producer Aurantiochytrium has never been reported. In this study, a GC-MS-based metabolomics method combined with a multivariate analysis is applied to reveal the metabolic mechanism of 6-benzylaminopurine enhancing production of lipid and DHA in Aurantiochytrium sp.YLH70. RESULTS In total, 71 metabolites were identified by GC-MS. The PCA model revealed that 76.9% of metabolite variation was related to 6-benzylaminopurine treatment, and overall metabolomics profiles between the 6-benzylaminopurine and control groups were clearly discriminated. Forty-six metabolites identified by the PLS-DA model were responsible for responding to 6-benzylaminopurine. Metabolic analysis showed that 6-benzylaminopurine could accelerate the rate of utilization of glucose in Aurantiochytrium sp. YLH70, and the metabolic flux from glycolysis, TCA cycle and mevalonate pathway to fatty acids biosynthesis was promoted. Moreover, the anti-stress mechanism in Aurantiochytrium sp.YLH70 might be induced by 6-benzylaminopurine. CONCLUSION Metabolomics is a suitable tool to discover the metabolic mechanism for improving lipid and DHA accumulation in a microorganism. 6-benzylaminopurine has the potential to stimulate lipid and DHA production of Aurantiochytrium sp.YLH70 for industrial purposes. © 2015 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
研究领域:
生物化学
·
海洋生物学
✓ 已验证
DOI
PubMed
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 8
·1593
·
IF 3.06
Xin-Jun Yu,Chang-Yi Huang,Hong Chen,Dong-Sheng Wang,Jing-Liang Chen,Hui-Juan Li,Xiao-Yan Liu,Zhao Wang,Jie Sun,Zhi-Peng Wang
The traditional biochemical methods for analyzing cellular composition of oleaginous microorganisms are time-consuming, polluting, and expensive. In the present study, an FT-IR method was used to analyze the cellular composition of the marine oleaginous protistAurantiochytriumsp. during various research processes, such as strains screening, medium optimization, and fermentation, and was evaluated as a green, low-cost, high throughput, and accurate method compared with the traditional methods. A total of 109Aurantiochytriumsp. strains were screened for lipid and carbohydrate production and the best results were found for the strains No. 6 and No. 32. The yields and productivities could reach up to 47.2 g/L and 0.72 g/L/h for lipid, 21.6 g/L and 0.33 g/L/h for docosahexaenoic acid (DHA) in the strain No. 6, and 15.4 g/L and 0.18 g/L/h for carbohydrate in the strain No. 32, under the optimal conditions, respectively. These results confirmed potentials of the twoAurantiochytriumsp. strains for lipid, DHA, and carbohydrate productions at industrial scales. The FT-IR method in this study will facilitate research on the oleaginousAurantiochytriumsp., and the obtained two strains for lipid and carbohydrate productions will provide the foundations for their applications in medical, food, and feed industries.Keywords:Aurantiochytriumsp., carbohydrate;DHA;feed;FT-IR;high-throughput
研究领域:
生物技术
·
海洋生物学
✓ 已验证
DOI
PubMed
高影响因子
Biomolecules
·
2019
·Vol. 9
·期 11
·695
·
IF 4.694
Dong-Sheng Wang,Xin-Jun Yu,Xiao-Yu Zhu,Zhao Wang,Hui-Juan Li,Zhi-Peng Wang
In the current study, corn steep liquor (CSL) is evaluated as an ideal raw agro-material for efficient lipid and docosahexaenoic acid DHA production byAurantiochytriumsp. Low CSL level in medium (nitrogen deficiency) stimulated the biosynthesis of lipids and DHA while inhibiting cellular growth. The transcriptomic profiles of theAurantiochytriumsp. cells are analyzed and compared when cultured under high (H group), normal (N group), and low (L group) levels of CSL in the medium. The discriminated transcriptomic profiles from the three groups indicates that changes in CSL level in medium result in a global change in transcriptome ofAurantiochytriumsp. The overall de novo assembly of cDNA sequence data generated 61,163 unigenes, and 18,129 of them were annotated in at least one database. A total of 5105 differently expressed (DE) genes were found in the N group versus the H group, with 2218 downregulated and 2887 upregulated. A total of 3625 DE genes were found in the N group versus the L group, with 1904 downregulated and 1721 upregulated. The analysis and categorization of the DE genes indicates that the regulation mechanism of CSL involved in the perception and transduction of the limited nitrogen signal, the interactions between the transcription factors (TFs) and multiple downstream genes, and the variations in downstream genes and metabolites, in sequence, are illuminated for the first time in the current study.Keywords:Aurantiochytriumsp.;nitrogen-deficiency;RNA-seq;corn steep liquor (CSL);signal transduction;DHA
研究领域:
生物技术
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
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